Patents Assigned to Dako
  • QUANTIFICATION OF SINGLE TARGET MOLECULES IN HISTOLOGICAL SAMPLES
    Publication number: 20140038169
    Abstract: The present invention lies in the field of visualization and quantification of immobilized targets in samples using immunochemical means. The methods of the invention utilize an immunostaining system allowing visualizing single target units in samples as distinct dots. In particular, the invention relates to methods and reagents for visualization and quantification of molecular targets immunostained in histological samples and use of said method and reagents in medical diagnostic. However, the visualization and quantification methods of the invention are applicable to a variety of targets in different samples and allow precise quantifying both relative and absolute amounts thereof.
    Type: Application
    Filed: November 8, 2011
    Publication date: February 6, 2014
    Applicant: DAKO DENMARK A/S
    Inventors: Jesper Lohse, Galina Skladtchikova
  • Methods and compositions for immuno-histochemical detection
    Patent number: 8632994
    Abstract: The invention relates to methods and compositions of detecting a biological marker in a sample. In certain embodiments, the methods and compositions comprise a first binding agent that specifically binds to a biological marker in a sample; a second binding agent linked to a first polymer, wherein the second binding agent specifically binds to the first binding agent; a third binding agent linked to a second polymer, wherein the third binding agent specifically binds to the second binding agent; and a detectable substance. In certain embodiments the biological markers are detected immunologically.
    Type: Grant
    Filed: November 30, 2004
    Date of Patent: January 21, 2014
    Assignee: Dako Denmark A/S
    Inventors: Lars Winther, Anthony Lionel Knoll, Kellyann Weckworth, Uffe Lovborg
  • COMBINED HISTOLOGICAL STAIN
    Publication number: 20130337441
    Abstract: The present invention relates to methods of visualizing targets in histological samples, e.g. biopsy samples, wherein the methods comprise staining of the sample with (i) one or more target specific immunochemical stains, and (ii) a histological stain for specific tissue components e.g. iron, mucins glycogen, amyloid, nucleic acids, etc., e.g. hematoxilyn and/or eosin stains or the like, that is used to enhance contrast in the microscopic image of a tissue sample, highlight morphologic structures in the sample for viewing, define and examine tissues, cell populations, or organelles within individual cells. Methods may further comprise evaluation of expression of one or more targets in the sample. The disclosed methods are useful for medical diagnostics.
    Type: Application
    Filed: December 6, 2011
    Publication date: December 19, 2013
    Applicant: DAKO DENMARK A/S
    Inventor: Jesper Lohse
  • Monomeric and polymeric linkers useful for conjugating biological molecules and other substances
    Patent number: 8546309
    Abstract: The present invention concerns monomeric or polymeric linker molecules useful in biological and chemical applications, their synthesis, and the synthesis and use of derivatives of the linkers conjugated to a variety of detectable labels and other substances. The linkers may be used, for example, in conjunction with fluorescent labels, nucleic acid or nucleic acid analog probes, and solid phase systems, and to enhance the solubility of the conjugated molecules.
    Type: Grant
    Filed: June 30, 2006
    Date of Patent: October 1, 2013
    Assignee: Dako Denmark A/S
    Inventor: Jesper Lohse
  • Cell counting
    Patent number: 8541227
    Abstract: The inventions relates to compositions and method for determining the absolute counts of cells per unit volume of a sample. Such a method comprises: (a) providing a container containing (i) a predetermined quantity of microparticles; and (ii) a cell-binding agent; in which the microparticles are disposed in or on a matrix which adheres to at least one wall of the container such that substantially all the microparticles are thereby attached to the container; (b) adding a known volume of sample to the container; (c) determining the ratio of microparticles to cells by counting microparticles and cells in a volume of the sample; and (d) determining the absolute count of cells by multiplying the number of cells per microparticle by the concentration of microparticles in the sample.
    Type: Grant
    Filed: February 6, 2006
    Date of Patent: September 24, 2013
    Assignee: Dako Denmark A/S
    Inventors: Nanna K Christensen, Jesper Laursen, Lars Winther
  • Apparatus for automated processing biological samples
    Patent number: 8529836
    Abstract: The present invention concerns an apparatus for automatic processing at least one biological sample accommodated on a carrier member, such as a slide by applying a predetermined amount of reagents in a predetermined sequence according to a processing protocol, said apparatus comprising; a housing frame; at least one processing section for accommodating at least one slide, the at least one processing section is provided within the housing; a hood cover protecting the at least one processing section in said housing; wherein the hood cover completely encloses the processing section defining an interior space; and wherein the apparatus further comprises climate control device provided to control the environment within the interior space.
    Type: Grant
    Filed: June 11, 2012
    Date of Patent: September 10, 2013
    Assignee: DAKO Denmark A/S
    Inventors: Lars Winther, Marc Key, Kristopher Buchanan, John Favuzzi, Benno Guggenheimer
  • Methods and Compounds For Detection of Molecular Targets
    Publication number: 20130230853
    Abstract: The present invention relates to methods and compounds for detection of molecular targets, such as biological or chemical molecules, or molecular structures, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc.
    Type: Application
    Filed: April 4, 2013
    Publication date: September 5, 2013
    Applicant: DAKO Denmark A/S
    Inventor: Jesper Lohse
  • ENHANCED SCHEDULING SAMPLE PROCESSING SYSTEM AND METHODS OF BIOLOGICAL SLIDE PROCESSING
    Publication number: 20130217108
    Abstract: A sample processing system 101 that may be automated and methods are disclosed where sample(s) 198 are arranged on a carrier element 197 and a process operation control system 171 automatically processes the sample(s) perhaps robotically according to an desired aggregation of event dictated by an input 173. Alteration of an initial aggregated event topology may be accepted while the system is processing an initial aggregation and varied-parameter robotic control simulation functionalities 606 may be accomplished to determine an enhanced sequence for processing. Suggested operator actions may be displayed that might further enhance the scheduling of the altered aggregated event topology together with an automatic operator need prompt 608 that may inform an operator of a need for a particular action in order to accomplish the desired tasks.
    Type: Application
    Filed: February 4, 2013
    Publication date: August 22, 2013
    Applicant: DAKO DENMARK A/S
    Inventor: DAKO DENMARK A/S
  • Human papilloma virus probes for the diagnosis of cancer
    Patent number: 8513398
    Abstract: In one embodiment, the invention relates to a method of detecting cervical cancer, and other types of cancer, using a combination of at least three genomic clones, or fragments thereof, of high risk Human Papilloma Virus. For example, the invention relates to a composition comprising at least three full length genomic clones, or fragments thereof, of high risk Human Papilloma Viruses.
    Type: Grant
    Filed: June 19, 2012
    Date of Patent: August 20, 2013
    Assignee: Dako Denmark A/S
    Inventors: Miu Fun Chau, Kirsten Bisgaard-Franzen, Jone Lin, Ole Feldballe Rasmussen, Zunde Wang, Jason Lusk, Martin Lindberg, Sienna Yoast
  • INFORMATION NOTIFICATION SAMPLE PROCESSING SYSTEM AND METHODS OF BIOLOGICAL SLIDE PROCESSING
    Publication number: 20130203103
    Abstract: A sample processing system that may be automated and methods are disclosed where samples are arranged on a carrier element and a process operation control system automatically processes the samples perhaps robotically with an operationally-influential exteriorly-consequential information monitor or a data capture element. Significant process details as well as operationally-influential exteriorly-consequential information may be monitored and an automatic notice element may cause notification of a person at some display that may be remote. Various people may be notified, such as an administrator, a supplier, or a manufacturer of an opportunity for some action such as reagent reordering or the like. A simulated motion display may be included to “watch” simulated operation in real time or long after completion of the actual processing.
    Type: Application
    Filed: January 23, 2013
    Publication date: August 8, 2013
    Applicant: DAKO DENMARK A/S
    Inventor: DAKO Denmark A/S
  • Holding device for microscope slides with tissue specimens
    Patent number: 8498049
    Abstract: A holding device for retaining a number of slides with tissue specimens is described. The holding device includes an elongated hanger having a clamping mechanism for releasable clamping of the microscope slides at their upper ends and for retaining the microscope slides in a hanging position with the surfaces of the microscope slides in said a common plane. The clamping mechanism preferably is arranged to cooperate with a loading rack having a supporting surface for support of the microscope slides in the common plane, the hanger being able to be introduced into the rack and the clamping mechanism being able to be actuated so that all microscope slides placed on the supporting surface are clamped in the holding device.
    Type: Grant
    Filed: April 25, 2011
    Date of Patent: July 30, 2013
    Assignee: Dako Instrumec AS
    Inventors: Oystein Ljungmann, Torstein Ljungmann
  • APPARATUS FOR EXECUTION OF TREATMENT OPERATIONS IN CONNECTION WITH COLOURING OF TISSUE SPECIMENS ON OBJECT GLASSES
    Publication number: 20130183708
    Abstract: An apparatus for automatic execution of different treatment operations in connection with staining of tissue specimens on microscope slides, wherein the apparatus (1) comprises a loading station (2) for microscope slides (7) with tissue specimens, a number of reagent stations (3) for staining of the tissue specimens on supp Ned microscope slides, a conveyor (5) for transfer of microscope slides (7) between the stations (3) in accordance with a staining program, an unloading station (10) for treated microscope slides, and a control unit (19) for controlling the treatment operations in accordance with a data program, The apparatus comprise a photo station (25) with a digital camera (26) for automatic photographing with a background light source of the finished treated tissue specimens on supplied microscope slides (7).
    Type: Application
    Filed: March 5, 2013
    Publication date: July 18, 2013
    Applicant: DAKO INSTRUMEC AS
    Inventor: DAKO INSTRUMEC AS
  • Reagent delivery system, dispensing device and container for a biological staining apparatus
    Patent number: 8486714
    Abstract: The invention concerns reagent delivery system for an apparatus for processing of biological samples arranged on microscope slides, comprising a reagent section having one or more reagent containers; a slide section in which at least one microscope slide is arranged; a probe for dispensing a portion of reagent onto a predetermined microscope slide, and means for handling the probe. The probe comprises a continuous prove tubing element extending through a rigid probe member and connecting the probe tip to a pneumatic pressure regulation device. The reagent containers are adapted for cooperation with the probe tip. In this manner a high though-put and a very low carry over of fluid residues is achieved since there is no assembled parts making up the inside volume of the probe in which the fluid may be retained.
    Type: Grant
    Filed: April 28, 2008
    Date of Patent: July 16, 2013
    Assignee: DAKO Denmark A/S
    Inventors: John A Favuzzi, Merritt M Martin, Jonathan R Sweda, Lauren Bland, Robert L Lathrop, John E Van Bosch, Kristopher S Buchanan
  • Nucleic acid base pairs
    Patent number: 8481697
    Abstract: The invention relates to non-natural bases and base pairs that expand the normal DNA-based encoding system. Compositions herein may comprise at least one non-natural base that may interact with another base via a Watson Crick-type hydrogen bonding geometry and/or a Hoogsteen-type hydrogen bonding geometry. The bases may be used in a molecular entity, such as an oligomer or any other entity wherein the bases are attached to a backbone. For example, they may be comprised in DNA, RNA, or PNA, or a variety of other nucleic acid-type systems.
    Type: Grant
    Filed: June 30, 2006
    Date of Patent: July 9, 2013
    Assignee: Dako Denmark A/S
    Inventors: Jesper Lohse, Kenneth Heesche Petersen
  • SYSTEMS AND METHODS FOR TRACKING AND PROVIDING WORKFLOW INFORMATION
    Publication number: 20130166345
    Abstract: A tangible computer-readable storage device storing computer-executable program instructions that generate a user interface for displaying workflow information associated with a tissue specimen in a pathology laboratory. The program instructions may be configured to perform a method including displaying a virtual laboratory component representing a physical pathology laboratory having one or more laboratory stations for processing the tissue specimen, wherein the tissue specimen is processed by the one or more laboratory stations according to a workflow, and displaying a specimen indicator that indicates a current specimen state based on a current relationship of the tissue specimen to the workflow.
    Type: Application
    Filed: November 30, 2012
    Publication date: June 27, 2013
    Applicant: DAKO DENMARK A/S
    Inventor: DAKO DENMARK A/S
  • Horizontal antigen retrieval
    Patent number: 8465944
    Abstract: The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.
    Type: Grant
    Filed: February 11, 2008
    Date of Patent: June 18, 2013
    Assignee: Dako Instrumec AS
    Inventors: Nanna K. Christensen, Lars Winther
  • Methods and compounds for detection of molecular targets
    Patent number: 8435735
    Abstract: The present invention relates to methods and compounds for detection of molecular targets, such as biological or chemical molecules, or molecular structures, in samples using a host of experimental schemes for detecting and visualizing such targets, e.g. immunohistochemistry (IHC), in situ hybridization (ISH), ELISA, Southern, Northern, and Western blotting, etc.
    Type: Grant
    Filed: February 19, 2010
    Date of Patent: May 7, 2013
    Assignee: Dako Denmark A/S
    Inventor: Jesper Lohse
  • Systems and Methods of Sample Processing and Temperature Control
    Publication number: 20130084567
    Abstract: Systems and methods of sample processing and temperature control are disclosed. The invention may especially relate to temperature control, and may in some embodiments be methods of temperature control of an automated sample processing system and methods of automated sample processing. Specifically, the present invention provides temperature control in relation to sample processing systems and methods of processing samples, and in some embodiments provides temperature control in relation to sample carriers and processing materials such as reagents. Corresponding systems and devices are disclosed, including sample processing systems (1), sample carrier temperature regulation systems (60), reagent temperature regulation systems, sample processing control systems, and temperature regulation devices, among other embodiments.
    Type: Application
    Filed: September 24, 2012
    Publication date: April 4, 2013
    Applicant: DAKO DENMARK A/S
    Inventor: DAKO DENMARK A/S
  • Apparatus for execution of treatment operations in connection with colouring of tissue specimens on object glasses
    Patent number: 8409506
    Abstract: An apparatus for automatic execution of different treatment operations in connection with staining of tissue specimens on microscope slides, wherein the apparatus (1) comprises a loading station (2) for microscope slides (7) with tissue specimens, a number of reagent stations (3) for staining of the tissue specimens on supplied microscope slides, a conveyor (5) for transfer of microscope slides (7) between the stations (3) in accordance with a staining program, an unloading station (10) for treated microscope slides, and a control unit (19) for controlling the treatment operations in accordance with a data program. The apparatus comprise a photo station (25) with a digital camera (26) for automatic photographing with a background light source of the finished treated tissue specimens on supplied microscope slides (7).
    Type: Grant
    Filed: October 17, 2012
    Date of Patent: April 2, 2013
    Assignee: Dako Instrumec AS
    Inventors: Oystein Ljungmann, Torstein Ljungmann
  • Enhanced scheduling sample processing system and methods of biological slide processing
    Patent number: 8394635
    Abstract: A sample processing system 101 that may be automated and methods are disclosed where sample(s) 198 are arranged on a carrier element 197 and a process operation control system 171 automatically processes the sample(s) perhaps robotically according to an desired aggregation of event dictated by an input 173. Alteration of an initial aggregated event topology may be accepted while the system is processing an initial aggregation and varied-parameter robotic control simulation functionalities 606 may be accomplished to determine an enhanced sequence for processing. Suggested operator actions may be displayed that might further enhance the scheduling of the altered aggregated event topology together with an automatic operator need prompt 608 that may inform an operator of a need for a particular action in order to accomplish the desired tasks.
    Type: Grant
    Filed: May 6, 2011
    Date of Patent: March 12, 2013
    Assignee: DAKO Denmark A/S
    Inventors: Marc Key, Gordon Feingold, Rosanne Welcher