Abstract: The present invention relates to a method of dewatering post fermentation fluids in a starch to ethanol process. More particularly the invention relates to use of a nuclease enzyme for separation of whole stillage into an insoluble fraction and a supernatant fraction. In a specific embodiment the present invention relates to a method of dewatering whole stillage comprising the steps of: i) subjecting whole stillage to one or more nuclease enzymes; ii) separating the material into an insoluble fraction and a supernatant fraction.
Abstract: The present invention provides means and methods for improving polypeptide expression yield by increasing the number of polypeptides that can be transported into the endoplasmic reticulum (ER) per translocation event.
Type:
Application
Filed:
December 17, 2019
Publication date:
March 10, 2022
Applicant:
Novozymes A/S
Inventors:
Richard Jan Steven Baerends, Carsten Lillelund Olsen, Josè Arnau
Abstract: Disclosed are endonuclease 1 ribonuclease polypeptides and cleaning compositions containing the polypeptides. Also disclosed are methods for using the polypeptides and cleaning compositions. Also disclosed are polynucleotides encoding the polypeptides, and vectors and cells containing the polynucleotides.
Abstract: The present invention relates to a variant of a parent lipase, which parent lipase has the amino acid sequence of SEQ ID NO: 2, wherein the variant has lipase activity, comprises a substitution at a position corresponding to position 51 and comprises a substitution or remains unaltered at a position corresponding to position 96 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Grant
Filed:
December 8, 2015
Date of Patent:
March 8, 2022
Assignee:
Novozymes A/S
Inventors:
Christian Lundager Gylstorff, Christian Jackel, Jesper Vind, Allan Svendsen, Marco Malten, Thomas Agersten Poulsen
Abstract: The present invention relates to prokaryotic host cells and methods for improving expression of a mature protease by co-expression with a propeptide of said protease as two separate polypeptides.
Abstract: The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Application
Filed:
November 19, 2021
Publication date:
March 3, 2022
Applicant:
Novozymes A/S
Inventors:
Lars Henrik Oestergaard, Gernot J. Abel, Dorte Marie Koefoed Klitgaard, Annette Helle Johansen
Abstract: The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
Type:
Application
Filed:
April 30, 2021
Publication date:
March 3, 2022
Applicant:
Novozymes A/S
Inventors:
Frank Winther Rasmussen, Peter Kamp Hansen, Lars Lehmann Hylling Christensen
Abstract: The present invention relates to a mutant of a parent filamentous fungal cell, comprising a coding sequence of a polypeptide of interest under the transcriptional control of a promoter regulated by a transcription factor, wherein the gene encoding the transcription factor is modified in the parent filamentous fungal cell to produce the mutant rendering the mutant deficient in the production of the transcription factor, which increases the productivity of the mutant in the production of the polypeptide of interest, and/or reduces or eliminates the cellulase-negative phenotype in the resulting mutant. The present invention also relates to a method for constructing such a mutant and a method of producing a polypeptide of interest with such a mutant.
Type:
Application
Filed:
December 12, 2019
Publication date:
March 3, 2022
Applicant:
Novozymes A/S
Inventors:
Suchindra Maiyuran, Alfredo Lopez De Leon
Abstract: A nanoparticle comprises water-insoluble polymer matrix and an indicator constituent(s), wherein the indicator constituent(s) is released from the nanoparticle only when the polymer matrix is degraded or broken, and then an indicative effect is triggered or enhanced. A nanoparticle-based method for screening a bioactive substance and a microfluidic-based screening system have also been disclosed.
Type:
Application
Filed:
December 12, 2019
Publication date:
February 24, 2022
Applicants:
Novozymes A/S, Institute of Microbiology, Chinese Academy of Sciences
Inventors:
Wenbing Du, Yuxin Qiao, Dongwei Chen, Shufang Zhao, Ming Li, Ying Zhang, Wanghui Xu, Cheng Peng, Beiyu Hu, Ran Hu
Abstract: Provided herein is a method for producing a protein hydrolysate using a polypeptide having endopeptidase activity and a polypeptide having carboxypeptidase activity and the use of these enzymes for hydrolysing a protein substrate. Also provided are polypeptides having carboxypeptidase activity and polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Grant
Filed:
June 8, 2018
Date of Patent:
February 22, 2022
Assignee:
Novozymes A/S
Inventors:
Hanne Vang Hendriksen, Gitte Budolfsen Lynglev, Henrik Frisner, Ciu Liu, Ye Liu, Eduardo Antonio Della Pia, Hans Peter Heldt-Hansen, Kenneth Jensen, Wei Peng, Ming Li
Abstract: The present invention provides a method of producing a baked product having reduced crumb elasticity, said method comprising adding a raw starch degrading alpha-amylase, a lipase and a phospholipase to dough ingredients comprising flour, water, and yeast and mixing to prepare a dough; leavening the dough; and baking the dough to obtain the baked product.
Abstract: The present invention relates to phytase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Application
Filed:
August 13, 2021
Publication date:
February 17, 2022
Applicant:
Novozymes A/S
Inventors:
Hengxiao Zhai, Jesper Vind, Lars Kobberoe Skov, Qian Zhang, Ester Santigosa, Jose-Otavio B. Sorbara, Aurelia Anne Catherine Charlotte Seon, Carrie Louise Walk
Abstract: The present invention relates to lipase variants and compositions comprising (i) at least one surfactant and (ii) at least one lipase variant of the invention. Furthermore, the present invention relates to methods of using the compositions.
Type:
Application
Filed:
November 3, 2021
Publication date:
February 17, 2022
Applicant:
NOVOZYMES A/S
Inventors:
Kim Borch, Carsten Hørslev Hansen, Jesper Vind, Marco Malten, Svend Gunnar Kaasgaard
Abstract: The present invention relates to pelletized feed compositions comprising granules which comprise an inner salt layer and an outer hydrophobic layer. The invention further relates to the use of salt and hydrophobic coated granules for steam treated pelletized feed compositions.
Abstract: The present invention relates to alpha-amylase variants having an improved stability as compared to the parent alpha-amylase. The invention further relates to use of the variants, compositions comprising the variants, and methods of producing the variants.
Abstract: The present invention relates to isolated polypeptides having alpha-amylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.
Abstract: The present invention relates to compositions such as cleaning compositions comprising a mix of enzymes. The invention further relates, use of compositions comprising such enzymes in cleaning processes.
Type:
Application
Filed:
October 9, 2019
Publication date:
February 3, 2022
Applicant:
Novozymes A/S
Inventors:
Klaus Gori, Lorena Gonzalez Palmen, Jesper Salomon, Kenneth Jensen
Abstract: The present invention relates to cleaning compositions comprising polypeptides having peptidoglycan degradation activity, as well as use of the cleaning compositions for cleaning of an item such as a textile or a surface.
Type:
Application
Filed:
December 19, 2019
Publication date:
February 3, 2022
Applicant:
Novozymes A/S
Inventors:
Christian Berg Oehlenschlaeger, Dorotea Raventos Segura, Jesper Salomon, Fabian Barrientos Garcia, Lillian Eva Tang Baltsen, Christian Bech Rosen
Abstract: The present invention relates to polypeptides having nuclease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and recombinant host cells comprising the polynucleotides as well as methods of producing, recovering and using the polypeptides. The invention further relates to cleaning compositions comprising one or more of the polypeptides having nuclease activity, as well as a cleaning method and use of the polypeptides.
Type:
Application
Filed:
October 3, 2019
Publication date:
February 3, 2022
Applicant:
Novozymes A/S
Inventors:
Tianqi Sun, Jesper Salomon, Klaus Gori, Morten Gjermansen, Marc Dominique Morant, Mary Ann Stringer