Patents Assigned to QIAGEN GmbH
  • Patent number: 8852863
    Abstract: The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.
    Type: Grant
    Filed: May 4, 2010
    Date of Patent: October 7, 2014
    Assignee: Qiagen GmbH
    Inventors: Thomas Rothmann, Holger Engel, Ralf Himmelreich, Andy Wende, Rainer Dahlke
  • Patent number: 8828709
    Abstract: The invention relates to a method with which proteins from formalin-fixed biological samples can be dissolved and subsequently quantified. The method makes it possible to extract intact full-length proteins from the samples and to conduct a subsequent analysis thereof.
    Type: Grant
    Filed: May 10, 2006
    Date of Patent: September 9, 2014
    Assignee: Qiagen GmbH
    Inventors: Peter Porschewski, Karl-Friedrich Becker
  • Publication number: 20140248606
    Abstract: The present invention refers to a method, a composition and a kit for isolating biomolecules from any biological sample material containing cells, virus(es), microorganism(s) or a combination thereof comprising a cell- or virus-simulating means, wherein said cell- or virus-simulating means comprises at least one type of marker molecule(s), incorporated in at least one type of a layer, capsule, bead, sphere or particle, which is not a biological cell or provided on a substrate covered by a coating.
    Type: Application
    Filed: August 13, 2012
    Publication date: September 4, 2014
    Applicants: QIAGEN HAMBURG GMBH, QIAGEN GMBH
    Inventors: Katharina Beller, Thomas Doedt, Dirk Heckel, Rainer Söller
  • Patent number: 8816063
    Abstract: The present invention relates to a method for purifying a defined amount of nucleic acids from a nucleic acid-containing sample, which has at least the following steps: (a.) contacting the nucleic acid-containing sample with a defined amount of a nucleic acid binding phase with the following features: (i) the nucleic acid binding phase has nucleic acid binding ligands that have at least one protonatable group; (ii) the nucleic acid binding ligands are bound to a carrier; (iii) the nucleic acid binding phase has a surface with a low charge density, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid binding phase used; (b.) binding of the nucleic acids to the nucleic acid binding phase at a pH (binding pH) that is below the pKs value of at least one of the protonatable groups; (c.) elution of the nucleic acids at a pH that is above the binding pH, wherein a defined amount of nucleic acids is obtained.
    Type: Grant
    Filed: May 11, 2010
    Date of Patent: August 26, 2014
    Assignee: Qiagen GmbH
    Inventors: Jan Petzel, Holger Wedler, Roland Fabis
  • Publication number: 20140234834
    Abstract: The invention provides for a method for quantifying one or more nucleic acids of a genome in a sample comprising the steps of (a) amplifying a first nucleic acid to be quantified, (b) determining the amount of said first nucleic acid by comparison of the amount of amplification product from said first nucleic acid with at least one amplification product from a second template nucleic acid, (c) wherein said second template nucleic acid was generated using whole genome amplification and wherein the starting concentration of the second template nucleic acid is known.
    Type: Application
    Filed: February 21, 2012
    Publication date: August 21, 2014
    Applicant: Qiagen GmbH
    Inventors: Francesca Di Pasquale, Holger Engel
  • Publication number: 20140227688
    Abstract: The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using an apoptosis inhibitor, preferably a caspase inhibitor, a hypertonic agent and/or a compound according to formula (1) as defined in the claims.
    Type: Application
    Filed: September 25, 2012
    Publication date: August 14, 2014
    Applicant: Qiagen GmbH
    Inventors: Martin Horlitz, Anabelle Schubert, Markus Sprenger-Haussels
  • Patent number: 8796028
    Abstract: The invention relates to a method for treating a biological sample, comprising the following method steps: i) preparation of a biological sample and ii) bringing the biological sample into contact with a composition, comprising: (1) 1 to 100 wt. % of at least one polyol and (2) 0 to 99 wt. % of at least one additive, wherein the total amount of components (1) and (2) is 100 wt. %. The invention further relates to biological samples obtained by said method, a method for analysis of a treated biological sample, devices for treating a biological sample, use of said devices, various kits and use of a composition.
    Type: Grant
    Filed: December 29, 2006
    Date of Patent: August 5, 2014
    Assignee: Qiagen GmbH
    Inventor: Vera Holländer
  • Publication number: 20140212884
    Abstract: The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an RNA template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction.
    Type: Application
    Filed: August 13, 2012
    Publication date: July 31, 2014
    Applicant: QIAGEN GMBH
    Inventors: Nan Fang, Andreas Missel
  • Patent number: 8785120
    Abstract: The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo-oder heteropolymeres, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carbonxylic acid amides, f) inorganic ammonium sa
    Type: Grant
    Filed: August 24, 2010
    Date of Patent: July 22, 2014
    Assignee: Qiagen GmbH
    Inventors: Christian Korfhage, Friederike Wilmer, Dirk Loffert, Ralf Himmelreich, Claudia Fritz, Kathleen Rieske
  • Publication number: 20140199689
    Abstract: The present invention pertains to a method for isolating nucleic acids from a sample, preferably a blood sample, comprising the following steps: a) obtaining a sample which has been stabilised by the use of at least one cationic detergent, wherein the cationic detergent has formed complexes with the nucleic acids; b) obtaining the complexes optionally together with other sample components from the stabilised sample, wherein said complexes comprise the nucleic acids to be isolated; c) resuspending the complexes and optionally adding one or more additives before, during and/or after resuspension, thereby obtaining a resuspended sample comprising at least: i) the nucleic acid to be isolated; ii) at least one chaotropic agent; and iii) at least one chelating agent; and d) isolating nucleic acids from the resuspended sample.
    Type: Application
    Filed: August 13, 2012
    Publication date: July 17, 2014
    Applicant: QIAGEN GMBH
    Inventor: Thorsten Voss
  • Patent number: 8778842
    Abstract: The present invention provides copolymers that facilitate nucleic acid analysis, compositions that comprise such copolymers, and methods for making or using such copolymers.
    Type: Grant
    Filed: February 6, 2006
    Date of Patent: July 15, 2014
    Assignee: QIAGEN GmbH
    Inventors: Ralf Himmelreich, Roland Fabis, Christoph Erbacher, Sabine Werner, Bernd Springer
  • Patent number: 8771952
    Abstract: The present invention relates to a DNA profiling assay comprising the following steps, providing a sample to be analyzed, providing reagents, enzyme and primeroligonucleotides which are necessary for simultaneous polymerase chain reaction amplification of at least 20 loci, amplifying the loci, detecting the amplification products, wherein the amplification products and the loci to be amplified are characterized by the following features, each locus to be amplified is characterized by at least one deletion-insertion polymorphism known to be present in the population, wherein the two alleles from each locus differ in size by more than 2 nucleotides and less than 100 nucleotides, a first set of at least two amplification products ranging in size from about 20 nucleotides to about 300 nucleotides stemming from at least two different loci carries a first label, a second set of at least two amplification products ranging in size from about 20 nucleotides to about 300 nucleotides stemming from at least two different
    Type: Grant
    Filed: April 24, 2009
    Date of Patent: July 8, 2014
    Assignee: Qiagen GmbH
    Inventors: Manja Bohme, Jorg Gabert, Werner Brabetz
  • Patent number: 8771614
    Abstract: The present invention relates to improved methods for processing fluids and to a fluid processing device (1) for use in a centrifuge comprising: (a) a first holder (14) form-fit to the shape of a first tube (18) for holding said first tube (18) whereby said first tube (18) has a first cross section (A1); and (b) a second holder (22) form-fit to the shape of a second tube (26) for holding said second tube (26) whereby said second tube (26) has a second cross section (A2) that is different from said first cross section (A1). With the fluid processing devices and the methods according to the invention, it is possible to simplify the centrifugal processing steps for a given fluid processing sequence and to automate them.
    Type: Grant
    Filed: September 26, 2006
    Date of Patent: July 8, 2014
    Assignee: Qiagen GmbH
    Inventors: Andreas Schaefer, Thomas Voit, Markus Zbinden, Andreas Schmiede
  • Publication number: 20140158619
    Abstract: The present invention relates to a filtering device comprising clay minerals for the filtration of aqueous solutions used in biochemical and molecular biological applications. In particular, the present invention relates to the removal of undesired proteins from aqueous solutions by filtration through a filter comprising clay minerals.
    Type: Application
    Filed: July 20, 2012
    Publication date: June 12, 2014
    Applicant: QIAGEN GMBH
    Inventor: Gabriele Christoffel
  • Publication number: 20140154748
    Abstract: Novel thermostable chimeric nucleic acid polymerases and methods for their generation and use are disclosed. It is shown that these chimeric nucleic acid polymerases, such as DNA polymerases, can be constructed using enzymatically active domains, isolated from different proteins or chemically synthesized. It is demonstrated that chimeric nucleic acid polymerases of the present invention possess the chemical and physical properties of their component domains (e.g., exonuclease activity, thermostability) and that the chimeric polymerases are thermostable.
    Type: Application
    Filed: December 17, 2013
    Publication date: June 5, 2014
    Applicant: QIAGEN GMBH
    Inventors: Dirk Löffert, Andreas Missel, Jie Kang
  • Publication number: 20140154693
    Abstract: The present invention provides a special reagent for the pretreatment of sample materials. The pretreatment with the reagent according to the invention enables the isolation of nucleic acids by a uniform method from very diverse sample materials, in particular different bioprocess samples, even if the nucleic acids are only present in small amounts in the sample materials. The reagent used for the pretreatment comprises, as key component, at least one compound comprising an amino group. The invention further relates to methods of purification of nucleic acids, in which the sample material is pretreated correspondingly, and suitable kits.
    Type: Application
    Filed: July 4, 2012
    Publication date: June 5, 2014
    Applicant: QIAGEN GmbH
    Inventor: Peter Porschewski
  • Publication number: 20140147843
    Abstract: The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.
    Type: Application
    Filed: February 20, 2012
    Publication date: May 29, 2014
    Applicants: QIAGEN MANCHESTER LIMITED, QIAGEN GMBH
    Inventors: Francesca Di Pasquale, Holger Engel, Sascha Strauss, Nicola Jo Thel Well
  • Publication number: 20140140804
    Abstract: Magnetic rack system comprising a holder having trough-holes for receiving tubes, a base having a plurality of receptacles with at least one magnet, wherein trough-holes in the holder are arranged relative to the base such that each tube can be positioned in a respective receptacle in a pre-determined position relative to the magnet, and an adapter, wherein the adapter is designed to allow transmission of motion to the tubes (2) positioned in the holder (1).
    Type: Application
    Filed: October 18, 2013
    Publication date: May 22, 2014
    Applicant: QIAGEN GMBH
    Inventors: Maximilian FOCKE, Lothar BREITKOPF, Karen KOWALEWSKI, Thorsten SINGER
  • Patent number: 8729252
    Abstract: A method for isolating polynucleotides, such as DNA, RNA and hybrids thereof from an aqueous solution containing polynucleotides by reversibly binding the polynucleotides to silica-coated magnetic particles in the presence of a salt and non-ionic hydratable additive is disclosed. The salt and non-ionic hydratable additive concentrations are adjusted to levels that result in adhesion of the nucleic acid to the particles without degradation or precipitation of the nucleic acid.
    Type: Grant
    Filed: August 16, 2004
    Date of Patent: May 20, 2014
    Assignee: Qiagen GmbH
    Inventors: Ralf Himmelreich, Sabine Werner
  • Patent number: 8728786
    Abstract: Disclosed are methods for the enhancement of the reactivation of thermostable reversibly inactivated enzymes comprising reactivating at least one thermostable reversibly inactivated enzyme in the presence of one or more nitrogen containing compounds.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: May 20, 2014
    Assignee: QIAGEN GmbH
    Inventors: Dirk Löffert, Ralf Peist, Patrick Baumhof