Patents Assigned to GIST (Gwangju Institue of Science and Technology)
  • Fungicide composition to prevent the growth of mould on foodstuff and agricultural products
    Patent number: 5597598
    Abstract: An antifungal composition is disclosed which comprises an antifungal agent of the polyene type, an acidific antifungal compound and an additional organic acid or its alkali or earth alkali salt. The use of this composition to treat food and agricultural products is disclosed.
    Type: Grant
    Filed: August 14, 1995
    Date of Patent: January 28, 1997
    Assignee: Gist-Brocades N.V.
    Inventors: Ferdinand T. J. van Rijn, Hong S. Tan, Martinus J. M. Warmerdam
  • Expression of phytase in plants
    Patent number: 5593963
    Abstract: The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.
    Type: Grant
    Filed: November 2, 1993
    Date of Patent: January 14, 1997
    Assignees: Mogen International, Gist-brocades, B.V.
    Inventors: Albert J. J. Van Ooijen, Krijn Rietveld, Andreas Hoekema, Jan Pen, Peter C. Sijmons, Teunis C. Verwoerd
  • Process for the production of juices from fruits and vegetables
    Patent number: 5578335
    Abstract: The present invention discloses the application of a pectin esterase substantially free from depolymerase activity in the maceration of apple pulp. The use of purified pectin esterase leads to a low amount of pectin degradation products in the juice and to the conservation of protopectin in the apple pomace. Furthermore, the juice yield and the pressability are increased through the use of purified PE.
    Type: Grant
    Filed: August 12, 1993
    Date of Patent: November 26, 1996
    Assignee: Gist-brocades, B.V.
    Inventors: Catherine M. T. Grassin, Pierre C. L. Fauquembergue
  • Process for obtaining aroma components and aromas from their precursors of a glycosidic nature, and aroma components and aromas thereby obtained
    Patent number: 5573926
    Abstract: A method is disclosed for obtaining aroma components and aromas from their glycosidic precursors containing a .beta.-apioside with the use of .beta.-apiosidase.
    Type: Grant
    Filed: July 18, 1994
    Date of Patent: November 12, 1996
    Assignees: Gist-brocades, N.V., Institute National de la Recherche Agronomique
    Inventors: Ziya Gunata, Sylvaine Bitteur, Raymond Baumes, Jean-Marc Brillouet, Claude Tapiero, Claude Bayonove, Robert Cordonnier
  • Gram-negative alkaliphilic microorganisms
    Patent number: 5571716
    Abstract: Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various alkali-tolerant enzymes which may be used in various industrial processes requiring such enzymatic activity in a high pH environment.
    Type: Grant
    Filed: April 8, 1993
    Date of Patent: November 5, 1996
    Assignee: Gist-brocades, N.V.
    Inventors: Brian E. Jones, William D. Grant, Nadine C. Collins
  • Stable natamycin suspensions
    Patent number: 5552151
    Abstract: Concentrated suspensions of polyene fungicides such as natamycin are disclosed which exhibit good chemical, microbial and physical stability and which are thus particularly suitable as stock suspensions for the preparation of immersion liquids and coating emulsions for treatment of food, feed and agricultural products. Such suspensions have a pH in the range 3 to 4.8 and include a thickening agent.
    Type: Grant
    Filed: April 11, 1995
    Date of Patent: September 3, 1996
    Assignee: Gist-brocades B.V.
    Inventors: Bertus Noordam, Jacobus Stark, Ben R. De Haan, Hong Sheng Tan
  • Compositions and method for improving flour dough
    Patent number: 5547690
    Abstract: A method for improving the rheological properties of a flour dough through the use of an enzyme preparation which contains sulfhydryl oxidase and glucose oxidase.
    Type: Grant
    Filed: August 17, 1994
    Date of Patent: August 20, 1996
    Assignee: Gist-Brocades, N.V.
    Inventors: Seppo Vaisanen, Sampsa Haarasilta, Don Scott
  • Production of enzymes in seeds and their use
    Patent number: 5543576
    Abstract: A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
    Type: Grant
    Filed: November 2, 1993
    Date of Patent: August 6, 1996
    Assignees: Mogen International, Gist-Brocades
    Inventors: Albert J. J. van Ooijen, Krijn Rietveld, Andreas Hoekema, Jan Pen, Peter C. Sijmons, Teunis C. Verwoerd, Wilhemus J. Quax
  • Stabilization of carboxyl esterase
    Patent number: 5538876
    Abstract: Carboxyl esterase is inactivated by several chemical compounds such as naproxen or diclofop. By substituting or modifying certain basic residues of the carboxyl esterase, this enzyme shows improved stability properties during application. In this way it is possible to perform stereospecific hydrolysis reactions on industrial scale even at high substrate concentrations.
    Type: Grant
    Filed: August 23, 1995
    Date of Patent: July 23, 1996
    Assignee: Gist-brocades, N.V.
    Inventors: Johanna H.G.M. Mutsaers, Cornelis J. Van Der Laken, Cornelis P. Broekhuizen, Wilhemus J. Quax
  • Compositions and methods for making lipolytic enzymes
    Patent number: 5529917
    Abstract: Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.
    Type: Grant
    Filed: January 11, 1994
    Date of Patent: June 25, 1996
    Assignee: Gist-brocades
    Inventors: Peter M. Andreoli, Maria M. J. Cox, Farrokh Farin, Suzanne Wohlfarth-Rippel
  • Process for preparation of .beta.-lactams utilizing a combined concentration of acylating agent plus .beta.-lactam derivative of at least 400 mm
    Patent number: 5525483
    Abstract: .beta.-Lactam derivatives are synthesized by an enzymatic reaction of the parent amino .beta.-lactam with the corresponding acylating agent, the concentration of the acylating agent plus the concentration of .beta.-lactam derivative in the reaction mixture being above about 400 mM.
    Type: Grant
    Filed: January 18, 1994
    Date of Patent: June 11, 1996
    Assignee: Gist Brocades B.V.
    Inventors: Svend G. Kaasgaard, Ulla Veitland
  • N- and C-terminal truncation and deletion mutants of human interleukin-3
    Patent number: 5516512
    Abstract: Biologically active deletion and substitution mutants of hIL-3 are provided. Preferred mutants are those having one or more deletions at the N-terminus (amino acids 1-14) and/or the C-terminus (amino acids 116-133, 120-130 and/or 130-133). Preferred substitution mutants include Cys.sup.16 .fwdarw.Ala.sup.16 and/or Cys.sup.84 .fwdarw.Ala.sup.84, Glu.sup.50 .fwdarw.Lys.sup.50 and Lys.sup.79 .fwdarw.Glu.sup.79. These mutants can be used to formulate pharmaceutical compositions. Also disclosed are antibodies directed against specific epitopes localized between amino acids 29 and 54.
    Type: Grant
    Filed: November 8, 1993
    Date of Patent: May 14, 1996
    Assignee: Gist-brocades, N.V.
    Inventors: Lambertus C. J. Dorssers, Robert W. van Leen
  • Gram-negative alkaliphilic microorganisms
    Patent number: 5514575
    Abstract: Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various alkali-tolerant enzymes which may be used in various industrial processes requiring such enzymatic activity in a high pH environment.
    Type: Grant
    Filed: May 15, 1995
    Date of Patent: May 7, 1996
    Assignee: Gist-brocades, N.V.
    Inventors: Brian E. Jones, William D. Grant, Nadine C. Collins
  • Tortilla
    Patent number: 5510126
    Abstract: The quality of wheat flour tortillas is improved by using a yeast derivative in the tortilla dough.
    Type: Grant
    Filed: April 8, 1994
    Date of Patent: April 23, 1996
    Assignee: Gist-Brocades N.V.
    Inventors: Johannes H. Van Eijk, Merna Legel
  • Vectors for use in filamentous fungi
    Patent number: 5503991
    Abstract: Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as Aspergillus may be provided with foreign or natural coding regions associated with foreign or natural promoter sequences and optionally signal peptide sequences which can be used to control the expression and/or secretion of the proteins encoded by these coding regions.
    Type: Grant
    Filed: May 2, 1994
    Date of Patent: April 2, 1996
    Assignee: Gist-Brocades
    Inventors: David I. Gwynne, Francis P. Buxton, Mark H. Pickett, Roger W. Davies, Claudio Scazzocchio
  • Unit for the detection of residues of antibacterial compounds in liquids
    Patent number: 5494805
    Abstract: The detection of residues of antibacterials such as antibiotics and sulpha compounds in liquids such as milk, water, meat juice, serum or urine is disclosed. A test unit comprises an agar medium inoculated with a suitable test organism and two or more redox indicators.
    Type: Grant
    Filed: June 21, 1994
    Date of Patent: February 27, 1996
    Assignee: Gist-Brocades N.V.
    Inventors: Ferdinand T. Van Rijn, Robert Beukers, Johannes Kerkhof
  • Method for the preparation of certain .beta.-lactam antibiotics
    Patent number: 5470717
    Abstract: The present invention relates to an improved method for the preparation of certain .beta.-lactam antibiotics by enzymatic acylation or by deprotection of a protected intermediate.
    Type: Grant
    Filed: May 12, 1994
    Date of Patent: November 28, 1995
    Assignee: Gist-Brocades N.V.
    Inventor: Kim Clausen
  • Method and compositions for enhancing production of secondary metabolites using clustered biosynthetic genes
    Patent number: 5462862
    Abstract: Clustered antibiotic biosynthetic genes are employed for improvement of production of the antibiotic in microorganisms and for the isolation of other genes involved in the biosynthesis of the antibiotic. The invention is exemplified with improved production of penicillin in Penicillium chrysogenum, with the isolation of another clustered biosynthetic gene(s) and with the expression of penicillin biosynthetic genes in Acremonium chrysogenum.
    Type: Grant
    Filed: January 25, 1993
    Date of Patent: October 31, 1995
    Assignee: Gist-brocades N.V.
    Inventors: Martinus A. M. Groenen, Annemarie E. Veenstra, Pieter Van Solingen, Bertus P. Koekman, Lucia H. M. Van Der Voort, Juan F. Martin, Santiago Gutierrez, Bruno Diez, Emilio Alvarez, Jose L. Barredo, Christina Esmahan
  • Gram-negative alkaliphilic microorganisms
    Patent number: 5459062
    Abstract: Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various alkali-tolerant enzymes which may be used in various industrial processes requiring such enzymatic activity in a high pH environment.
    Type: Grant
    Filed: September 16, 1993
    Date of Patent: October 17, 1995
    Assignee: Gist-Brocades, N.V.
    Inventors: Brian E. Jones, William D. Grant, Nadine C. Collins
  • Mutated .beta.-lactam acylase genes
    Patent number: 5457032
    Abstract: New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.
    Type: Grant
    Filed: May 9, 1991
    Date of Patent: October 10, 1995
    Assignee: Gist-brocades NV
    Inventors: Wilhelmus J. Quax, Onno Misset, Jan M. Van Der Laan, Herman B. M. Lenting