Patents Assigned to Koninklijke Nederlandse Akademie van Wetenschappen
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Patent number: 10947510Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising said epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of said culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in said culture medium.Type: GrantFiled: November 13, 2013Date of Patent: March 16, 2021Assignee: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Patent number: 10940180Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: GrantFiled: November 15, 2017Date of Patent: March 9, 2021Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Publication number: 20210047618Abstract: The invention relates to methods and media for differentiating cells, for example for obtaining enteroendocrine cells, and to uses of the cells and organoids obtained by said methods. The invention also relates to methods for modulating hormone expression in enteroendocrine cells and medical uses relating to such methods.Type: ApplicationFiled: June 20, 2017Publication date: February 18, 2021Applicant: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Johannes Carolus Clevers, Joep Beumer
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Publication number: 20210040454Abstract: The invention relates to differentiation methods for progenitor cells, e.g. mammalian epithelial stem cells, differentiation media for use in said methods, organoids and cells obtainable by said methods and uses, including therapeutic uses, thereof.Type: ApplicationFiled: March 1, 2017Publication date: February 11, 2021Applicant: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Johannes Carolus Clevers, Helmuth Gehart
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Patent number: 10883116Abstract: The invention relates to transduction compounds, buffers and methods for introducing molecules into cells. The invention also relates to methods of treatment, pharmaceutical compositions and other uses of the transduction compounds and buffers. The invention also relates to modified cells obtainable by the transduction compounds, buffers and methods of the invention.Type: GrantFiled: August 28, 2014Date of Patent: January 5, 2021Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Niels Geijsen, Diego Sebastián D'Astolfo
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Publication number: 20200318063Abstract: The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.Type: ApplicationFiled: June 19, 2020Publication date: October 8, 2020Applicant: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Meritxell Huch Ortega, Johannes Carolus Clevers, Sylvia Fernandez Boj
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Publication number: 20200172861Abstract: The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.Type: ApplicationFiled: January 15, 2020Publication date: June 4, 2020Applicant: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Meritxell Huch Ortega, Johannes Carolus Clevers, Sylvia Fernandez Boj
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Patent number: 10597633Abstract: The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.Type: GrantFiled: May 15, 2015Date of Patent: March 24, 2020Assignee: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Meritxell Huch Ortega, Johannes Carolus Clevers, Sylvia Fernandez Boj
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Patent number: 10583149Abstract: The invention relates to the diagnostic and therapeutic uses of a miRNA molecule, an equivalent or a source thereof in a disease and condition associated with melanoma or a disease or a condition associated with activated BRAF pathway.Type: GrantFiled: June 16, 2017Date of Patent: March 10, 2020Assignees: INTERA TECHNOLOGIES B.V., KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPENInventors: Eugene Berezikov, Jos Bernard Poell, Willemijn Maria Gommans, Rick Jan van Haastert, Andreas Alphons F. L. van Puijenbroek, Roeland Quirinus Jozef Schaapveld, Gregoire Pierre Andre Prevost
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Publication number: 20190383799Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: ApplicationFiled: June 8, 2018Publication date: December 19, 2019Applicants: UMC Utrecht Holding B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Patent number: 10508095Abstract: Fermentation processes that use fungi of the genus Ulocladium for producing novel compounds that include an 8- or 9-membered cyclic structure, wherein an (E)-1,4-dioxo-but-2-ene moiety is embedded in the cyclic structure. The compounds may be used as medicaments, in particular as medicaments for treating and preventing microbial infections, such as infections by multidrug resistant and/or Gram-positive bacteria.Type: GrantFiled: February 1, 2018Date of Patent: December 17, 2019Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeroen den Hertog, Gisela Johanna van der Velden, Jelmer Hoeksma
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Publication number: 20190100728Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in the culture medium.Type: ApplicationFiled: August 27, 2018Publication date: April 4, 2019Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Publication number: 20190040033Abstract: The present invention relates to novel compounds, comprising an 8- or 9-membered cyclic structure, wherein an (E)-1,4-dioxo-but-2-ene moiety is embedded in said cyclic structure and to pharmaceutical compositions comprising these compounds. The compounds may be used as medicaments, in particular as medicaments for treating and preventing microbial infections, such as infections by multidrug resistant and/or Gram-positive bacteria. The invention further relates to fermentation processes wherein fungi of the genus Ulocladium are used for producing the novel compounds.Type: ApplicationFiled: February 1, 2018Publication date: February 7, 2019Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeroen den Hertog, Gisela Johanna van der Velden, Jelmer Hoeksma
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Publication number: 20180282799Abstract: The current invention relates to strategies for selection and amplification of genomic regions of interest. The strategy involves an amplification step in a host cell. A target nucleotide sequence associated with the genomic region of interest is used to selectively provide a DNA circles derived from the genomic region of interest with an origin of replication and a selection gene. This is in particular useful i.a. for determining DNA sequences of a genomic region of interest, for use in building contiguous sequences and/or for DNA mapping.Type: ApplicationFiled: October 5, 2016Publication date: October 4, 2018Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Wouter Leonard DE LAAT, Carlo VERMEULEN
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Publication number: 20180221441Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: ApplicationFiled: November 15, 2017Publication date: August 9, 2018Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Patent number: 10006904Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: GrantFiled: December 19, 2012Date of Patent: June 26, 2018Assignees: UMC Utrecht Holding B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Patent number: 9920025Abstract: The present invention relates to novel compounds, comprising an 8- or 9-membered cyclic structure, wherein an (E)-1,4-dioxo-but-2-ene moiety is embedded in said cyclic structure and to pharmaceutical compositions comprising these compounds. The compounds may be used as medicaments, in particular as medicaments for treating and preventing microbial infections, such as infections by multidrug resistant and/or Gram-positive bacteria. The invention further relates to fermentation processes wherein fungi of the genus Ulocladium are used for producing the novel compounds.Type: GrantFiled: July 30, 2015Date of Patent: March 20, 2018Assignee: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Jeroen Den Hertog, Gisela Johanna Van Der Velden, Jelmer Hoeksma
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Publication number: 20180072995Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids, pancreatic organoids, liver organoids, colon organoids, Barrett's Esophagus organoids, adenocarcinoma organoids and colon carcinoma organoids that are formed in the culture medium.Type: ApplicationFiled: July 19, 2017Publication date: March 15, 2018Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Publication number: 20180066233Abstract: The invention relates to a liver organoid, uses thereof and method for obtaining them.Type: ApplicationFiled: July 31, 2017Publication date: March 8, 2018Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Meritxell Huch Ortega, Johannes Carolus
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Patent number: 9833496Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: GrantFiled: September 23, 2014Date of Patent: December 5, 2017Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering