Abstract: A microscopy assembly includes: a stage for receiving an optical cartridge, the stage comprising: a platform defining a planar surface and a window extending through the platform, and a retention arm defining a retention face and a rounded release face opposite the retention face, wherein the retention face at least partially faces the planar surface of the platform and is configured to engage a complementary cartridge engagement surface of the optical cartridge inserted into the microscope assembly.

Abstract: A microscopy assembly may comprise an objective lens defining an imaging path axis, one or more light sources, and a microscopy body, wherein the microscopy body defines an objective optical pathway having an objective optical axis, the monolithic microscopy body defines a light source optical pathway having a light source optical axis, the light source optical axis is transverse to the objective optical axis, the objective lens is optically coupled to the objective optical pathway, and each light source of the one or more light sources is optically coupled to the light source pathway.

Abstract: The present disclosure is directed to assemblies and methods for heating a biological cartridge in a microscope assembly. The assembly includes a stage for receiving a cartridge holding a biological sample, the cartridge having a top face, a bottom face, and defining a sample chamber that at least partially encapsulates the biological sample, an image detector in optical communication with the stage, a controller communicatively coupled to the image detector, and a heating element communicatively coupled to the controller, wherein the heating element defines a perimeter surrounding a sample chamber of the cartridge with the cartridge positioned on the stage.

Abstract: A cartridge for imaging a biological sample is disclosed. The cartridge includes a housing including: (i) a port and (ii) a recess for receiving the biological sample. The cartridge additionally includes a gasket surrounding at least a of portion the recess of the housing, wherein the gasket includes at least one wall defining a boundary of a reservoir, wherein the reservoir includes the recess, and wherein the reservoir is in fluid communication with the port. The cartridge further includes a sheet interfaced with the at least one wall of the gasket, wherein when the at least one wall is interfaced with the sheet, the reservoir is defined as an area between the at least one wall, the recess of the housing, and a surface of the sheet, and wherein the sheet is optically transparent.

Abstract: A digital microscope and a method to use the same include an imaging sensor defining a microscope optical axis, a light source spaced apart from the imaging sensor, a diffuser lens optically coupled to the light source, a mask optically coupled to the diffuser, a sample holder to hold a cartridge positioned between the imaging sensor and the light source, and a controller communicatively coupled to the imaging sensor. The mask restricts the passage of light beams outside of a range between about 8 degrees and about 40 degrees with respect to the microscope optical axis. The controller includes a processor and a memory comprising instructions that, when executed by the processor, cause the processor to capture a plurality of images of a liquid sample in the cartridge with the imaging sensor, and identify one or more constituents within the liquid sample based on the plurality of images.

Abstract: Methods for detecting the presence or absence of an intestinal parasite infection in a mammalian sample are disclosed herein. The methods can be used to confirm the presence or absence of an intestinal parasite by applying a first test that is an immunoassay or a nucleic acid assay and conducting a second test such as microscopic evaluation assay based on the outcome of the first test or based on one or more evaluation metrics. Confirmation of the presence or absence of the intestinal parasite in the mammal can be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated.

Abstract: A point-of-care analyzer is provided including an analysis unit that receives a biological sample for analysis, the analysis unit including an interrogation device; a housing at least partially surrounding the analysis unit, the housing defining a cartridge slot for receiving a cartridge comprising the biological sample; and a tray on the housing, the tray comprising an upper surface for holding the cartridge and one or more reagent holders for holding a consumable reagent.

Abstract: Systems and methods for use with a biological sample include a microscopy device, a diluent, a sample holder, and one or more of lyophilized cakes comprising artificial particles. The lyophilized cakes and the biological sample are mixed with the diluent in the sample holder to form a solution. The biological sample is imaged with the artificial particles as reference markers using the microscopy device. A settling time of the artificial particles is shorter than or equal to a settling time of the biological sample.

Abstract: The present disclosure is directed to methods and systems for verifying biological analysis devices. The method includes calibrating a hematology device utilizing at least one of a quality control sample or historical patient data, generating diagnostic data associated with a portion of a biological sample with the hematology device, the diagnostic data comprising a first group of attributes associated with the biological sample, identifying whether the first group of attributes is within predetermined parameters, capturing an image of another portion of the biological sample with a microscopy device, identifying a second group of attributes associated with the biological sample based at least in part on the image, and determining one or more verification parameters based on the second group of attributes.

Abstract: A collection device for examining fecal matter to determine whether a patient is afflicted with parasites includes a particle accumulating plug mounted on an end of a collection tube containing a fecal specimen. The particle accumulating plug includes an inner wall that defines an inverted funnel. A pipetting port is formed through the inner wall at the apex of the inverted funnel allowing the tip of a pipette to enter the port and aspirate the ova or eggs, or other parasite cells, that have separated from the fecal specimen. The conical shape of the inner wall of the plug directs the separated parasite ova or eggs towards the pipetting port and to accumulate within the volume of the funnel of the plug so that a desired volume of fluid containing a concentrated quantity of parasite ova or eggs may be aspirated into the pipette tip through the pipetting port.

Abstract: This invention provides reagents and methods for diagnosing renal disease. Differential levels of inosine metabolite, and proteins: apolipoprotein C-I, apolipoprotein C-II, fibrinogen alpha chain, or fibrinogen A-alpha chain, kininogen, Inter-Alpha Inhibitor H4 (ITIH4), keratin Type I cytoskeletol 10 cystatin A, cystatin B and other polypeptides and fragments thereof provide biomarkers of renal disease and are described herein.

Abstract: A collection device for examining fecal matter to determine whether a patient is afflicted with parasites includes a particle accumulating plug mounted on an end of a collection tube containing a fecal specimen. The particle accumulating plug includes an inner wall that defines an inverted funnel. A pipetting port is formed through the inner wall at the apex of the inverted funnel allowing the tip of a pipette to enter the port and aspirate the ova or eggs, or other parasite cells, that have separated from the fecal specimen. The conical shape of the inner wall of the plug directs the separated parasite ova or eggs towards the pipetting port and to accumulate within the volume of the funnel of the plug so that a desired volume of fluid containing a concentrated quantity of parasite ova or eggs may be aspirated into the pipette tip through the pipetting port.

Abstract: An example method includes receiving a first order to conduct a first diagnostic test of a sample from a patient, sending the first order to the first diagnostic testing instrument for input into a queue of the first diagnostic testing instrument, receiving alerts and status of the diagnostic test as testing is performed and for indication of completion of the first diagnostic test, sending the alerts and status of the diagnostic test to a second computing device for notice to a technician, receiving an output of the first diagnostic test, generating a recommendation of follow-on testing to perform based on the output of the first diagnostic test, receiving confirmation to perform the follow-on testing, generating a second order to conduct a second diagnostic test of another sample from the patient, and sending the second order to the second diagnostic testing instrument for input into a queue for the second diagnostic testing instrument.

Abstract: An example method includes capturing, via an x-ray machine, a plurality of x-ray images of a patient covering a number of different anatomy of the patient in any order, using a machine learning algorithm to process the plurality of x-ray images for identification of an anatomy in respective x-ray images of the plurality of x-ray images, associating a label with each of the plurality of x-ray images based on the identification of the anatomy, positioning each of the plurality of x-ray images upright based on a preset coordinate scheme for the anatomy, arranging the plurality of x-ray images into a predetermined order based on the species of the patient, and generating and outputting a data file including the plurality of x-ray images in the predetermined order, positioned upright, and labeled.

Abstract: A method of measuring the degree of Z-axis variability in a chemical reagent test slide, which includes the steps of placing a Z-axis measurement fixture on a surface of a gauge block of an optical measurement system having a camera, mounting the chemical reagent test slide on the Z-axis measurement fixture, and positioning the chemical reagent test slide mounted on the Z-axis measurement fixture in optical communication with the camera.

Abstract: Provided herein are compositions and methods for detection of detection and treatment of Mycoplasma haemofelis and Mycoplasma haemocanis.

Abstract: The disclosure relates generally to methods for measuring bacteria in biological samples. More particularly, the disclosure relates to methods for measuring bacteria using an aqueous clearance solution.

Abstract: An example computer-implemented method of increasing veterinary health care compliance includes receiving information indicating that an animal patient concluded a visit at a veterinary practice, in response accessing diagnostic test results for the animal patient, based on the diagnostic test results creating a pet owner profile for an owner of the animal patient, matching the pet owner profile with a stored message and stored information relating to a product associated with recommended treatment for the animal patient, sending an electronic message to a second computing device associated with the owner of the animal patient that includes the stored message and the stored information relating to the product associated with recommended treatment for the animal patient, receiving a report indicating whether the owner of the animal patient obtained the product associated with recommended treatment for the animal patient, and updating the pet owner profile to be indicative of the report.

Abstract: A system and method for retrieving data from a data generating device includes a communication intermediary and an external data storage system. The communication intermediary has a first network interface that is connected to the external data storage system and a second network interface that is connected to the data generating device. The communication intermediary isolates the network communication between the data generating device and second network interface from the network communication between the external storage system and the first network interface, and thereby isolates the data generating device from the external storage system. The communication intermediary monitors the data generating device for newly generated data files, copies the newly generated data files to an internal memory and sends the data files to the external storage system.