Patents Assigned to Gist-Brocades B.V.
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Patent number: 6867015Abstract: The present invention relates to a microbioligcal test method for detecting antibacterial compounds in liquid samples such as milk, meat juice, serum and urine. The method employs an acid-base or redox indicator to detect growth of a test organism and is characterised in that a thermophilic strain of Bacillus or Streptococcus, e.g. Bacillus stearothermophilus var. calidolactis C953, is employed at a concentration of above 107 CFU/ml, preferably between 2×107 and 3×108 CFU/ml.Type: GrantFiled: February 1, 1996Date of Patent: March 15, 2005Assignee: Gist-Brocades B.V.Inventors: Pieter Cornelis Langeveld, Robert Beukers, Michiel Wilhelmus Christianus Bommele, Jacobus Stark
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Patent number: 6656513Abstract: The present invention discloses a bread improving composition containing cellobiohydrolase and its use in bread making.Type: GrantFiled: May 25, 2001Date of Patent: December 2, 2003Assignee: Gist Brocades B. V.Inventor: Rudolf Franciscus Wilhelmus Cornelus Van Beckhoven
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Patent number: 6503727Abstract: The invention relates to a process for the preparation of an antibiotic, in particular cefalexin, ampicillin, amoxicillin, cefaclor, cefradin, cefadroxil, cefotaxim and the like, wherein a beta-lactam core is acylated, the antibiotic is recovered from the reaction mixture, the remaining mother liquor is subjected to a hydrolysis reaction in which the antibiotic present in the mother liquor is decomposed into its initial compounds, in particular the beta-lactam core, and the acylation agent is hydrolized. The beta-lactam core can then be recovered virtually quantitatively or recycled to the acylation reaction. This is because it has been found that the solubility of the beta-lactam core is unexpectedly high at relatively high concentrations of acylation agent and antibiotic.Type: GrantFiled: June 5, 1998Date of Patent: January 7, 2003Assignee: Gist-Brocades, B.V.Inventors: Wilhelmus H. J. Boesten, Wilhelmus J. J. Van Den Tweel, Rocus M. Dekkers
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Patent number: 6306635Abstract: The present invention provides polypeptides with cellulase activity, corresponding DNA sequences, vectors, transformed hosts and a method for the production of these polypeptides, which are obtainable from Aspergillus niger var. niger or Aspergillus niger var. tubigensis.Type: GrantFiled: January 7, 2000Date of Patent: October 23, 2001Assignee: Gist-Brocades B.V.Inventors: Henriëtte C. Van Den Broeck, Leendert H. De Graaff, Jacob Visser, Albert J. J. Van Ooijen
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Patent number: 6255505Abstract: The present invention discloses a microbial polyunsaturated fatty acid(PUFA)-containing oil with a high triglyceride content and a high oxidative stability. In addition, a method is described for the recovery of such oil from a microbial biomass derived from a pasteurised fermentation broth, wherein the microbial biomass is subjected to extrusion to form granular particles, dried and the oil then extracted from the dried granules using an appropriate solvent.Type: GrantFiled: March 19, 1997Date of Patent: July 3, 2001Assignee: Gist-Brocades, B.V.Inventors: Hendrik Louis Bijl, Johannes Hendrik Wolf, Albert Schaap, Johannes Martinus Jacobus Visser
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Patent number: 6242006Abstract: &bgr;-Lactam granules free of organic solvents have been provided for. Also a process to prepare the same by applying during the granulation only water as binding solvent has been disclosed.Type: GrantFiled: December 24, 1998Date of Patent: June 5, 2001Assignee: Gist-Brocades B.V.Inventors: Gerard Jan Kwant, Nicolaas Henricus Scheffers
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Patent number: 6190890Abstract: The present invention relates to peptides with multiple enzymatic activitives: carboxymethyl cellusase(CMCase), endoglucanase and &bgr;-glucanase, corresponding nucleotides, vectors and transformed hosts, methods of preparation and use. The peptide is used in the food industry for the liquefaction of plant cell material, e.g., in vegetable or fruit juice manufacture, or as processing aids to reduce the fouling of membranes.Type: GrantFiled: June 12, 1997Date of Patent: February 20, 2001Assignee: Gist-brocades, B.V.Inventors: Henriëtte C. Van Den Broeck, Leendert H. De Graaff, Jacob Visser, Albert J. J. Van Ooijen
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Patent number: 6190709Abstract: A flavor enhancer which is low in monosodium glutamate, which is substantially free of 5′-IMP and 5′-GMP and which enhances both meat, vegetable and diary flavors. The use of the flavor enhancer in flavoring compositions and food and feed applications.Type: GrantFiled: August 20, 1999Date of Patent: February 20, 2001Assignee: Gist-Brocades B.V.Inventors: Arie Cornelis Schoenmaker, Johanna Plijter-Schuddemat, Luppo Edens
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Patent number: 6187761Abstract: The present invention provides a process for the preparation of a composition comprising natural vitamin B12, wherein said process comprises the steps of: a) obtaining microbial cells containing natural vitamin B12, b) causing opening of the microbial cells such that at least part of the soluble content of the cells comprising vitamin B12 is released in a liquid in which the cells are contained, c) separating the opened cells and the liquid comprising the vitamin B12, d) preparing a mixture of the vitamin B12 and at least a part of the opened cells, wherein the mixture has a vitamin B12 concentration on dry matter in excess of 0.1% (w/w).Type: GrantFiled: January 19, 1999Date of Patent: February 13, 2001Assignee: Gist-Brocades B.V.Inventor: Hendrik Louis Bijl
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Patent number: 6166230Abstract: The present invention relates to a process of treating an oil, the process comprising contacting the oil with a polar solvent to extract at least one compound that is soluble in the solvent, and then separating the solvent containing the compound from the so treated oil. The oil is microbially derived, and extracted either from a fermentation broth or a filtrate thereof using hexane. The compound to be extracted is usually a sterol or a diglyceride. The solvent is ethanol having up to 5% water. The oil can contain a polyunsaturated fatty acid such as C18, C20 or C22 .omega.-3 or .omega.-6 fatty acid, such as arachidonic acid.Type: GrantFiled: February 8, 1999Date of Patent: December 26, 2000Assignee: Gist-brocades B.V.Inventors: Hendrik Louis Bijl, Johannes Hendrik Wolf, Albert Schaap
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Patent number: 6159725Abstract: Transformed yeasts are provided which constitutively express a hexose transporter gene. The transformed yeast can be used in the beer, bread, wine and whisky production.Type: GrantFiled: January 16, 1997Date of Patent: December 12, 2000Assignee: Gist-brocades, B.V.Inventors: Paul Klaassen, Rutger Jan Van Rooijen
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Patent number: 6150143Abstract: A process for the recovery of natamycin from a fermentation broth containing biomass and natamycin, which comprises:(a) disintegrating the biomass; and(b) separating the natamycin from the thus treated fermentation broth.Type: GrantFiled: February 7, 1997Date of Patent: November 21, 2000Assignee: Gist-Brocades B.V.Inventors: Dilipkoemar Raghoenath, Josephus J. P. Webbers
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Patent number: 6083733Abstract: The present invention discloses enzymes having xylanase activity. The xylanases are characterized in that they are active at a temperature of 80.degree. C. or higher. The enzymes are obtainable from anaerobic thermophilic bacteria. The enzymes are suited for use in paper and pulp production processes. The invention also describes cloning and expression of genes having xylanase activity obtained from the deposited strains.Type: GrantFiled: February 5, 1997Date of Patent: July 4, 2000Assignee: Gist-brocades B.V.Inventors: Vidar Gronberg, Simon Forster, Dean Moody, Diane P. Williams, Sara Iverson, Roberta Lee Farrell, Peter Leonard Bergquist, McIver Daniel, Hugh William Morgan, Wilhelmus Johannes Quax, Margareta Adriana Herweijer, Brian Edward Jones
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Patent number: 6071713Abstract: A bioprocess for preparing adipoyl-7-ACA comprising the steps: (a) transforming cells of a strain of Penicillium chrysogenum which produces isopenicillin N with an expression vector containing DNA encoding an enzyme, having expandase activity capable of accepting adipoyl 6-APA as a substrate, an enzyme having hydroxylase activity capable of accepting adipoyl-7-ADCA as a substrate and an enzyme having acetyl transferase activity capable of accepting adipoyl 7-ADAC as a substrate; (b) culturing the transformed cells from step a) in a suitable culture medium containing an adipate feedstock, wherein said cells produce adipoyl 6-APA; and (c) culturing the transformed cells producing adipoyl 6-APA of step b) under conditions suitable for expression of said DNA encoding enzyme, thereby producing the end product adipoyl-7-ACA.Type: GrantFiled: June 28, 1999Date of Patent: June 6, 2000Assignee: Gist-Brocades B.V.Inventors: Michael J. Conder, John A. Rambosek, Phyllis C. McAda, Christopher D. Reeves
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Patent number: 6066356Abstract: An arabinoxylan-degrading enzyme from Aspergillus is provided. The enzyme is active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.Type: GrantFiled: October 13, 1998Date of Patent: May 23, 2000Assignee: Gist brocades B.V.Inventors: Monique Josina Andrea Van Der Wouw, Albert Johannes Joseph Van Ooijen, Marcus Matheus Catharina Gielkens, Leendert Hendrik De Graaff, Jacob Visser
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Patent number: 6060268Abstract: Penicillin G acylase is immobilized by covalent bonding to a crosslinked mixture of a gelled gelling agent such as gelatin and a polymer containing free amino groups such as alginate amine, chitosan or polyethylene imine. The immobilized penicillin G acylase provides a higher synthesis/hydrolysis ratio as compared to immobilizing with other carriers when producing .beta.-lactam derivatives by a condensing reaction of an amino .beta.-lactam with an acylating agent. The acylating agent may be a derivative of D-phenylglycine, a derivative of D-p-hydroxyphenylglycine or a derivative of D-2,5-dihydro-phenylglycine. Examples of .beta.-lactam derivatives that can be produced are amoxycillin, ampicillin, cephaclor, cephadroxil, cephprozil, cephalexin and cephradine.Type: GrantFiled: January 15, 1998Date of Patent: May 9, 2000Assignee: Gist-Brocades B.V.Inventor: Erik De Vroom
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Patent number: 6048708Abstract: An improvement for enzymatically syhnthesizing a .beta.-lactam compound is presented. The improvement comprises catalyzing the acylation of an amino .beta.-lactam with an acylating agent for at least 5 hours with an amidase or acylase, wherein the concentration of each reactant is constantly higher than 70% of the lowest value of the saturated concentration of both reactants.Type: GrantFiled: February 19, 1997Date of Patent: April 11, 2000Assignee: Gist-Brocades B.V.Inventors: Kim Clausen, Rocus M. Dekkers
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Patent number: 6017530Abstract: The present invention discloses a process for improving the efficiency of feed utilization and/or for promoting the growth of animals in which an animal is fed a diet which comprises a composition comprising feed substance and a ready for use phospholipase additive. Preferably said composition also comprises at least one phospholipid. Said compositions are used to improve fat digestibility and to promote growth of the animal. The phospholipid is preferably lecithin and the preferred phospholipase is a mammalian phospholipase A2. In a preferred embodiment the phospholipase is produced using recombinant DNA technology to express the enzyme in a suitable host such as a microorganism or a transgenic plant.Type: GrantFiled: May 15, 1996Date of Patent: January 25, 2000Assignee: Gist-brocades, B.V.Inventors: Robert Franciscus Beudeker, Arie Karst Kies
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Patent number: 6017726Abstract: A process for making 7-aminodeacetyl-cephalosporanic acid (7-ADAC).Type: GrantFiled: April 17, 1997Date of Patent: January 25, 2000Assignee: Gist-Brocades B.V.Inventors: Michael J. Conder, John A. Rambosek, Phyllis C. McAda, Christopher D. Reeves
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Patent number: 6013255Abstract: The present invention describes stable water-in-oil emulsions comprising the following components: water, oil, a labile compound of interest, a stabilizing agent and an emulsifier. The labile compound present in the water phase is stabilized by addition of a stabilizing agent which preferably is a polyol. Specific emulsifiers are described which give rise to stable emulsions in the presence of high amounts of a polyol.Type: GrantFiled: November 26, 1997Date of Patent: January 11, 2000Assignee: Gist-brocades B.V.Inventors: Luppo Edens, Dirk Meijer, Petrus Andreas Van Paridon