Abstract: The invention relates to an interacting polypeptide consisting of or comprising a heptapeptide pattern of sequence X1X2X3X4X5X6X7 and a transduction domain, characterized in that it is a chimera polypeptide, the amino acid X7 is located between 5 and 35 amino acids of the C-terminal end of said polypeptide, and that the domain (b) is situated in C-terminal relative to pattern (a). The invention also relates to screening methods for identifying interacting polypeptides capable of modifying the phenotype of a cell and to uses of interacting polypeptides as mentioned in phenotypic screens or for therapeutic purposes. Lastly, the invention concerns interacting polypeptides capable of modifying the function of the HIV-1 Rev viral protein.
Type:
Grant
Filed:
March 30, 2006
Date of Patent:
May 4, 2010
Assignees:
Ecole Normale Superieure de Lyon, Centre National de la Recherche Scientifique
Inventors:
Pierre Jalinot, Armelle Roison, Jean-Philippe Robin
Abstract: The invention concerns modified avian ES cells, specifically expressing an exogenous gene when they have a pluripotent character. The invention also concerns a nucleic acid and a polypeptide specifically expressed in pluripotent avian cells, and methods for detecting the pluripotent character of cells using said nucleic acid and polypeptide.
Type:
Application
Filed:
November 21, 2007
Publication date:
June 11, 2009
Applicants:
INSTITUT NATIONAL DE LA RESEARCHE AGRONOMIQUE, ENS-ECOLE NORMALE SUPERIEURE DE LYON, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
Abstract: The invention concerns a device for microstructuring the surface of at least one substrate characterized in that it comprises a support frame (2) including at least means (6) for conveying the substrate (S) comprising preferably means (15) for supporting the substrate adapted to maintain the substrate in or relative to each of the modules so as to avoid any change of support when the substrate is shifted from one module to the other, a module for applying a photosensitive resin layer on the substrate, a module (20) for automatic exposure using a laser beam of the resin layer on the substrate based on a programmed scheme, a so-called developing module for removing the part of the resin layer which has been exposed of the part or the resin layer which has not been exposed, a module for chemically etching the substrate, a module for cleaning the substrate, and a control unit (7).
Type:
Application
Filed:
July 1, 2005
Publication date:
June 26, 2008
Applicants:
ECOLE NORMALE SUPERIEURE DE LYON, CENTRE NATIONAL DE LA RECHERCHE SCIENTRIQUE - CNRS
Abstract: The present invention relates to a bone system model comprising a mineralized matrix and osteoblasts, chararacterized in that the osteoblasts are deposited onto the matrix so as to form a layer at confluence and/or nodules, and the osteoclasts are deposited onto said layer and/or said nodules, and also to bone system models affected by various pathologies. The invention also covers the uses for carrying out screenings and tests for aggressiveness and for toxicity.
Type:
Application
Filed:
June 11, 2004
Publication date:
March 22, 2007
Applicants:
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, ECOLE NORMALE SUPERIEURE DE LYON
Inventors:
Pierre Jurdic, Olivier Destaing, Frederic Saltel, Edith Bonnelye
Abstract: A culture medium for culturing chicken embryonic stem (ES) cells is disclosed. The culture medium is used for supporting avian ES cell cultures. The components of the avian ES cell media include cytokines, fibroblast growth factors, insulin-like growth factors and stem cell growth factors and anti-retinoic acid antibody. The culture medium is substantially free of active retinoic acid. A method for culturing avian ES cells and the resulting products are also disclosed.
Type:
Grant
Filed:
May 12, 1997
Date of Patent:
September 5, 2000
Assignees:
Institute National de la Recherche Agronomique, Centre National de la Recherche Scientifique (CNRS), Ecole Normale Superieure de Lyon