Patents Assigned to Novozymes Biotech, Inc.
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Patent number: 6686189Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: June 12, 2002Date of Patent: February 3, 2004Assignee: Novozymes Biotech, Inc.Inventors: Michael W. Rey, Elizabeth J. Golightly
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Patent number: 6682922Abstract: The present invention relates to isolated polypeptides having lysophospholipase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: October 2, 2002Date of Patent: January 27, 2004Assignees: Novozymes Biotech, Inc., Novozymes Bioindustry, Ltd.Inventors: Randy M. Berka, Michael W. Rey, Tony Byun, Ryoko Itami, Noriko Tsutsumi, Alan Klotz
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Publication number: 20040009579Abstract: The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.Type: ApplicationFiled: February 25, 2003Publication date: January 15, 2004Applicant: Novozymes Biotech, Inc.Inventors: Jeffrey R. Shuster, John C. Royer
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Patent number: 6673571Abstract: The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: September 19, 2001Date of Patent: January 6, 2004Assignee: Novozymes Biotech, Inc.Inventors: Alexander Blinkovsky, Tony S. Byun, Alan V. Klotz, Alan Sloma, Kimberly Brown, Maria Tang, Mikio Fujii, Chigusa Marumoto, Lene Venke Kofod
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Patent number: 6667169Abstract: The present invention relates to isolated polypeptides having acid phosphatase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: September 19, 2001Date of Patent: December 23, 2003Assignee: Novozymes Biotech, Inc.Inventors: Debbie S. Yaver, Randy M. Berka, Michael W. Rey
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Patent number: 6664092Abstract: The present invention relates to isolated polypeptides having dipeptidyl aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: May 15, 1998Date of Patent: December 16, 2003Assignee: Novozymes Biotech, Inc.Inventors: Alexander Blinkovsky, Kimberly Brown, Michael W. Rey, Alan Klotz, Tony Byun
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Publication number: 20030199037Abstract: The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous fungal host cells a first nucleic acid sequence encoding a recombination protein and a second nucleic acid sequence comprising one or more regions which are homologous with the genome of the filamentous fungal host cell, wherein (i) the recombination protein promotes the recombination of the one or more regions with the corresponding homologous region in the host's genome to incorporate the second nucleic acid sequence by homologous recombination, and (ii) the number of host cells comprising the incorporated second nucleic acid sequence in the population is increased at least 20% compared to the same population without the first nucleic acid sequence; (b) and isolating from the population a filamentous fungal cell comprising the incorporated second nucleic acid sequence.Type: ApplicationFiled: April 21, 2003Publication date: October 23, 2003Applicant: Novozymes Biotech, Inc.Inventors: Paul Harris, Howard Brody
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Publication number: 20030199038Abstract: The present invention relates to methods for preparing variants of a nucleotide sequence, comprising: (a) introducing into a population of filamentous fungal host cells: (i) one or more circular plasmids comprising a DNA sequence and a plasmid replicator mediating autonomous replication, wherein the one or more circularized plasmids are linearized by digestion of the DNA sequence and removal of a portion of the DNA sequence; and (ii) a library of DNA fragments comprising one or more mutations of the DNA sequence, wherein the fragments comprise at least two regions, one or more regions which are homologous to the 5′ region or the 3′ region of the gap in the linearized DNA sequence and/or plasmid sequence and one or more second regions which are homologous to the 5′ region or the 3′ region of the DNA fragments of the library; wherein the linearized plasmids and the DNA fragments recombine by in vivo recombination to produce a plurality of autonomously replicating plasmids comprising oneType: ApplicationFiled: April 22, 2003Publication date: October 23, 2003Applicant: Novozymes Biotech, Inc.Inventors: Howard Brody, Suzanne M. Otani, Joel R. Cherry
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Publication number: 20030186380Abstract: The present invention relates to recombinant methods for producing a secreted polypeptide having L-asparaginase activity, comprising (a) cultivating under conditions conducive for production of the polypeptide a host cell comprising a nucleic acid construct comprising a first nucleic acid sequence encoding a secretory signal peptide operably linked to second nucleic acid sequence encoding the polypeptide having L-asparaginase activity, wherein the signal peptide directs the polypeptide into the cell's secretory pathway; and (b) recovering the secreted polypeptide. The present invention also relates to isolated polypeptides having L-asparaginase activity and nucleic acids thereof.Type: ApplicationFiled: April 1, 2003Publication date: October 2, 2003Applicant: Novozymes Biotech, Inc.Inventors: Michael D. Thomas, Alan Sloma
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Publication number: 20030180418Abstract: The present invention relates to methods for preparing a dough, comprising incorporating into the dough a composition comprising an effective amount of a lipase which improves one or more properties of the dough or a baked product obtained from the dough. The present invention also relates to methods for preparing a baked product. The present invention also relates to compositions comprising an effective amount of such a lipase for improving one or more properties of a dough and/or a baked product obtained from the dough. The present invention further relates to doughs or baked products and to pre-mixes for a dough.Type: ApplicationFiled: May 5, 2003Publication date: September 25, 2003Applicants: Novozymes Biotech, Inc., Novozymes A/SInventors: Michael W. Rey, Elizabeth J. Golightly, Tina Spendler
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Publication number: 20030175902Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: ApplicationFiled: December 20, 2002Publication date: September 18, 2003Applicant: Novozymes Biotech, Inc.Inventors: Alan Sloma, Regine Behr, William Widner, Maria Tang, David Sternberg, Stephen Brown
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Publication number: 20030170876Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.Type: ApplicationFiled: April 12, 2001Publication date: September 11, 2003Applicant: Novozymes Biotech, Inc.Inventors: William Widner, Alan Sloma, Michael D. Thomas
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Publication number: 20030148452Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the nucleic acid sequence, wherein the promoter comprises a sequence selected from the group consisting of nucleotides 1 to 3949 of SEQ ID NO. 1, nucleotides 1 to 938 of SEQ ID NO. 2, and nucleotides 1 to 3060 of SEQ ID NO. 3, and a subsequence thereof; and mutant, hybrid, and tandem promoters thereof; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated promoter sequences and to constructs, vectors, and fungal host cells comprising the promoter sequences operably linked to nucleic acid sequences encoding polypeptides.Type: ApplicationFiled: October 28, 2002Publication date: August 7, 2003Applicant: Novozymes Biotech, Inc.Inventors: Randy M. Berka, Michael W. Rey, Kimberly Brown, Stephen H. Brown
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Patent number: 6586215Abstract: The present invention relates to isolated polypeptides having peroxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: June 19, 2001Date of Patent: July 1, 2003Assignee: Novozymes Biotech, Inc.Inventors: Debbie Yaver, Barbara McArdle
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Publication number: 20030100092Abstract: The present invention relates to isolated polypeptides having lysophospholipase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: ApplicationFiled: October 2, 2002Publication date: May 29, 2003Applicant: Novozymes Biotech, Inc.Inventors: Randy M. Berka, Michael W. Rey, Tony Byun, Ryoko Itami, Noriko Tsutsumi, Alan Klotz
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Patent number: 6558715Abstract: The present invention relates to methods for preparing a dough, comprising incorporating into the dough a composition comprising an effective amount of a lipase which improves one or more properties of the dough or a baked product obtained from the dough. The present invention also relates to methods for preparing a baked product. The present invention also relates to compositions comprising an effective amount of such a lipase for improving one or more properties of a dough and/or a baked product obtained from the dough. The present invention further relates to doughs or baked products and to pre-mixes for a dough.Type: GrantFiled: October 31, 2000Date of Patent: May 6, 2003Assignees: Novozymes Biotech, Inc., Novozymes A/SInventors: Michael W. Rey, Elizabeth J. Golightly, Tina Spendler
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Patent number: 6555147Abstract: The present invention relates to methods for making enzyme-treated unpopped popcorn kernels, comprising: (a) contacting the popcorn kernels with an aqueous solution comprising an effective amount of one or more hull wall-degrading enzymes for a period of time sufficient to weaken the hull wall of the kernels; and (b) recovering the enzyme-treated popcorn kernels. The present invention also relates to popcorn kernels obtained by the methods of the present invention.Type: GrantFiled: August 7, 2000Date of Patent: April 29, 2003Assignee: Novozymes Biotech, Inc.Inventor: Feng Xu
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Publication number: 20030078393Abstract: The present invention relates to methods for producing a coagulated whey protein, comprising: (a) partially hydrolyzing a whey protein with a glutamate-aspartate specific endoprotease in an aqueous solution, under conditions that coagulates a portion of the water-soluble whey protein; and (b) recovering the coagulated whey protein, wherein the coagulated whey protein exhibits neutral organoleptic properties. The present invention also relates to coagulated whey protein and to food products that comprise such coagulated whey protein.Type: ApplicationFiled: September 13, 2002Publication date: April 24, 2003Applicant: Novozymes Biotech, Inc.Inventors: Alexander Blinkovsky, Tony Byun
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Patent number: 6548274Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide; and (b) isolating the polypeptide from the cultivation medium; wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide in tandem with a second nucleic acid sequence comprising a crippled translational initiator sequence operably linked to a gene encoding a selectable marker, wherein the copy number of the first nucleic acid sequence has been increased by culturing the cell under conditions that select for multiple copies of the selectable marker. The present invention also relates to such fungal host cells and methods for obtaining such fungal host cells. The present invention further relates to nucleic acid constructs and vectors comprising a crippled translational initiator sequence operably linked to a gene encoding a selectable marker.Type: GrantFiled: January 12, 2001Date of Patent: April 15, 2003Assignee: Novozymes Biotech, Inc.Inventors: Debbie S. Yaver, Daniel A. Bellini
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Patent number: 6544774Abstract: The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.Type: GrantFiled: November 2, 2000Date of Patent: April 8, 2003Assignee: Novozymes Biotech, Inc.Inventors: Jeffrey R. Shuster, John C. Royer