Patents Assigned to Evolva SA
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Patent number: 10119154Abstract: The present disclosure provides methods for identifying anthocyanins with improved stability, color, or hue using a screening method, and producing anthocyanins with improved stability, color, or hue in, for example, host cells comprising one or more heterologous glycosyltransferase nucleic acid molecules and one or more heterologous acyltransferase nucleic acid molecules.Type: GrantFiled: December 23, 2013Date of Patent: November 6, 2018Assignee: Evolva SAInventors: Jorgen Hansen, Patricia Hoyle, Michael Naesby, Joseph M. Sheridan, Jonathan R. Heal
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Publication number: 20180242591Abstract: Insect repellent and pesticide compositions including a santalene component are disclosed.Type: ApplicationFiled: August 23, 2016Publication date: August 30, 2018Applicant: Evolva SAInventors: Jean Davin Amick, Deepali Saran
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Patent number: 10053717Abstract: The invention described materials and methods for producing oxidized 13R-MO, such as forskolin. In particular, the invention describes P450s involved in oxidation of 13R-MO including CYP76AH8, CYP76AH11, CYP76AH15, CYP76AH17, CYP71D381 and CYP76AH9. Host organisms expressing one or more of these P450s are useful in the production of oxidized 13R-MO.Type: GrantFiled: January 30, 2015Date of Patent: August 21, 2018Assignees: University of Copenhagen, Danmarks Tekniske Universitet, Evolva SAInventors: Björn Hamberger, Birger Lindberg Møller, Eirini Pateraki, Johan Andersen-Ranberg, Niels Bjerg Jensen
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Patent number: 10017804Abstract: Recombinant microorganisms are disclosed that produce steviol glycosides and have altered expression of one or more endogenous transporter or transcription factor genes, or that overexpress one or more heterologous transporters, leading to increased excretion of steviol glycosides of interest.Type: GrantFiled: February 11, 2014Date of Patent: July 10, 2018Assignee: EVOLVA SAInventors: Ernesto Simon, Iben Nordmark Anderson, Michael Dalgaard Mikkelsen, Jorgen Hansen, Veronique Douchin
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Patent number: 10011859Abstract: The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.Type: GrantFiled: October 1, 2014Date of Patent: July 3, 2018Assignee: EVOLVA SAInventors: Yaoquan Liu, Jorgen Hansen, Jens Houghton-Larsen, Muthuswamy Panchapagesa Murali, Sathish Kumar, Nina Nicoline Rasmussen
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Publication number: 20180179562Abstract: A method for producing an objective substance such as phytosphingosine and sphinganine using yeast is provided. An objective substance is produced by cultivating yeast having an ability to produce the objective substance and modified so that the expression and/or activities of proteins encoded by LCB4 and CKA2 genes are reduced in a culture medium, and collecting the objective substance from cells of the yeast and/or the culture medium.Type: ApplicationFiled: February 16, 2018Publication date: June 28, 2018Applicants: AJINOMOTO CO., INC., EVOLVA SAInventors: Markus Schwab, Maud Babau, David Fischer, Anaelle Hatsch, Sabina de Andrade Pereira Tavares, Curt Aime Friis Nielsen, Jens Klein, Corina Daniela Wirdnam
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Publication number: 20180179563Abstract: A method for producing an objective substance such as sphingoid bases and sphingolipids using yeast is provided. An objective substance is produced by cultivating yeast having an ability to produce the objective substance in a culture medium containing an additive that is able to associate with, bind to, solubilize, and/or capture the objective substance, and collecting the objective substance from cells of the yeast and/or the culture medium.Type: ApplicationFiled: February 16, 2018Publication date: June 28, 2018Applicants: AJINOMOTO CO., INC., EVOLVA SAInventors: Markus Schwab, Corina Daniela Wirdnam, Thomas Oestergaard Tange, Maud Babau, Anaelle Hatsch, Fanny Delegrange, David Fischer, Sabina de Andrade Pereira Tavares
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Patent number: 9957540Abstract: Methods for recombinant production of steviol glycoside and compositions containing steviol glycosides are provided herein.Type: GrantFiled: February 6, 2014Date of Patent: May 1, 2018Assignee: EVOLVA SAInventors: Michael Dalgaard Mikkelsen, Jorgen Hansen, Ernesto Simon, Federico Brianza, Angelika Semmler, Kim Olsson, Simon Carlsen, Louis Düring, Alexei Ouspenski, Paula Hicks
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Patent number: 9932619Abstract: The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.Type: GrantFiled: December 4, 2013Date of Patent: April 3, 2018Assignee: EVOLVA SAInventors: Yaoquan Liu, Jorgen Hansen, Jens Houghton-Larsen, Muthuswamy Panchapagesa Murali, Sathish Kumar, Nina Nicoline Rasmussen
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Patent number: 9920349Abstract: Methods and materials for enzymatic synthesis of mogroside compounds described.Type: GrantFiled: November 19, 2012Date of Patent: March 20, 2018Assignee: Evolva SAInventors: Yaoquan Liu, Jung Yeop Lee, Monika Khare
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Patent number: 9834793Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.Type: GrantFiled: April 20, 2015Date of Patent: December 5, 2017Assignee: EVOLVA SAInventors: Michael Katz, Hans Peter Smits, Jochen Forster, Jens Bredal Nielsen
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Patent number: 9834800Abstract: This invention provides recombinant cells and methods for producing terpenes and terpenoids by increasing production or accumulation or both of isoprenoid precursors thereof.Type: GrantFiled: August 19, 2013Date of Patent: December 5, 2017Assignee: Evolva SAInventors: Thomas Oestergaard Tange, Michael Naesby, Christophe Folly, Fanny Delegrange, Jens Houghton-Larsen, Simon Carlsen
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Patent number: 9828617Abstract: Microorganisms and processes for the recombinant manufacture of clavine-type alkaloids such as cycloclavine, festuclavine, agroclavine, chanoclavine and chanoclavine aldehyde, as well as polypeptides, polynucleotides and vectors comprising such polynucleotides which can be applied in a method for the manufacture of clavine-type alkaloids are provided.Type: GrantFiled: August 13, 2013Date of Patent: November 28, 2017Assignees: BASF SE, Evolva SAInventors: Michael Naesby, Christophe Folly, Curt Aimé Friis Nielsen, Anaelle Hatsch, Markus Schwab, Oskar Zelder, Stefan Haefner, Hartwig Schroeder, Birgit Hoff, Andrea Molt, Klaus Ditrich, Michael Breuer, Holger Hartmann, Karsten Koerber
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Patent number: 9822374Abstract: A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.Type: GrantFiled: May 24, 2011Date of Patent: November 21, 2017Assignee: EVOLVA SAInventors: Michael Patrik Katz, Thomas Durhuus, Hans Peter Smits, Jochen Förster
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Publication number: 20170314049Abstract: The invention relates to recombinant microorganisms and methods for producing manoyl oxide.Type: ApplicationFiled: November 13, 2015Publication date: November 2, 2017Applicant: Evolva SAInventor: Niels Bjerg Jensen
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Patent number: 9725743Abstract: A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4 -hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.Type: GrantFiled: January 2, 2013Date of Patent: August 8, 2017Assignee: Evolva SAInventors: Michael Katz, Jochen Forster, Helga David, Hans Peter Schmidt, Malin Sendelius, Sara Peterson Bjorn, Thomas Thomasen Durhuus
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Patent number: 9631215Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.Type: GrantFiled: August 8, 2012Date of Patent: April 25, 2017Assignee: EVOLVA SAInventors: Jens Houghton-Larsen, Paula Hicks, Michael Naesby, Thomas Tange Ostergaard, Jorgen Hansen, Michael Mikkelsen Dalgaard, Esben Hansen Halkjaer, Ernesto Simon, Sabina De Andrade Periera Tavares
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Patent number: 9562251Abstract: Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.Type: GrantFiled: June 2, 2011Date of Patent: February 7, 2017Assignee: Evolva SAInventors: Ganesh M. Kishore, Jorgen Hansen, Jens Houghton-Larsen, Esben Halkjær Hansen, Michael Dalgaard Mikkelsen, Sabina Tavares, Charlotte Blom
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Patent number: 9476082Abstract: Yeast strains capable of increased prenyl phosphate production are provided, enabling increased terpenoid molecule production. Heterologous yeast strains with high prenyl phosphate availability are prepared using one or both of two different strategies for increasing the availability of prenyl phosphates for terpenoid production. First, by co-expressing multiple mevalonate pathway gene analogs, a novel heterologous combination of genes results, some of which increases the inherent availability of prenyl phosphates in yeast. Second, by expressing the non-endogenous enzyme ATP citrate lysase (ACL), a buildup of high cytosolic concentration of acetyl-CoA is produced in the cytosol of S. cerevisiae.Type: GrantFiled: May 20, 2011Date of Patent: October 25, 2016Assignee: Evolva SAInventor: Jorgen Hansen
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Patent number: 9404129Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.Type: GrantFiled: October 15, 2014Date of Patent: August 2, 2016Assignee: Evolva SAInventors: Michael Katz, Hans Peter Smits, Jochen Forster, Jens Bredel Nielsen