Abstract: The present invention relates to a nucleic acid which is suitable for evaluating the progression potential of cervial lesions, wherein the nucleic acid is obtainable by a process in which RNA from early and late passages of HPV-immortalized cells is isolated and the RNAs characteristic for the early passages and late passages, respectively, are identified and provided as DNA or RNA. Furthermore, the present invention concerns polypeptides coded by such a nucleic acid. In addition, it covers antibodies directed against the polypeptides. Moreover, it relates to the use of the DNA and the polypeptides as well as a kit suitable for evaluating the progression of cervical lesions.
Type:
Grant
Filed:
September 3, 1999
Date of Patent:
May 14, 2002
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen
Rechts
Abstract: A phagemid has been constructed that expresses an antibody fused to coliphage pIII protein. The phagemid is suitable for selecting specific antibodies from large gene libraries with small quantities of antigen. The antibody-pIII gene can be strongly repressed, so that it allows antibody libraries to be amplified without the danger of deletion mutants predominating. After induction, large quantities of the fusion protein may be expressed.
Type:
Grant
Filed:
August 25, 2000
Date of Patent:
May 14, 2002
Assignee:
Deutsches Krebsforschungszentrum Stiftung des
öffentlichen Rechts
Inventors:
Frank Breitling, Melvyn Little, Stefan Dübel, Michael Braunagel, Iris Klewinghaus
Abstract: The invention relates to a pharmaceutical composition containing an inhibitor for diaphragm pumps and a photoaffinity-marked chemotherapeutic agent. Said composition is suitable for eliminating membrane-mediated cell resistance, especially membrane-mediated multi-chemoresistance of tumor cells.
Type:
Grant
Filed:
March 3, 2000
Date of Patent:
April 23, 2002
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen
Rechts
Inventors:
Christoph Granzow, Herwig Ponstingl, Irmgard Hefft, Marijana Kopun-Granzow, Gabriele Gros, Michael Stöhr
Abstract: The present invention relates to p53 binding regions on a CD95 receptor DNA and to the application of the p53 binding regions to influence apoptosis and/or identify suitable substances therefor.
Type:
Application
Filed:
April 12, 2001
Publication date:
April 11, 2002
Applicant:
Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts
Inventors:
Peter Krammer, Martina Muller-Schilling, Moshe Oren
Abstract: The present invention is, generally, directed to the use of betulinic acid and derivatives thereof for the treatment of neuroectodermal tumors. The present invention is based on the discovery that betulinic acid and its derivatives are potent anti-neuroectodermal agents. As disclosed herein, betulinic acid and its derivatives are useful for the treatment of neuroectodermal tumors, including, due to its distinct mechanism of action, neuroectodermal tumors that are resistant to conventional chemotherapeutical agents. In addition to the new use of known compounds, the invention discloses novel compounds and pharmaceutical compositions for the treatment of neuroectodermal tumors.
Type:
Grant
Filed:
October 28, 1998
Date of Patent:
April 9, 2002
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen
Rechts
Inventors:
Klaus Michael Debatin, Simone Fulda, Manfred Wiessler, Marek Los, Walter Mier
Abstract: This invention relates to a DNA coding for a peptide of a papilloma virus major capsid protein. Moreover, this invention deals with a papilloma virus genome containing such a DNA. Furthermore, this invention concerns proteins coded by the papilloma virus genome and virus-like particles as well as antibodies directed thereagainst and the use thereof for diagnosis, treatment and vaccination.
Type:
Grant
Filed:
May 25, 2000
Date of Patent:
April 9, 2002
Assignee:
Deutsches Krebsforschungszentrum Stiftung des
Öffentlichen Rechts
Inventors:
Vladimir Shamanin, Ethel-Michele De Villiers-Zur Hausen, Harald Zur Hausen
Abstract: The present invention relates to a protein containing an SRCR domain, a nucleic acid encoding such a protein and a method to produce same. The invention further relates to the use of the nucleic acid and the protein and antibodies directed against the protein.
Type:
Grant
Filed:
August 31, 1999
Date of Patent:
February 12, 2002
Assignee:
Deutsches Krebsforschungszentrum Stiftung des offentlichen
Rechts
Abstract: The present invention relates to a vector suitable for use in gene therapy, which comprises an expressible insert DNA which codes for the DNA binding domain of a poly(ADP-ribose)-polymerase or for an at least partially catalytically inactive poly(ADP-ribose)-polymerase. Furthermore, this invention concerns processes for the preparation of such a vector and viruses suitable for use in gene therapy.
Type:
Grant
Filed:
October 3, 1997
Date of Patent:
January 1, 2002
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen
Rechts
Inventors:
Alexander Bürkle, Harald Zur Hausen, Küpper Jan-Heiner
Abstract: The invention relates to a device for detecting input radiation such as X-rays, &ggr;-rays, ionizing radiation or fluorescent and low-level light. The device has at least one detection element comprising a sensor component (scintillator) for converting the input radiation into photons (scintillation light) in the UV, visible or IR part of the electromagnetic spectrum and an optical amplifier component which receives the light converted by the sensor component, forwards it for further processing and amplifies it at the same time. According to the invention, the amplifier component comprises at least one optical waveguide (1, 2, 2a, 2b, 20) whose material is optically pumped to amplify the scintillation light (3, 24, 25).
Type:
Grant
Filed:
March 26, 1999
Date of Patent:
October 30, 2001
Assignee:
Deutsches Krebsforschungszentrum Stiftung Des Offentlichen
Rechts
Inventors:
Karlheinz Gross, Wolfgang Maier-Borst, Michael Klaus, Hans-Hermann Schrenk, Gerd Stehle, Hannsjörg Sinn
Abstract: The present invention relates to conjugates comprising a compound capable of emitting fluorescence, a linker and a protein. The present invention also concerns the preparation of such conjugates as well as their use.
Type:
Grant
Filed:
November 19, 1998
Date of Patent:
October 23, 2001
Assignee:
Deutsches Krebsforschungszentrum Stiftung des offentlichen
Rechts
Inventors:
Hansjörg Sinn, Wolfgang Maier-Borst, Gerd Stehle, Michael Kaus, Andreas Wunder, Hans-Hermann Schrenk
Abstract: This invention relates to a conjugate which consists of an active substance and a native protein which is not regarded as exogenous and distinguishes itself in that an intracellularly cleavable linker is present between the active substance and the protein.
Type:
Application
Filed:
April 4, 2001
Publication date:
August 9, 2001
Applicant:
Deutsches Krebsforschungszentrum stiftung des Offentlichen Rechts
Inventors:
Hansjorg Sinn, Hans-Hermann Schrenk, Wolfgang Maier-Borst, Gerd Stehle, Andreas Wunder, Dirk Hoff-Biederbeck, Dieter Ludwig Heene
Abstract: The radiation system has several radiation sources aimed toward a central point. These radiation sources are on arc element that pivots on an axis. The center can hence be irradiated from different sides. The radiation sources preferably have irregularly adjustable diaphragms. The system is suitable for irradiating any part of the human body. It is easy to manufacture and simple to used.
Type:
Grant
Filed:
August 23, 2000
Date of Patent:
July 10, 2001
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen
Rechts
Inventors:
Otto Pastyr, Wolfgang Schlegel, Simone Barthold, Karl-Heinz Höver, Gernot Echner
Abstract: The present invention relates to a preparation comprising a substance influencing the cellular redox state for treating papilloma virus-positive malignant lesions.
Type:
Grant
Filed:
September 4, 1997
Date of Patent:
May 29, 2001
Assignee:
Deutsches Krebsforschungszentrum Stiftung Des
Öffentlichen Rechts
Abstract: The present invention relates to a method of detecting specific antibodies directed against HPV proteins in body fluids, comprising the following steps:
(I) incubating a native carrier material-bound HPV protein with body fluids, and
(II) reacting specific antibodies (a) bound to the HPV protein
with labeled antibodies (b) directed against antibodies (a) or
with unlabeled antibodies (b) and the latter with labeled antibodies (c) directed against antibodies (b).
Furthermore, this invention concerns a kit usable for this purpose.
Type:
Grant
Filed:
March 9, 1998
Date of Patent:
April 10, 2001
Assignee:
Deutsches Krebsforschungszentrum Stiftung Des Offentlichen
Rechts
Inventors:
Hanswalter Zentgraf, Manfred Frey, Iris Velhagen, Regina Martens, Wolfgang Meschede, Michael Pawlita, Joris Braspenning, Masimo Tommasino
Abstract: The invention concerns metallocene-phosphoramidite conjugates comprising one or a plurality of metallocenes and one or a plurality of phosphoramidites. The invention further concerns a process for preparing the metallocene-phosphoramidite conjugates and their use.
Type:
Grant
Filed:
October 21, 1998
Date of Patent:
April 3, 2001
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen
Rechts
Abstract: A contour collimator has a plurality of plate-shaped diaphragm elements movably arranged with respect to each other in a guiding block to form a contour diaphragm for a radiation beam emitted by a radiation source towards the collimator, and at least one drive for moving the diaphragm elements. A drive is associated with each diaphragm element with the drives of a group of diaphragm elements being substantially adjacent, and a driving transmission arranged between each drive and the associated diaphragm element.
Type:
Grant
Filed:
May 15, 1998
Date of Patent:
February 13, 2001
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen
Rechts
Inventors:
Otto Pastyr, Wolfgang Schlegel, Karl-Heinz Hover, Wolfgang Maier-Borst
Abstract: The present invention relates to a process for preparing acid amides by reacting an acid with an aliphatic amine in molten urea. This invention also concerns a process for metalating compounds which can be bonded to a metal ion by reacting the compound with a metal ion in molten urea. Furthermore, the invention deals with the thus obtained products and their use for the therapy and/or diagnosis of tumors or inflammatory diseases.
Type:
Grant
Filed:
December 6, 1999
Date of Patent:
January 23, 2001
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen
Rechts
Inventors:
Hannsjörg Sinn, Wolfgang Maier-Borst, Hans-Hermann Schrenk, Gerd Stehle
Abstract: The present invention relates to a process for quantitatively determining CD95 ligand, comprising the following processing steps:(a) isolation of total RNA from cells,(b) transcription the total RNA from (a) into cDNA by reverse transcription, and(c) amplification of the cDNA from (b) and a CD95 ligand competitor fragment by CD95 ligand-specific primers in a PCR reaction.The process is suitable for determining the extent and/or course of apoptosis.
Type:
Grant
Filed:
October 21, 1998
Date of Patent:
November 28, 2000
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
Abstract: A phagemid has been constructed that expresses an antibody fused to coliphage pIII protein. The phagemid is suitable for selecting specific antibodies from large gene libraries with small quantities of antigen. The antibody-pIII gene can be strongly repressed, so that it allows antibody libraries to be amplified without the danger of deletion mutants predominating. After induction, large quantities of the fusion protein may be expressed.
Type:
Grant
Filed:
October 7, 1999
Date of Patent:
October 3, 2000
Assignee:
Deutsches Krebsforschungszentrum Stiftung des offentlichen Rechts
Inventors:
Frank Breitling, Melvyn Little, Stefan Dubel, Michael Braunagel, Iris Klewinghaus
Abstract: The invention relates to a method of detecting p53-specific antibodies in body fluids wherein support-bound p53 and/or support-bound fragments thereof comprising binding regions for p53-specific antibodies are incubated with body fluids and the specific antibodies (a) bound to p53 and/or said fragments are allowed to reactwith labelled antibodies (b) directed against antibodies (a), orwith unlabelled antibodies (b) while the latter are allowed to react with labelled antibodies (c) directed against antibodies (b), with the labelling being non-radioactive in either case.The invention further relates to a kit useful therefor. Also, the invention relates to p53 fragments and DNA sequences coding for them, said fragments comprising binding regions for p53-specific antibodies, as well as to methods of preparing them.
Type:
Grant
Filed:
May 30, 1994
Date of Patent:
April 18, 2000
Assignee:
Deutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts
Inventors:
Hanswalter Zentgraf, Peter Schranz, Martin Volkmann, Claudia Tessmer, Ralf Klein