Patents Assigned to Institute Pasteur
  • Antigen-carrying microparticles and their use in the indication of humoral or cellular responses
    Patent number: 5871747
    Abstract: The invention concerns the use, in the induction of an immune response, of a synthetic microparticle polymer carrying on the surface one or more covalently bonded proteins capable of carrying one or more epitopes, the densities of the protein(s) on the surface of the microparticles, and their molecular weights, being adjusted so as to direct the immune response to the induction of a humoral and cellular response or to the induction of a largely cellular response. Said microparticles have an average diameter of approximately 0.25 to 1.5 .mu.m.
    Type: Grant
    Filed: April 28, 1995
    Date of Patent: February 16, 1999
    Assignee: Institut Pasteur
    Inventors: Christine Gengoux-Sedlik, Claude LeClerc
  • Nucleic acid probes, sequences and methods for detecting mycobacterium tuberculosis resistant to isoniazid
    Patent number: 5871912
    Abstract: Multi-drug resistant strains of Mycobacterium tuberculosis represent a considerable threat to public health worldwide. Resistance to isoniazid (INH), a key component of anti-tuberculosis regimens, is often associated with loss of catalase activity and virulence. The katG gene, encoding HPI catalase-peroxidase, mediates INH-sensitivity and that the high level resistance encountered clinically may be due to deletions, insertions or point mutations which reduce or eliminate the expression of the catalase gene in the chromosomal region encompassing katG. INH-resistant strains of Mycobacterium tuberculosis are detected by nucleic acid hybridization with a unique nucleic acid sequence or by amplification techniques.
    Type: Grant
    Filed: June 2, 1995
    Date of Patent: February 16, 1999
    Assignee: Institut Pasteur
    Inventors: Beate Heym, Stewart T. Cole, Douglas B. Young, Ying Zhang
  • Cytotoxic T lymphocyte-inducing lipopeptides and use as vaccines
    Patent number: 5871746
    Abstract: Cytotoxic lymphocyte-inducing lipopeptides comprising a peptide part having between 10 and 40 amino acids approximately and comprising at least one antigenic determinant. The lipopeptides also comprise one or more chains derived from fatty acids and one or more modified steroid groups.Said lipopeptides may be used for immunizing the human or animal body against pathogenic agents such as viruses or parasites. The peptide part may, in particular, be a fragment of the protein encoded by the ENV gene, by the NEF gene or by the GAG gene of HIV viruses. DA.
    Type: Grant
    Filed: May 31, 1994
    Date of Patent: February 16, 1999
    Assignees: Institut National de la Sainte et de la Recherche Medicale (INSERM), Institut Pasteur, Institut Pasteur De Lille
    Inventors: Christophe Boutillon, Frederic Martinon, Christian Sergheraert, Remy Magne, Helene Gras-Masse, Elisabeth Gomard, Andre Tartar, Jean-Paul Levy
  • Variant of LAV viruses
    Patent number: 5869631
    Abstract: A variant of a LAV virus, designated LAV.sub.ELI and capable of causing. AIDS. The cDNA and antigens of the LAV.sub.ELI virus can be used for the diagnosis of AIDS and pre-AIDS.
    Type: Grant
    Filed: February 19, 1991
    Date of Patent: February 9, 1999
    Assignee: Institut Pasteur
    Inventors: Marc Alizon, Pierre Sonigo, Simon Wain-Hobson, Luc Montagnier
  • Methods, kits, and probes for diagnosing HIV-2
    Patent number: 5866319
    Abstract: A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In one embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: February 2, 1999
    Assignee: Institut Pasteur
    Inventors: Marc Alizon, Luc Montagnier, Denise Guetard, Francois Clavel, Pierre Sonigo, Mireille Guyader
  • Mycobacterial proteins, microorganisms producing them and their use for vaccines and for the detection of tuberculosis
    Patent number: 5866130
    Abstract: Mycobacterium tuberculosis protein having a molecular weight of 20 779 Da, and hybrid proteins containing at least portions of its sequence. These proteins may in particular be used in vaccines or for the detection of specific tuberculosis antibodies.
    Type: Grant
    Filed: April 30, 1996
    Date of Patent: February 2, 1999
    Assignee: Institut Pasteur
    Inventors: Anne Laqueyrerie, Gilles Marchal, Pascale Pescher, Felix Romain
  • Nucleotide and peptide sequences of a hepatitis C virus isolate, diagnostic and therapeutic applications
    Patent number: 5866139
    Abstract: This invention relates to purified HCV E1 peptides, immunogenic composition comprising purified HCV E1 peptides, and a diagnostic kit for detecting HCV E1-specific antibodies. The purified HCV E1 peptide has an amino acid selected from the group consisting of SEQ ID NO:3; SEQ ID NO:5; and SEQ ID NO:7.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: February 2, 1999
    Assignee: Institut Pasteur
    Inventors: Christian Brechot, Dina Kremsdorf, Colette Porchon
  • Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
    Patent number: 5866361
    Abstract: An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: February 2, 1999
    Assignees: Institut Pasteur, Universite Pierre et Marie Curie
    Inventors: Bernard Dujon, Andre Choulika, Arnaud Perrin, Jean-Francois Nicolas
  • Bacterial diagnostic probe
    Patent number: 5863721
    Abstract: A test kit for the identification of various bacterial species groupings comprises a DNA probe which can identify palindromic units specific to particular bacterial species or species groupings. Such probes are highly specific for particular species or species subgroups. Accordingly, specific identification of bacteria may be achieved.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: January 26, 1999
    Assignee: Institut Pasteur
    Inventors: Eric Gilson, Jean-Marie Clement, David Perrin, Agnes Ullmann, Maurice Hofnung
  • Nucleotide sequences of human immunodeficiency virus type 2 (HIV-2), probes of HIV-2, and methods of using these probes
    Patent number: 5858651
    Abstract: A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In one embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.
    Type: Grant
    Filed: May 26, 1994
    Date of Patent: January 12, 1999
    Assignee: Institut Pasteur
    Inventors: Marc Alizon, Luc Montagnier, Denise Geutard, Francois Clavel, Pierre Sonigo, Mireille Guyader
  • Regulatory nucleotide sequence of the initiation of transcription
    Patent number: 5858773
    Abstract: The invention relates to a recombinant nucleotide sequence, characterized in that it comprises:a regulatory sequence of the initiation of transcription, this regulatory sequence containing a promoter in association with the motif GCACTC 9N GAGTGC, in which "N" signifies any one of the 4 bases thymine, guanine, adenine and cytosine;a sequence coding for a polypeptide, called "heterologous polypeptide", which is different from that naturally associated with the promoter; the coding sequence being positioned downstream from the regulatory sequence of the initiation of transcription at a site which, under suitable conditions, would allow the expression of the polypeptide under the control of the promoter.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: January 12, 1999
    Assignee: Institut Pasteur
    Inventors: Philippe Mazodier, Gerard Guglielmi
  • Peptides and polypeptides derived from the submaxillary gland of the rat, corresponding polyclonal and monoclonal antibodies, corresponding hybridomas and uses of these products for diagnosis, for detection or therapeutic purposes
    Patent number: 5859189
    Abstract: The present invention relates to peptides and polypeptides derived from the submaxillary gland of the rat. In particular, the present invention discloses a purified peptide of formula:X--His--Asn--Pro--Yin which X represents a Gln or Pro--Glu residue and Y represents an OH group or a residue of a basic amino acid. The present invention also relates to corresponding polyclonal and monoclonal antibodies as well as corresponding hybridomas. The products of the present invention are useful for therapeutic, diagnosis and detection purposes.
    Type: Grant
    Filed: November 17, 1993
    Date of Patent: January 12, 1999
    Assignee: Institut Pasteur
    Inventors: Isabelle Rosinski-Chupin, Diana Tronik, Francois Rougeon, Nabil Seidah
  • Constitutive pseudohyphal growth yeast mutants
    Patent number: 5858765
    Abstract: An isolated gene and mutations thereof capable of imparting constitutive pseudohyphal growth to S. cerevisiae is provided. The isolated wild type gene referred to as ELM1 is also capable of coding for a novel protein kinase that determines the yeast morphology and specific physiological properties.
    Type: Grant
    Filed: May 12, 1993
    Date of Patent: January 12, 1999
    Assignees: Iowa State University Research Foundation, Institut Pasteur, Institut National de la Sante et de la Recherche Medicale
    Inventors: Alan M. Myers, Pascal Madaule
  • Rapid detection of antibiotic resistance in mycobacterium tuberculosis
    Patent number: 5851763
    Abstract: A process for the detection of resistance to an antibiotic in a mycobacterium comprises detecting a mutation in a gene selected from the group consisting of the katG gene or fragment thereof, the rpoB gene or fragment thereof, and the rpsI gene or fragment thereof. The process is useful for detecting in vitro the presence of nucleic acids of a Mycobacterium tuberculosis resistant to isoniazid.
    Type: Grant
    Filed: October 12, 1994
    Date of Patent: December 22, 1998
    Assignee: Institut Pasteur
    Inventors: Beate Heym, Stewart Cole, Douglas Young, Ying Zhang, Nadine Honore, Amalio Telenti, Thomas Bodmer
  • Nucleotide sequences coding for a protein with urease activity
    Patent number: 5849295
    Abstract: The invention relates to a sequence of nucleotides, characterized in that it comprises at least a part of a sequence coding for a protein with urease activity such as that expressed by C. pylori.Another subject of the invention is the uses of this sequence, in particular for the in vitro diagnosis of diseases associated with the presence of Campylobacter pylori in the organism of an individual.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: December 15, 1998
    Assignees: Institut Pasteur, Institut National de la Sante et de la Recherche Medicale
    Inventor: Agnes Labigne
  • Highly specific surface for biological reactions having an exposed ethylenic double bond, process of using the surface, and method for assaying for a molecule using the surface
    Patent number: 5846724
    Abstract: The present invention relates especially to a highly specific surface for biological reactions, characterized in that it contains a support having at the surface at least one essentially compact layer of an organic compound having, outside the layer, an exposed group containing an ethylenic double bond having affinity for one type of molecule with biological activity under certain reaction conditions, the other elements of the layer being essentially inaccessible for the said molecules under the said reaction conditions.
    Type: Grant
    Filed: January 28, 1997
    Date of Patent: December 8, 1998
    Assignees: Institut Pasteur, Centre National de la Recherche Scientifique
    Inventors: David Bensimon, Aaron Bensimon, Fran.cedilla.ois Heslot
  • Immunogenic compositions against helicobacter infection, polypeptides for use in the compositions, and nucleic acid sequences encoding said polypeptides
    Patent number: 5843460
    Abstract: There is provided an immunogenic composition capable of inducing protective antibodies against Helicobacter infection characterized in that it comprises:i) at least one sub-unit of a urease structural polypeptide from Helicobacter pylori (SEQ ID NOS:22,26), or a fragment thereof, said fragment being recognized by antibodies reacting with Helicobacter felis urease (SEQ ID NOS:20-21), and/or at least one sub-unit of a urease structural polypeptide from Helicobacter felis (SEQ ID NOS:20-21), or a fragment thereof, said fragment being recognized by antibodies reacting with Helicobacter pylori urease (SEQ ID NOS:22-26);ii) and/or, a heat shock protein (Hsp), or chaperonin, from Helicobacter, or a fragment of said protein.The preparation, by recombinant means, of such immunogenic compositions is also provided.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: December 1, 1998
    Assignees: Institut Pasteur, Institut National de la Sante et de la Recherche Medicale
    Inventors: Agnes Labigne, Sebastin Suerbaum, Richard L. Ferrero, Jean-Michel Thiberge
  • Method for hypermutagenesis of nucleotides
    Patent number: 5843730
    Abstract: The present invention features a method for introducing hypermutations into a target DNA or RNA sequence of interest, characterized in that said method comprises the steps of:(a) transcribing a RNA into DNA in a reaction mixture comprising a reverse transcriptase, varying biased concentrations of deoxynucleoside triphosphates to produce hypermutations and an oligonucleotide primer that is partially complementary to the 3' end of said RNA; and(b) recovering said DNA sequences.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: December 1, 1998
    Assignee: Institut Pasteur
    Inventors: Simon Wain-Hobson, Miguel Angel Martinez, Valerie Pezo
  • Nucleic acids and pepties of human immunodeficiency virus type-1 (HIV-1).
    Patent number: 5843638
    Abstract: This invention is directed to nucleic acids derived from the pol region of the genome of human immunodeficiency virus type 1 (HIV-1). The nucleic acids are useful as probes for the detection of HIV-1. More particularly, this invention is directed to nucleic acids encoding a pol region of HIV-1 extending from about nucleotide 1856 to about 1906 and extending from about nucleotide 2048 to about nucleotide 2797.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: December 1, 1998
    Assignee: Institut Pasteur and Centre National de la Recherche Scientifique
    Inventors: Luc Montagnier, Bernard Krust, Solange Chamaret, Fran.cedilla.ois Clavel, Jean-Claude Chermann, Fran.cedilla.oise Barre-Sinoussi, Marc Alizon, Pierre Sonigo, Stewart Cole, Olivier Danos, Simon Wain-Hobson
  • Method of selection of allelic exchange mutants
    Patent number: 5843664
    Abstract: A process for replacing a nucleotide sequence in the genome of a mycobacterium strain comprises the steps of:a) providing a vector containing SacB gene coding for levane saccharase enzyme and a nucleotide sequence of interest;b) transfecting the mycobacterium strain with the vector;c) selecting clones of the resulting transfected mycobacteria for replacement of the nucleotide sequence of interest by propagating the transfected clones in a culture medium supplemented with sucrose; andd) isolating the recombinant strain.The process is useful for positive selection of allelic exchange mutants, such as in Mycobacterium tuberculosis complex.
    Type: Grant
    Filed: June 11, 1996
    Date of Patent: December 1, 1998
    Assignee: Institut Pasteur
    Inventors: Vladimir Pelicic, Jean-Marc Reyrat, Brigitte Gicquel