Abstract: A diagnostically useful carrier includes a peptide including the amino acid sequence set forth in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 or a variant thereof. A kit, a composition, a detection method, use for detecting a neurological disease, a human autoantibody specifically binding to Drebrin and a therapeutic compound or combination for use in the treatment of a neurological use are also useful.

Abstract: Netrin G1 is disclosed as a biomarker of membranous glomerulonephritis (MGN). Netrin G1 antigen-comprising polypeptides and Netrin G1 antibodies can be used in in vitro methods and kits for diagnosis, prognosis and monitoring of MGN of patients with circulating Netrin G1 autoantibodies.

Abstract: There is proposed a method for detecting a presence of a fluorescence pattern type on an organ segment via immunofluorescence microscopy and digital image processing. The steps comprise: provision of the organ segment, incubation of the organ segment with a liquid patient sample, incubation of the organ segment with secondary antibodies which have been labelled with a fluorescent dye, acquisition of a fluorescence image of the organ segment in a colour channel corresponding to the fluorescent dye, and provision of the fluorescence image to a neural network. What takes place by means of the neural network is simultaneous determination of segmentation information through segmentation of the fluorescence image and, furthermore, of a measure of confidence indicating an actual presence of the fluorescence pattern type.

Abstract: A diagnostically useful carrier includes a means for specifically capturing an antibody to a polypeptide from the group including squid MLC1 or squid MLC2 or a variant thereof in a sample from a subject. A method includes the step detecting in a sample from a subject the presence or absence of an antibody to squid MLC1 or squid MLC2. The polypeptide or the carrier or a polypeptide binding specifically to an IgE antibody from the sample of a patient to squid MLC1 or squid MLC2 are useful for the manufacture of a diagnostic kit, preferably for the diagnosis of allergy.

Abstract: A method is used for the qualitative evaluation of real-time PCR data, where a time/PCR amplification plot of an associated sample is classified as a negative plot or as a positive plot. The method involves providing a real-time PCR amplification plot to be classified, plotting at least 20 successive amplitude values of corresponding successive PCR cycle indices of the sample. Next, a quality metric is determined, on the basis of the at least one amplitude value. A first criterion is determined by a comparison of the quality metric with a first standard value. A sequence of values is then determined, which indicates a gradient of the PCR amplification plot to be classified, and a second criterion is determined as to whether the sequence of values exceeds a second standard value. Finally, the real-time PCR amplification plot is classified as a positive plot if all the criteria given above are satisfied.

Abstract: A method and a device are useful for detecting a binding of autoantibodies from a patient sample to double-stranded deoxyribonucleic acid (DNA) using Crithidia luciliae cells by fluorescence microscopy and by digital image processing.

Abstract: A method is proposed for detecting an error state when aspirating a liquid, including: immersing a tip of an aspiration needle in the liquid, generating a negative pressure in the aspiration needle for a predefined time period to aspirate a predetermined partial volume of the liquid in the aspiration needle, continuously acquiring a sensor signal curve by means of continuous measurement of a sensor signal, which indicates a pressure in the aspiration needle, during an overall time period, which comprises the predetermined time period and furthermore a further time period following the predetermined time period, detecting the error state in the case that the sensor signal falls below a first threshold value during the predetermined time period, characterized by providing a reference signal curve, determining a deviation measure, which indicates a deviation of the sensor signal curve from the reference signal curve during the further time period, providing a predetermined second threshold value, detecting the e

Abstract: An apparatus is proposed for identifying respective cover slip regions of respective cover slips having respective tissue sections on a specimen slide, which has multiple optical identifiers. The apparatus includes a planar light source, an image acquisition unit, a holding unit for positioning the specimen slide between the planar light source and the image acquisition unit, a slit diaphragm, which has multiple opening slits, reversibly positionable between the planar light source and the specimen slide, and an illumination unit, which is designed to illuminate that surface of the specimen slide which faces toward the image acquisition unit. Furthermore, the apparatus includes a monitoring unit which is designed, on the basis of a completely illuminated transmitted light image, an incident light image, and a partially darkened transmitted light image, to assign respective tissue sections to respective optical identifiers.

Abstract: A method for detecting at least one potential presence of at least one fluorescence pattern type on an organ section by means of immunofluorescence microscopy and by means of digital image processing is disclosed. An organ section is provided on a slide and the section is incubated with a liquid patient sample which potentially includes primary antibodies and with secondary antibodies which have been labelled with a fluorescent dye. A fluorescence image of the organ section is acquired. A determination is made, by segmentation of the fluorescence image by means of a first neural network, of a sub-area of the fluorescence image that is relevant to formation of the fluorescence pattern type. A determination is made, on the basis of the fluorescence image by means of a second neural network, of the measure of confidence that indicates an actual presence of the fluorescence pattern type.

Abstract: A diagnostically useful carrier includes (a) a peptide including the amino acid sequence set forth in SEQ ID NO:1 or a variant thereof, and (b) a somatic lysate of Toxocara canis larvae. Further, a kit, use, methods, and compositions that include the diagnostically useful carrier are disclosed.

Abstract: A nucleic acid or peptide detection system that includes a carrier with at least two areas is provided. A method and kit that use and/or include the nucleic acid peptide detection system are also provided.

Abstract: A method and microscopy system are useful for recording an image of a sample region. A laser beam is directed onto the sample region with interface(s). An objective lens facilitates images the laser beam on a focusing point which lies on the optical axis of the objective lens or an axis parallel thereto, and which lies in a focusing plane. The objective lens and the sample region are displaced with respect to one another in relative fashion along the optical axis of the objective lens to different relative displacement positions. Intensity values of the laser beam are captured for a respective relative displacement position. A respective highest intensity value for a respective displacement position, a curve of the highest intensity values, and a reference relative displacement position from at least one maximum of the curve, are determined. Image(s) of the sample region is/are captured at the reference relative displacement position.

Abstract: An elongated incubation trough has an indentation open toward a top end as well as a bottom. The indentation has a first receiving area to receive an elongated test strip as well as a second receiving area to receive an end section of a fluid line. The second receiving area is in fluidic communication with the first receiving area. A maximum width of the second receiving area at bottom height is greater than a maximum width of the first receiving area at bottom height.

Abstract: A method can be used for detecting, in a sample, an autoantibody binding to a polypeptide having the sequence SEQ ID NO: 1, SEQ ID NO: 11 or SEQ ID NO: 24. A carrier containing said polypeptide and an autoantibody binding to said polypeptide are useful. The autoantibody may be used for the diagnosis of a disease, and a method can be used for isolating an autoantibody binding to said polypeptide. The polypeptide and a kit containing said polypeptide are useful, and can be used for the manufacture of a kit or medical device. A method involves contacting a medical or diagnostic device containing said polypeptide with a buffered solution containing an antibody binding specifically to said polypeptide. A sample containing said autoantibody as a positive control and a diluted sample containing said autoantibody are useful.

Abstract: A method involves detecting in a sample an autoantibody binding specifically to a mammalian VGLUT. An autoantibody binding specifically to a mammalian VGLUT and a diagnostically useful carrier with a solid phase with an immobilized polypeptide containing a mammalian VGLUT or a variant thereof, are useful. The autoantibody can be used for diagnosing a neurological autoimmune disease or a cancer.

Abstract: An incubation device has multiple rocking platforms with each platform pivotally mounted about a respective axis. Each rocking platform has a respective receiving device for mechanically reversibly receiving a respective incubation channel. The device also has a common drive unit for common respective rocking movements of each platform from a zero position through with a specific angle range as a result of movement of the drive unit. Each rocking platform is coupled to the drive unit via a respective coupling mechanism, and each coupling mechanism elastically and resiliently couples the respective rocking platform to the drive unit, such that when the rocking platform is restrained, despite continuation of the drive movement, the rocking platform remains motionless in a fixed position. After release of the restraint on the rocking platform, the rocking platform resumes the rocking movement with the specific angle range.

Abstract: A method and apparatus are provided for detecting respective potential presences of respective different cellular fluorescence pattern types on a biological cellular substrate including human epithelioma cells (HEp cells), wherein the fluorescence pattern types include different antinuclear antibody fluorescence pattern types. A method is also provided for detecting potential presences of different cellular fluorescence pattern types on a biological cellular substrate including human epithelioma cells by means of digital image processing, as well as a computing unit, a data network device, a computer program product and a data carrier signal therefor.

Abstract: A primer pair that includes a forward primer and a reverse primer is used to amplify a nucleic acid from a pathogen associated with a skin, hair and nail infection that includes SEQ ID NO: 22. A nucleic acid capable of hybridizing specifically to a nucleic acid sequence from a pathogen associated with a skin, hair and nail infection that includes SEQ ID NO: 22 is provided. A carrier that includes the nucleic acid is provided. A method can be used to detect in a sample a nucleic acid sequence including SEQ ID NO: 22 from a pathogen associated with a skin, hair, and nail infection. The primer pair, the nucleic acid, or the carrier may be useful for the diagnosis of a disease. A kit including the primer pair, the nucleic acid, and/or the carrier may be useful for the diagnosis of a disease.

Abstract: The present invention relates to a method for diagnosing a disease comprising the step detecting in a sample comprising antibodies from a patient an autoantibody binding to a polypeptide selected from the group comprising NSF, STX1B, DNM1 and VAMP2, a polypeptide comprising a polypeptide selected from the group comprising NSF, STX1B, DNM1 and VAMP2, or a variant thereof, a use of said polypeptide for the diagnosis of a disease, an autoantibody binding to a polypeptide selected from the group comprising NSF, STX1B, DNM1 and VAMP2, a use of the autoantibody for the diagnosis of a disease, a method for isolating an autoantibody binding to a polypeptide selected from the group comprising NSF, STX1B, DNM1 and VAMP2, a pharmaceutical composition or medical device comprising said polypeptide according to the present invention, a kit for the diagnosis of a disease comprising said polypeptide or said medical device and a use of said polypeptide or autoantibody for the manufacture of a kit or medical device.