Patents Assigned to Koninklijke Nederlandse Akademie van Wetenschappen
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Publication number: 20170342385Abstract: The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.Type: ApplicationFiled: November 27, 2015Publication date: November 30, 2017Applicant: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Lars Norman Sachs, Ana Gracanin, Jarno Drost
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Patent number: 9822336Abstract: A method for making an at least double layered cell aggregate and/or an artificial blastocyst, and/or a further-developed blastoid termed blastoid, by forming a double layered cell aggregate from at least one trophoblast cell and at least one pluripotent and/or totipotent cell, and culturing the aggregate to obtain an artificial blastocyst having a trophectoderm-like tissue that surrounds a blastocoel and an inner cell mass-like tissue. The cell aggregate can be formed from toti- or pluripotent stem cell types, or induced pluripotent stem cell types, in combination with trophoblast stem cells. Formation of a blastoid can be achieved by culturing the cell aggregate in a medium preferably comprising one or more of a Rho/ROCK inhibitor, a Wnt pathway modulator, a PKA pathway modulator, a PKC pathway modulator, a MAPK pathway modulator, a STAT pathway modulator, an Akt pathway modulator, a Tgf pathway modulator and a Hippo pathway modulator.Type: GrantFiled: April 16, 2014Date of Patent: November 21, 2017Assignees: UNIVERSITEIT MAASTRICHT, KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPENInventors: Nicolas Clément Rivron, Clemens Antoni Van Blitterswijk, Niels Geijsen, Erik Jacob Vrij
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Publication number: 20170304348Abstract: The invention relates to the diagnostic and therapeutic uses of a miRNA molecule, an equivalent or a source thereof in a disease and condition associated with melanoma or a disease or a condition associated with activated BRAF pathway.Type: ApplicationFiled: June 16, 2017Publication date: October 26, 2017Applicants: INTERNA TECHNOLOGIES B.V., KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPENInventors: Eugene Berezikov, Jos Bernard Poell, Willemijn Maria Gommans, Rick Jan van Haastert, Andreas Alphons F.L. van Puijenbroek, Roeland Quirinus Jozef Schaapveld, Gregoire Pierre Andre Prevost
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Publication number: 20170275592Abstract: The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.Type: ApplicationFiled: November 27, 2015Publication date: September 28, 2017Applicant: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Lars Norman SACHS, Jarno DROST
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Patent number: 9765301Abstract: The invention relates to a liver organoid, uses thereof and method for obtaining them.Type: GrantFiled: July 29, 2011Date of Patent: September 19, 2017Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Meritxell Huch Ortega, Johannes Carolus Clevers
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Patent number: 9752124Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids, pancreatic organoids, liver organoids, colon organoids, Barrett's Esophagus organoids, adenocarcinoma organoids and colon carcinoma organoids that are formed in the culture medium.Type: GrantFiled: July 29, 2011Date of Patent: September 5, 2017Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Publication number: 20170210722Abstract: The present invention relates to novel compounds, comprising an 8-or 9-membered cyclic structure, wherein an (E)-1,4-dioxo-but-2-ene moiety is embedded in said cyclic structure and to pharmaceutical compositions comprising these compounds. The compounds may be used as medicaments, in particular as medicaments for treating and preventing microbial infections, such as infections by multidrug resistant and/or Gram-positive bacteria. The invention further relates to fermentation processes wherein fungi of the genus Ulocladium are used for producing the novel compounds.Type: ApplicationFiled: July 30, 2015Publication date: July 27, 2017Applicant: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Jeroen DEN HERTOG, Gisela Johanna VAN DER VELDEN, Jelmer HOEKSMA
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Publication number: 20170191030Abstract: The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.Type: ApplicationFiled: May 15, 2015Publication date: July 6, 2017Applicant: KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPENInventors: Meritxell HUCH ORTEGA, Johannes Carolus CLEVERS, Sylvia FERNANDEZ BOJ
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Publication number: 20170151308Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: ApplicationFiled: September 23, 2014Publication date: June 1, 2017Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Publication number: 20160273001Abstract: The invention relates to transduction compounds, buffers and methods for introducing molecules into cells. The invention also relates to methods of treatment, pharmaceutical compositions and other uses of the transduction compounds and buffers. The invention also relates to modified cells obtainable by the transduction compounds, buffers and methods of the invention.Type: ApplicationFiled: August 28, 2014Publication date: September 22, 2016Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Niels Geijsen, Diego Sebastián D'Astolfo
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Publication number: 20150276719Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: ApplicationFiled: December 19, 2012Publication date: October 1, 2015Applicants: UMC Utrecht Holding B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Publication number: 20150231201Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: ApplicationFiled: September 23, 2014Publication date: August 20, 2015Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Publication number: 20150011420Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: ApplicationFiled: December 19, 2012Publication date: January 8, 2015Applicants: UMC Utrecht Holding B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Patent number: 8906631Abstract: Described are the use of stem cell markers for the isolation of stem cells and the use of the obtained stem cells in, for example, research or treatment, such as for the preparation of a medicament for the treatment of damaged or diseased tissue. In one aspect, provided is a method for obtaining or isolating stem cells that comprises preparing a cell suspension from a tissue or organ sample, contacting the cell suspension with an Lgr 6 or 5 binding compound, identifying the cells bound to the binding compound, and isolating the stem cells from the binding compound. Further described are methods and compositions for cancer treatment, for example, by eradicating cancer stem cells.Type: GrantFiled: May 10, 2010Date of Patent: December 9, 2014Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes Carolus Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Patent number: 8895720Abstract: The invention provides a method for characterising a sample comprising nucleic acid derived from a cell. The method comprises determining whether a sample comprises at least a minimal sequence of at least one new microRNA (miRNA) according to the invention or a mammalian ortholog thereof and characterizing the sample on the basis of the presence or absence of the miRNA. The invention further provides nucleic acid molecules and collections thereof and their use in therapeutic and diagnostic applications. The invention furthermore provides a method for identifying a miRNA molecule or a precursor molecule thereof.Type: GrantFiled: December 20, 2012Date of Patent: November 25, 2014Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Ronald H. A. Plasterk, Eugene Berezikov, Edwin Pieter Cuppen
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Publication number: 20140256037Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising said epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of said culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in said culture medium.Type: ApplicationFiled: November 13, 2013Publication date: September 11, 2014Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Publication number: 20140243227Abstract: Culture media and methods for expanding and differentiating populations of stem cells and for obtaining organoids. Expanded cell populations and organoids obtainable by methods of the invention and their use in drug screening, toxicity assays and regenerative medicine.Type: ApplicationFiled: June 11, 2012Publication date: August 28, 2014Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Toshiro Sato, Meritxell Huch Ortega, Wouter Richard
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Publication number: 20140044713Abstract: The present invention relates to compounds that act as agonists of the Wnt signalling pathway, compositions comprising these compounds and the uses of these compounds, both therapeutic and in research. The invention also provides methods of identifying compounds that act as agonists of the Wnt signalling pathway.Type: ApplicationFiled: April 16, 2012Publication date: February 13, 2014Applicant: KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPENInventors: Willibrordus Barend Maria De Lau, Johannes Carolus Clevers
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Patent number: 8642339Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in the culture medium.Type: GrantFiled: February 3, 2010Date of Patent: February 4, 2014Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes Carolus Clevers
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Publication number: 20130178387Abstract: The invention provides a method for characterising a sample comprising nucleic acid derived from a cell. The method comprises determining whether a sample comprises at least a minimal sequence of at least one new microRNA (miRNA) according to the invention or a mammalian ortholog thereof and characterizing the sample on the basis of the presence or absence of the miRNA. The invention further provides nucleic acid molecules and collections thereof and their use in therapeutic and diagnostic applications. The invention furthermore provides a method for identifying a miRNA molecule or a precursor molecule thereof.Type: ApplicationFiled: December 20, 2012Publication date: July 11, 2013Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventor: Koninklijke Nederlandse Akademie van Wetenschappen