Patents Assigned to Gist-Brocades
  • Stable natamycin suspensions
    Patent number: 5552151
    Abstract: Concentrated suspensions of polyene fungicides such as natamycin are disclosed which exhibit good chemical, microbial and physical stability and which are thus particularly suitable as stock suspensions for the preparation of immersion liquids and coating emulsions for treatment of food, feed and agricultural products. Such suspensions have a pH in the range 3 to 4.8 and include a thickening agent.
    Type: Grant
    Filed: April 11, 1995
    Date of Patent: September 3, 1996
    Assignee: Gist-brocades B.V.
    Inventors: Bertus Noordam, Jacobus Stark, Ben R. De Haan, Hong Sheng Tan
  • Compositions and method for improving flour dough
    Patent number: 5547690
    Abstract: A method for improving the rheological properties of a flour dough through the use of an enzyme preparation which contains sulfhydryl oxidase and glucose oxidase.
    Type: Grant
    Filed: August 17, 1994
    Date of Patent: August 20, 1996
    Assignee: Gist-Brocades, N.V.
    Inventors: Seppo Vaisanen, Sampsa Haarasilta, Don Scott
  • Production of enzymes in seeds and their use
    Patent number: 5543576
    Abstract: A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
    Type: Grant
    Filed: November 2, 1993
    Date of Patent: August 6, 1996
    Assignees: Mogen International, Gist-Brocades
    Inventors: Albert J. J. van Ooijen, Krijn Rietveld, Andreas Hoekema, Jan Pen, Peter C. Sijmons, Teunis C. Verwoerd, Wilhemus J. Quax
  • Stabilization of carboxyl esterase
    Patent number: 5538876
    Abstract: Carboxyl esterase is inactivated by several chemical compounds such as naproxen or diclofop. By substituting or modifying certain basic residues of the carboxyl esterase, this enzyme shows improved stability properties during application. In this way it is possible to perform stereospecific hydrolysis reactions on industrial scale even at high substrate concentrations.
    Type: Grant
    Filed: August 23, 1995
    Date of Patent: July 23, 1996
    Assignee: Gist-brocades, N.V.
    Inventors: Johanna H.G.M. Mutsaers, Cornelis J. Van Der Laken, Cornelis P. Broekhuizen, Wilhemus J. Quax
  • Compositions and methods for making lipolytic enzymes
    Patent number: 5529917
    Abstract: Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.
    Type: Grant
    Filed: January 11, 1994
    Date of Patent: June 25, 1996
    Assignee: Gist-brocades
    Inventors: Peter M. Andreoli, Maria M. J. Cox, Farrokh Farin, Suzanne Wohlfarth-Rippel
  • Process for preparation of .beta.-lactams utilizing a combined concentration of acylating agent plus .beta.-lactam derivative of at least 400 mm
    Patent number: 5525483
    Abstract: .beta.-Lactam derivatives are synthesized by an enzymatic reaction of the parent amino .beta.-lactam with the corresponding acylating agent, the concentration of the acylating agent plus the concentration of .beta.-lactam derivative in the reaction mixture being above about 400 mM.
    Type: Grant
    Filed: January 18, 1994
    Date of Patent: June 11, 1996
    Assignee: Gist Brocades B.V.
    Inventors: Svend G. Kaasgaard, Ulla Veitland
  • N- and C-terminal truncation and deletion mutants of human interleukin-3
    Patent number: 5516512
    Abstract: Biologically active deletion and substitution mutants of hIL-3 are provided. Preferred mutants are those having one or more deletions at the N-terminus (amino acids 1-14) and/or the C-terminus (amino acids 116-133, 120-130 and/or 130-133). Preferred substitution mutants include Cys.sup.16 .fwdarw.Ala.sup.16 and/or Cys.sup.84 .fwdarw.Ala.sup.84, Glu.sup.50 .fwdarw.Lys.sup.50 and Lys.sup.79 .fwdarw.Glu.sup.79. These mutants can be used to formulate pharmaceutical compositions. Also disclosed are antibodies directed against specific epitopes localized between amino acids 29 and 54.
    Type: Grant
    Filed: November 8, 1993
    Date of Patent: May 14, 1996
    Assignee: Gist-brocades, N.V.
    Inventors: Lambertus C. J. Dorssers, Robert W. van Leen
  • Gram-negative alkaliphilic microorganisms
    Patent number: 5514575
    Abstract: Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various alkali-tolerant enzymes which may be used in various industrial processes requiring such enzymatic activity in a high pH environment.
    Type: Grant
    Filed: May 15, 1995
    Date of Patent: May 7, 1996
    Assignee: Gist-brocades, N.V.
    Inventors: Brian E. Jones, William D. Grant, Nadine C. Collins
  • Tortilla
    Patent number: 5510126
    Abstract: The quality of wheat flour tortillas is improved by using a yeast derivative in the tortilla dough.
    Type: Grant
    Filed: April 8, 1994
    Date of Patent: April 23, 1996
    Assignee: Gist-Brocades N.V.
    Inventors: Johannes H. Van Eijk, Merna Legel
  • Vectors for use in filamentous fungi
    Patent number: 5503991
    Abstract: Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as Aspergillus may be provided with foreign or natural coding regions associated with foreign or natural promoter sequences and optionally signal peptide sequences which can be used to control the expression and/or secretion of the proteins encoded by these coding regions.
    Type: Grant
    Filed: May 2, 1994
    Date of Patent: April 2, 1996
    Assignee: Gist-Brocades
    Inventors: David I. Gwynne, Francis P. Buxton, Mark H. Pickett, Roger W. Davies, Claudio Scazzocchio
  • Unit for the detection of residues of antibacterial compounds in liquids
    Patent number: 5494805
    Abstract: The detection of residues of antibacterials such as antibiotics and sulpha compounds in liquids such as milk, water, meat juice, serum or urine is disclosed. A test unit comprises an agar medium inoculated with a suitable test organism and two or more redox indicators.
    Type: Grant
    Filed: June 21, 1994
    Date of Patent: February 27, 1996
    Assignee: Gist-Brocades N.V.
    Inventors: Ferdinand T. Van Rijn, Robert Beukers, Johannes Kerkhof
  • Method for the preparation of certain .beta.-lactam antibiotics
    Patent number: 5470717
    Abstract: The present invention relates to an improved method for the preparation of certain .beta.-lactam antibiotics by enzymatic acylation or by deprotection of a protected intermediate.
    Type: Grant
    Filed: May 12, 1994
    Date of Patent: November 28, 1995
    Assignee: Gist-Brocades N.V.
    Inventor: Kim Clausen
  • Method and compositions for enhancing production of secondary metabolites using clustered biosynthetic genes
    Patent number: 5462862
    Abstract: Clustered antibiotic biosynthetic genes are employed for improvement of production of the antibiotic in microorganisms and for the isolation of other genes involved in the biosynthesis of the antibiotic. The invention is exemplified with improved production of penicillin in Penicillium chrysogenum, with the isolation of another clustered biosynthetic gene(s) and with the expression of penicillin biosynthetic genes in Acremonium chrysogenum.
    Type: Grant
    Filed: January 25, 1993
    Date of Patent: October 31, 1995
    Assignee: Gist-brocades N.V.
    Inventors: Martinus A. M. Groenen, Annemarie E. Veenstra, Pieter Van Solingen, Bertus P. Koekman, Lucia H. M. Van Der Voort, Juan F. Martin, Santiago Gutierrez, Bruno Diez, Emilio Alvarez, Jose L. Barredo, Christina Esmahan
  • Gram-negative alkaliphilic microorganisms
    Patent number: 5459062
    Abstract: Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various alkali-tolerant enzymes which may be used in various industrial processes requiring such enzymatic activity in a high pH environment.
    Type: Grant
    Filed: September 16, 1993
    Date of Patent: October 17, 1995
    Assignee: Gist-Brocades, N.V.
    Inventors: Brian E. Jones, William D. Grant, Nadine C. Collins
  • Mutated .beta.-lactam acylase genes
    Patent number: 5457032
    Abstract: New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.
    Type: Grant
    Filed: May 9, 1991
    Date of Patent: October 10, 1995
    Assignee: Gist-brocades NV
    Inventors: Wilhelmus J. Quax, Onno Misset, Jan M. Van Der Laan, Herman B. M. Lenting
  • Yeast derivative and method to improve bread quality
    Patent number: 5451413
    Abstract: The present invention relates to a method for improving the rheological properties of dough and quality of baked products by addition of a yeast derivative, preferably in combination with a reducing agent and/or enzyme preparations having amylase, hemicellulase, oxido reductase and/or protease activities.
    Type: Grant
    Filed: September 16, 1993
    Date of Patent: September 19, 1995
    Assignee: Gist-Brocades, B.V.
    Inventors: Jacob J. Fok, Jan D. R. Hille, Berend Van Der Veen
  • Large scale separation and purification of fermentation product
    Patent number: 5445949
    Abstract: A method of separating a hydrophobic fermentation product selected from the group consisting of lipase, esterase, endoxylanase and an antibiotic from a mixture comprising said product and contaminants which method comprises adding to the mixture sequentially(1) 0.5 to 15% (w/v) of a nonionic surfactant,(2) 0.5 to 60 mg of a flocculating agent per gram of said mixture,(3) 1 to 20% (w/v) of an extra nonionic surfactant,(4) a suitable K, Na, NH.sub.4 or Mg salt selected from the group of chlorides, sulfates, acetates, carbonates or phosphates, whereby the concentration of the salt is chosen so as to have the surfactant layer on top and is between 2 and 30%;to obtain a three phase product mixture, separating the product mixture into liquid-liquid-solid fractions and recovering the hydrophobic fermentation product.
    Type: Grant
    Filed: October 20, 1994
    Date of Patent: August 29, 1995
    Assignee: Gist-Brocades N.V.
    Inventors: Frans Koster, Jos J. P. Webbers
  • Cloning and expression of phytase from aspergillus
    Patent number: 5436156
    Abstract: A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.
    Type: Grant
    Filed: November 12, 1993
    Date of Patent: July 25, 1995
    Assignee: Gist-Brocades, N.V.
    Inventors: Robert F. M. Van Gorcom, Willem Van Hartingsveldt, Petrus A. Van Paridon, Annemarie E. Veenstra, Rudolf G. M. Luiten, Gerardus C. M. Selten
  • Detection of antibiotics
    Patent number: 5434053
    Abstract: A process for the detection of antibiotics in a liquid medium such as milk, urine and blood is disclosed which comprisesbringing together a fluid sample of the liquid medium, an labelled antibiotic binding protein, and an immobilized antibiotic,allowing the labelled antibiotic binding protein to bind with the immobilized antibiotic,removing labelled antibiotic binding protein which is not bound to immobilized antibiotic, anddetermining the amount of the labelled antibiotic binding protein bound to the immobilized antibiotic.
    Type: Grant
    Filed: October 6, 1993
    Date of Patent: July 18, 1995
    Assignee: Gist-Brocades N.V.
    Inventor: Roger Piasio
  • Cloning and expression of acetyl xylan esterases from fungal origin
    Patent number: 5426043
    Abstract: Methods and DNA constructs are provided for the expression of a fungal acetyl xylan esterase gene in microbial hosts. A purified fungal acetyl xylan esterase is obtained which is suited for the use as an accessory enzyme in the degradation of acetylated xylans.
    Type: Grant
    Filed: March 16, 1992
    Date of Patent: June 20, 1995
    Assignee: Gist-Brocades, N.V.
    Inventors: Leendert H. De Graaff, Jacob Visser, Henriette C. Van Den Broeck, Francois Strozyk, Felix J. M. Kormelink, Johannes C. P. Boonman