Patents Assigned to Inscripta, Inc.
  • Genome-wide rationally-designed mutations leading to enhanced lysine production in
    Patent number: 10920189
    Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: February 16, 2021
    Assignee: Inscripta, Inc.
    Inventors: Richard Fox, Daniel Held, Eric Abbate, Michael Clay, Katherine Krouse, Nandini Krishnamurthy, Krishna Yerramsetty
  • Flow through electroporation modules and instrumentation
    Patent number: 10907125
    Abstract: The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.
    Type: Grant
    Filed: June 17, 2020
    Date of Patent: February 2, 2021
    Assignee: Inscripta, Inc.
    Inventors: Nathan Bade, Jorge Bernate, Phillip Belgrader, Don Masquelier
  • Automated cell processing methods, modules, instruments, and systems comprising flow-through electroporation devices
    Patent number: 10907178
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments comprising one or more flow-through electroporation devices or modules are provided to automate genome editing in live cells.
    Type: Grant
    Filed: May 30, 2019
    Date of Patent: February 2, 2021
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Kevin Ness
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10894958
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: August 9, 2020
    Date of Patent: January 19, 2021
    Assignee: Inscripta, Inc.
    Inventors: Kevin Ness, Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill
  • Methods for controlling the growth of prokaryotic and eukaryotic cells
    Patent number: 10883077
    Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.
    Type: Grant
    Filed: March 31, 2020
    Date of Patent: January 5, 2021
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier
  • Mad nucleases
    Patent number: 10883095
    Abstract: The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells.
    Type: Grant
    Filed: April 1, 2020
    Date of Patent: January 5, 2021
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Juhan Kim, Aamir Mir, Andrew Garst, Kyle Seamon
  • Engineered enzymes
    Patent number: 10876102
    Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.
    Type: Grant
    Filed: April 9, 2020
    Date of Patent: December 29, 2020
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Juhan Kim, Aamir Mir, Kyle Seamon, Andrew Garst
  • Nucleic acid-guided nucleases
    Patent number: 10870841
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: August 9, 2020
    Date of Patent: December 22, 2020
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Aamir Mir, Andrew Garst, Juhan Kim, Kyle Seamon
  • Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
    Patent number: 10858761
    Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.
    Type: Grant
    Filed: February 22, 2020
    Date of Patent: December 8, 2020
    Assignee: Inscripta, Inc.
    Inventors: Richard Fox, Daniel Held
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10851339
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: December 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Modification of cells by introduction of exogenous material
    Patent number: 10851389
    Abstract: The present disclosure provides methods and devices for rapid and efficient modification of a variety of cell types, including mammalian cells, plant cells, archaea, yeasts, and bacteria, by novel methods of introducing exogenous materials, e.g. nucleic acids.
    Type: Grant
    Filed: August 26, 2019
    Date of Patent: December 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10844344
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: March 16, 2020
    Date of Patent: November 24, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Curing for recursive nucleic acid-guided cell editing
    Patent number: 10837021
    Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.
    Type: Grant
    Filed: June 4, 2020
    Date of Patent: November 17, 2020
    Assignee: Inscripta, Inc.
    Inventors: Tian Tian, Charles Johnson, Eileen Spindler
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10835869
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: November 17, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Matthew Estes, Bruce Chabansky, Andrew Garst, Richard Fox, Jorge Bernate
  • Automated nucleic acid assembly and introduction of nucleic acids into cells
    Patent number: 10822621
    Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.
    Type: Grant
    Filed: August 22, 2019
    Date of Patent: November 3, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader
  • Simultaneous multiplex genome editing in yeast
    Patent number: 10815467
    Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: October 27, 2020
    Assignee: Inscripta, Inc.
    Inventors: Miles Gander, Tian Tian, Skylar Stefani
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10799868
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment. The reagent cartridges may include an electroporation device, as well as sample receptacles, reagent receptacles, waste receptacles and the like, and a script for controlling a processor to dispense samples and reagents contained in the receptacles, and to porate cells in the electroporation device. Also described are kits including the cartridges, automated multi-module cell processing instruments including the reagent cartridges and methods of using the reagent cartridges.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: October 13, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Brian Van Hatten, Bruce Chabansky
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10801008
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: July 15, 2020
    Date of Patent: October 13, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Electroporation cuvettes for automation
    Patent number: 10787683
    Abstract: The present disclosure relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment.
    Type: Grant
    Filed: July 13, 2020
    Date of Patent: September 29, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Phil Belgrader, Don Masquelier, Vlorent Morina
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10787663
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: July 6, 2020
    Date of Patent: September 29, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill, Kevin Ness