Patents Assigned to Inscripta, Inc.
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10851339
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: December 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Modification of cells by introduction of exogenous material
    Patent number: 10851389
    Abstract: The present disclosure provides methods and devices for rapid and efficient modification of a variety of cell types, including mammalian cells, plant cells, archaea, yeasts, and bacteria, by novel methods of introducing exogenous materials, e.g. nucleic acids.
    Type: Grant
    Filed: August 26, 2019
    Date of Patent: December 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10844344
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: March 16, 2020
    Date of Patent: November 24, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Curing for recursive nucleic acid-guided cell editing
    Patent number: 10837021
    Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.
    Type: Grant
    Filed: June 4, 2020
    Date of Patent: November 17, 2020
    Assignee: Inscripta, Inc.
    Inventors: Tian Tian, Charles Johnson, Eileen Spindler
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10835869
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: November 17, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Matthew Estes, Bruce Chabansky, Andrew Garst, Richard Fox, Jorge Bernate
  • Automated nucleic acid assembly and introduction of nucleic acids into cells
    Patent number: 10822621
    Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.
    Type: Grant
    Filed: August 22, 2019
    Date of Patent: November 3, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader
  • Simultaneous multiplex genome editing in yeast
    Patent number: 10815467
    Abstract: The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: October 27, 2020
    Assignee: Inscripta, Inc.
    Inventors: Miles Gander, Tian Tian, Skylar Stefani
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10801008
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: July 15, 2020
    Date of Patent: October 13, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10799868
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment. The reagent cartridges may include an electroporation device, as well as sample receptacles, reagent receptacles, waste receptacles and the like, and a script for controlling a processor to dispense samples and reagents contained in the receptacles, and to porate cells in the electroporation device. Also described are kits including the cartridges, automated multi-module cell processing instruments including the reagent cartridges and methods of using the reagent cartridges.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: October 13, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Brian Van Hatten, Bruce Chabansky
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10787663
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: July 6, 2020
    Date of Patent: September 29, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill, Kevin Ness
  • Electroporation cuvettes for automation
    Patent number: 10787683
    Abstract: The present disclosure relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment.
    Type: Grant
    Filed: July 13, 2020
    Date of Patent: September 29, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Phil Belgrader, Don Masquelier, Vlorent Morina
  • Automated instrumentation for production of peptide libraries
    Patent number: 10774324
    Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.
    Type: Grant
    Filed: October 31, 2019
    Date of Patent: September 15, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10774446
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: September 15, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Nucleic acid-guided nucleases
    Patent number: 10767169
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: September 8, 2020
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir, Andrew Garst, Kyle Seamon
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10760043
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: March 18, 2020
    Date of Patent: September 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10752874
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: November 15, 2019
    Date of Patent: August 25, 2020
    Assignee: Inscripta, Inc.
    Inventors: Charles Johnson, Miles Gander, Don Masquelier, Phillip Belgrader, Andrew Garst, Matthew Estes, Bruce Chabansky, Jorge Bernate
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10744463
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: April 9, 2020
    Date of Patent: August 18, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Matthew Estes, Bruce Chabansky, Andrew Garst, Richard Fox, Jorge Bernate
  • Nucleic acid-guided nucleases
    Patent number: 10745678
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: May 6, 2020
    Date of Patent: August 18, 2020
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir, Andrew Garst, Kyle Seamon
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10737271
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: August 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Brian Van Hatten, Don Masquelier, Phillip Belgrader, Jorge Bernate, Bruce Chabansky
  • Electroporation cuvettes for automation
    Patent number: 10738327
    Abstract: The present invention relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment. The electroporation device comprises an electroporation cuvette coupled with an adapter or engagement member at the top that is configured for engagement with liquid handling instrumentation, and a “sipper” conduit at the bottom for sample intake and output.
    Type: Grant
    Filed: August 22, 2018
    Date of Patent: August 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Phil Belgrader, Don Masquelier, Vlorent Morina