Abstract: The invention relates to the diagnostic and therapeutic uses of a miRNA molecule, an equivalent or a source thereof in a disease and condition associated with melanoma or a disease or a condition associated with activated BRAF pathway.
Type:
Application
Filed:
January 4, 2013
Publication date:
May 2, 2013
Applicants:
Koninklijke Nederlandse Akademie van Wetenschappen, InteRNA Technologies B.V.
Inventors:
InteRNA Technologies B.V., Koninklijke Nederlandse Akademie van Wetensch
Abstract: The invention provides a method for characterising a sample comprising nucleic acid derived from a cell. The method comprises determining whether a sample comprises at least a minimal sequence of at least one new microRNA (miRNA) according to the invention or a mammalian ortholog thereof and characterizing the sample on the basis of the presence or absence of the miRNA. The invention further provides nucleic acid molecules and collections thereof and their use in therapeutic and diagnostic applications. The invention furthermore provides a method for identifying a miRNA molecule or a precursor molecule thereof.
Type:
Grant
Filed:
January 10, 2007
Date of Patent:
January 29, 2013
Assignee:
Koninklijke Nederlandse Akademie van Wetenschappen
Inventors:
Eugene Berezikov, Edwin P. J. G. Cuppen
Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in the culture medium.
Type:
Application
Filed:
February 3, 2010
Publication date:
February 2, 2012
Applicant:
Koninklijke Nederlandse Akademie van Wetenschappen
Inventors:
Toshiro Sato, Johannes Carolus Clevers, Meritxell Huch Ortega
Abstract: The invention relates to a method for testing whether a compound is capable of inhibiting the development of lymphatic channels or lymphangiogenesis and/or the migration of lymphangioblasts in an non-human animal, a non-human embryo or a cell culture, comprising steps of contacting a compound capable of interacting with a Ccbe1 gene, a transcript thereof or a ccbe1 protein with a non-human animal, a non-human embryo or a cell culture; determining whether said compound inhibits the development of lymphatic channels, lymphangiogenesis and/or migration of lymphangioblasts in said non-human animal, a non-human embryo or a cell culture. The invention further related to a method of determining whether an individual is a carrier of, or is suffering from, or at risk of suffering from, a lymph vessel disorder, and to a medicament comprising Ccbe1, or comprising a nucleic acid encoding Ccbe1, for the treatment of a lymph vessel disorder.
Type:
Application
Filed:
May 17, 2011
Publication date:
November 24, 2011
Applicant:
Koninklijke Nederlandse Akademie van Wetenschappen
Inventors:
Stefan Schulte-Merker, Benjamin M. Hogan
Abstract: Provided is a method for characterising a sample comprising nucleic acid derived from a cell. The method comprises determining whether a sample comprises at least a minimal sequence of at least one new microRNA (miRNA) as disclosed herein or a mammalian ortholog thereof and characterizing the sample on the basis of the presence or absence of the miRNA. The invention further provides new nucleic acid molecules and collections thereof and their use in therapeutic and diagnostic applications. The invention furthermore provides a method for identifying a miRNA molecule or a precursor molecule thereof.
Type:
Application
Filed:
December 23, 2010
Publication date:
June 9, 2011
Applicant:
Koninklijke Nederlandse Akademie van Wetenschappen
Inventors:
Ronald H.A. Plasterk, Eugene Berezikov, Edwin P.J.G. Cuppen
Abstract: Method of an arrangement for monitoring sleep in a subject by measuring within a prescribed interval skin temperature of a predetermined region of the subject's body and a motion sensor for sensing motion of the subject, comparing the measured skin temperature of the predetermined region with a predetermined temperature threshold, and classifying the subject as being asleep or awake based on whether the skin temperature of the predetermined region is above or below the temperature threshold and on the motion data. In alternative aspects the invention relates to methods of and arrangements for manipulating sleep, as well as monitoring or manipulating alertness.
Type:
Application
Filed:
December 15, 2008
Publication date:
April 22, 2010
Applicant:
Koninklijke Nederlandse Akademie van Wetenschappen
Abstract: The present invention provides a method and device of measuring the retinal stray light in the eye of a subject, comprising presenting to the subject a flickering stray light from a stray light source; presenting to the subject compensation light so as to undercompensate said part of the flickering stray; presenting to the subject compensation light, the compensation light flickering with a modulation depth so as to eventually overcompensate the flickering light being imaged on the retina; and determining from the results of a sequence of comparisons using different parameter values between the resulting retinal light flickering as perceived by the subject for the undercompensated test area and the eventually overcompensated test area a stray light parameter.
Type:
Grant
Filed:
August 12, 2004
Date of Patent:
March 9, 2010
Assignee:
Koninklijke Nederlandse Akademie van Wetenschappen
Inventors:
Thomas Joannes Theodorus Paschalis Van den Berg, Joris Eduard Coppens