Patents Assigned to Institute Pasteur
  • Polynucleotides and their use for detecting enterococci and streptococci bacterial strains
    Patent number: 6054269
    Abstract: Polynucleotides and oligonucleotides for identification of species of the Streptococcus genus and the Enterococcus genus are provided. The polynucleotides and oligonucleotides are useful as probes and primers. Polypeptides expressed by the polynucleotides and oligonucleotides are useful for the preparation of monoclonal and polyclonal antibodies that recognize the polypeptides.
    Type: Grant
    Filed: June 25, 1997
    Date of Patent: April 25, 2000
    Assignee: Institut Pasteur
    Inventors: Fabien Garnier, Guy Gerbaud, Marc Galimand, Patrice Courvalin, Sylvie Dukta-Malen, Murielle Charles, Stefan Evers, Barbara Casadewall
  • Process for aligning macromolecules on a surface by passage through a meniscus
    Patent number: 6054327
    Abstract: Macromolecules such as nucleic acids, proteins, lipids or polysaccharides are aligned on a support surface by passing the macromolecules through a meniscus of a solvent containing the macromolecules. The meniscus may be that of a solvent between two surfaces at an interface of the solvent with air. One end of a macromolecule is attached to one surface which may be a glass surface and another end is free. The meniscus is moved relative to the surface to which the end is attached such as by evaporating the solvent or by moving the surface. As the macromolecule passes through the meniscus, the macromolecule aligns on the surface perpendicular to the meniscus. This method may be used in assaying, measuring intramolecular distance and/or separating of macromolecules.
    Type: Grant
    Filed: March 23, 1998
    Date of Patent: April 25, 2000
    Assignees: Institut Pasteur, Centre Nationale de la Recherche Scientifque
    Inventors: David Bensimon, Aaron Bensimon, Fran.cedilla.ois Heslot
  • Nucleic Acids of HIV-2, Diagnostic Test Kit and Method using Nucleic Acid Probes of HIV-2
    Patent number: 6054565
    Abstract: A method for diagnosing an HIV-2 (LAV-II) infection and a kit containing reagents for the same is disclosed. These reagents include cDNA probes which are capable of hybridizing to at least a portion of the genome of HIV-2. In the embodiment, the DNA probes are capable of hybridizing to the entire genome of HIV-2. These reagents also include polypeptides encoded by some of these DNA sequences.
    Type: Grant
    Filed: April 28, 1994
    Date of Patent: April 25, 2000
    Assignee: Institut Pasteur
    Inventors: Marc Alizon, Luc Montagnier, Denise Geutard, Francois Clavel, Pierre Sonigo, Mireille Guyader
  • Vectors for transforming CHO cells
    Patent number: 6051426
    Abstract: The invention concerns a composition useful for the manufacture of vaccines containing particles having the immunogenic properties characteristic of the antigen HBsAg, these particles being more particularly characterized by the fact that the particles equally contain a receptor for polymerized human albumin. They are obtained by transformation of human or animal cells by a vector containing a DNA sequence coding for the S and pre-S regions of a genome of viral hepatitis B, this DNA sequence being placed under the direct control of a promoter permitting the effective transcription of the sequence in the human or animal cells transformable by the vector.
    Type: Grant
    Filed: February 6, 1995
    Date of Patent: April 18, 2000
    Assignee: Institut Pasteur, Institut National de la Sante et de la Recherche Medicale and Centre National de la Recherche Scientifique
    Inventors: Elaine Sobczak, Yves Malpiece, deceased, Marie-Louise Michel, Pierre Tiollais, Rolf E. Streeck
  • In vitro diagnostic assay employing HIV-2 antigens for the detection of HIV-2 specific antibodies
    Patent number: 6048685
    Abstract: The molecular cloning and characterization of a novel human immunodeficiency virus type 2 (HIV-2), designated HIV-2.sub.ROD, is described. A recombinant .lambda. phage genomic library was screened with an HIV-2-specific probe to identify overlapping subgenomic clones. Fragments of these .lambda. phage clones were subcloned into a suitable vector to reconstitute the complete HIV-2.sub.ROD genome. The complete nucleotide sequence of this proviral clone was ascertained and the following genes and gene products identified: gag (including p16, p26, and p12), pol, vif, vpr, vpx, env, tat, rev, and nef. These gene products will be useful, inter alia, in in vitro diagnostic methods and kits for the detection of HIV-2-specific antisera.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: April 11, 2000
    Assignee: Institut Pasteur
    Inventors: Marc Alizon, Luc Montagnier, Denise Geutard, Francois Clavel, Pierre Sonigo, Mireille Guyader
  • Methods for the preparation of human immunodeficiency virus type 2 (HIV-2) and antigens encoped thereby
    Patent number: 6037165
    Abstract: Methods for producing the newly discovered HIV-2 retrovirus and its antigens are provided. The antigens are obtained from various HIV-2 retroviruses. The antigen compositions comprise a lysate of an HIV-2 retrovirus, a protein of an HIV-2 retrovirus, or a glycoprotein of an HIV-2 retrovirus. Specifically, the antigen composition can comprise p12, p16, p26, gp32, gp42, and gp140.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: March 14, 2000
    Assignee: Institut Pasteur
    Inventors: Luc Montagnier, Solange Chamaret, Denise Guetard, Marc Alizon, Fran.cedilla.ois Clavel, Mireille Guyader, Pierre Sonigo, Fran.cedilla.oise Brun-Vezinet, Marianne Rey, Christine Rouzioux, Christine Katlama
  • Bordetella pertussis and Bordetella parapertussis strains
    Patent number: 6030625
    Abstract: An immunogenic composition, characterized in that it comprises an adenyl cyclase-hemolysin (Ac-Hly) protein, or an immunogenic portion of this AC-Hly, of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, and in that it comprises, in addition, a bacterial extract containing the expression products of the vrg genes of a strain of Bordetella chosen from B. pertussis, B. parapertussis or B. bronchiseptica, or a portion of these expression products which is sufficient to induce an immune response in a host to which the extract might be administered.
    Type: Grant
    Filed: April 23, 1998
    Date of Patent: February 29, 2000
    Assignee: Institut Pasteur
    Inventors: Pascale Gueirard, Nicole Guiso
  • Genes of Helicobacter pylori necessary for the regulation and maturation of urease and their use
    Patent number: 6027878
    Abstract: Oligonucleotide sequences are disclosed specific to H. pylori urease and useful as DNA probes and primers in the detection of H. pylori infection in humans. Also disclosed are methods of hybridization and amplification using these sequences.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: February 22, 2000
    Assignees: Institut Pasteur, Inistitut National de la Sante et de la Recherche Medicale
    Inventors: Agnes Labigne, Valerie Cussac, Richard Ferrero
  • Antibodies directed against peptides derived from the SMR1 polypeptide
    Patent number: 6025143
    Abstract: The present invention relates to peptides and polypeptides derived from the submaxillary gland of the rat. In particular, the present invention discloses a purified peptide of formuls:X-His-Asn-Pro-Yin which X represents a Gln or Pro-Glu residue and Y represents an OH group or residue of a basic amino acid. The present invention also relates to corresponding polyclonal and monoclonal antibodies as well as corresponding hybridomas. The products of the present invention are useful for therapeutic, diagnosis and detection purposes.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: February 15, 2000
    Assignee: Institut Pasteur
    Inventors: Isabelle Rosinski-Chupin, Diana Tronik, Francois Rougeon, Nabil Seidah
  • Oligonucleotide sequences for the amplification of the genome of the retroviruses of the HIV-2 and SIV type, and their uses for in vitro diagnosis of the infections due to these viruses
    Patent number: 6020123
    Abstract: The invention relates to oligonucleotide sequences (or initiators) derived from the HIV-2 ROD virus genome and from that of the SIV-mac 142 virus, as well as their use in an in vitro method for the diagnosis of the infection of an individual by a HIV-2 type virus.
    Type: Grant
    Filed: November 18, 1994
    Date of Patent: February 1, 2000
    Assignees: Institut Pasteur, Institut National de la Sante et de la Recherche Medicale
    Inventors: Pierre Sonigo, Christian Brechot, Valerie Courgnaud
  • Plasmodium falciparum antigens inducing protective antibodies
    Patent number: 6017538
    Abstract: A molecule, protein or peptide characterized in that it is recognized by cytophilic antibodies from individuals who are immune to infection by Plasmodia, and recognized by non-cytophilic antibodies from individuals who are vulnerable to infection by Plasmodiae. The antibodies are capable of blocking the erythrocytic phase of the parasite by co-operating with accessory cells such as monocytes.
    Type: Grant
    Filed: August 8, 1995
    Date of Patent: January 25, 2000
    Assignee: Institut Pasteur
    Inventors: Pierre Druilhe, Hasnaq Bouharoun-Tayoun, Claude Oeuvray
  • Bacterial diagnostic probe
    Patent number: 6017711
    Abstract: A test kit for the identification of various bacterial species groupings comprises a DNA probe which can identify palindromic units specific to particular bacterial species or species groupings. Such probes are highly specific for particular species or species subgroups. Accordingly, specific identification of bacteria may be achieved.
    Type: Grant
    Filed: August 21, 1998
    Date of Patent: January 25, 2000
    Assignee: Institut Pasteur
    Inventors: Eric Gilson, Jean-Marie Clement, David Perrin, Agnes Ullmann, Maurice Hofnung
  • Cytotoxic T lymphocyte-inducing lipopeptides and use as vaccines
    Patent number: 6015564
    Abstract: Cytotoxic lymphocyte-inducing lipopeptides comprising a peptide part having between 10 and 40 amino acids approximately and comprising at least one tigenic determinant. The lipopeptides also comprise one or more chains derived from fatty acids and one or more modified steroid groups. Said lipopeptides may be used for immunizing the human or animal body against pathogenic agents such as viruses or parasites. The peptide part may, in particular, be a fragment of the protein encoded by the ENV gene, by the NEF gene or by the GAG gene of HIV viruses.
    Type: Grant
    Filed: February 10, 1999
    Date of Patent: January 18, 2000
    Assignees: Institut Pasteur de Lille, Institut National de la Sante et de la Recherche Medicale (INSERM)
    Inventors: Christophe Boutillon, Frederic Martinon, Christian Sergheraert, Remy Magne, Helene Gras-Masse, Elisabeth Gomard, Andre Tartar, Jean-Paul Levy
  • Polypeptides implicated in the expression of resistance to glycopeptides, in particular in gram-positive bacteria, nucleotide sequence coding for these polypeptides and use for diagnosis
    Patent number: 6013508
    Abstract: The invention relates to compositions and nucleic acids encoding polypeptides involved in the expression of resistance to glycopeptides, in particular to vancomycin and/or teicoplanin. The invention also relates to vectors containing said nucleic acids, transformed host cells and their use for the diagnosis of resistance to glycopeptides.
    Type: Grant
    Filed: November 28, 1997
    Date of Patent: January 11, 2000
    Assignee: Institut Pasteur
    Inventors: Michel Arthur, Sylvie Dukta-Malen, Catherine Molinas, Patrice Courvalin
  • Polypeptides and antibodies characteristic of papillomavirus, and diagnostic procedures and vaccines making use of them
    Patent number: 6010704
    Abstract: The present invention provides papillomavirus DNA, and more particularly DNA probes derived from papillomavirus. The present invention also provides procedures for the use of DNA derived from papillomavirus in the in vitro diagnosis of papillomavirus infections.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: January 4, 2000
    Assignee: Institut Pasteur and Institut National de la Sante et de la Recherche Medicale
    Inventors: Carol Ann Komly, Odile Croissant, Francoise Breitburd
  • Altered major histocompatibility complex (MHC) determinant and methods of using the determinant
    Patent number: 6011146
    Abstract: An altered MHC class I determinant comprises .alpha..sub.1, .alpha..sub.2, .alpha..sub.3, .beta..sub.2 -microglobulin (.beta..sub.2 m) polypeptide domains encoded by a mammalian MHC class I locus in which the .alpha..sub.3 domain is covalently linked to the .beta..sub.2 M domain. An altered MHC class II determinant comprises .alpha..sub.1, .alpha..sub.2, .beta..sub.1, and .beta..sub.2 polypeptide domains encoded by a mammalian MHC class II locus, in which the domains are covalently linked to form a polypeptide comprising the .beta..sub.2 -.alpha..sub.2 -.alpha..sub.1 -.beta..sub.1 domains in sequence. The altered MHC class I and class II determinants can be associated with an antigen to elicit an immune response. The invention can be used in the immunization or treatment of diseases such as AIDS, multiple sclerosis, lupus erythematosus, toxic shock or snake bite.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: January 4, 2000
    Assignees: Institut Pasteur, Institut Nationale de la Sante et de la Recherche Medicale
    Inventors: Estelle Mottez, Jean-Pierre Abastado, Philippe Kourilsky
  • Desaturase antigen of mycobacterium tuberculosis
    Patent number: 6010855
    Abstract: The present invention relates to the isolation of a new gene, des, which encodes a M. tuberculosis protein named DES. The des gene appears to be conserved among different Mycobacteria species. The amino acid sequence of the DES protein contains two sets of motifs that are characteristic of the active sites of enzymes from the class II diiron-oxo protein family. Among this family of proteins, DES shares significant homology with soluble stearoyl-ACP desaturases. DES is a highly antigenic protein, which is recognized by human sera from patients infected with M. tuberculosis and M. leprae but not by sera from tuberculous cattle. Thus, the DES protein provides a useful tool for the serodiagnostic analysis of tuberculosis.
    Type: Grant
    Filed: July 25, 1997
    Date of Patent: January 4, 2000
    Assignee: Institut Pasteur
    Inventors: Mary Jackson, Brigitte Gicquel
  • Antigen-carrying microparticles and their use in the induction of humoral or cellular responses
    Patent number: 6004763
    Abstract: The invention concerns the use, in the induction of an immune response, of a synthetic microparticle polymer carrying on the surface one or more covalently bonded proteins capable of carrying one or more epitopes, the molecular weight of the protein(s) on the surface of the microparticles, being adjusted so as to direct the immune response to the induction of a humoral and cellular response or to the induction of a mainly cellular response, said microparticles have an average diameter of approximately 0.25 to 1.5 .mu.m.
    Type: Grant
    Filed: May 12, 1998
    Date of Patent: December 21, 1999
    Assignee: Institut Pasteur
    Inventors: Christine Gengoux, Claude Leclerc
  • Mutated polynucleotide corresponding to a mutation responsible for prelingual non-syndromic deafness in the connexin 26 gene and method of detecting this hereditary defect
    Patent number: 5998147
    Abstract: A purified polynucleotide having a chain of nucleotides corresponding to a mutated sequence, which in a wild type form encodes a polypeptide implicated in hereditary sensory defect, wherein said mutated purified polynucleotide presents a mutation responsible for prelingual non-syndromic deafness selected from the group consisting of a specific deletion of at least one nucleotide.
    Type: Grant
    Filed: August 14, 1998
    Date of Patent: December 7, 1999
    Assignee: Institut Pasteur
    Inventors: Christine Petit, Francoise Denoyelle-Gryson, Dominique Weil, Sandrine Marlin-Duvernois, Jean-Luc Guesdon
  • Nucleotide sequence hybridizing specifically with a genomic nucleic sequence of campylobacter
    Patent number: 5998138
    Abstract: The present invention relates to a nucleic acid sequence isolated from C. coli which hybridizes specifically with the genomic nucleic acid of strains belonging to the species C. coli and which does not hybridize under the usual conditions with the nucleic acids of campylobacteria which do not belong to this species, nor with the genomic nucleic acids of bacteria related to the genus Campylobacter.
    Type: Grant
    Filed: July 1, 1997
    Date of Patent: December 7, 1999
    Assignee: Institut Pasteur
    Inventors: Veronique Stonnet, Jean-Luc Guesdon