Patents Assigned to Health and Human Services, National Institutes of Health
  • Publication number: 20030114375
    Abstract: The present invention provides a method of identifying CFTR-binding compounds for treating cells having a reduced apical Cl− conductance, such as cystic fibrosis cells. This identification method involves the use of polypeptide I&agr;, which constitutes a portion of the CFTR protein. The present invention also provides a method of treating CF cells by contacting cells having a reduced apical Cl− conductance with a therapeutically effective quantity of a compound selected by the present inventive identification method. Preferred compounds for such treatment have little or no affinity for adenosine cell receptors. The present invention provides novel compounds useful in practicing the present inventive method, as well as pharmaceutical compositions containing such compounds.
    Type: Application
    Filed: July 3, 2002
    Publication date: June 19, 2003
    Applicant: The Government of the USA, Dept of Health and Human Services, The National Institutes of Health
    Inventors: Harvey B. Pollard, Kenneth A. Jacobson
  • Publication number: 20030027311
    Abstract: This invention provides for new recombinant ribonuclease proteins which are active when expressed by bacteria. This allows the recombinant ribonucleases of this invention to be fused in-frame with ligand binding moieties to form cytotoxic fusion proteins. Furthermore, these proteins are more active than ribonucleases currently available even though the proteins of this invention lack an N-terminal pyroglutamic acid, which has been found to be necessary for ribonucleolytic activity. Because these proteins are recombinant proteins, mutations which increase cytotoxicity can be engineered.
    Type: Application
    Filed: September 6, 2001
    Publication date: February 6, 2003
    Applicant: The Department of Health and Human Services National Institutes of Health
    Inventors: Susanna M. Rybak, Dianne L. Newton
  • Publication number: 20030008322
    Abstract: Laser capture microdissection occurs where the transfer polymer film is placed on a substrate overlying visualized and selected cellular material from a sample for extraction. The transfer polymer film is focally activated (melted) with a pulse brief enough to allow the melted volume to be confined to that polymer directly irradiated. This invention uses brief pulses to reduce the thermal diffusion into surrounding non-irradiated polymer, preventing it from being heated hot enough to melt while providing sufficient heat by direct absorption in the small focal volume directly irradiated by the focused laser beam. This method can be used both in previously disclosed contact LCM, non contact LCM, using either condenser-side (or beam passes through polymer before tissue) or epi-irradiation (or laser passes through tissue before polymer). It can be used in configuration in which laser passes through tissue before polymer with and without an additional rigid substrate.
    Type: Application
    Filed: April 8, 2002
    Publication date: January 9, 2003
    Applicant: Dept. of Health & Human Services, NATIONAL INSTITUTES OF HEALTH Ofc. OF TECHNOLOGY TRANSFER
    Inventors: Robert F. Bonner, Seth R. Goldstein, Paul D. Smith, Thomas J. Pohida
  • Patent number: 5705163
    Abstract: A target-specific, cytotoxic, recombinant Pseudomonas exotoxin is described. Such toxins are made by inserting specific recognition molecules at specific cloning sites in at least domain III near the carboxyl terminus of the PE molecule. Various modifications of the carboxyl terminus of the PE molecule to increase cytotoxicity are set forth. Multifunctional, recombinant, cytotoxic fusion proteins containing at least two different recognition molecules are provided for killing cells expressing receptors to which the recognition molecules bind with specificity. Methods for producing novel recombinant PE molecules with specific properties are described.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: January 6, 1998
    Assignee: The United States of America as represented by the Department of Health and Human Services, National Institutes of Health
    Inventors: Ira Pastan, Vijay K. Chaudhary, David Fitzgerald