Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: A transmitter sets a time length, determines a time point at which phases of frequency signals coincide within a range of the time length, defines a determined time point as a time reference point, aligns the phases of the frequency signals based on the time reference point, combines phase-aligned frequency, and transmits combined frequency signals to a receiver by the time length as the communication signals. The receiver receives the communication signals, extracts the phases of the frequency signals, obtains a time point at which the phases coincide, and determines an obtained time point as the time reference point.

Abstract: The invention provides a nondestructive inspection apparatus and nondestructive inspection method for inspecting the inside of a surface layer of a composite structure using cosmic-ray muons.

Abstract: A negative electrode used for a nonaqueous electrolyte solution battery having nonaqueous electrolyte solution containing lithium ion includes a metal carbon composite material. The metal carbon composite material has a porous carbon material having cavities, and a metal material made of metal to reversibly store or emit lithium ion. The metal material is arranged on a surface of the porous carbon material including inner surfaces of the cavities. The porous carbon material has a mass of 1-65 mass % when the metal carbon composite material is defined to have a mass of 100 mass %.

Abstract: [PROBLEMS] To provide an induction voltage control device (8) capable of accelerating arbitrary charged particles to an arbitrary energy level in synchronization with any magnetic excitation pattern even for an acceleration voltage (9a) of constant voltage by an induction acceleration cell (6) for acceleration and its control method. [MEANS FOR SOLVING PROBLEMS] An induction voltage control device (8) includes: a digital signal processing device (8d) for controlling a variable delay time according to a necessary variable delay time pattern obtained based on a magnetic excitation pattern, an equivalent acceleration voltage value pattern, and a passing signal (7a) of a bunch (3) from a bunch monitor (7); and a pattern generator (8b) for conversion to a gate signal pattern (8a) of a switching power source (5b). The induction voltage control device (8) controls a pulse density of the induction voltage (9) for acceleration per control unit.

Abstract: A reactor has a casing composed of a magnesia-based refractory, and a bottom plate composed of an MgO-graphite mixed refractory is disposed on a bottom part of this casing. A graphite crucible is provided at a bottom of the reactor, and the graphite crucible and the reactor are joined together by a magnesia cylinder. Iron ore powder, coal powder, and other such raw materials supplied into the reactor are irradiated with microwaves from microwave oscillators and are heated. The iron ore is reduced, and the resulting molten pig iron flows out through a hole into a crucible, and then is poured out of the crucible through another hole into a ladle. It is thereby possible to manufacture molten pig iron with high energy efficiency, instead of using blast-furnace iron-making.

Abstract: A pain sensory nerve stimulation apparatus includes: an electrode portion including: a first electrode, a tip end of which is adapted to be inserted into a skin; and at lease one second electrode which is disposed in a circumference of the first electrode without being electrically conductive with the first electrode, and which is adapted to be in contact with a skin; and a pulse signal supplier, supplying a pulse signal in which an electrical polarity of the first electrode is set as a anode and an electrical polarity of the second electrode is set as a cathode.

Abstract: A laser type ignition device for an internal combustion engine includes a laser oscillator mounted in the internal combustion engine so as to focus its laser beam on an air-fuel mixture supplied into the combustion chamber, a pressure sensor for detecting pressure in the combustion chamber, a power source for the laser oscillator and a control unit for controlling the power source to supply the laser oscillator with the current whose amount changes according to the pressure in the combustion chamber. The power of the laser beam increases as the pressure in the combustion chamber decreases to completely ignite the air-fuel mixture.

Abstract: It is an object of the present invention to provide an accelerator that can accelerate by itself all ions up to any energy level allowed by the magnetic fields for beam guiding, and provides an all-ion accelerator in which with trigger timing and a charging time of an induced voltage applied to an ion beam injected from a preinjector by induction cells for confinement and acceleration used in an induction synchrotron, digital signal processors for confinement and acceleration and pattern generators for confinement and acceleration generate gate signal patterns for confinement and acceleration on the basis of a passage signal of the ion beam and an induced voltage signal for indicating the value of the induced voltage applied to the ion beam, and intelligent control devices for confinement and acceleration perform feedback control of on/off of the induction cells for confinement and acceleration.

Abstract: The present invention provides a laser irradiation mass spectrometer capable of analyzing components of living tissue or living cells with high accuracy. It includes a laser unit for irradiating a sample with a beam of laser light and controlling the irradiation spot of the laser beam on the sample; and a mass analyzer for performing a mass analysis of the ions generated at the irradiation spot, where the mass analyzer uses a frequency-driven ion trap and a time-of-flight mass spectrometer to carry out the mass analysis. The ion trap of this system assuredly traps ions having large mass to charge ratios, and enables the system to carry out analyses on samples of large molecules. Preferably, a digital driving method is used to drive the aforementioned frequency-driven ion trap. Also, a multi-turn time-of-flight mass spectrometer may preferably be used as the aforementioned time-of-flight mass spectrometer.

Abstract: The present invention provides a laser irradiation mass spectrometer capable of analyzing components of living tissue or living cells with high accuracy. It includes a laser unit for irradiating a sample with a beam of laser light and controlling the irradiation spot of the laser beam on the sample; and a mass analyzer for performing a mass analysis of the ions generated at the irradiation spot, where the mass analyzer uses a frequency-driven ion trap and a time-of-flight mass spectrometer to carry out the mass analysis. The ion trap of this system assuredly traps ions having large mass to charge ratios, and enables the system to carry out analyses on samples of large molecules. Preferably, a digital driving method is used to drive the aforementioned frequency-driven ion trap. Also, a multi-turn time-of-flight mass spectrometer may preferably be used as the aforementioned time-of-flight mass spectrometer.

Abstract: A laser type ignition device for an internal combustion engine includes a laser oscillator mounted in the internal combustion engine so as to focus its laser beam on an air-fuel mixture supplied into the combustion chamber, a pressure sensor for detecting pressure in the combustion chamber, a power source for the laser oscillator and a control unit for controlling the power source to supply the laser oscillator with the current whose amount changes according to the pressure in the combustion chamber. The power of the laser beam increases as the pressure in the combustion chamber decreases to completely ignite the air-fuel mixture.

Abstract: An anhydrous chloride with a formula of MCl2 (M=Fe, Co or Ni) is dissolved into an anhydrous acetonitrile solvent to form a chloride-acetonitrile solution. Then, calcium carbide minute powders are added and dispersed in the chloride-acetonitrile solution at a molar quantity equal to or smaller by 1–30 mol % than the molar quantity of the anhydrous chloride to form a reactive solution. Then, the reactive solution is heated at a predetermined temperature to chemically react the anhydrous chloride with the calcium carbide minute powders in the reactive solution to form a transition metal acetylide compound having an M-C2-M bond, a tetragonal structure, and a formula of MC2 (herein, M=Fe, Co or Ni).

Abstract: A permanent magnet for a particle accelerator and a magnetic field generator, in which Nd—Fe—B based magnets are used but are not demagnetized so easily even when exposed to a radiation, are provided. A permanent magnet for a particle accelerator is used in an environment in which the magnet is exposed to a radiation at an absorbed dose of at least 3,000 Gy. The magnet includes R (which is at least one of the rare-earth elements), B, TM (which is at least one transition element and includes Fe) and inevitably contained impurity elements. The magnet is a sintered magnet that has been magnetized to a permeance coefficient of 0.5 or more and that has a coercivity HcJ of 1.6 MA/m or more.

Abstract: An anhydrous chloride with a formula of MCl2 (M?Fe, Co or Ni) is dissolved into an anhydrous acetonitrile solvent to form a chloride-acetonitrile solution. Then, calcium carbide minute powders are added and dispersed in the chloride-acetonitrile solution at a molar quantity equal to or smaller by 1-30 mol % than the molar quantity of the anhydrous chloride to form a reactive solution. Then, the reactive solution is heated at a predetermined temperature to chemically react the anhydrous chloride with the calcium carbide minute powders in the reactive solution to form a transition metal acetylide compound having an M—C2—M bond, a tetragonal structure, and a formula of MC2 (herein, M?Fe, Co or Ni).

Abstract: An anhydrous chloride with a formula of MCl2 (M=Fe, Co or Ni) is dissolved into an anhydrous acetonitrile solvent to form a chloride-acetonitrile solution. Then, calcium carbide minute powders are added and dispersed in the chloride-acetonitrile solution to form a reactive solution. Then, the reactive solution is thermally treated (first thermal treatment) to form a nano-powder made of a transition metal acetylide compound having an M-C2-M bond, a tetragonal structure, and a formula of MC2 (herein, M=Fe, Co or Ni). Then, the nano-powder is thermally treated (second thermal treatment) again at a temperature higher than the temperature in the first thermal treatment to form a carbon layer-covering transition metallic nano-structure wherein a metallic core made of the transition metal M is covered with a carbon layer.

Abstract: An atmosphere containing a hydrogen gas containing a hydrogen isotope, a water and a water vapor is disposed under a given condition of reduce pressure. Then, a process temperature for the atmosphere is controlled commensurate with the pressure of the atmosphere to control partial pressures of the hydrogen gas and the water vapor, and thus, control the separating performance of the hydrogen isotope from the hydrogen gas through a hydrogen-water chemical exchange reaction.