Patents Assigned to Gist-Brocades
  • Antifungal composition
    Patent number: 5962510
    Abstract: An aqueous composition is disclosed which comprises 0.1 to 20 g/l of polyene fungicide, 0.5 to 50 g/l of a suitable thickener and optionally 20 to 250 g/l of salt.
    Type: Grant
    Filed: March 18, 1998
    Date of Patent: October 5, 1999
    Assignee: Gist-brocades B.V.
    Inventors: Ben Rudolf De Haan, Jacobus Stark, Vincenzo Bozzetti
  • Penicillin glycolate
    Patent number: 5955458
    Abstract: New solvates of penicillin, in particular amoxycillin glycolate has been provided for together with a simple one-step process to prepare the same.
    Type: Grant
    Filed: March 26, 1998
    Date of Patent: September 21, 1999
    Assignee: Gist-Brocades, B.V.
    Inventor: Jagdish C. Kapur
  • Production and use of compositions comprising high concentrations of vitamin B12 activity
    Patent number: 5955321
    Abstract: The present invention provides a process for the preparation of a composition comprising natural vitamin B12, wherein said process comprises the steps of:a) obtaining microbial cells containing natural vitamin B12,b) causing opening of the microbial cells such that at least part of the soluble content of the cells comprising vitamin B12 is released in a liquid in which the cells are contained,c) separating the opened cells and the liquid comprising the vitamin B12,d) preparing a mixture of the vitamin B12 and at least a part of the opened cells, wherein the mixture has a vitamin B12 concentration on dry matter in excess of 0.1% (w/w).
    Type: Grant
    Filed: August 12, 1997
    Date of Patent: September 21, 1999
    Assignee: Gist-Brocades
    Inventor: Hendrik Louis Bijl
  • Process of producing B-lactam antibiotics applying microorganisms with increased ligase activity
    Patent number: 5942411
    Abstract: The present invention relates to the biosynthesis of .beta.-lactam antibiotics. More specifically, the invention relates to processes of producing .beta.-lactam antibiotics in vivo and in vitro. Also contemplated is a novel enzyme capable of catalyzing certain steps involved in .beta.-lactam biosynthesis. Further, the invention relates to a DNA construct encoding the novel enzyme, a recombinant vector or transformation vehicle comprising the DNA construct, and finally a cell comprising the DNA construct or recombinant vector.
    Type: Grant
    Filed: June 19, 1997
    Date of Patent: August 24, 1999
    Assignee: Gist-Brocades
    Inventors: Svend Kaasgaard, Klaus N. Kristiansen, Henrik M.o slashed.lgaard
  • Biscuit doughs and biscuits products and methods of producing same
    Patent number: 5942262
    Abstract: A dough suitable for baking biscuits is presented which comprises a proteinase which is at least partially inactivated by oxidation with an oxidizing agent and a second enzyme which produces the oxidizing agent. The protease is present in 10.sup.6 to 10.sup.8 NF/kg flour, and the enzyme which produces the oxidizing agent is present in 100 to 2000 SU/kg flour. The resulting dough has a consistency between bread dough and a fully hydrolyzed dough and is used to bake biscuits.
    Type: Grant
    Filed: March 19, 1997
    Date of Patent: August 24, 1999
    Assignee: Gist-Brocades
    Inventors: Jerome Souppe, Thierry Jean-Bernard Naeye
  • Pectinesterase in the treatment of fruit and vegetables
    Patent number: 5939110
    Abstract: The present invention discusses the use of pectinesterase to demethoxylate high-methoxylated pectins. The pectinesterase makes possible the preparation of food containing fruit or vegetables in a more efficient way. Specifically, fruit and vegetable jams, jellies, compotes and soups are prepared without or with greatly reduced sugar and pectin addition. The present invention also discusses a process for producing apple sauce comprising the use of pectinesterase.
    Type: Grant
    Filed: November 6, 1996
    Date of Patent: August 17, 1999
    Assignee: Gist Brocades, N.V.
    Inventors: Catherine Marie Therese Grassin, Pierre Clement Louis Fauquembergue
  • Mutated .beta.-lactam acylase genes
    Patent number: 5935831
    Abstract: New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.
    Type: Grant
    Filed: October 10, 1995
    Date of Patent: August 10, 1999
    Assignee: Gist-Brocades, N.V.
    Inventors: Wilhelmus Johannes Quax, Onno Misset, Jan Metske Van Der Laan, Herman B. M. Lenting
  • Direct selection of transformants on acetate-containing media
    Patent number: 5925515
    Abstract: A transformant selection system has been developed, particularly for a .beta.-lactam producing strain, more particularly for P. chrysogenum, by the complementation of a mutation of said .beta.-lactam producing strain by a homologous selection marker without interfering with .beta.-lactam biosynthesis. Particularly, in applying said transformant selection system a positive selection agent, for instance fluoroacetate is used for the isolation of fac mutants of said strain, particularly of said .beta.-lactam producing strain. Furthermore, a gene entitled facA has been isolated from P. chrysogenum.
    Type: Grant
    Filed: June 11, 1992
    Date of Patent: July 20, 1999
    Assignee: Gist-brocades B.V.
    Inventors: Willem Van Hartingsveldt, Robert Franciscus Maria Van Gorcom, R. J. Gouka, Roelof Ary Lans Bovenberg
  • De-esterification process
    Patent number: 5922861
    Abstract: An efficient process for de-esterification has been provided for by application of special tetrahalogenides. By applying this process a new compound, viz. cefesone, and especially the E-isomer thereof, has been prepared.
    Type: Grant
    Filed: April 1, 1998
    Date of Patent: July 13, 1999
    Assignee: Gist-Brocades B.V.
    Inventors: Erik De Vroom, Thomas Van Der Does
  • Phytosphingosine-based ceramide I analogs
    Patent number: 5919960
    Abstract: The present invention describes phytospringosine-based analogs of ceramides I, as well as cosmetics and pharmaceutical compositions containing these novel compounds and their use.
    Type: Grant
    Filed: April 29, 1996
    Date of Patent: July 6, 1999
    Assignee: Gist-brocades, B.V.
    Inventors: Pieter Gijsbert Weber, Johannes Wilhelmus Jacobus Lambers, Hein Simon Koger, Jan Verweij
  • Process for increasing the volume of a baked product
    Patent number: 5916607
    Abstract: The present invention relates to use of cyclodextrin glucanotransferase in the preparation of doughs for baked products, e.g. bread doughs. In particular, the invention provides a process for the preparation of a dough for a baked product which includes incorporating into the dough cyclodextrin glucanotransferase in an amount to increase the volume of the baked product.
    Type: Grant
    Filed: May 27, 1997
    Date of Patent: June 29, 1999
    Assignee: Gist-brocades B.V.
    Inventors: Johanna Henrica Gerdina Maria Mutsaers, Johannes Henricus Van Eijk
  • Baker's yeast and a method producing it
    Patent number: 5916609
    Abstract: A process is disclosed for the production of baker's yeast which comprises the fermentation of a baker's yeast strain using a non-molasses carbon source until a fermentation broth having at least 10%, preferably at least 13%, more preferably at least 16% of dry solids content is formed, which can be used directly as a cream yeast without concentration.
    Type: Grant
    Filed: July 28, 1997
    Date of Patent: June 29, 1999
    Assignee: Gist-brocades, B.V.
    Inventor: Pieter Jan Arnoldus Maria Plomp
  • Production of aminopeptidases form aspergillus niger
    Patent number: 5914259
    Abstract: Aminopeptidases originating from Aspergilli niger are disclosed, which can be produced in a fermentation broth or a liquid concentrate thereof substantially free of endoprotease. Such aminopeptidases may be advantageously employed in the food industry, e.g. in the preparation of bread doughs or cheese.
    Type: Grant
    Filed: January 30, 1997
    Date of Patent: June 22, 1999
    Assignee: Gist-brocades B.V.
    Inventors: Laurent Chevalet, Jerome Souppe, Joel De Leseleuc, Jacky Brunet, Martinus J.M. Warmerdam
  • Microbial strains producing sphingolipid bases
    Patent number: 5910425
    Abstract: Microbial strains capable of producing enhanced levels of sphingosine, dihydrosphingosine, phytosphingosine and/or derivatives thereof are disclosed. Additionally, there are disclosed methods based on mutagenesis, or other selection techniques, whereby such strains can be produced. As a preferred example thereof, mutant strains of Pichia are provided that are capable of producing about 50% more of such compounds than wild type strains.
    Type: Grant
    Filed: May 3, 1996
    Date of Patent: June 8, 1999
    Assignee: Gist-Brocades, N.V.
    Inventors: Lex De Boer, Ingrid Francisca Caroline Van Der Wildt
  • Enzymatic production of gluconic acid or its salts
    Patent number: 5897995
    Abstract: An enzymatic process for the conversion of glucose into gluconic acid, uses concentrated glucose solutions. The process employs a combination of glucose oxidase and catalase enzymes which may be obtained from an Aspergilltus niger strain and has a high ratio of catalase;glucose oxidase activity. The enzymatic process requires less time than conventional fermentation processes, the yield of the conversion is close to 100% and the obtained gluconic acid/gluconate solutions do not contain impurities.
    Type: Grant
    Filed: April 16, 1997
    Date of Patent: April 27, 1999
    Assignee: Gist-brocades, B.V.
    Inventors: Albert J. Vroemen, Marc Beverini
  • Method for influencing .beta.-lactam antibiotic production and for isolation of large quantities of ACV synthetase
    Patent number: 5882879
    Abstract: Novel methods and compositions are provided for the enhanced production of .beta.-lactam antibiotics. The process is exemplified by the production of penicillin. In addition, the P. chrysogenum and A. chrysogenum .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine synthetase genes have been isolated and sequenced. Also methods are provided for the production of .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine synthetase using recombined DNA techniques.
    Type: Grant
    Filed: April 4, 1994
    Date of Patent: March 16, 1999
    Assignee: Gist-Brocades, N.V.
    Inventors: Annemarie Veenstra, Juan Francisco Martin, Bruno Diez Garcia, Santiago Gutierez, Jose Luis Barredo, Eduardo Montenegro Prieto, Hans Von Doehren, Harriet Palissa, Henk Van Liempt
  • Strains of phaffia rhodozyma containing high levels of astaxanthin and low levels of 3-hydroxy-3', 4'-didehydro-.beta., .psi.-caroten-4-one (HDCO)
    Patent number: 5879927
    Abstract: The present invention provides Phaffia rhodozyma strains having increased levels of astaxantin and a low percentage of 3-hydroxy-3',4'-didehydro-.beta.,.psi.-caroten-4-one (HDCO). Such strains are obtained by a combination of mutagenesis and selection. The invention further provides a method for obtaining such strains. The invention also provides astaxanthin obtained from a mutagenized strain of Phaffia rhodozyma characterized in that it contains a decreased relative amount of HDCO.
    Type: Grant
    Filed: March 28, 1997
    Date of Patent: March 9, 1999
    Assignee: Gist-Brocades, B.V.
    Inventors: Lex De Boer, Bart Van Hell, Andreas Jacobus Johanna Krouwer
  • Selection marker gene free recombinant strains: method for obtaining them and the use of these strains
    Patent number: 5876988
    Abstract: The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
    Type: Grant
    Filed: June 23, 1997
    Date of Patent: March 2, 1999
    Assignee: Gist-brocades BV
    Inventors: Gerardus Cornelius Maria Selten, Bart Willem Swinkels, Robertus Franciscus Maria Van Gorcom
  • Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
    Patent number: 5863783
    Abstract: The present invention provides the cloning and expression of purified and isolated DNA molecules, obtainable from fungi, which encode enzymes having arabinan-degrading activity. The present invention also provides DNA constructs containing these DNA molecules and methods for their enhanced expression in selected microbial host cells. The present invention further provides methods for the use of the thus-produced arabinan-degrading enzymes.
    Type: Grant
    Filed: November 25, 1992
    Date of Patent: January 26, 1999
    Assignee: Gist-brocades, N.V.
    Inventors: Margaretha Van Heuvel, Janna Gardina Bakhuis, Yves Coutel, Abraham Harder, Leendert Hendrick De Graaff, Michel Johannes Anthonie Flipphi, Peter Van Der Veen, Jacob Visser, Peter Michael Andreoli
  • Cloning and expression of microbial phytase
    Patent number: 5863533
    Abstract: A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.
    Type: Grant
    Filed: April 10, 1995
    Date of Patent: January 26, 1999
    Assignee: Gist-brocades, N.V.
    Inventors: Robert F.M. Van Gorcom, Willem Van Hartingsveldt, Petrus Andreas Van Paridon, Annemarie Eveline Veenstra, Rudolf G.M. Luiten, Gerardus C.M. Selten