Patents Assigned to GIST (Gwangju Institue of Science and Technology)
  • Expression of phytase in plants
    Patent number: 5770413
    Abstract: The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.
    Type: Grant
    Filed: August 7, 1996
    Date of Patent: June 23, 1998
    Assignees: Gist-brocades, B.V., Mogen International
    Inventors: Albert J. J. Van Ooijen, Krijn Rietveld, Andreas Hoekema, Jan Pen, Peter Christian Sijmons, Teunis Cornelis Verwoerd
  • Process for the synthesis of amikacin
    Patent number: 5763587
    Abstract: In the synthesis of amikacin, having the formula: ##STR1## by acylation of a diprotected derivative of kanamycin A with a reactive derivative of L-(-)-4-amino-2-hydroxybutyric acid, the selection of the reaction solvent and of the pH conditions permit the industrial economically and the synthesis yield to be improved.
    Type: Grant
    Filed: February 14, 1992
    Date of Patent: June 9, 1998
    Assignee: Gist Brocades Italy Spa
    Inventor: Alberto Mangia
  • Method to alter the properties of acetylated xylan
    Patent number: 5763260
    Abstract: Methods and DNA constructs are provided for the expression of a fungal acetyl xylan esterase gene in microbial hosts. A purified fungal acetyl xylan esterase is obtained which is suited for the use as an accessory enzyme in the degradation of acetylated xylans.
    Type: Grant
    Filed: March 8, 1995
    Date of Patent: June 9, 1998
    Assignee: Gist-brocades, B.V.
    Inventors: Leendert H. De Graaff, Jacob Visser, Henriette C. Van Den Broeck, Francois Strozyk, Felix J. M. Kormelink, Johannes C. P. Boonman
  • Diamine salts of clavulanic acid
    Patent number: 5760218
    Abstract: New diamine salts of clavulanic acid, pharmaceutical compositions comprising the same, and a new process using these diamine salts in the preparation of clavulanic acid and salts and esters thereof have been provided.
    Type: Grant
    Filed: May 31, 1995
    Date of Patent: June 2, 1998
    Assignee: Gist-Brocades N.V.
    Inventor: Pieter Gijsbert Weber
  • Process for the fractioning and recovery of valuable compounds from vinasse produced in fermentations
    Patent number: 5760078
    Abstract: A process is provided for the preparation of valuable products from vinasse which contains potassium salts. The process comprises concentrating the vinasse to a dry matter content of 50-80% dry solids and separating the formed crystals in a concentration step to form a supernatant which is free of potassium salts. Ammonium sulphate is added to the vinasse before or during concentrating the vinasse.Furthermore, clarification of the supernatant is carried out to provide for the separation of betaine, PCA, succinic acid or citric acid. Filtration, or microfiltration is used for the clarification and a chromatographic separation is used for the separation of betaine as well as the other products. Potassium salts are obtained from the crystals to provide for a fertilizer.
    Type: Grant
    Filed: April 3, 1996
    Date of Patent: June 2, 1998
    Assignee: Gist-Brocades B.V.
    Inventors: Reinder Sietze Hamstra, Peter Johannes Schoppink
  • Oxido reductase enzyme system obtainable from P. chrysogenum, the set of genes encoding the same and the use of oxido reductase enzyme systems or genes encoding the same for increasing antibiotic production
    Patent number: 5753435
    Abstract: A new oxido reductase enzyme activity obtainable from P. chrysogenum, involved in the production of .beta.-lactams, the set of genes encoding said enzyme activity, and a method to enhance said production by using the above-mentioned oxido reductase system and other oxido reductase systems or genes encoding for the same are disclosed.
    Type: Grant
    Filed: February 10, 1995
    Date of Patent: May 19, 1998
    Assignee: Gist-Brocades
    Inventors: Yair Aharonowitz, Lucia Helena Maria Van Der Voort, Gerald Cohen, Reolof Ary Lans Bovenberg, Rachel Schreiber, Anat Argaman, Yossef Av-Gay, Helena Maria Nan, Alfred Kattevilder, Harriet Palissa, Henk Van Liempt
  • Acylation method for penicillins and cephalosporins
    Patent number: 5753458
    Abstract: A method for providing a semisynthetic .beta.-lactam antibiotic by enzyme catalyzed acylation of the parent .beta.-lactam with an activated derivative of the side chain acid wherein a modulator, which consists of one or more compounds different from the reactants and the reaction product and which suppresses the hydrolysis of the activated derivative of the side chain acid and the desired product more than it suppresses the synthesis of the desired product, is added to the reaction mixture, at the beginning of the reaction process, in a concentration from about 0.2 to 100.times.10.sup.3 .mu.m.
    Type: Grant
    Filed: February 7, 1996
    Date of Patent: May 19, 1998
    Assignee: Gist-Brocades B.V.
    Inventors: Kim Clausen, Annette Nielsen, Niels Petersen, Alexander Nikolov
  • DNA comprising regulatory regions from gene y of penicillium chrysogenum
    Patent number: 5747285
    Abstract: A gene expression system and a protein secretion system for eukaryotic, especially fungal, microorganisms has been provided for.
    Type: Grant
    Filed: March 8, 1994
    Date of Patent: May 5, 1998
    Assignee: Gist-Brocades, NV.
    Inventors: Roelof Ary Lans Bovenberg, Adrianus Wilhelmus Hermanus Vollebregt, Pieter Van Solingen
  • Longterm antimicrobial activity obtained by sustained release of hydrogen peroxide
    Patent number: 5747078
    Abstract: Methods for long-term preservation of food products using a lactoperoxidase system. Long-term preservation is achieved by controlled generation of hydrogen peroxide as a substrate for the lactoperoxidase system. Controlled production of hydrogen peroxide is achieved by generating the hydrogen peroxide enzymatically form a system wherein the substrate for the enzyme generating the hydrogen peroxide is immobilized or is generated in situ from a precursor.
    Type: Grant
    Filed: January 13, 1995
    Date of Patent: May 5, 1998
    Assignee: Gist-brocades, N.V.
    Inventors: Sylvia Josefine De Jong, Ben Rudolf De Haan, Hong Sheng Tan
  • Diamine salts for purification of clavulanic acid
    Patent number: 5741903
    Abstract: A process for the purification of clavulanic acid comprising reacting in an organic solvent impure clavulanic acid with a diamine of the formula ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are individually selected from the group consisting of alkyl of 1 to 8 carbon atoms, cycloalkyl of 3 to 8 carbon atoms and cycloalkyl alkyl of 3 to 8 cycloalkyl carbon atoms and alkyl of 1 to 8 carbon atoms, all optionally substituted with 1 to 3 members of the group consisting of halogen, --OH, lower alkoxy and carboxy esterified with lower alkyl or R.sub.1 and R.sub.2 or R.sub.3 and R.sub.4 taken with the nitrogen to which they are attached form piperidino and n is an integer from 1 to 10 to form an amine salt selected from the group consisting of ##STR2## recovering the amine salts and acidifying the amine salt to recover purified clavulanic acid.
    Type: Grant
    Filed: March 26, 1993
    Date of Patent: April 21, 1998
    Assignee: Gist-Brocades N.V.
    Inventor: P. G. Weber
  • Stable gene amplification in chromosomal DNA of prokaryotic microorganisms
    Patent number: 5733723
    Abstract: Transformed prokaryotic hosts are provided comprising two or more copies of a DNA sequence stably maintained in their chromosome, said DNA sequence comprising a gene encoding a polypeptide of interest wherein said copies are separated by endogenous chromosomal DNA sequences. Methods are also provided for producing said transformed host strains. The transformed host strains are capable of increased production of the polypeptide of interest compared to host strains which already produce said polypeptide. Preferred host strains are Bacillus novo species PB92 which produces a high-alkaline proteolytic enzyme and B. licheniformis strain T5 which produces a thermostable .alpha.-amylase, and mutants and variants of said strains. Preferred polypeptide encoding genes are the protease encoding gene obtainable from Bacillus PB92 and the .alpha.-amylase encoding gene obtainable from B. licheniformis strain T5.
    Type: Grant
    Filed: August 24, 1994
    Date of Patent: March 31, 1998
    Assignee: Gist-Brocades
    Inventors: Christiaan A. G. van Eekelen, Johannes C. van der Laan, Leo J. S. M. Mulleners
  • Process for the production of 7-ADCA via expandase activity on penicillin G
    Patent number: 5731165
    Abstract: An overall process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a Penicillium chrysogenum transformant strain expressing expandase.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: March 24, 1998
    Assignee: Gist-brocades, B.V.
    Inventors: Roelof Ary Lans Bovenberg, Bertus Pieter Koekman, Dirk Schipper, Adrianus Wilhelmus Vollebregt
  • Vectors for use in filamentous fungi
    Patent number: 5728547
    Abstract: Novel vectors are disclosed for use in filamentous fungi such as Aspergillus sp. in particular, whereby protein coding regions may be inserted therein to achieve expression or expression followed by secretion of the coded protein from the host. Signal peptide sequences and promoter sequences valuable for this purpose are disclosed as are expression vectors containing coding regions native or foreign to the fungal host. In accordance with the invention, a filamentous fungus such as Aspergillus may be provided with foreign or natural coding regions associated with foreign or natural promoter sequences and optionally signal peptide sequences which can be used to control the expression and/or secretion of the proteins encoded by these coding regions.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: March 17, 1998
    Assignee: Royal Gist-Brocades N.V.
    Inventors: David Ivor Gwynne, Francis Paul Buxton, Mark Hudson Pickett, Roger Wayne Davies, Claudio Scazzocchio
  • Process for the efficient production of 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA and 3-(carboxymethylthio)propionyl-7-ADCA
    Patent number: 5726032
    Abstract: An overall efficient process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via 2-(carboxyethylthio)acetyl- and 3-(carboxymethylthio)propionyl-7-ADCA, using a Penicillium chrysogenum transformant strain expressing expandase in conjunction with acyltransferase, is provided.
    Type: Grant
    Filed: April 4, 1996
    Date of Patent: March 10, 1998
    Assignee: Gist-Brocades B.V.
    Inventors: Roelof Ary Lans Bovenberg, Bertus Pieter Koekman, Andreas Hoekema, Jan Metske Van Der Laan, Jan Verweij, Erik De Vroom
  • Diamine salts of clavulanic acid
    Patent number: 5719277
    Abstract: New diamine mono and diamine di salts of clavulanic acid, pharmaceutical compositions comprising the same, and a new process using these diamine salts in the preparation of clavulanic acid and salts and esters thereof have been provided.
    Type: Grant
    Filed: June 1, 1995
    Date of Patent: February 17, 1998
    Assignee: Gist-Brocades N.V.
    Inventor: Pieter G. Weber
  • Gram-negative alkaliphilic microorganisms
    Patent number: 5719047
    Abstract: Gram-negative bacteria, which are obligate alkaliphiles, have been isolated from samples of soil, water and sediment and a number of other sources obtained from in and around soda lakes. These bacteria have been analyzed according to the principles of numerical taxonomy with respect to each other, as well as to a variety of known bacteria. In addition, these bacteria are further circumscribed by an analysis of various chemotaxonomic characteristics. The bacteria produce various alkali-tolerant enzymes which may be used in various industrial processes requiring such enzymatic activity in a high pH environment.
    Type: Grant
    Filed: May 15, 1995
    Date of Patent: February 17, 1998
    Assignee: Gist-brocades, N.V.
    Inventors: Brian Edward Jones, William Duncan Grant, Nadine Claire Collins
  • Method for the preparation of feed pellets
    Patent number: 5716655
    Abstract: The present invention discloses a method for obtaining feed pellets. The method comprises the addition of active ingredients to feed pellets after the pellets have been extruded. The method further comprises the addition of a solution or suspension of the desired feed or ingredient in a water or oil phase to the pellets under reduced pressure and subsequently increasing the pressure. The method results in pellets with a high degree of loading and in which the active ingredients are homogeneously distributed.
    Type: Grant
    Filed: October 20, 1993
    Date of Patent: February 10, 1998
    Assignee: Gist-Brocades B.V.
    Inventors: Reinder Sietze Hamstra, Augustinus Franciscus Tromp
  • Dry yeast compositions and processes for preparing the same
    Patent number: 5716654
    Abstract: Dry granular yeast compositions for use in the production of bakery products and beverages. In particular, a dry granular yeast composition having a moisture content of not more than about 8% (w/w) and containing about 0.1 to 8% (w/w) of a bread-improving agent, wherein the dry yeast is present in granular form and said bread-improving agent is a coating on the dry yeast granules in the form of a film or adhered particles.
    Type: Grant
    Filed: December 23, 1994
    Date of Patent: February 10, 1998
    Assignee: Gist-brocades, N.V.
    Inventor: Jan Willem Groenendaal
  • Transformed industrial bacillus strains and methods for making and using them
    Patent number: 5716807
    Abstract: Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected. Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.
    Type: Grant
    Filed: August 16, 1996
    Date of Patent: February 10, 1998
    Assignee: Gist-Brocades
    Inventors: Johan Pieter Marinus Sanders, Johannes Abel Van den Berg, Peter Michael Andreoli, Yvonne Johanna Vos, Jan Hendrik van Ee, Leo J. S. M. Mulleners
  • Astaxanthin-producing yeast cells methods for their preparation and their use
    Patent number: 5712110
    Abstract: An isolated pure culture of a strain of Phaffia rhodozyma which produces astaxanthin in an amount of at least 600 .mu.g per g Phaffia rhodozyma dry matter, as determined by HPLC analysis.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: January 27, 1998
    Assignee: Gist-brocades, B.V.
    Inventors: Bent Flen.o slashed., Ib Christensen, Robert Larsen, Steffen Radich Johansen, Eric A. Johnson