Patents Assigned to Inscripta, Inc.
  • Automated instrumentation for production of peptide libraries
    Patent number: 10774324
    Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.
    Type: Grant
    Filed: October 31, 2019
    Date of Patent: September 15, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10774446
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: September 15, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Nucleic acid-guided nucleases
    Patent number: 10767169
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: September 8, 2020
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir, Andrew Garst, Kyle Seamon
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10760043
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: March 18, 2020
    Date of Patent: September 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10752874
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: November 15, 2019
    Date of Patent: August 25, 2020
    Assignee: Inscripta, Inc.
    Inventors: Charles Johnson, Miles Gander, Don Masquelier, Phillip Belgrader, Andrew Garst, Matthew Estes, Bruce Chabansky, Jorge Bernate
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10744463
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: April 9, 2020
    Date of Patent: August 18, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Matthew Estes, Bruce Chabansky, Andrew Garst, Richard Fox, Jorge Bernate
  • Nucleic acid-guided nucleases
    Patent number: 10745678
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: May 6, 2020
    Date of Patent: August 18, 2020
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir, Andrew Garst, Kyle Seamon
  • Electroporation cuvettes for automation
    Patent number: 10738327
    Abstract: The present invention relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment. The electroporation device comprises an electroporation cuvette coupled with an adapter or engagement member at the top that is configured for engagement with liquid handling instrumentation, and a “sipper” conduit at the bottom for sample intake and output.
    Type: Grant
    Filed: August 22, 2018
    Date of Patent: August 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Phil Belgrader, Don Masquelier, Vlorent Morina
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10737271
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: August 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Brian Van Hatten, Don Masquelier, Phillip Belgrader, Jorge Bernate, Bruce Chabansky
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10738301
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: April 1, 2020
    Date of Patent: August 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill, Kevin Ness
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10723995
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: March 16, 2020
    Date of Patent: July 28, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Nucleic acid-guided nucleases
    Patent number: 10724021
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: May 6, 2020
    Date of Patent: July 28, 2020
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir, Andrew Garst, Kyle Seamon
  • Methods for controlling the growth of prokaryotic and eukaryotic cells
    Patent number: 10717959
    Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: July 21, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10711374
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: July 14, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Nucleic acid-guided nucleases
    Patent number: 10704033
    Abstract: The present disclosure provides novel RNA-guided enzymes for making rational and direct edits to the genome of live cells.
    Type: Grant
    Filed: December 13, 2019
    Date of Patent: July 7, 2020
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir, Andrew Garst, Kyle Seamon
  • Automated multi-module cell processing methods, instruments, and systems
    Patent number: 10689669
    Abstract: The present disclosure provides automated multi-module instruments, compositions and methods to increase the percentage of edited mammalian cells in a cell population when employing nucleic-acid guided editing.
    Type: Grant
    Filed: January 11, 2020
    Date of Patent: June 23, 2020
    Assignee: Inscripta, Inc.
    Inventors: Emily Feldman, Benjamin Mijts, Aamir Mir, Erik Zimmerman, Andrew Garst
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10689645
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: March 18, 2020
    Date of Patent: June 23, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill, Kevin Ness
  • Automated instrumentation for production of T-cell receptor peptide libraries
    Patent number: 10676842
    Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.
    Type: Grant
    Filed: October 31, 2019
    Date of Patent: June 9, 2020
    Assignee: Inscripta, Inc.
    Inventors: Stephen Federowicz, Deanna Church, Michael Graige
  • Engineered enzymes
    Patent number: 10655114
    Abstract: The present disclosure provides engineered RNA-guided enzymes for editing live cells.
    Type: Grant
    Filed: February 22, 2020
    Date of Patent: May 19, 2020
    Assignee: Inscripta, Inc.
    Inventors: Benjamin Mijts, Juhan Kim, Aamir Mir, Kyle Seamon, Andrew Garst
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10647982
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: January 23, 2020
    Date of Patent: May 12, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness