Abstract: An automatic loading apparatus (12) for specimens (1) for high-pressure cryosubstitution is disclosed. The automatic loading apparatus (12) is connected to a high-pressure freezing device (40). Specimens (1) are inserted in a holder (2). By means of a slider (4), the specimens (1) and holders (2) are conveyed to the automatic loading apparatus (12). The guidance element (26) allows exact positioning of the holder (2) in the clamping element (14). The automatic loading apparatus makes possible rapid transfer of the holder (2) having the specimen (1) from, for example, an optical microscope to the high-pressure freezing device (40).

Abstract: An apparatus (1) for tissue preparation for the tissue embedding, having a transport plate (15) defining a circumferential edge (15c). At the circumferential edge (15c) several recesses (16) are formed, each of which comprises a plurality of several circular cut outs (16a) being arranged so that it has an opening into the direction of the circumferential edge (15c). The transport plate (15) is surrounded by a housing (5) which defines an inner upper side (8a) on which a heating and cooling device (3) is provided.

Abstract: An apparatus and a method for immunological labeling of thin tissue sections are disclosed. The treatment fluid is applied in the form of liquid droplets onto at least one slide, the arrangement of liquid droplets corresponding to the arrangement of the thin tissue sections on the carrier plate. Each slide is retained in a transport container, the transport container being embodied in the form of a trough which comprises a peripheral delimiting wall that is closed off by a base. The base of one transport containers constitutes the cover of a transport container arranged beneath it. The transport containers are stacked in a first and a second station.

Abstract: An apparatus and a method for cryosubstitution and embedding of biological specimens is disclosed. The apparatus encompasses a container (6) for receiving at least one specimen carrier (2) having a specimen (4). The specimen carriers (2) are placed into troughs (12) of the container (6). Configured in each trough is a step (20) by which the specimen carrier (2), together with the specimen (4), is held in the container (6).

Abstract: A heating and cooling device (3) for an apparatus (1) for tissue preparation for the tissue embedding is disclosed. The apparatus (1) has a free space (13) for the accommodation of a transport plate (15). The apparatus (1) is surrounded by a housing (5) which defines an upper side (8) and a heating and cooling device (3) that is provided on the upper side (8) of the housing (5), and the heating and cooling device (3) has a reception (23) with at least one chamber (32) for the accommodation of at least one processing container (17). The heating and cooling device (3) is pivotable with respect to the upper side (8) of the housing (5).

Abstract: A device 1 and a method for trimming samples 38 are disclosed. The device 1 encompasses a knife holder 9 and a sample holder 7, the knife holder 9 carrying at least one trimming knife 36. A first motor 60 and a second motor 61 are provided, which move the knife holder 9 in an X-Y plane. Also provided is a control unit 15 that controls the motion of the knife holder 9. The first and the second motor 60, 61 are each equipped with a measurement means 62, 63 that measures the position of the knife holder 9 in the X direction (X) and Y direction (Y).

Abstract: A device for sectioning samples, which encompasses a sectioning apparatus for generating thin sample pieces and a table for placement of the sectioning apparatus thereon. Displaceable arm supports, which are adjustable to a user's ergonomics and to a configuration of the device, are provided on the table opposite the knife holder and sample holder of the sectioning apparatus.

Abstract: A coolant hose for the transport of coolant from a reservoir vessel to a cooling chamber is disclosed. The coolant hose is constructed from a transport hose and an insulating casing. The first and the second end of the coolant hose are respectively provided with a rotatable and thermally insulated connecting element that coacts with a corresponding connecting element on the cooling chamber and on the reservoir vessel.

Abstract: A cooling chamber 10 for a microtome 1 is disclosed. A knife 2 having a cutting edge 16 is arranged in the cooling chamber 10 opposite a sample holder 4 having a sample 4a retained therein. The microtome 1 further possesses a stereomicroscope 12 having an optical system 14, the optical system 14 defining an optical axis 11. The region of the sample 4a and of the cutting edge 16 of the knife 2 is observable with the stereomicroscope 12. There is mounted in the cooling chamber 10 an illumination system 20 that emits light 20a and is directed onto a surface 2a of the knife 2 in such a way that the light 20a reflects from the surface 2a parallel to the optical axis 11.

Abstract: An apparatus for controlling a microtome (2) and a cooling chamber (4) provided therefor is disclosed. Control is accomplished by way of a single control unit (12, 18) that integrates both operating elements (30) for the microtome (2) and operating elements (32) for the cooling chamber (4).

Abstract: In cutting devices (10), in particular microtomes or ultramicrotomes, it is necessary to preset the specimen (14) onto the knife (16). To simplify this presetting operation, which is intended to ensure the quickest and most accurate possible positioning of the knife (16) relative to the specimen (14), a stereomicroscope (12) is provided that can be adjusted in many ways using operating elements (15, 17, 19, 27). To improve the unit's ergonomics and additionally eliminate collisions during pivoting, the unit base (22) of the cutting device (10) is modified. Since the modification of the unit base must result in a lowering, this modification can be achieved by lowering the knife holder (26), the mounting surface for the linear guide being milled directly onto the unit base (22).

Abstract: In cutting devices (10), in particular microtomes or ultramicrotomes (24), it is necessary to preset the specimen onto the knife (14). To improve the accuracy and reproducibility of this presetting operation, which is intended to ensure the quickest and most accurate possible positioning of the knife (14) relative to the specimen, a pivotable observation device (10), in particular a stereomicroscope, is provided. The observation device (10) is coupled to a pivoting device (28) that comprises a positioning device (29). The positioning device (29) is embodied in such a way that a positioning of the observation device (10) at a defined angle is possible.

Abstract: An apparatus (1) for handling coverslips (20) for specimen slides (9) comprises a transport arm (8), a base block (3) mounted on the transport arm and having a having a notional center axis (3a), and at least two suction devices (4 and 5) carried by the base block (3), each suction device (4 and 5) having an end (4a and 5a) that comes into contact with a coverslip (2) and points away from the notional center axis (3a) of said base block (3). The base block (3) both removes a single coverslip (2) from a magazine (24), and deposits the removed coverslip (2) onto a specimen slide (9).

Abstract: In microtomes or ultramicrotomes, it is necessary to preset the specimen (14) onto the knife (16). In order to facilitate and automate this presetting operation, which is intended to guarantee the fastest and most accurate possible positioning of the knife (16) relative to the specimen (14), the spacing (13) between the trimmed surface (15) of the specimen (14) and the specimen holder (22) is ascertained and is transferred to the microtome or ultramicrotome (10). The spacing (13) is preferably ascertained in the trimming device (12).

Abstract: A transport container (40) for slides (4) for immunological labeling of thin tissue sections (2a) is disclosed. The transport container (40) is embodied in the form of a trough which comprises a peripheral delimiting wall (49) that is closed off by a base (50). There is embodied in the interior of the trough on the delimiting wall (49) at least one peripheral step (57) on which the slide rests and is spaced away from the base (50) of the transport container (40).

Abstract: An apparatus and a method for immunological labeling of thin tissue sections are disclosed. The treatment fluid is applied in the form of liquid droplets onto at least one slide, the arrangement of liquid droplets corresponding to the arrangement of the thin tissue sections on the carrier plate. Each slide is retained in a transport container, the transport container being embodied in the form of a trough which comprises a peripheral delimiting wall that is closed off by a base. The base of one transport containers constitutes the cover of a transport container arranged beneath it. The transport containers are stacked in a first and a second station.

Abstract: A specimen holder (10) is proposed in order to create a capability for preparing, in a cutting device, in particular a microtome or ultramicrotome, a specimen that is to examined in an AFM. The specimen holder (10) is embodied in several parts. It comprises an insert (12) in which the specimen is secured. Also provided is a receiving ring (14) in which the insert (12) can be received. The insert together with the receiving ring (14) is mounted, in particular thread-joined, on a base element (16). As a result of the mounting of the receiving ring (14) on the base element (16), the insert together with the specimen is fixed in its position.

Abstract: The invention concerns a unit (2) for automatic delivery of specimen slides to a coverslipper (3). The unit (2) comprises a housing (27) in which a front panel (25) is provided. Also provided is a passage (20) in the housing (27) that is defined substantially by a first and a second cutout (21 and 23) in the first and the second side wall (22 and 24). A transfer position (8) for racks (4) is provided in the region of the cutout (21) in the first side wall (22), and a transport apparatus (9) extends out substantially from the transfer position (8) to beyond the cutout (23) in the second side wall (24).

Abstract: An apparatus for handling biopsy specimens comprises a transfer part, a slide displaceable in a groove of said transfer part, preparation plates for the reception of biopsy specimens, and a shifter part that serves to introduce the biopsy specimens into the preparation plates. The empty preparation plates are positioned at a predetermined point in the groove of the transfer part, and the shifter part is placed onto the transfer part above the preparation plates. The biopsy specimens are then introduced into the specimen well of a preparation plate by manually actuated instruments or by means of a stream of fluid. The filled preparation plate is displaced back in the groove of the transfer part using the slide, and is transferred into a specimen holder prepared for it.

Abstract: A specimen holder for freezing water-containing preparations under high pressure is disclosed. The specimen holder possesses a housing sheath in which a cutout is provided. A specimen retention element is arranged in the cutout of the specimen holder, and can be sprayed with a coolant, from both sides, through the cutout. The specimen retention element is made up of at least a first part and a second part, a recess for holding the specimen is shaped in the second part. The second part is pressed with a screw against the first part in such a way that the recess is sealed in pressure-tight fashion. Also disclosed are a method and system for freezing water-bearing specimens under high pressure.