Patents Assigned to Novozymes Biotech, Inc.
  • Publication number: 20030044949
    Abstract: The present invention relates to isolated polypeptides having gamma-glutamyl transpeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Application
    Filed: March 27, 2002
    Publication date: March 6, 2003
    Applicant: Novozymes Biotech, Inc.
    Inventors: Regine Behr, Alan Sloma
  • Publication number: 20030040450
    Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Application
    Filed: June 12, 2002
    Publication date: February 27, 2003
    Applicant: Novozymes Biotech, Inc.
    Inventors: Michael W. Rey, Elizabeth J. Golightly
  • Patent number: 6521435
    Abstract: The invention is directed to nucleic acid sequences derived from Fusarium fungal genes which encode the cell wall-degrading enzymes glucanase, endochitinase, and exochitinase; isolated polypeptides having glucanase, endochitinase or exochitinase activity; recombinant nucleic acid molecules, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in plant cells to confer or enhance a plant's resistance to Fusarium and other pathogens.
    Type: Grant
    Filed: August 28, 2000
    Date of Patent: February 18, 2003
    Assignees: The United States of America as represented by the Secretary of Agriculture, Novozymes Biotech, Inc.
    Inventors: Patricia A. Okubara, Ann E. Blechl, Thomas M. Hohn, Randy M. Berka
  • Patent number: 6518044
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the nucleic acid sequence, wherein the promoter comprises a sequence selected from the group consisting of nucleotides 1 to 3949 of SEQ ID NO:1, nucleotides 1 to 938 of SEQ ID NO:2, and nucleotides 1 to 3060 of SEQ ID NO:3, and a subsequence thereof; and mutant, hybrid, and tandem promoters thereof; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated promoter sequences and to constructs, vectors, and fungal host cells comprising the promoter sequences operably linked to nucleic acid sequences encoding polypeptides.
    Type: Grant
    Filed: October 30, 2001
    Date of Patent: February 11, 2003
    Assignee: Novozymes Biotech, Inc.
    Inventors: Randy M. Berka, Michael W. Rey, Kimberly Brown, Stephen H. Brown
  • Publication number: 20030027310
    Abstract: The present invention relates to isolated polypeptides having lactonohydrolase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides. The present invention further relates to methods for preventing microbial biofilm development.
    Type: Application
    Filed: April 19, 2002
    Publication date: February 6, 2003
    Applicant: Novozymes Biotech, Inc.
    Inventors: Randy M. Berka, Michael W. Rey
  • Publication number: 20030013182
    Abstract: The present invention relates to methods for isolating a gene encoding an enzyme, comprising: (a) adding a mixture of labeled first nucleic acid probes from a microbial strain cultured on medium without the substrate, and labeled second nucleic acid probes from a microbial strain cultured on medium with the substrate, to an array of random nucleic acid fragments of the microbial strain where the labeled nucleic acids hybridize to complementary sequences of the genomic fragments in the array, wherein the first nucleic acid probes are labeled with a first reporter and the second nucleic acid probes are labeled with a second reporter; (b) examining the array under conditions wherein the relative expression of the genes of the microbial strain is determined by the observed hybridization reporter signal of each spot in the array; and (c) isolating a gene from the microbial strain that encodes an enzyme that degrades the substrate. The present invention also relates to isolated genes obtained by such methods.
    Type: Application
    Filed: March 12, 2002
    Publication date: January 16, 2003
    Applicant: Novozymes Biotech, Inc.
    Inventors: Debbie S. Yaver, Randy M. Berka
  • Patent number: 6489154
    Abstract: The present invention relates to isolated polypeptides having lysophospholipase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: November 10, 1999
    Date of Patent: December 3, 2002
    Assignees: Novozymes Biotech, Inc., Novozymes Bioindustry, Ltd.
    Inventors: Randy M. Berka, Michael W. Rey, Tony Byun, Ryoko Itami, Noriko Tsutsumi, Alan Klotz
  • Publication number: 20020177210
    Abstract: The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Application
    Filed: September 19, 2001
    Publication date: November 28, 2002
    Applicant: Novozymes Biotech, Inc.
    Inventors: Alexander Blinkovsky, Tony S. Byun, Alan V. Klotz, Alan Sloma, Kimberly Brown, Maria Tang, Mikio Fujii, Chigusa Marumoto, Lene Venke Kofod
  • Patent number: 6485761
    Abstract: The present invention relates to methods for preparing a dough, comprising incorporating into the dough a composition comprising an effective amount of one or more lactonohydrolases which improve one or more properties of the dough or a baked product obtained from the dough. The present invention also relates to methods for preparing a baked product. The present invention also relates to compositions comprising an effective amount of one or more lactonohydrolases for improving one or more properties of a dough and/or a baked product obtained from the dough. The present invention further relates to doughs or baked products and to pre-mixes for a dough.
    Type: Grant
    Filed: February 24, 2000
    Date of Patent: November 26, 2002
    Assignee: Novozymes Biotech, Inc.
    Inventor: Feng Xu
  • Publication number: 20020164753
    Abstract: The present invention relates to isolated polypeptides having acid phosphatase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Application
    Filed: September 19, 2001
    Publication date: November 7, 2002
    Applicant: Novozymes Biotech, Inc.
    Inventors: Debbie S. Yaver, Randy M. Berka, Michael W. Rey
  • Patent number: 6465209
    Abstract: The present invention relates to methods of producing protein hydrolysates, comprising adding to a proteinaceous material one or more aminopeptidase(s) having glycine releasing properties and one or more additional proteases wherein the amount of glycine produced is greater than the amount of glycine produced by the one or more additional proteases alone under the same conditions.
    Type: Grant
    Filed: May 15, 1998
    Date of Patent: October 15, 2002
    Assignees: Novozymes Biotech, Inc., Novozymes A/S, Japan Tobacco, Inc.
    Inventors: Alexander Blinkovsky, Kimberly Brown, Elizabeth Golightly, Tony Byun, Thomas E. Mathiasen, Lene V. Kofod, Mikio Fujii, Chigusa Marumoto
  • Publication number: 20020146721
    Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.
    Type: Application
    Filed: October 5, 2001
    Publication date: October 10, 2002
    Applicant: Novozymes Biotech, Inc.
    Inventors: Randy M. Berka, Ib Groth Clausen
  • Patent number: 6461837
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a consensus translational initiator sequence foreign to the nucleic acid sequence; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated consensus translational initiator sequences and to constructs, vectors, and fungal host cells comprising the consensus translational initiator sequences operably linked to nucleic acid sequences encoding polypeptides.
    Type: Grant
    Filed: November 20, 2000
    Date of Patent: October 8, 2002
    Assignee: Novozymes Biotech, Inc.
    Inventors: Debbie S. Yaver, Daniel Alan Bellini
  • Patent number: 6432898
    Abstract: The present invention relates to isolated polypeptides having lipase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: October 31, 2000
    Date of Patent: August 13, 2002
    Assignee: Novozymes Biotech, Inc.
    Inventors: Michael W. Rey, Elizabeth J. Golightly
  • Patent number: 6395529
    Abstract: The present invention relates to isolated polypeptides having lactonohydrolase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides. The present invention further relates to methods for preventing microbial biofilm development.
    Type: Grant
    Filed: November 5, 1999
    Date of Patent: May 28, 2002
    Assignee: Novozymes Biotech, Inc.
    Inventors: Randy M. Berka, Michael W. Rey
  • Patent number: 6372464
    Abstract: The present invention relates to isolated polypeptides having peroxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: June 19, 2000
    Date of Patent: April 16, 2002
    Assignee: Novozymes Biotech, Inc.
    Inventors: Debbie S. Yaver, Barbara McArdle
  • Patent number: 6361973
    Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the nucleic acid sequence, wherein the promoter comprises a sequence selected from the group consisting of nucleotides 1 to 3949 of SEQ ID NO:1, nucleotides 1 to 938 of SEQ ID NO:2, and nucleotides 1 to 3060 of SEQ ID NO:3, and a subsequence thereof; and mutant, hybrid, and tandem promoters thereof; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated promoter sequences and to constructs, vectors, and fungal host cells comprising the promoter sequences operably linked to nucleic acid sequences encoding polypeptides.
    Type: Grant
    Filed: March 22, 2000
    Date of Patent: March 26, 2002
    Assignee: Novozymes Biotech, Inc.
    Inventors: Randy M. Berka, Michael W. Rey, Kimberly Brown, Stephen H. Brown
  • Publication number: 20020004085
    Abstract: The present invention relates to methods for producing consumable products from potatoes, comprising: (a) treating a potato substance with an effective amount of one or more exogenous enzymes selected from the group consisting of an amyloglucosidase, glucose oxidase, laccase, lipase, maltogenic amylase, pectinase, pentosanase, protease, and transglutaminase, and (b) processing the enzyme-treated potato substance to produce a potato product. The invention also relates to consumable products obtained from potatoes by the methods of the present invention.
    Type: Application
    Filed: April 13, 2001
    Publication date: January 10, 2002
    Applicant: Novozymes Biotech, Inc.
    Inventors: Feng Xu, Lene Venke Kofod, Hans Sejr Olsen
  • Patent number: 6323002
    Abstract: The present invention relates to methods for modifying the production of a polypeptide, comprising: (a) introducing a nucleic acid construct into a cell, wherein the cell comprises a DNA sequence encoding a polypeptide, under conditions in which the nucleic acid construct integrates into the genome of the cell at a locus not within the DNA sequence encoding the polypeptide to produce a mutant cell, wherein the integration of the nucleic acid construct modifies the production of the polypeptide by the mutant cell relative to the cell when the mutant cell and the cell are cultured under the same conditions; and (b) identifying the mutant cell with the modified production of the polypeptide.
    Type: Grant
    Filed: June 25, 1999
    Date of Patent: November 27, 2001
    Assignee: Novozymes Biotech, Inc
    Inventors: Howard Brody, Deborah S. Yaver, Michael Lamsa, Kim Hansen
  • Patent number: 6320103
    Abstract: The present invention relates to isolated polypeptides having choline oxidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: October 12, 2000
    Date of Patent: November 20, 2001
    Assignee: Novozymes Biotech, Inc.
    Inventors: Debbie Yaver, Randy M. Berka, Michael W. Rey