Abstract: The present invention relates to a fructosyl amino acid oxidase that is obtainable from a culture of a strain of the genus Aspergillus. This strain grows in a selective medium containing at least either of fructosyl lysine and fructosyl-N.sup..alpha. -Z-lysine and is capable of producing a fructosyl amino acid oxidase.

Abstract: A method for fixing a reagent layer directly onto a supporting base plate in the preparation of a dry analysis kit for determining a specific component in a liquid specimen wherein at least one of the reagent layer and the base plate is a thermoplastic resin, and which comprises the steps of:placing the reagent layer in contact with the base plate and externally applying ultrasonic vibration and pressure to the two layers to generate frictional heat thereby to melt the surface of the thermoplastic resin;applying pressure to make the molten surface of thermoplastic resin bite the non-thermoplastic material or to integrate the surfaces of thermoplastic layer and base plate; andremoving the ultrasonic vibration and pressure.

Abstract: In a non-collinear type acousto-optic tunable filter, a source light beam is made off-perpendicularly incident on a crystal body, so that the cross section of the source light beam is narrowed within the crystal body. As a result, the receiving angular aperture becomes large to increase the amount of light collected into the crystal body. Consequently, highly accurate spectrometry can be performed even if the intensity of the source light beam is low. Further, the non-diffraction part of the crystal body can be eliminated by the off-perpendicular incidence of the source light beam, so that the sufficient diffraction length of acoustic and optic waves can be obtained.

Abstract: This invention provides a device for analyzing a sample which is capable of performing rapid and precise analysis of a small amount of sample, and whose gradient in a measuring apparatus is not limited. This device comprises approximately a rectangular plate shaped body comprising a base member made of resin and a transparent covering. A first depressed cylindrical concave portion is formed in the upper surface of the base member, and a groove is formed in communication with the first depressed cylindrical concave portion, the groove extending to the end of the protrusion portion 5c, and a second depressed cylindrical concave portion which is smaller than the first depressed cylindrical concave portion is formed in a certain position in the groove, the end of the groove opening to the outside at the end of the protrusion portion 5c. Then, the transparent covering is placed over the upper surface of the base member and then integrated together.

Abstract: A method for measuring the amount of glycosylated protein comprising the steps of decomposing glycosylated proteins with protease, causing a redox reaction between the decomposed products and an Amadori compound oxidoreductase, and determining the redox reaction so as to measure the amount of glycosylated proteins, wherein the decomposition with the protease is carried out in the presence of at least one substrate selected from the group consisting of metalloporphyrin, cytochrome and diaphorase.

Abstract: A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.

Abstract: In a non-collinear type acousto-optic tunable filter, the incident angle of a source light beam L.sub.1 radiated from a light source 6 onto an acoustic medium 1 is set at an equivalence incident angle for which the wavelength .lambda..sub.i of the diffracted ordinary ray L.sub.3 and the wavelength .lambda..sub.i ' of the diffracted extraordinary ray L.sub.4 become approximately identical. Further, the diffracted ordinary ray L.sub.3 and the diffracted extraordinary ray L.sub.4 of the approximately identical wavelength are superposed, and the intensity of the superposed ray is detected. Consequently, spectrometry is performed based on the superposed diffracted ray having twice the intensity and a very sharp waveform, so that accurate spectroscopy can be made possible even if the intensity of the source light beam is low.

Abstract: A dry test piece for measuring a magnesium concentration in a biological fluid, which comprises the following reagent components (i) to (iii): (i) o-cresolphthalein complexon; (ii) O,O'-bis(2-aminophenyl)ethylene glycol-N,N,N',N'-tetraacetic acid; and (iii) a pH buffer agent of pH 8.5 to 11.0.

Abstract: A reagent composition for detecting an analyte in a liquid sample, comprising an iron (III) complex of a tetrakis (N-hydroxyalkyl) ethylenediamine represented by the general formula (1): ##STR1## wherein n, m, p, and q are each independently integers of 1 to 3.

Abstract: A device for assay of a liquid sample. The device comprises: a support composed of an organic macromolecule, the support having a surface divided into two areas located adjacent to each other; a divider in the surface, defining the border of both areas to separate a first area from a second area; a detection layer affixed to the first area and containing a reagent; and a water-swelling layer affixed to the second area, the water-swelling layer expanding by absorbing water.

Abstract: A light equipment irradiating a target with light adjusts light from a light source in a light source optical system and introduces the same into an acousto-optic device, for separating the same into its spectral components and modulating the same in the acousto-optic device. A condensing optical system condenses first order diffractive light outgoing from the acousto-optic device, so that an irradiation optical system irradiates a target with the same. A photodetection device comprises a photoreceiving part and a data processing part, so that the photoreceiving part converts a signal responsive to the fluctuation of modulated measuring light to a plurality of signals of different degrees of amplification simultaneously outputting same. The data processing part selects a non-saturated value of an amplifier or an A-D convertor while maintaining the largest degree of amplification from these signals, and lock-in processes same with a modulation frequency modulating the measuring light.

Abstract: The present invention provides a recombinant protein which shows fructosyl amino acid oxidase activity, a DNA encoding the same, an expression vector containing the DNA, a transformant transformed by the expression vector, and the method of preparing recombinant fructosyl amino acid oxidase by culturing the resultant transformant, and the recombinant fructosyl amino acid oxidase thus obtained.

Abstract: A process which makes possible to produce a glycosylated amino compound (e.g. protein) easily in short period without denaturation of protein. The sugar (e.g. glucose) and protein (e.g. serum) are mixed, and the mixture is dried under reduced pressure, so that sugar and protein quickly react with each other to produce the glycosylated protein. The drying under reduced pressure is preferably lyophilization which is conducted under the following conditions: the temperature is about -20.degree. C., the pressure is about 2 mmHg, and the treatment time is 12 hours. Unreacted glucose is then removed by the dialysis process, thus providing the required glycosylated protein sterilization need not be carried out. This process makes it possible to produce a glycosylated protein in about one day as compared to 7 days by the conventional process, so that the denaturation of protein is less likely to occur.

Abstract: An optical analyzer includes a light emitting diode (LED). The LED has a body pervious to light and a PN junction semiconductor. The body has a surface portion where a primary light emitted from semiconductor is divided into a first light and a second light. The first light is reflected at a sample and then received by a first detector, while the second light is detected by a second detector. An optical property of the sample is determined by the use of outputs of the first and second detectors.

Abstract: To secure a highly accurate measurement value of biological information with minimized variation by positioning a target part of a living body with good reproducibility and by positioning the target part of the living body easily with high reproducibility without pressing blood vessels in the living body. The concentration of a particular component in the living body is measured by the use of a transmitted or reflected spectrum obtained by projecting light onto the target part of the living body. A biological information measuring template 2 includes a shape memory medium 6 having a contact surface which, when the target part is pressed to the contact surface, undergoes change according to the shape of the target part, to thereby store the shape. The target part 1 of the living body is arranged at a store portion of the shape memory medium and is illuminated by light.

Abstract: A simple device for holding liquid for an analysis of a liquid sample, said device comprising a material composed of organic macromolecule, having a surface divided into a plurality of areas which posses contact angles with regard to liquid that are different from each other, one of which is surrounded with the other. With regard to the analysis of test liquids, the device enables the easy holding of even minute quantities of liquid and offers sufficient quantitative precision for analysis.

Abstract: A method for avoiding influence of hemoglobin, in which the influence is caused by the changes with time in the absorption wavelength of hemoglobin when light absorption of a color reaction of a sample is measured by a rate assay, wherein the method comprises measuring the light absorption at a wavelength of from 517 to 529 nm or from 580 to 592 nm.

Abstract: This invention provides a method for measuring the concentration of urinary trypsin inhibitors which is excellent in precision and reproducibility, whose operation is simple, and in which a possibility of damaging a plastic cell is eliminated. The method for measuring the concentration of urinary trypsin inhibitors comprises mixing an urine sample, a protease solution containing trypsin, and a buffer solution, adding a substrate solution to the mixture to cause the enzyme reaction, and measuring the activity of the enzyme, wherein the buffer solution is prepared so that it contains at least 0.15 .mu.mol calcium per 1 .mu.g of the trypsin but no more than 100 .mu.

Abstract: A method for fixing a reagent layer directly onto a supporting base plate in the preparation of a dry analysis kit for determining a specific component in a liquid specimen wherein at least one of the reagent layer and the base plate is a thermoplastic resin, and which comprises the steps of:placing the reagent layer in contact with the base plate and externally applying ultrasonic vibration and pressure to the two layers to generate frictional heat thereby to melt the surface of the thermoplastic resin;applying pressure to make the molten surface of thermoplastic resin bite the non-thermoplastic material or to integrate the surfaces of thermoplastic layer and base plate; andremoving the ultrasonic vibration and pressure.

Abstract: Light of a specific wavelength is introduced into respective optical fiber members forming a light projecting optical fiber member group (14a) through a condensing lens (26). A second end of an optical fiber light guide path (10) whose first end is brought into close contact with a target (20) is branched into three optical fiber member groups including a light projecting optical fiber member group (14a) and first and second photoreceiving optical fiber member groups (16a, 18a), and each of the optical fiber member groups (14a, 16a, 18a) is formed by bundling optical fiber members forming respective unit bundles respectively.