Patents Assigned to QIAGEN GmbH
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Patent number: 9097628Abstract: The present invention relates to a method for processing a wax-embedded biological sample, the use of poly(organosiloxane)s for liquefying the embedding medium of a wax-embedded biological sample and a kit for processing a wax-embedded biological sample.Type: GrantFiled: December 23, 2011Date of Patent: August 4, 2015Assignee: QIAGEN, GMbHInventor: Martin Schlumpberger
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Publication number: 20150204843Abstract: A method for obtaining blood plasma from a whole blood sample comprising the following steps a) contacting the whole blood sample with a composition (A) comprising at least one carboxylic acid, wherein the addition of the acidic composition (A) and optionally further additives to the whole blood sample provides a sample mixture having a pH that lies in a range from 2.5 to 5; b) binding red and white blood cells to a magnetic solid phase; wherein step a) and step b) can be performed sequentially or simultaneously, c) separating the solid phase with the bound cells from the remaining sample thereby providing blood plasma.Type: ApplicationFiled: August 29, 2013Publication date: July 23, 2015Applicant: QIAGEN GmbHInventors: Andy Wende, Sabine Werner, Ralf Himmelreich
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Patent number: 9074248Abstract: It has been unexpectedly and surprisingly found that Helicase Dependent Amplification (HDA) primers having termini enriched for A or C at the 5?-ends, result in a much more efficient HDA reaction than those primers having G or T rich 5?-ends. Since A is a low melting base and C is a high-melting base, the melting characteristic of primer termini is not correlated with melting characteristics of amplicon termini. Optimized HDA primers, methods of making and using optimized primers, as well as methods of as well as kits for optimized HDA are disclosed.Type: GrantFiled: December 18, 2012Date of Patent: July 7, 2015Assignee: QIAGEN GMBHInventors: Christian Korfhage, Sven van Ooyen
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Patent number: 9040243Abstract: The present invention relates to a method, kit and use of various nucleic acid sequences for deleting and/or quantifying one or more nucleic acids of a genome in a sample. Wherein the nucleic acid is amplified and the locus that is amplified is a multi copy locus within the genome, the multicopy locus has copies on at least two different chromosomes and the amplification product is detected and/or quantified.Type: GrantFiled: September 22, 2011Date of Patent: May 26, 2015Assignee: QIAGEN GMBHInventors: Andy Wende, Francesca Dipasquale, Sabine Werner, Sascha Strauss
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Patent number: 9017557Abstract: The present invention concerns new monolithic shaped bodies, a method for their preparation as well as their use especially for the selective purification and separation of biopolymers. A chromatographic separation material is provided with the new monolithic shaped bodies that allows a selective, efficient and reproducible purification and separation of biopolymers.Type: GrantFiled: July 27, 2005Date of Patent: April 28, 2015Assignee: Qiagen GmbHInventors: Christoph Erbacher, Christoph Ritt, Markus Kirchmann
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Patent number: 9012146Abstract: The present invention relates to a method for selectively enriching and/or isolating microbial and optionally additionally viral nucleic acids from samples that contain a mixture of microbial cells, freely circulating nucleic acids and higher eukaryotic cells, and optionally additionally viruses, in a liquid, and to a kit for selectively enriching and/or isolating intracellular and extracellular microbial nucleic acids, and optionally additionally viral nucleic acids, from samples that contain a mixture of microbial and higher eukaryotic cells, freely circulating nucleic acids, in particular extracellular microbial nucleic acids, and optionally additionally viruses in a liquid.Type: GrantFiled: November 19, 2010Date of Patent: April 21, 2015Assignee: QIAGEN GmbHInventors: Dirk Heckel, Thomas Doedt
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Patent number: 9005933Abstract: The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.Type: GrantFiled: August 16, 2011Date of Patent: April 14, 2015Assignee: Qiagen GmbHInventors: Christian Korfhage, Gerd Grosshauser, Thomas Rothmann, Ralf Himmelreich
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Patent number: 8997800Abstract: A system (10) for removing a pipettable substance from a pre-filled container (20), which is closed off by a top (30) having at least one opening area (40), comprises an opening tool (100) having a tube (110), which has a cross-section corresponding substantially to the shape of the opening area and which comprises at a distal end (120) an endpiece (140) extending substantially obliquely relative to the longitudinal axis of the tube, which moves a part of the top (30) located inside the opening area (40) towards the container when the opening tool is applied, so as to form an opening in the top, and a point of attack (150) for a transporting tool (200). The opening tool (100) is designed to remain on the container (20) after use.Type: GrantFiled: August 18, 2008Date of Patent: April 7, 2015Assignee: Qiagen GmbHInventors: Thomas Voit, Andrea Wildhaber
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Patent number: 8980552Abstract: The invention describes a method of and kits for isolating and/or purifying nucleic acids, more specifically short-chain nucleic acids such as miRNA, from a nucleic acid-containing starting material, characterized by the following method steps of: (a) binding the nucleic acids to a nucleic acid-binding support material by contacting the starting material with said nucleic acid-binding support material in the presence of at least one chaotropic compound, at least two different detergents and at least one branched and/or unbranched alcohol, preferably isopropanol, with the concentration of said alcohol being 40% (v/v); (b) optionally eluting the bound nucleic acids from the nucleic acid-binding support material. The method of the invention is particularly suitable for purifying circulating, extracellular miRNA from blood.Type: GrantFiled: June 21, 2010Date of Patent: March 17, 2015Assignee: QIAGEN GmbHInventors: Martin Horlitz, Markus Sprenger-Haussels
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Patent number: 8962250Abstract: The invention relates to improved methods of amplifying and optionally quantifying and/or identifying a plurality of selected nucleic acid molecules from a pool of nucleic acid molecules. A first round of multiplex amplification used where the amplification reaction is allowed to proceed to a point prior to that at which significant competition between amplicons for reaction components has occurred. This is the followed by a second round of amplification that typically includes a fluorescent reporter to allow for each of the selected nucleic acid sequences to be quantified. The methods are useful for the amplification and quantification of nucleic acids from a variety of sources, such as gene expression products, whereby many such products may be amplified and quantified from very limited samples and from degraded archival samples.Type: GrantFiled: September 1, 2006Date of Patent: February 24, 2015Assignee: Qiagen GmbHInventor: Keith Stanley
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Patent number: 8962820Abstract: The present invention relates to combinations of fluorescent dyes used in molecular biology, particularly in multiplex PCR. In particular, the present invention relates to a combination of dyes for amplification reactions, wherein at least four different dyes are used, wherein the first dye is 5-FAM or 6-FAM or a blend thereof, the second dye is selected from the group consisting of DY-530, HEX, CAL Fluor Orange 560 and ATTO 532, the third dye is selected from the group consisting of ATTO 550, DY-555 and DY-556, the fourth dye is selected from the group consisting of ROX, DY-510XL and ATTO 565, and optionally a fifth dye is selected from the group consisting of DY 632 and DY-520XL.Type: GrantFiled: November 27, 2013Date of Patent: February 24, 2015Assignee: Qiagen GmbHInventors: Werner Brabetz, Cornelia Weber
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Patent number: 8940484Abstract: The invention provides allelic ladder mixtures and individual alleles suitable for use in such mixtures. The allelic ladder mixtures give improved identification and distinguishing capabilities, particularly suitable in forensic investigations.Type: GrantFiled: February 7, 2013Date of Patent: January 27, 2015Assignee: Qiagen GmbHInventors: Rebecca A. L. Barber, Michael D. Barber, Peter E. Johnson, Sharon M. Gillbard, Marc D. Haywood, Carolyn D. Smith, Jennifer A. Arnold, Trudy Burke, Andrew J. Urquhart, Peter P. Gill
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Patent number: 8937174Abstract: The invention relates to a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further processed by a microfluidic system. A container is provided with a filter and can be or is closed. A biological sample is introduced into the container. An inlet or outlet for liquids is mounted downstream of the filter. In order to carry out processing, liquids that are present in the container are not only sucked off through the filter and passed on via the inlet or outlet, but liquids required for processing are also pumped into the container through the filter. The container can be connected to a microfluidic system in a relatively easy manner since only one conduit is required for an automated processing.Type: GrantFiled: January 13, 2009Date of Patent: January 20, 2015Assignee: Qiagen GmbHInventors: Thomas Rothmann, Ralf Himmelreich, Thomas Laue
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Patent number: 8936909Abstract: A method for determining the nature of a sample is provided, wherein the presence or absence of at least one marker small non-coding RNA in the sample is detected. Suitable marker small non-coding RNAs for different samples such as blood, saliva, semen and vaginal secretions are provided. Also provided are suitable kits and methods for identifying marker small-non coding RNAs.Type: GrantFiled: July 20, 2009Date of Patent: January 20, 2015Assignees: Qiagen GmbH, University of Central Florida Research Foundation Inc.Inventors: Helge Lubenow, Jack Ballantyne, Erin K. Hanson
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Patent number: 8932542Abstract: A gripper unit for handling a vessel for receiving biological material is proposed, inter alia. The vessel has a lid which can assume an open position and a closed position. The gripper unit comprises a gripper for gripping and releasing the vessel, and a lid holder, for holding a lid in a defined position in relation to the vessel. The defined position is an open position of the lid.Type: GrantFiled: September 26, 2006Date of Patent: January 13, 2015Assignee: Qiagen GmbHInventors: Andreas Schaefer, Thomas Voit, Walter Tschopp, Adrian Geiger, Markus Zbinden, Harald Hibbing, Andreas Karl, Frank Eigemeier, Volker Behrmann, Dietmar Kopp, Andreas Schmiede
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Patent number: 8932831Abstract: The present invention concerns a method for inserting one or more tag sequences into a nucleic acid characterized by the following steps: (a) preparation of a template nucleic acid; (b) hybridization of at least one anchor sequence of at least one anchor oligonucleotide with one sequence section of the template nucleic acid; and (c) synthesization of a new strand of nucleic acid, which is partially complementary to the template nucleic acid and which contains a sequence complementary to the non-hybridized portion of the anchor oligonucleotide, e.g. to at least one tag sequence, on its 3? end.Type: GrantFiled: May 4, 2007Date of Patent: January 13, 2015Assignee: Qiagen GmbHInventors: Christian Korfhage, Holger Engel, Dirk Löffert, Ralf Peist
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Publication number: 20140356860Abstract: The present invention provides a method for isolating nucleic acids from a veterinary whole blood sample, said method comprising at least the following steps a) preparing a binding mixture comprising—the lysed sample—at least one chaotropic agent—at least one alcohol—at least one polyoxyethylene fatty alcohol ether; b) passing the binding mixture through a column comprising a nucleic acid binding solid phase thereby binding the nucleic acids to the nucleic acid binding solid phase; c) optionally washing the nucleic acids while being bound to the solid phase; d) optionally eluting the nucleic acids from the solid phase. It was found that the addition of the specific non-ionic detergent overcomes the problems of the prior art methods, wherein the column clogs what prevents the efficient nucleic acid isolation from this difficult sample.Type: ApplicationFiled: September 13, 2011Publication date: December 4, 2014Applicant: QIAGEN GMBHInventors: Denis Flügge, Lillian Krüger, Mario Scherer
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Patent number: 8887754Abstract: The invention relates to a system comprising a rotary valve and an actuating device for the rotary valve. The invention further relates to a related flow system having a rotary valve. The flow system is in particular a micro-flow system. The system comprises at least two separate components, wherein the one component is a rotary valve comprising a hollow cylindrical mount (2), a cylindrical valve body (5) located in the mount, and openings (3a, 3b, 3c, 3d) for at least two channels at the bottom of the mount (2). The other component comprises an actuating device (9) that is provided with pressing means, by means of which the valve body (5) can be pressed to the bottom of the mount (2).Type: GrantFiled: June 22, 2010Date of Patent: November 18, 2014Assignee: Qiagen GmbHInventors: Rainer Dahlke, Markus Jeziorski, Hans Robert Attig, Jan Claussen, Eva Schaeffer
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Patent number: 8883412Abstract: A size standard, kit includes a size standard, method of defining a size standard and method of analysis using a size standard. The size standard is intended to include size standard elements which have a size greater than and/or less than and/or different from the components of a sample which are to be sized. This means that the same characteristic unit, such as a dye, can be used to label the component and the size standard. A further characteristic unit, from amongst a limited number of such characteristic units is liberated from use only on size standards for use on components. The method is therefore particularly useful in multiplex amplification of STRs.Type: GrantFiled: June 29, 2009Date of Patent: November 11, 2014Assignee: Qiagen GmbHInventors: Valerie Carol Tucker, Pieris Koumi, Andrew John Hopwood, Keith Elliot
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Patent number: 8859749Abstract: The present invention refers to a double-stranded siRNA molecule comprising a sense Strand and an antisense Strand which is essentially complementary to the sense Strand, each of the sense and the antisense Strands comprising at least 17 nucleotides (nt), the siRNA further comprising at least one overhang at the 5? and/or 3? end, wherein the overhang residue or overhang residues are chemically modified and selected independently from each other from the group consisting of: (a) 2?-deoxy modified nucleotides; (b) 2?-methoxy modified nucleotides; (c) two nucleosides linked by a 3? to 5? or 2? to 5? formacetal linkage; (d) nucleotides modified at the 2?-position by a —O—CH2—O—(CH2)2—OH group; and (e) nucleotides comprising in the 3?-position a —CH2—O—(CH2)7—CH3 group.Type: GrantFiled: March 8, 2006Date of Patent: October 14, 2014Assignee: QIAGEN GmbHInventors: Ioanna Andreou, Stefan Pitsch, Evelyne Muller, Luc Reymond