Patents Assigned to Inscripta, Inc.
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10639637
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: January 22, 2020
    Date of Patent: May 5, 2020
    Assignee: Inscripta, Inc.
    Inventors: Brian Van Hatten, Don Masquelier, Phillip Belgrader, Jorge Bernate, Bruce Chabansky
  • Engineered enzymes
    Patent number: 10640754
    Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.
    Type: Grant
    Filed: February 22, 2020
    Date of Patent: May 5, 2020
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Juhan Kim, Aamir Mir, Kyle Seamon, Andrew Garst
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10633627
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: October 9, 2019
    Date of Patent: April 28, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10633626
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: October 9, 2019
    Date of Patent: April 28, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10625212
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: April 21, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Andrew Garst, Richard Fox, Matthew Estes, Bruce Chabansky, Jorge Bernate
  • Nucleic acid-guided nucleases
    Patent number: 10626416
    Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.
    Type: Grant
    Filed: August 22, 2019
    Date of Patent: April 21, 2020
    Assignee: Inscripta, Inc.
    Inventors: Ryan T. Gill, Andrew Garst, Tanya Elizabeth Warnecke Lipscomb
  • Engineered enzymes
    Patent number: 10604746
    Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.
    Type: Grant
    Filed: October 21, 2019
    Date of Patent: March 31, 2020
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Juhan Kim, Aamir Mir, Kyle Seamon, Andrew Garst
  • Methods for controlling the growth of prokaryotic and eukaryotic cells
    Patent number: 10590375
    Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.
    Type: Grant
    Filed: August 27, 2019
    Date of Patent: March 17, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10584333
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: October 29, 2019
    Date of Patent: March 10, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10584334
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: March 10, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10576474
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: October 9, 2019
    Date of Patent: March 3, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Brian Van Hatten, Jorge Bernate, Bruce Chabansky
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10557216
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: February 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Automated nucleic acid assembly and introduction of nucleic acids into cells
    Patent number: 10557150
    Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.
    Type: Grant
    Filed: September 14, 2019
    Date of Patent: February 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10550363
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: February 4, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10532324
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: August 14, 2019
    Date of Patent: January 14, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Andrew Garst, Richard Fox, Matthew Estes, Bruce Chabansky
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10533152
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: January 14, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Bruce Chabansky, Jorge Bernate, Andrew Garst, Richard Fox
  • Methods for identifying T-cell receptor antigens
    Patent number: 10526598
    Abstract: The present disclosure automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptide antigens to identify potential TCR binding targets. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: January 7, 2020
    Assignee: Inscripta, Inc.
    Inventors: Deanna Church, Stephen Federowicz, Michael Graige
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10519437
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: September 14, 2019
    Date of Patent: December 31, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Automated cell processing methods, modules, instruments, and systems comprising flow-through electroporation devices
    Patent number: 10508288
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments comprising one or more flow-through electroporation devices or modules are provided to automate genome editing in live cells.
    Type: Grant
    Filed: September 14, 2019
    Date of Patent: December 17, 2019
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Kevin Ness
  • Methods for identifying selective binding pairs
    Patent number: 10508273
    Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.
    Type: Grant
    Filed: April 24, 2019
    Date of Patent: December 17, 2019
    Assignee: Inscripta, Inc.
    Inventors: Deanna Church, Stephen Federowicz, Michael Graige