Abstract: The present invention provides a method of modifying a targeted site of a double-stranded DNA, comprising a step of introducing a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double-stranded DNA and PmCDA1 are bonded, into a cell containing the double-stranded DNA, and culturing the cell at a low temperature at least temporarily to convert the targeted site, i.e., the target nucleotide sequence and nucleotides in the vicinity thereof, to other nucleotides, or delete the targeted site, or insert nucleotide into the site.

Abstract: A method is for treating a surface of a resin material layer. The method includes a first step of introducing as a substituent at least one selected from the group consisting of an acid halide and an alkyl halide into an aromatic polyether-based resin included in the resin material layer by Friedel-Crafts reaction.

Abstract: A laser tip, a laser treatment tool, a laser treatment device and a laser treatment system are provided for performing laser treatment surgery and minimizing any bleeding. A laser tip is attachable to a laser radiation opening located at the tip end of a laser transmission tube. The laser tip includes an attaching portion detachable from the laser radiation opening and a contact portion contactable with biological tissue. The contact portion is at the front of the attaching portion in the direction in which the laser light exits the laser radiation opening. An open space is located between the contact portion and the attaching portion, and a coupler couples a part of the contact portion and a part of the attaching portion. The contact portion has a reflective surface at a rear end thereof which reflects the laser light toward the coupler.

Abstract: In the present invention: a plurality of meshes are defined on an object surface; the luminance of the object surface when the object surface is viewed from a viewpoint position is calculated for each of the plurality of meshes on the basis of data concerning a processed shape, a surface roughness curve, the viewpoint position, the direction, angle distribution, and intensity of incident light onto the object surface, and a reflectance and scattering characteristics for each wavelength on the object surface; the shape of the object surface is displayed on the basis of the luminances; and data concerning at least the object shape or the surface roughness curve is corrected so as to obtain a desired appearance of the object surface.

Abstract: Provided is an on-chip monitor circuit mounted on a semiconductor chip that is equipped with a security function module for performing a security function process on an input signal and outputting a security function signal, the on-chip monitor circuit comprising a monitor circuit for monitoring signal waveforms of the semiconductor chip, wherein the circuit is provided with a first storage means for storing data that designates a window period in which to perform a test of the semiconductor chip, and a control means for performing control to operate the circuit during the window period, when a prescribed test signal is inputted to the security function module. By using the on-chip monitor circuit in a semiconductor chip of which security is required, security attacks, e.g., a Trojan horse or the like, intended to embed a malicious circuit in the production stage of security function module-equipped semiconductors chips, can be prevented.

Abstract: A shape evaluation method includes a shape error calculation step that calculates a shape error, which is an error between a designed shape and a shape to be evaluated, and a visible error detection step that detects visible shape errors on the basis of the shape error and predetermined visual characteristic data.

Abstract: The invention provides a method of modifying a targeted site of gram-positive bacterium of a double stranded DNA. The method includes contacting the double-stranded DNA with a complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a given double stranded DNA and a nucleic acid base converting enzyme to convert, delete, or insert one or more nucleotides in the targeted site without cleaving at least one strand of the double stranded DNA in the targeted site, by introducing the nucleic acid encoding the complex into the gram-positive bacterium. The invention also provide a nucleic acid-modifying enzyme complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double stranded DNA of a gram-positive bacterium and a nucleic acid base converting enzyme bonded to each other, which complex is used for the method.

Abstract: The present invention provides a method for modifying a targeted site of a double-stranded DNA in a cell, the method including a step of bringing a complex in which a nucleic acid sequence-recognizing module that specifically binds to a selected target nucleotide sequence in a double-stranded DNA and a nucleic acid base converting enzyme or DNA glycosylase are linked, and a donor DNA containing an insertion sequence into contact with said double-stranded DNA, to substitute the targeted site with the insertion sequence, or insert the insertion sequence into said targeted site, without cleaving at least one strand of said double-stranded DNA in the targeted site.

Abstract: A method of cultivating algal cells of an algae belonging to a class selected from Chlorophyceae, Euglenophyceae, Bacillariophyceae and Haptophyceae includes: irradiating the algal cells with an artificial light having a ratio of (i) photon flux density in a wavelength range of 520-630 nm to (ii) photosynthetic photon flux density, that is 65% or more; and measuring a condition of the algal cells and/or a condition of an algal cell culture provided by cultivating the algal cells. Irradiation and non-irradiation of the algal cells with the artificial light are switched, or the photon flux density in the wavelength range of 520-630 nm is changed, according to the measured condition of the algal cells and/or the measured condition of the algal cell culture.

Abstract: A laminate exhibiting structural color is characterized by including: a base material layer having a plurality of projections or a plurality of recesses substantially regularly arranged on a surface thereof; a first layer that is a metal layer laminated on the surface of the base material layer so as to have a metallic microstructure enabling surface plasmonic resonance; and a second layer laminated on the first layer and made of a material that more easily absorbs visible light than a metal forming the first layer.

Abstract: A digital holographic microscope in which two digital holographic microscopes for detecting a fluorescence image and a phase image, respectively, are combined to be able to three-dimensionally measure a fluorescence image and a phase image at the same time, and perform measurement at a high SN ratio in all the polarization states including random light polarization. A first holographic optical system that, by using laser light, acquires a phase three-dimensional image due to interference light generated by superimposing object light which passes through a sample stage and reference light which does not pass through the sample stage onto each other. A second holographic optical system that, by using fluorescent excitation light, acquires a fluorescence three-dimensional image due to a fluorescence signal light, wherein phase measurement by the first holographic optical system and fluorescence measurement by the second holographic optical system are performed at the same time.

Abstract: A cancer vaccine that can be orally administered can be provided through the use of a transformed Bifidobacterium capable of expressing and displaying a WT1 protein. The WT1 protein expressed and displayed by the transformed Bifidobacterium is a protein covering most of a WT1 protein unlike a WT1 peptide vaccine restricted to a certain HLA. A cancer vaccine using the transformed Bifidobacterium as an active ingredient is applicable to patients of various HLA types.

Abstract: The purpose of the present invention is to provide a spinal fusion implant capable of preserving turning properties of vertebral bodies, and in addition, capable of preventing false screwing (false insertion) of screws. Connection plates 3 constituting a spinal fusion implant 101 each have, on a ventral-side surface 3b, a convex-shaped portion 8 that is fitted into a concave-shaped portion of an intervertebral joint 53. In addition, a center hole 3d through which a center rod 4 is slidably inserted is formed on a side surface of the connection plate 3, such that the center of the hole is positioned closer to the back side than a virtual line L1 connecting midpoints on arc-shaped contour lines C of the concave-shaped portions of the adjacent intervertebral joints 53 when viewed from the head side of a human body.

Abstract: The present invention provides a method for maintenance and expansion of a colon cancer stem cell or induction of a colon cancer organoid. In addition, the present invention provides a medicament screening system using a colon cancer stem cell maintained and expanded or a colon cancer organoid induced by the method.

Abstract: The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.

Abstract: The objective of the present invention is to provide a method for producing a fluorinated carbonate derivative in a safe and efficient manner. The method for producing a fluorinated carbonate derivative according to the present invention is characterized in comprising irradiating light on a composition containing a C1-4 halogenated hydrocarbon having one or more kinds of halogen atoms selected from the group consisting of a chlorine atom, a bromine atom and an iodine atom, a fluorine-containing compound having a nucleophilic functional group and a base in the presence of oxygen.

Abstract: Disclosed is a method for obtaining information on subject's diabetes, comprising measuring lipoprotein's ability to uptake sterol in a biological sample of a subject, wherein a measured value of ability to uptake sterol per unit volume of the biological sample is an indicator of diabetes onset risk.

Abstract: A forward osmosis membrane characterized in that a thin membrane layer having the performance of a semi-permeable membrane is laminated on a polyketone support layer.

Abstract: The present invention aims at establishing a molecular therapy for Alport syndrome. The present invention provides an oligonucleotide of 15-30 bp comprising a nucleotide sequence complementary to the cDNA of COL4A5 gene, wherein the oligonucleotide is capable of inducing skipping of an exon which has a truncating mutation found in COL4A5 gene in Alport syndrome patients and whose nucleotide number is a multiple of 3, a pharmaceutically acceptable salt thereof, or a solvate thereof. Also provided is a pharmaceutical drug comprising the above oligonucleotide, a pharmaceutically acceptable salt thereof, or a solvate thereof (therapeutic drug for Alport syndrome).

Abstract: The objective of the present invention is to provide a method for producing a carbonate derivative in a safe and efficient manner. The method for producing a carbonate derivative according to the present invention is characterized in comprising irradiating light on a composition containing a C1-4 halogenated hydrocarbon having one or more kinds of halogen atoms selected from the group consisting of a chlorine atom, a bromine atom and an iodine atom, a nucleophilic functional group-containing compound and the specific base in the presence of oxygen.