Abstract: A method is used for diagnosing a disease by detecting in a sample with antibodies from a patient an autoantibody binding to DAGLA.

Abstract: A diagnostically useful carrier includes a peptide including the amino acid sequence set forth in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 or a variant thereof. A kit, a composition, a detection method, use for detecting a neurological disease, a human autoantibody specifically binding to Drebrin and a therapeutic compound or combination for use in the treatment of a neurological use are also useful.

Abstract: The invention relates to a device and a method for automated evaluation of incubated immunoblot strips (5). What is essential for such immunoblot strips (5) is that these have regions coated with antigens, which regions discolour under the formation of so-called bands (6) as soon as a binding occurs, in particular between the antigen and an antibody present in a patient sample. Within the scope of the described technical solution, a camera (2) is initially used to make recordings of the surface of an incubated immunoblot strip (5) and these recordings are transmitted in digitized form to an evaluation unit (1). Finally, the discolourings, which occurred in the form of bands (6), are detected and at least partly quantified in the evaluation unit (1), such that a diagnosis suggestion is generated taking into account the number of bands (6) and the colour strength thereof.

Abstract: A recombinant polypeptide can be used in the diagnosis of the presence of a Zika virus in a patient. The recombinant polypeptide includes SEQ ID NO: 1 or a variant thereof. The recombinant peptide may be a monomer, a dimer, or a hexamer.

Abstract: A method is for detecting respective potential presence of respective different antinuclear antibody fluorescence pattern types on a biological cell substrate including human epithelioma cells, including: acquiring a first image which represents staining of the cell substrate by a first fluorescent dye, and acquiring a second image which represents staining of the cell substrate by a second fluorescent dye, detecting, on the basis of the first image, respective image segments which in each case represent at least one mitotic cell, selecting, on the basis of the detected image segments, subimages of the first image and subimages corresponding thereto of the second image and detecting, on the basis of the selected subimages of the first image and of the selected subimages of the second image, respective actual presence of respective cellular fluorescence pattern types by means of a convolutional neural network.

Abstract: A diagnostically useful carrier includes (a) a peptide including the amino acid sequence set forth in SEQ ID NO:1 or a variant thereof, and (b) a somatic lysate of Toxocara canis larvae. Further, a kit, use, methods, and compositions that include the diagnostically useful carrier are disclosed.

Abstract: A sample support has an absorbent membrane layer which is based on a nonwoven polyolefin and which has at least one take-up region with dried blood constituents. It moreover has a film layer based on a plastic, where the film layer has received antistatic pretreatment. The membrane layer and the film layer have been connected to one another mechanically in one subregion of the sample support. but the layers have moreover not been connected to one another mechanically in the take-up region. It is preferable that, when the sample support is placed onto a uniformly level surface the membrane layer covers the film layer at least in the take-up region.

Abstract: A diagnostically useful carrier includes a means for specifically capturing an antibody to a polypeptide from the group including squid MLC1 or squid MLC2 or a variant thereof in a sample from a subject. A method includes the step detecting in a sample from a subject the presence or absence of an antibody to squid MLC1 or squid MLC2. The polypeptide or the carrier or a polypeptide binding specifically to an IgE antibody from the sample of a patient to squid MLC1 or squid MLC2 are useful for the manufacture of a diagnostic kit, preferably for the diagnosis of allergy.

Abstract: A diagnostically useful carrier has a polypeptide for specifically capturing an antibody to Ara h 7 isotype 7.0201 in a sample from a subject. A method includes detecting in a sample from a subject the presence or absence of an antibody to Ara h 7 isotype 7.0201. A pharmaceutical composition includes Ara h 7 isotype 7.0201 or a variant thereof.

Abstract: A recombinant or chemically synthesized polypeptide includes SEQ ID NO: 1 or a variant thereof. A diagnostically useful carrier includes means for specifically capturing an antibody to SEQ ID NO: 1 in a sample from a subject. A kit includes the polypeptide or the diagnostically useful carrier. A method for diagnosing, proposing, or monitoring the treatment of a virus, preferably Flavivirus, more preferably POWV infection includes detecting in the sample from a subject the presence or absence of an antibody to SEQ ID NO: 1 and/or an antibody to SEQ ID NO: 2. A polypeptide including SEQ NO: 1 and/or SEQ ID NO: 2, and/or a variant thereof is useful for the diagnosis. An IgIVI antibody to SEQ ID NO: 1 or means for specifically capturing an IgM class antibody to SEQ ID NO: 1 is useful for increasing the diagnostic reliability of an immunoassay for the diagnosis of a virus infection.

Abstract: A method is used for optical scanning of at least one object region placed on a transparent specimen holder. The method is as follows: for each sample lateral position of plural predefined sample lateral positions performing a focus determination by: performing laser reflection and using a first camera taking plural first images to determine a reference distance between the specimen holder and an objective lens; performing transmission flash illumination and using a second camera taking plural second images to define a focus distance taking into account the reference distance; after completing the focus determination, determining a focus distance topology across the object region based on the focus distances determined for ail sample lateral positions; and laterally moving the specimen holder and acquiring third images while focusing according to the focus distance topology.

Abstract: A method is used for acquiring and displaying an immunofluorescence image of a biological sample. During a first operating state, the sample is continuously illuminated using the excitation radiation. A relative position between the sample and an optical system, which guides the fluorescent radiation to an image sensor, is changed in dependence on a movement gesture of a user, and a corresponding digital image is displayed. Upon detection of a termination of the movement gesture, a change is made from the first operating state to a second operating state. In the second operating state, the sample is firstly illuminated using the excitation radiation to excite the fluorescent radiation of the fluorescent pigment. An acquisition of fluorescent radiation emitted by the sample then takes place and a corresponding, further digital image is generated.

Abstract: Provided is a polypeptide comprising a macadamia protein running in a 2D SDS PAGE at pH 6.3 to 8.7 and 53 to 67 kDa or a protein running in a 2D SDS PAGE at pH 6.5 to 7.9 and 20 to 25 kDa or an antigenic variant thereof, and a tag for detection and/or purification, a polypeptide fused with the macadamia protein, or the macadamia protein is modified by glycosylation, phosphorylation, acetylation, decarboxylation, citrullination, or hydroxylation.

Abstract: A recombinant polypeptide can be used in the diagnosis of the presence of a Zika virus in a patient. The recombinant polypeptide includes SEQ ID NO1 or a variant thereof, where the recombinant polypeptide is a monomer, a dimer, or a hexamer.

Abstract: A carrier includes at least one take-up region based on a non-woven polyolefin containing dried-up capillary blood. The carrier is useful for semi-quantitatively, preferably quantitatively determining the concentration of an analyte in a blood sample. Further, a method is used for detecting an analyte in a blood sample and includes the steps of providing the carrier, applying a blood sample to the take-up region of the carrier, drying the carrier, contacting the carrier containing the dried-up capillary blood with a liquid which is suitable for taking up the analyte from the dried-up blood sample, and detecting the analyte in the liquid.

Abstract: A method for diagnosing a limbic encephalitis, PNS, encephaloymyelitis, leukoencephalopathy, retinitis or optic atrophy, includes detecting a bornavirus in a sample, preferably from the group comprising mammalian 2 bornavirus, even more preferably VSBV-1, and mammalian 1 bornavirus, even more preferably BoDV-1; to a carrier containing a means for the capture of an antibody against at least one polypeptide selected from the group of BoDV-1-N, BoDV-1-P, VSBV-N and VSBV-P. A kit contains the carrier and a means for the detection of a captured antibody and a control for the detection of the presence of a sample. The carrier or kit or of a primer can be used for the detection of a bornavirus or a probe can be used for the diagnosis of an encephalitis, for the prognostication of post-transplantation complications, for the monitoring of a therapy of an encephalitis or of post-transplantation complications or for the differentiation between an autoimmune encephalitis and an encephalitis caused by infection.

Abstract: A method and a device are useful for detecting a binding of autoantibodies from a patient sample to double-stranded deoxyribonucleic acid (DNA) using Crithidia luciliae cells by fluorescence microscopy and by digital image processing.