Patents Assigned to Cytonome/ST, LLC
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Publication number: 20120307244Abstract: A microfluidic multiple channel particle analysis system (1) which allows particles (2) from a plurality of particle sources (3) to be independently simultaneously entrained in a corresponding plurality of fluid streams (4) for analysis and sorting into particle subpopulations (5) based upon one or more particle characteristics (6).Type: ApplicationFiled: February 4, 2011Publication date: December 6, 2012Applicant: Cytonome/ST, LLCInventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Blair Morad, Rudolf Hulspas, Donald Francis Perrault, JR.
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Publication number: 20120277902Abstract: Particle processing systems and methods utilize a sort monitoring system to monitor an operational characteristic for a particle sorting system. The operational characteristic may be related to the performance and operation of a sorter or a group of sorters in the particle sorting system. The operational characteristic may be monitored based on monitoring particles for an output of a sorter or of a group of sorters. Operational characteristics which may be monitored include sort error, sort fraction, yield, purity and recovery percentage. The sort monitoring system may evaluate the monitored operational characteristic, for example, as related to sort performance, and take an action, for example, a corrective action or a notifying action, based on the evaluation of the operational characteristic.Type: ApplicationFiled: January 3, 2012Publication date: November 1, 2012Applicant: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Donald F. Perrault, JR., Blair Morad
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Publication number: 20120261013Abstract: A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.Type: ApplicationFiled: June 19, 2012Publication date: October 18, 2012Applicant: CYTONOME/ST, LLCInventors: John Richard Gilbert, Sebastian Böhm, Manish Deshpande
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Patent number: 8277764Abstract: A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.Type: GrantFiled: December 5, 2005Date of Patent: October 2, 2012Assignee: Cytonome/ST, LLCInventors: John R. Gilbert, Hugh Lewis, Derek Beaupre, Jaishree Trikha, Manish Deshpande
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Publication number: 20120202237Abstract: A particle analyzing and/or sorting apparatus and the associated methods. One aspect of the described embodiments relates to an analyzer, or a sorter, having acquisition and sort electronics in the form of a field programmable gate array for processing detected signals. Another aspect relates to a droplet based approach of analyzing and sorting particles and may further include a dynamic element, such a dynamic drop delay. In still another broad aspect, an apparatus and method for dynamically varying other sorting parameters.Type: ApplicationFiled: January 31, 2012Publication date: August 9, 2012Applicant: CYTONOME/ST, LLCInventors: Nemanya Sedoglavich, Stuart Buddy Ehrlich, Emanuel Tito Mendes Machado, Johnathan Charles Sharpe
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Publication number: 20120200857Abstract: A fluid stream imaging apparatus having either optics for manipulating the aspect ratio or sensing elements configured for manipulating the aspect ratio of an image of the fluid stream. This application may also relate to a system for acquiring images of a portion of a fluid stream at high speeds for image processing to measure and predict droplet delays for individual forming particles.Type: ApplicationFiled: February 3, 2012Publication date: August 9, 2012Applicant: CYTONOME/ST, LLCInventors: Johnathan Charles Sharpe, Donald Francis Perrault, JR., Nemanya Sedoglavich
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Patent number: 8210209Abstract: A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.Type: GrantFiled: May 12, 2006Date of Patent: July 3, 2012Assignee: Cytonome/ST, LLCInventors: John R. Gilbert, Sebastian Böhm, Manish Deshpande
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Publication number: 20120138513Abstract: An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed.Type: ApplicationFiled: February 10, 2012Publication date: June 7, 2012Applicant: CYTONOME/ST, LLCInventors: Andrew Johnson, John R. Gilbert, Manish Deshpande, Hugh Lewis, Bernard Bunner
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Patent number: 8123044Abstract: An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed.Type: GrantFiled: May 4, 2007Date of Patent: February 28, 2012Assignee: Cytonome/ST, LLCInventors: Andrew Johnson, John R. Gilbert, Manish Deshpande, Hugh Lewis, Bernard Bunner
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Publication number: 20120015442Abstract: A microfluidic system includes a bubble valve for regulating fluid flow through a microchannel. The bubble valve includes a fluid meniscus interfacing the microchannel interior and an actuator for deflecting the membrane into the microchannel interior to regulate fluid flow. The actuator generates a gas bubble in a liquid in the microchannel when a sufficient pressure is generated on the membrane.Type: ApplicationFiled: September 26, 2011Publication date: January 19, 2012Applicant: CYTONOME/ST, LLCInventors: John Richard Gilbert, Sebastian Böhm, Manish Deshpande
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Publication number: 20120012508Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.Type: ApplicationFiled: September 26, 2011Publication date: January 19, 2012Applicant: CYTONOME/ST, LLCInventors: Manish Deshpande, John Gilbert
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Publication number: 20120006727Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.Type: ApplicationFiled: June 13, 2011Publication date: January 12, 2012Applicant: CYTONOME/ST, LLCInventors: Sebastian Böhm, John R. Gilbert, Manish Deshpande
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Publication number: 20120009025Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.Type: ApplicationFiled: July 8, 2011Publication date: January 12, 2012Applicant: CYTONOME/ST, LLCInventors: John R. Gilbert, Manish Deshpande, Bernard Bunner
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Publication number: 20120009619Abstract: A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.Type: ApplicationFiled: September 22, 2011Publication date: January 12, 2012Applicant: CYTONOME/ST, LLCInventors: John R. Gilbert, Hugh Lewis, Derek Beaupre, Jaishree Trikha, Manish Deshpande
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Patent number: 7997831Abstract: A microfabricated sheath flow structure for producing a sheath flow includes a primary sheath flow channel for conveying a sheath fluid, a sample inlet for injecting a sample into the sheath fluid in the primary sheath flow channel, a primary focusing region for focusing the sample within the sheath fluid and a secondary focusing region for providing additional focusing of the sample within the sheath fluid. The secondary focusing region may be formed by a flow channel intersecting the primary sheath flow channel to inject additional sheath fluid into the primary sheath flow channel from a selected direction. A sheath flow system may comprise a plurality of sheath flow structures operating in parallel on a microfluidic chip.Type: GrantFiled: November 2, 2009Date of Patent: August 16, 2011Assignee: Cytonome/St, LLCInventors: John R. Gilbert, Manish Deshpande, Bernard Bunner
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Publication number: 20110196637Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.Type: ApplicationFiled: February 7, 2011Publication date: August 11, 2011Applicant: CYTONOME/ST, LLCInventors: Johnathan C. Sharpe, Emanuel Tito Mendes Machado, Rudolf Hulspas
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Patent number: 7963399Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.Type: GrantFiled: August 7, 2009Date of Patent: June 21, 2011Assignee: Cytonome/ST, LLCInventors: Sebastian Böhm, John R. Gilbert, Manish Deshpande
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Patent number: 7870964Abstract: A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. An infusion pump for delivering a fluid from a fluid source may be integrated in a microfluidic chip using a capping module having pumping components formed therein.Type: GrantFiled: November 24, 2008Date of Patent: January 18, 2011Assignee: Cytonome / St, LLCInventors: John R. Gilbert, Manish Deshpande
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Publication number: 20110005978Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.Type: ApplicationFiled: June 23, 2010Publication date: January 13, 2011Applicant: Cytonome/ST, LLCInventors: Sebastian Böhm, John R. Gilbert, Manish Deshpande
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Publication number: 20100133151Abstract: A method and apparatus for sorting particles moving through a closed channel system of capillary size comprises a bubble valve for selectively generating a pressure pulse to separate a particle having a predetermined characteristic from a stream of particles. The particle sorting system may further include a buffer for absorbing the pressure pulse. The particle sorting system may include a plurality of closely coupled sorting modules which are combined to further increase the sorting rate. The particle sorting system may comprise a multi-stage sorting device for serially sorting streams of particles, in order to decrease the error rate.Type: ApplicationFiled: August 7, 2009Publication date: June 3, 2010Applicant: CYTONOME/ST, LLCInventors: Sebastian Bohm, John R. Gilbert, Manish Deshpande