Patents Assigned to National University Corporation Kobe University
  • Patent number: 11041826
    Abstract: Provided is a measurement device including an application unit, a detection unit, and a calculation unit. The application unit applies a first magnetic field, which is generated by applying a pulse current to a coil or applying currents with a plurality of frequencies to the coil in order, to an object. The detection unit detects a second magnetic field which is generated by applying the first magnetic field to the object. The calculation unit calculates a distribution of a magnetic field source m in the second magnetic field. The calculation unit may further generate an imaging signal for displaying the calculated distribution of the magnetic field source m, as an image. The display unit displays the image indicating the distribution of the magnetic field source m by using the imaging signal.
    Type: Grant
    Filed: November 15, 2016
    Date of Patent: June 22, 2021
    Assignees: National University Corporation Kobe University, Integral Geometry Science Inc.
    Inventors: Kenjiro Kimura, Noriaki Kimura
  • Patent number: 11040087
    Abstract: Provided is a therapeutic agent that effectively acts on a disease associated with abnormal glycosylation of dystroglycan. Also provided is a testing method for diseases associated with abnormal glycosylation of dystroglycan. Specifically, provided is a therapeutic agent for diseases associated with abnormal glycosylation of dystroglycan, containing CDP-ribitol as an active ingredient. Ribitol-phosphate is important in the glycan structure of dystroglycan. In order for ribitol-phosphate to be incorporated into a dystroglycan glycan, a material therefor (sugar donor) is required. In the present invention, it has been found for the first time that CDP-ribitol serves as the sugar donor. It has been confirmed that the glycan of ISPD-deficient cells can be restored by administering CDP-ribitol. Thus, the present invention, which allows CDP-ribitol to be utilized for supplementation therapy, has been completed.
    Type: Grant
    Filed: August 18, 2017
    Date of Patent: June 22, 2021
    Assignees: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, TOKYO METROPOLITAN GERIATRIC HOSPITAL AND INSTITUTE OF GERONTOLOGY, OSAKA PREFECTURAL HOSPITAL ORGANIZATION
    Inventors: Tatsushi Toda, Kazuhiro Kobayashi, Motoi Kanagawa, Tamao Endo, Hiroshi Manya, Yoshinao Wada, Michiko Tajiri
  • Patent number: 11041169
    Abstract: The present invention provides a method for modifying a targeted site of a double-stranded DNA in a cell, the method including a step of bringing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a selected target nucleotide sequence in a double-stranded DNA and a nucleic acid base converting enzyme or DNA glycosylase are linked, and a donor DNA containing an insertion sequence into contact with said double-stranded DNA, to substitute the targeted site with the insertion sequence, or insert the insertion sequence into said targeted site, without cleaving at least one strand of said double-stranded DNA in the targeted site.
    Type: Grant
    Filed: March 26, 2019
    Date of Patent: June 22, 2021
    Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventor: Keiji Nishida
  • Publication number: 20210180073
    Abstract: One objective of the present invention is to provide a novel simple and efficient transformation method for gram-positive bacteria, the transformation method being capable of introducing a large-sized DNA into a host DNA of the gram-positive bacteria without damage. In addition, another objective of the present invention is to provide a method in which desired DNA segments are accumulated in a chromosome of a recipient (recipient bacteria) to enable an artificially designed huge DNA to be constructed, and in which a transformed cell does not cause any problems in terms of controlling the natural environment. The present invention relates to a transformation method for gram-positive bacteria by conjugative transfer, characterized to use a helper plasmid having an origin of DNA transfer (oriT) region is inactivated. Preferably, the helper plasmid is a plasmid in which an oriT region is inactivated from pLS20cat.
    Type: Application
    Filed: December 7, 2018
    Publication date: June 17, 2021
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventor: Kenichi YOSHIDA
  • Publication number: 20210171935
    Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Application
    Filed: February 12, 2021
    Publication date: June 10, 2021
    Applicant: National University Corporation Kobe University
    Inventors: Keiji NISHIDA, Akihiko KONDO
  • Publication number: 20210155707
    Abstract: An anti-SIRP? antibody that can be used as a tumor agent and an anti-tumor agent comprising the antibody as an active ingredient. An antibody that binds specifically to human SIRP? to inhibit binding of human SIRP? to CD47.
    Type: Application
    Filed: July 9, 2019
    Publication date: May 27, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, DAIICHI SANKYO COMPANY, LIMITED
    Inventors: Takashi MATOZAKI, Mayumi SUE, Kensuke NAKAMURA, Chigusa YOSHIMURA
  • Patent number: 11001856
    Abstract: The present invention provides a method for modifying a targeted site of a double-stranded DNA in a cell, the method including a step of bringing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a selected target nucleotide sequence in a double-stranded DNA and a nucleic acid base converting enzyme or DNA glycosylase are linked, and a donor DNA containing an insertion sequence into contact with said double-stranded DNA, to substitute the targeted site with the insertion sequence, or insert the insertion sequence into said targeted site, without cleaving at least one strand of said double-stranded DNA in the targeted site.
    Type: Grant
    Filed: March 26, 2019
    Date of Patent: May 11, 2021
    Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventor: Keiji Nishida
  • Patent number: 10994056
    Abstract: A device for fixing biological soft tissue is endowed with strength and deformation performance for being used as a device for coupling biological soft tissue that has been cut or separated due to an incision or the like during a surgical procedure, and is completely degraded in vivo and discharged after adhesion of the soft tissue or after healing of the incision tissue. The device is composed of a ternary Mg alloy material of Mg—Ca—Zn. In the Mg alloy material, the Ca and Zn are contained within the solid-solubility limit with respect to the Mg. The remainder is composed of Mg and unavoidable impurities. The Zn content is 0.5 at % or less. The Ca and Zn content has a relationship of Ca:Zn=1:x (where x is 1 to 3) by atom ratio. The crystal grain structure is equiaxed, the crystal grain size according to linear intercept being 30 to 250 ?m.
    Type: Grant
    Filed: September 9, 2015
    Date of Patent: May 4, 2021
    Assignee: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Toshiji Mukai, Naoko Ikeo, Eisei Gu, Takumi Fukumoto, Hikaru Yabuuchi
  • Publication number: 20210115287
    Abstract: The object of the present invention is to provide a coating agent for forming a coating film having barrier properties and stretchability and to provide a resin container comprising a coating film formed using the coating agent on a surface thereof, wherein the coating film has high barrier properties and is less prone to film breakage during stretching. LDH, which is in the form of nanometer-scale particles, is added along with PVA.
    Type: Application
    Filed: June 5, 2019
    Publication date: April 22, 2021
    Applicants: Suntory Holdings Limited, NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Masanori Nishiyama, Ichiro Tomari, Ryo Yamane, Hideyuki Suzuki, Koji Kuraoka
  • Publication number: 20210086140
    Abstract: Providing a membrane-forming solution suitable for producing a separation membrane such as a hollow fiber membrane and a flat membrane. A membrane-forming solution including triacetylcellulose having an acetyl group substitution degree of 2.7 or higher, a good solvent for thermally induced phase separation and a poor solvent for thermally induced phase separation, wherein the good solvent is capable of heat-dissolving the triacetylcellulose (at a solid content concentration of 25 mass %), and the poor solvent is incapable of dissolving the triacetylcellulose up to the heat dissolution temperature of the good solvent, wherein both the good solvent and the poor solvent are included so as to enable phase separation of the heat-dissolved triacetylcellulose solution while the heat-dissolved triacetylcellulose solution is cooled to room temperature (from 20 to 30° C.
    Type: Application
    Filed: July 24, 2018
    Publication date: March 25, 2021
    Applicants: DAICEL CORPORATION, NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Hideto MATSUYAMA, Sungil JEON, Shota TAKAO, Toyozo HAMADA
  • Patent number: 10928363
    Abstract: At least one stable isotope reagent is added to each biological sample and standard sample to prepare biological samples for analysis and standard sample for analysis. The quality of the biological samples is evaluated using data of one set of biological samples for analysis composed of a plurality of biological samples for analysis. Besides, the quality of a pretreatment and/or analysis of each set of samples for analysis is evaluated using data obtained by analyzing the standard sample for analysis before and after an analysis of one set of samples for analysis. An abnormality in a chromatograph or mass analyzer used for the analysis of one set of samples is evaluated by the data obtained by analyzing a sample for device evaluation before and after the analysis of one set of samples for analysis. Thus, the quality of data obtained by chromatographic mass spectrometry on biological samples is comprehensively evaluated.
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: February 23, 2021
    Assignees: SHIMADZU CORPORATION, NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Noriyuki Ojima, Shuichi Kawana, Yumi Unno, Takero Sakai, Kenichi Obayashi, Yukihiko Kudo, Katsuyuki Taneda, Masaru Yoshida, Shin Nishiumi, Takashi Kobayashi, Takeshi Azuma
  • Publication number: 20210046214
    Abstract: A therapeutic agent for intervertebral disc degeneration that contains LASCol obtained by enzymatically cleaving a terminus of collagen.
    Type: Application
    Filed: January 31, 2019
    Publication date: February 18, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, KINKI UNIVERSITY, FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE
    Inventors: Takashi YURUBE, Yoshiki TAKEOKA, Koichi MORIMOTO, Saori KUNII, Kaoru OMAE
  • Patent number: 10920215
    Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Grant
    Filed: November 2, 2015
    Date of Patent: February 16, 2021
    Assignee: National University Corporation Kobe University
    Inventors: Keiji Nishida, Akihiko Kondo
  • Publication number: 20210024906
    Abstract: The present invention provides a complex containing a nucleic acid sequence-recognizing module and a proteolysis tag, wherein the module is linked to the proteolysis tag, the module specifically binds to a target nucleotide sequence in a double stranded DNA, and the tag consists of (i) a peptide containing 3 hydrophobic amino acid residues at the C-terminal, or (ii) a peptide containing 3 amino acid residues at the C-terminal m wherein at least a part of the amino acid residues is substituted by serine.
    Type: Application
    Filed: November 21, 2018
    Publication date: January 28, 2021
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventor: Keiji NISHIDA
  • Publication number: 20210017528
    Abstract: One or more embodiments of the present invention are to provide a new means of improving the productivity of a target protein. The present inventors have identified a novel protein consisting of the amino acid sequence set forth in SEQ ID NO: 2 through an exhaustive analysis of the nucleotide sequence of chromosomal DNA of a yeast belonging to the genus Komagataella. Activation of a gene encoding the novel protein provides a cell having an improved productivity of a target protein.
    Type: Application
    Filed: September 25, 2020
    Publication date: January 21, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, KANEKA CORPORATION
    Inventors: Yoichiro Ito, Jun Ishii, Yasuyuki Nakamura, Akihiko Kondo, Teruyuki Nishi
  • Publication number: 20200377910
    Abstract: The present invention provides a method of modifying a targeted site of a double-stranded DNA, comprising a step of introducing a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double-stranded DNA and PmCDA1 are bonded, into a cell containing the double-stranded DNA, and culturing the cell at a low temperature at least temporarily to convert the targeted site, i.e., the target nucleotide sequence and nucleotides in the vicinity thereof, to other nucleotides, or delete the targeted site, or insert nucleotide into the site.
    Type: Application
    Filed: April 21, 2017
    Publication date: December 3, 2020
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji NISHIDA, Akihiko KONDO, Takayuki ARAZOE, Zenpei SHIMATANI
  • Publication number: 20200317876
    Abstract: A method is for treating a surface of a resin material layer. The method includes a first step of introducing as a substituent at least one selected from the group consisting of an acid halide and an alkyl halide into an aromatic polyether-based resin included in the resin material layer by Friedel-Crafts reaction.
    Type: Application
    Filed: March 31, 2017
    Publication date: October 8, 2020
    Applicants: MITSUBISHI HEAVY INDUSTRIES, LTD., National University Corporation KOBE University
    Inventors: Koichi HASEGAWA, Toshio ABE, Kiyoka TAKAGI, Takashi NISHINO, Takuyua MATSUMOTO, Akira MIYAGAKI
  • Patent number: 10792102
    Abstract: A laser tip, a laser treatment tool, a laser treatment device and a laser treatment system are provided for performing laser treatment surgery and minimizing any bleeding. A laser tip is attachable to a laser radiation opening located at the tip end of a laser transmission tube. The laser tip includes an attaching portion detachable from the laser radiation opening and a contact portion contactable with biological tissue. The contact portion is at the front of the attaching portion in the direction in which the laser light exits the laser radiation opening. An open space is located between the contact portion and the attaching portion, and a coupler couples a part of the contact portion and a part of the attaching portion. The contact portion has a reflective surface at a rear end thereof which reflects the laser light toward the coupler.
    Type: Grant
    Filed: March 18, 2018
    Date of Patent: October 6, 2020
    Assignees: J. Morita Mfg. Corp., National University Corporation Kobe University
    Inventors: Yoshihide Okagami, Akihito Hongo, Yoshiteru Tamura, Katsumi Hiyoshi, Haruhiko Murakami, Takeshi Azuma, Yoshinori Morita
  • Patent number: 10788812
    Abstract: In the present invention: a plurality of meshes are defined on an object surface; the luminance of the object surface when the object surface is viewed from a viewpoint position is calculated for each of the plurality of meshes on the basis of data concerning a processed shape, a surface roughness curve, the viewpoint position, the direction, angle distribution, and intensity of incident light onto the object surface, and a reflectance and scattering characteristics for each wavelength on the object surface; the shape of the object surface is displayed on the basis of the luminances; and data concerning at least the object shape or the surface roughness curve is corrected so as to obtain a desired appearance of the object surface.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: September 29, 2020
    Assignees: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, MAKINO MILLING MACHINE CO., LTD.
    Inventors: Ryuta Sato, Takumi Nakanishi, Mitsunari Oda, Nobu Nakayama
  • Patent number: 10776484
    Abstract: Provided is an on-chip monitor circuit mounted on a semiconductor chip that is equipped with a security function module for performing a security function process on an input signal and outputting a security function signal, the on-chip monitor circuit comprising a monitor circuit for monitoring signal waveforms of the semiconductor chip, wherein the circuit is provided with a first storage means for storing data that designates a window period in which to perform a test of the semiconductor chip, and a control means for performing control to operate the circuit during the window period, when a prescribed test signal is inputted to the security function module. By using the on-chip monitor circuit in a semiconductor chip of which security is required, security attacks, e.g., a Trojan horse or the like, intended to embed a malicious circuit in the production stage of security function module-equipped semiconductors chips, can be prevented.
    Type: Grant
    Filed: January 12, 2016
    Date of Patent: September 15, 2020
    Assignees: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, TELECOM PARISTECH
    Inventors: Makoto Nagata, Jean-Luc Danger, Daisuke Fujimoto, Shivam Bhasin