Patents Assigned to Dako
  • Patent number: 5985563
    Abstract: PNA probes for detection of Neisseria gonorrhoeae and Chlamydia trachomatis.Specific peptide nucleic acid (PNA) probes for detecting a sexual transmitted disease caused by Neisseria gonorrhoeae or Chlamydia trachomatis comprising N-(2-aminoethyl)glycine units in amide linkage with the glycine nitrogen connected to naturally occurring nucleobases or non-naturally occurring nucleobases by a methylene carbonyl linker and said probes capable of hybridizing to 16S or 23S rRNA or rDNA of Neisseria gonorrhoeae or Chlamydia trachomatis are described.PNA is a very stable molecule with very high affinity for nucleic acid allowing a PNA probe to be shorter than conventional nucleic acid probes.
    Type: Grant
    Filed: June 5, 1997
    Date of Patent: November 16, 1999
    Assignee: Dako A/S
    Inventors: Jens J.o slashed.rgen Hyldig-Nielsen, Michael Anders Godskesen
  • Patent number: 5888733
    Abstract: Methodologies for determining the presence of specific nucleic acid sequences in a sample of eucaryotic origin using in situ hybridization are described. The in situ hybridization is performed using a hybridization solution comprising at least one binding partner capable of hybridizing to the specific nucleic acid sequence to be determined so as to form hybrids and a hybrid destabilizing agent in an amount effective to decrease the melting temperature of hybrids formed between the nucleic acid and the binding partner so as to increase the specific binding and decrease the non-specific binding. The binding partner used is a polymeric strand of polymerized moieties having a non-cyclic backbone, the polymeric strand being capable of hybridizing to the nucleic acid sequence to be determined. The method is particularly suitable for diagnosing different human diseases such as bacterial and viral infections, genetic diseases and neoplastic disorders.
    Type: Grant
    Filed: October 2, 1996
    Date of Patent: March 30, 1999
    Assignee: Dako A/S
    Inventors: Jens J. Hyldig-Nielsen, Karl-Johan Pluzek, Tom Just
  • Patent number: 5804404
    Abstract: The preparation and use of stable, ready-to-use substrate-chromogen solutions for the detection and quantification of peroxidase or pseudoperoxidase activity are described. The substrate-chromogen solution according to the present invention contains a buffer, preferably imidazole buffer, a polymer, a hydroperoxide substrate, a chromogenic organic electron donor and electron donor of the carbohydrate type as a stabilizer. The substrate-chromogen solution retains its full peroxidatic activity and develops a negligible color during storage.
    Type: Grant
    Filed: January 22, 1996
    Date of Patent: September 8, 1998
    Assignee: Dako Corporation
    Inventors: Alfonso Heras, Marc Key
  • Patent number: 5612458
    Abstract: This invention relates to antibodies to complexes formed between PNA (Peptide Nucleic Acid) and nucleic acids, particularly antibodies to PNA/DNA or PNA/RNA complexes. The preferred antibodies are polyclonal, monoclonal and recombinant antibodies that binds to PNA/DNA or PNA/RNA complexes, but not to single-stranded PNA, double-stranded nucleic acid or single-stranded nucleic acid. Peptide Nucleic Acids (PNA) are newly developed, not naturally occurring compounds comprising a polyamide backbone bearing a plurality of ligands such as naturally occuring nucleobases attached to a polyamide backbone through a suitable linker. PNA oligomers with a backbone of N-(2-aminoethyl)glycin units have a surprising high affinity for complementary nucleic acid forming very stable and specific complexes. This property makes PNA oligomers suitable as hybridization probes for detection of nucleic acids. The usability of PNA as hybridization probes is greatly increased by the present antibodies.
    Type: Grant
    Filed: December 22, 1994
    Date of Patent: March 18, 1997
    Assignee: Dako/AS
    Inventors: Jens J. Hyldig-Nielsen, Karl-Johan Pluzek
  • Patent number: 5289140
    Abstract: Use of microwave equipment for fast food preparation and for speeding up chemical reactions e.g. in diagnostic testing is wellknown. However in all such uses the known equipment provides a very uneven heating effect, thereby limiting the value of the method. The present patent application describes an improved type of microwave equipment, in which an even effect is obtained by the use of a combination of microwaves of different wavelengths. The equipment according to the invention includes one or more microwave sources, controlled individually or jointly, emitting microwaves of a two or more wavelengths, e.g. in the form of distinct single wavelengths, White Noise, Square Waves.
    Type: Grant
    Filed: August 16, 1991
    Date of Patent: February 22, 1994
    Assignee: Dako Japan Co., Ltd.
    Inventors: Finn Jorgenson, Bent B. Vaboe
  • Patent number: 4586360
    Abstract: Fine die stamping of workpieces from sheet metal is effected by clamping the workpiece between a die plate and guide plate, supporting the portion of the material to form the article by an ejector which is displaced along with the punch, and driving the supported portion of the material into a hole in the die plate having flanks tapered away from the guide plate and punch so that swaging occurs on the separated portion with or upon its separation from the balance of the workpiece material.
    Type: Grant
    Filed: July 6, 1984
    Date of Patent: May 6, 1986
    Assignee: Dako-Werkzeugfabriken David Kotthaus GmbH & Co. KG
    Inventors: Willi Jurgensmeyer, Friedrich-Wilhelm Honsberg, Kurt Halbach