Abstract: Strategically truncated probes specific for high-risk HPV nucleic acids, and methods for making and using the same, are disclosed herein. The disclosed probes, and methods of use thereof, permit fast and reliable detection of human papillomavirus in clinical samples without significant cross-reaction.
Abstract: Methods are provided for genotyping a target nucleic acid in a sample. In various aspects, the methods comprise generating nucleic acid hybrids between probes specific for the genotypes of interest and the target nucleic acid and detecting hybridization in the sample. In other aspects, the methods comprise using multi-probe mixtures to reduce the volume of sample necessary to determine the genotype of the target nucleic acid.
Abstract: The present disclosure provides a method for coordinating data between a plurality of sample processing systems comprising: providing, using a central control unit, data to a pre-analytical sample processing system identifying a sample undergoing pre-analytical processing, receiving data, at the central control unit, indicating the transfer of the sample to an analytical system, providing, from the central control unit (CCU), data associated with the sample to the analytical system, and receiving a result associated with the sample from the analytical system
Type:
Application
Filed:
November 18, 2010
Publication date:
October 6, 2011
Applicant:
Qiagen Gaithersburg, Inc.
Inventors:
John SCOTT, Lauren Hannon, Jason Troxell, Emmet Brown
Abstract: Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step using two separate sequence-specific polynucleotide probes. Also provided are nucleic acids comprising SEQ ID NO: 1 to SEQ ID NO: 727 and nucleic acid probes and probe sets comprising the same.
Abstract: Methods and materials for improving nucleic acid or protein recovery from samples preserved in liquid cytological preservative solutions by utilizing scavenging agents, such as hydrazine- and hydrazide-containing compounds, are provided. Lysis solutions comprising hydrazine- and hydrazide-containing compounds and kits comprising the same are also provided.
Type:
Application
Filed:
January 4, 2011
Publication date:
August 11, 2011
Applicant:
QIAGEN GAITHERSBURG INC.
Inventors:
Yuri KHRIPIN, Arvind Virmani, Lori Kobayashi
Abstract: A system and method for automated handling of vials containing liquid medical specimens is disclosed. The robotic system processes the specimens for further downstream molecular analysis. The processing comprises automated vial cap removal, pipetting of the vial contents, transfer of the vial contents to a destination tray such as a multiwell plate, and recapping of the vial.
Type:
Grant
Filed:
April 4, 2008
Date of Patent:
July 26, 2011
Assignee:
Qiagen Gaithersburg, Inc.
Inventors:
Ted Carl Edens, Richard Obiso, Gerard J. Sevigny, Michael Touma, Mark Messina, Brian McKeen, Robert C. Aviles
Abstract: This invention provides novel methods for assessing HPV infection. Gene expression levels are used to assess the progression of HPV infection from benign to malignant growth. Also provided are kits for carrying out the methods of this invention.
Abstract: The present disclosure relates to methods for isolating, amplifying, and/or analyzing nucleic acids in the presence of an anion exchange material by performing the isolation, amplification and/or analysis step in the presence of at least one anionic compound.
Type:
Application
Filed:
August 5, 2010
Publication date:
March 24, 2011
Applicants:
QIAGEN GAITHERSBURG INC., QIAGEN GMBH
Inventors:
Yuri KHRIPIN, Dirk Loeffert, Roland Fabis, Nadine Krueger
Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA-RNA hybrids which can be detected by a variety of methods.
Type:
Application
Filed:
October 14, 2010
Publication date:
March 10, 2011
Applicant:
QIAGEN GAITHERSBURG INC.
Inventors:
James Anthony, Attila Lorincz, John Troy, Yanglin Tan
Abstract: Methods and kits for determining load of an infectious agent in a sample are described, comprising performing at least one hybridization assay and calculating the load of the infectious agent in the sample from a detected nucleic acid. In particular, the methods and kits disclosed determine the load of human papillomavirus (HPV) in a sample.
Type:
Application
Filed:
September 3, 2010
Publication date:
March 3, 2011
Applicant:
QIAGEN GAITHERSBURG INC.
Inventors:
Brian Lowe, Dominic O'Neil, Irina Nazarenko, Dirk Loeffert
Abstract: This invention provides methods for assessing HPV infection. Gene expression levels are used to assess the progression of HPV infection from benign to malignant growth. Also provided are kits for carrying out the methods of this invention.
Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but also highly specific and capable of discriminating highly homologous nucleic acid sequences. The method produces DNA/RNA hybrids which can be detected by a variety of methods.
Type:
Application
Filed:
November 19, 2009
Publication date:
January 6, 2011
Applicant:
QIAGEN GAITHERSBURG INC.
Inventors:
James ANTHONY, Attila Lorinez, Inna Williams, John Troy, Yanlin Tang
Abstract: Provided are methods of isolating RNA from a biological sample, methods and means for determining the presence of particular RNA splice-form variants in a biological sample, methods and means for determining the relative ratio of RNA ratios in a biological sample, and methods and means for predicting the progression of precancerous cervical lesions.
Type:
Application
Filed:
April 30, 2010
Publication date:
December 9, 2010
Applicant:
QIAGEN GAITHERSBURG INC.
Inventors:
Brian Lowe, Anna K. Fulbright, Irina Nazarenko
Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA-RNA hybrids which can be detected by a variety of methods.
Type:
Grant
Filed:
October 19, 2008
Date of Patent:
November 9, 2010
Assignee:
Qiagen Gaithersburg, Inc.
Inventors:
James Anthony, Attila Lorincz, John Troy, Yanglin Tan
Abstract: Methods of selectively and rapidly identifying target nucleic acid molecules in large volumes of collection media where the target is present in a low concentration are disclosed. The methods can be used to identify, isolate, purify, or enrich a nucleic acid molecule containing a specific target sequence from a sample of nucleic acid molecules that do not contain the specific target sequence. Once isolated, the nucleic acid molecule containing a specific target sequence may be amplified or used in a variety of detection assays.
Abstract: The present invention comprises a method that provides fast and reliable results for detecting the presence of a target nucleic acid molecule in a sample.
Type:
Application
Filed:
October 26, 2009
Publication date:
June 24, 2010
Applicant:
QIAGEN GAITHERSBURG INC.
Inventors:
Paul Eder, Eric Payne, Irina Nazarenko, Suganthi Ramachandran, Arvind Virmani, Laura Bell
Abstract: The present invention comprises a method that provides fast and reliable results for detecting the presence of a target nucleic acid molecule in a sample.
Type:
Application
Filed:
October 26, 2009
Publication date:
April 29, 2010
Applicant:
QIAGEN GAITHERSBURG INC.
Inventors:
Paul EDER, Eric PAYNE, Irina NAZARENKO, Suganthi Ramachandran, Arvind VIRMANI, Laura BELL
Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA:RNA hybrids which can be detected by a variety of methods.
Type:
Application
Filed:
October 9, 2009
Publication date:
February 11, 2010
Applicant:
QIAGEN GAITHERSBURG INC.
Inventors:
Irina Nazarenko, Attila Lorincz, Paul Eder, Brian Lowe, Richard Mallonee, Ha Thai
Abstract: Target-specific hybrid capture (TSHC) provides a nucleic acid detection method that is not only rapid and sensitive, but is also highly specific and capable of discriminating highly homologous nucleic acid target sequences. The method produces DNA/RNA hybrids which can be detected by a variety of methods.
Type:
Grant
Filed:
June 15, 2001
Date of Patent:
January 12, 2010
Assignee:
Qiagen Gaithersburg, Inc.
Inventors:
James Anthony, Attila Lorincz, Inna Williams, John Troy, Yanlin Tang