Patents Assigned to Inscripta, Inc.
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10584333
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: October 29, 2019
    Date of Patent: March 10, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10584334
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: March 10, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10576474
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: October 9, 2019
    Date of Patent: March 3, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10557216
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: February 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Automated nucleic acid assembly and introduction of nucleic acids into cells
    Patent number: 10557150
    Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.
    Type: Grant
    Filed: September 14, 2019
    Date of Patent: February 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10550363
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: February 4, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10533152
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: January 14, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Bruce Chabansky, Jorge Bernate, Andrew Garst, Richard Fox
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10532324
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: August 14, 2019
    Date of Patent: January 14, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Andrew Garst, Richard Fox, Matthew Estes, Bruce Chabansky
  • Methods for identifying T-cell receptor antigens
    Patent number: 10526598
    Abstract: The present disclosure automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptide antigens to identify potential TCR binding targets. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: January 7, 2020
    Assignee: Inscripta, Inc.
    Inventors: Deanna Church, Stephen Federowicz, Michael Graige
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10519437
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: September 14, 2019
    Date of Patent: December 31, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Methods for identifying selective binding pairs
    Patent number: 10508273
    Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: December 17, 2019
    Assignee: Inscripta, Inc.
    Inventors: Deanna Church, Stephen Federowicz, Michael Graige
  • Automated cell processing methods, modules, instruments, and systems comprising flow-through electroporation devices
    Patent number: 10508288
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments comprising one or more flow-through electroporation devices or modules are provided to automate genome editing in live cells.
    Type: Grant
    Filed: September 14, 2019
    Date of Patent: December 17, 2019
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Kevin Ness
  • Automated instrumentation for production of peptide libraries
    Patent number: 10501738
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of binding pairs. The engineered peptides may be displayed using various cell surface display technologies.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: December 10, 2019
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10478822
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: July 29, 2019
    Date of Patent: November 19, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10465185
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: May 28, 2019
    Date of Patent: November 5, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Methods for controlling the growth of prokaryotic and eukaryotic cells
    Patent number: 10443031
    Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.
    Type: Grant
    Filed: March 21, 2019
    Date of Patent: October 15, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader
  • Modification of cells by introduction of exogenous material
    Patent number: 10443074
    Abstract: The present disclosure provides methods and devices for rapid and efficient modification of a variety of cell types, including mammalian cells, plant cells, archaea, yeasts, and bacteria, by novel methods of introducing exogenous materials, e.g. nucleic acids.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: October 15, 2019
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier
  • Nucleic acid-guided nucleases
    Patent number: 10435714
    Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: October 8, 2019
    Assignee: INSCRIPTA, INC.
    Inventors: Ryan T. Gill, Andrew Garst, Tanya Elizabeth Warnecke Lipscomb
  • Automated control of cell growth rates for induction and transformation
    Patent number: 10435662
    Abstract: The present disclosure relates to methods and devices for automated control of cell growth rates where cell growth is measured in situ and the devices can be used as a stand-alone device or as a module in an automated environment, e.g., as one module in a multi-station or multi-module cell processing environment. The cell growth device comprises a temperature-controlled vial, a motor assembly to spin the vial, a spectrophotometer for measuring, e.g., OD of the cells in the vial, and a processor to accept input from a user and control the growth rate of the cells.
    Type: Grant
    Filed: March 21, 2019
    Date of Patent: October 8, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader
  • Flow through electroporation instrumentation
    Patent number: 10435713
    Abstract: The present disclosure provides a flow-through electroporation device configured for use as a stand-alone module or as one module in an automated multi-module processing system.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: October 8, 2019
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier