Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.

Abstract: A solid-liquid boundary detection device 20 is a device for detecting a boundary 141 between a solid and a liquid in a test tube 14, and includes a surface illumination 21, an imaging unit 22, and a boundary detection processor 31. The surface illumination 21 illuminates the test tube 14 from outside. The imaging unit 22 is arranged on an opposite side of the test tube 14 from the surface illumination 21, and captures an image including the boundary 141. The boundary detection processor 31 detects the boundary 141 from the image captured by the imaging unit 22.

Abstract: Provided is a ??T cell for securing the purity and number of cells sufficient for treatment. Also provided is a method of generating the ??T cell. More specifically, provided are homogeneous ??T cells excellent in that the ??T cells are not affected by exhaustion of the cells. The foregoing is achieved by ??T cells obtained by subjecting induced pluripotent stem cells (iPS cells) to differentiation induction treatment. Specifically, the foregoing is achieved by ??T cells generated by subjecting iPS cells having a rearranged ??TCR gene (??TCR-type iPS cells) to differentiation induction treatment. According to the method of generating the ??T cell of the present invention, there can be provided ??T cells and a cell population of ??T cells that have an excellent function of having antigen-specific cytotoxic activity in a MHC-unrestricted manner, and that are more homogeneous and have a higher effect than ??T cells separated from peripheral blood.

Abstract: The invention provides a site-specific modification method of a DNA molecule that enables gene function manipulation. In particular, the invention provides a method for manipulating gene function by a site-specific action on a target DNA molecule, said method comprising a step for preparing a complex containing an Argonaute protein, a guide RNA and an additional sequence and a step for contacting the target DNA molecule with the complex, wherein the guide RNA is designed to guide the complex to a region containing the desired site in the target DNA molecule.

Abstract: Provided is an orally administrable vaccine against a coronavirus infectious disease. A transformed Bifidobacterium designed to display a part or a whole of a constituent protein of a coronavirus on a surface of the Bifidobacterium enables the provision of the orally administrable vaccine against a coronavirus infectious disease. The transformed Bifidobacterium designed to display a part or a whole of a constituent protein of a coronavirus on a surface of the Bifidobacterium can induce humoral immunity and cellular immunity through oral administration to suppress an increase in severity of pneumonia or the like even after viral infection.

Abstract: A holographic three-dimensional multi-spot light stimulation device is provided with: a three-dimensional imaging holographic optical system A which employs fluorescent exciting light to acquire three-dimensional fluorescence distribution information resulting from fluorescent signal light from a plurality of stimulation target objects; and a three-dimensional light stimulation holographic optical system B which employs a light stimulation hologram generated on the basis of the acquired three-dimensional fluorescence distribution information to form a plurality of light spots in space, to impart stimulation simultaneously to the plurality of stimulation target objects.

Abstract: Expression of matrix metalloproteinase 1 is suppressed. A matrix metalloproteinase 1 expression suppression agent of this disclosure contains, as an active ingredient, at least one selected from the group consisting of Saxifraga sarmentosa, an extract of Saxifraga sarmentosa, rosemary, an extract of rosemary, licorice, an extract of licorice, Melissa officinalis, an extract of Melissa officinalis, wild thyme, an extract of wild thyme, hop, and an extract of hop.

Abstract: A federated learning system in which a plurality of local servers repeatedly learn cooperatively through communications between the plurality of local servers and a central server via a network. The local server includes a decryption unit, a mean gradient calculation unit, a model updating unit, a validation error calculation unit, an encryption unit, and a local transmission unit that transmits at least one of a current local mean gradient and a current local validation error. The central server includes a central reception unit, a model selection unit, a weight determination unit, and a central transmission unit. The central reception unit receives encrypted current local models and at least one of current local training data counts, the current local mean gradients, and the current local validation errors from the plurality of respective local servers.

Abstract: The present invention relates to an improved technology for a device that identifies and diagnoses an environmental stress state of plants. An environmental stress diagnosis device comprises a measurement light source 12, an induction light source 14, and a transmitted light detector 18. The measurement light source 12 radiates a first measurement light ML1 and a second measurement light ML2 to a plant sample S, the induction light source 14 radiates a first photosynthesis inducing light FR and a second photosynthesis inducing light AL to the plant sample S, and the transmitted light detector 18 detects a first transmitted light TL1 and a second transmitted light TL2. The control unit 20 has an analysis circuit 20a and a control circuit 20b.

Abstract: The present invention relates to an improved technology for a device that identifies and diagnoses an environmental stress state of plants. A control circuit 20b controls a measurement light source 12 such that a second measurement light ML2 has higher power than a first measurement light ML1 and the first measurement light ML1 and the second measurement light ML2 become opposite-phase rectangular waves, and further controls the measurement light source 12 such that the first measurement light ML1 and the second measurement light ML2 are output in synchronization to form the first measurement light ML1 and the second measurement light ML2 into a quasi-single composite rectangular wave measurement light ML3 of 5 kHz to 30 kHz. A transmitted light detector 18 detects the composite rectangular wave measurement light ML3 transmitted through a plant sample S as a composite rectangular wave transmitted light TL.

Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.

Abstract: The objective of the present invention is to provide a method for producing a carbonyl halide efficiently to the used halogenated hydrocarbon. The method for producing a carbonyl halide according to the present invention is characterized in comprising the steps of preparing a mixed gas comprising oxygen and a C2-4 halogenated hydrocarbon having one or more halogeno groups selected from the group consisting of chloro, bromo and iodo, and flowing the mixed gas and irradiating a high energy light to the flowed mixed gas.

Abstract: A virus inactivation method is provided. The method includes irradiating a solution containing an artificially expressed antibody or antibody fragment with ultraviolet rays in the presence of a radical scavenger. The irradiating only aggregates 5% or less of the antibody or antibody fragment. A wavelength of the ultraviolet rays is 200 nm or longer and 315 nm or shorter. An irradiation amount of the ultraviolet rays is 300 mJ/cm2 or more. An irradiation time of the ultraviolet rays is 3 minutes or less. A concentration of the radical scavenger in the solution is 0.03 mM or more.

Abstract: The objective of the present invention is to provide a method for producing a carbonyl halide efficiently to the used halogenated methane. The method for producing a carbonyl halide according to the present invention is characterized in comprising the steps of preparing a mixed gas comprising oxygen and a halogenated methane having one or more halogeno groups selected from the group consisting of chloro, bromo and iodo, and flowing the mixed gas and irradiating a high energy light to the flowed mixed gas.

Abstract: Provided is a medical immobilization device that contributes to reducing the burden on a patient, while being capable of firmly immobilizing the patient. A medical immobilization device 10 is a device for immobilizing a specific site 1 of the body of a patient with the patient lying on their back. The medical immobilization device 10 comprises a block 3 made of a material that allows radiation to pass through, and the block 3 comprises holder portions 2a to 2c in which at least a region from the lower surface to both sides of the specific site 1 fit. All or part of the upper part of the block 3 is open.

Abstract: A sampling apparatus 1 samples a culture medium in a bioreactor 11. A pump 21 circulates the culture medium in the bioreactor 11 via a flow path 41 by leading out the culture medium from the bioreactor 11 into the flow path 41 and introducing the culture medium from the flow path 41 into the bioreactor 11. A valve 22 is provided in the middle of the flow path 41, and switches a flow path such that the culture medium circulated in the flow path 41 flows out to a branch flow path 42 to be sampled.

Abstract: The invention provides a miniaturized cytidine deaminase-containing complex for modifying DNA formed by combining a nucleic acid sequence recognition module and cytidine deaminase, wherein the nucleic acid sequence recognition module specifically binds to a target nucleotide sequence of double-stranded DNA, the cytidine deaminase is composed of an amino acid sequence composed of a region of amino acid residues at positions 30-150 of SEQ ID NO: 1, an ortholog thereof, an amino acid sequence having mutations of one or several amino acids therein, or an amino acid sequence having at least 90% similarity thereto, and the targeted site of the double-stranded DNA is modified.

Abstract: The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.

Abstract: An analysis method includes: performing liquid chromatography using a mobile phase including an adsorption prevention agent for preventing adsorption of a sample including a compound having a phosphate group to metal; and performing mass spectrometry on an eluate of the liquid chromatography. The adsorption prevention agent includes an oxalic acid or a salt of the oxalic acid.

Abstract: It is an object to provide a combination therapy effective in cancer immunotherapy. The object is achieved by providing an anti-tumor agent, including a transformed Bifidobacterium containing DNA encoding a WT1 protein and DNA encoding a GNB/LNB substrate-binding membrane protein derived from a Bifidobacterium, the transformed Bifidobacterium being designed to display the WT1 protein as an antigen on a surface of the transformed Bifidobacterium, the anti-tumor agent being for use in combination with an immunosuppression inhibitor. The transformed Bifidobacterium can be used as an oral tumor vaccine.