Abstract: The present invention provides improved methods that allow accurate monitoring and/or control of temperature changes in a microfluidic environment. An advantage of the present invention is that the temperature can be monitored and/or controlled at any location within a microfluidic device, especially where a preparation step, an amplification step and/or a detection step is performed. The invention further provides improved microfluidic devices for practicing the methods disclosed and claimed herein.
Type:
Grant
Filed:
March 9, 2018
Date of Patent:
October 26, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Hamed Shadpour, Sean Ford, Jorge Alberto Garces, Darren S. Gray
Abstract: The invention, depending on aspect and embodiment, relates to capture probe controls, and capture and signal probe configurations and combinations of configurations that can facilitate accurate and efficient multiplex analyte detection, especially in electrochemical detection schemes.
Type:
Grant
Filed:
April 28, 2020
Date of Patent:
October 26, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Michael Leonard Wood, Aviva Ruth Jacobs
Abstract: The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70° C. for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method.
Abstract: Accurate measurements of the presence or absence of a target cell in a sample are provided. For example, the sample can be mixed with a plurality of transduction particles capable of binding to the target cells, the transduction particles being engineered to include a nucleic acid molecule formulated to cause the target cells to produce a plurality of detectable reporter molecules once the particles bind to and deliver the nucleic acid molecules into the one or more target cells. A set of signal data points are received that are associated with a quantity of reporter molecules and the signal data points are analyzed to accurately detect target cells in the sample. Systems and methods are disclosed.
Type:
Grant
Filed:
December 19, 2017
Date of Patent:
October 19, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Corey Hoeppner, Ronald T Kurnik, Xianxian Liu, Soni Shukla
Abstract: The subject disclosure provides systems, computer-implemented methods, and clinical workflows for meso-dissection of biological specimens and tissue slides by incorporating annotation and inter-marker registration modules within digital pathology imaging and meso-dissection (or milling) systems. Images of a reference slide a milling slide may be acquired using the same imaging system, with the annotations on the image associated with the milling slide being based on the inter-marker registration. Each image along with its respective annotations and meta-data may be associated with a project or a case, and stored in an image management system. A same-marker registration may be used to map annotations from the annotated image of the milling slide to a live image of the milling slide. The milling slide may be milled based on the annotations, with milled tissue output into a contained that is labeled in association with the labeled input slides.
Type:
Grant
Filed:
July 25, 2017
Date of Patent:
September 21, 2021
Assignee:
ROCHE MOLECULAR SYSTEMS, INC.
Inventors:
Michael Barnes, Srinivas Chukka, Mohammad Qadri
Abstract: Package assemblies for storing diagnostic cartridges or storing wet/dry reagents are described herein. In some embodiments, an apparatus includes a tray member defining a first volume and a second volume, and a cover member coupled to the tray member covering the first volume and the second volume. The tray member includes a central portion that separates the first volume from the second volume. The first volume is configured to receive a desiccant package and a sample container containing a first reagent. The first reagent has a solid form. The second volume is configured to receive a reagent module containing a second reagent. The second reagent has a liquid form. The cover member and the central portion of the tray member are configured to isolate the first volume from the second volume.
Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
Type:
Grant
Filed:
November 26, 2018
Date of Patent:
August 3, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Alexander Nierth, Hannes Kuchelmeister, Concordio Anacleto, Amar Gupta, Dieter Heindl, Igor Kozlov, Christian Wellner, Stephen Gordon Will
Abstract: A device is described including a lid, a collection portion support, and a collection portion with a surface for receiving a biological sample, the lid includes a window and a window holder, the surface defines a first plane, the collection portion includes a first, a second, and a third position markers, each position marker indicates a center point marker, the center points define a second plane that has a line of intersection with the first plane, the device has an interior volume defined partially by the collection portion support, the collection portion, the window, and the window holder, the window allows visual inspection of the position markers, the collection portion support has a fluid inlet and a fluid outlet with a fluid path from the fluid inlet to the fluid outlet via the interior volume.
Type:
Grant
Filed:
March 16, 2018
Date of Patent:
July 20, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Andreas Drechsler, Edwin Oosterbroek, Emad Sarofim, Heiko Schwertner
Abstract: According to the present invention, a system (1; 1?) for processing at least one substrate (2) containing a dried fluid sample (21) is provided, the system (1; 1?) comprising a support (12) configured to position the substrate (2), a laser device (3) for directing a laser beam (31) to the substrate (2), configured to cut at least one area of the substrate (2) containing the dried fluid sample (21) by means of the laser beam (31), a container holder (4; 4?) configured to hold and position a container (5) for receiving the cut area, the container holder (4; 4?) being arranged below the substrate (2), and an extraction subsystem (6) for extracting fume and/or dust generated when laser cutting the substrate (2), wherein the extraction subsystem (6) consists of at least two extraction components (61, 62) sandwiching the substrate (2) there between. Furthermore, a method for automated processing at least one substrate (2) containing a dried fluid (21) sample by means of such a system (1; 1?) is provided.
Type:
Grant
Filed:
May 17, 2018
Date of Patent:
July 6, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Claudio Cherubini, Andreas Drechsler, Reto Huesser, Emad Sarofim
Abstract: The present invention describes a base module (100) and a tray insert (200, 300, 400) of a multipurpose tray (100) for an automated processing system, such as an analytical, pre-analytical or post-analytical processing system, as well as to a multipurpose tray comprising such base module and such tray insert, wherein the tray insert can be particularly used for holding a plurality of reagent or sample tubes to be processed in the automated processing system. The present invention further relates to a method of simplified loading/unloading of such a multipurpose tray into/from the automated processing system.
Type:
Application
Filed:
October 18, 2018
Publication date:
June 17, 2021
Applicant:
Roche Molecular Systems, Inc.
Inventors:
Lawrence Chiu, Ion Iordanoaia, Reiner Hitt, Vijay Namasivayam, Eduard Nawrocki, Michael Neugebauer, Tim Moulton, Sam Palmer, Gerd Schmieta
Abstract: The present invention describes a laboratory system for automatically processing at least one sample container containing a biological sample, the laboratory system comprising a housing; laboratory instrument units for executing processing steps on the biological sample; an input station configured to receive the sample container; a transport means for transport of the sample container from the input station to the laboratory instrument units, and further to an output station; a control unit for determining whether the sample and/or the container is in a condition to be processed by the laboratory instrument units; an input-output station providing an interface between the inside and the outside of the housing; and a workbench on the outside of the housing in front of the input-output station for an operator to be in the position to manipulate the sample and/or the container.
Type:
Application
Filed:
October 18, 2018
Publication date:
June 17, 2021
Applicant:
Roche Molecular Systems, Inc.
Inventors:
Andreas Gisler, Mike Mohns, Vijay Namasivayam, Maren Christin Wolff, Devorah Klein, Tim Moulton, Sam Palmer, Eric Saperstein
Abstract: Devices, systems, and associated methods for selectively extracting a material from a sample are provided. In one aspect, for example, a method for selectively extracting biological material from a biological sample can include identifying a region of biological material to be extracted from a biological sample, applying an extraction tool to the region of biological material to disrupt biological material from the biological sample, and dispensing a liquid at the region of biological material. The method can also include aspirating the liquid and the disrupted biological material from the biological sample.
Type:
Application
Filed:
November 12, 2020
Publication date:
June 17, 2021
Applicant:
Roche Molecular Systems, Inc
Inventors:
Nils B. Adey, Katherine B. Geiersbach, Mark Herrmann, Robert J. Parry
Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
Type:
Grant
Filed:
September 15, 2017
Date of Patent:
June 15, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
Abstract: Methods for the rapid detection of the presence or absence of Mycobacterium tuberculosis (MTB) and other members of the MTB-complex in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the esxJ gene, along with kits are provided that are designed for the detection of MTB.
Abstract: Systems, methods, and apparatuses are provided for detecting nucleic acid sequence variants. Other embodiments are directed to systems and computer readable media associated with methods described herein.
Abstract: The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
Type:
Grant
Filed:
September 15, 2017
Date of Patent:
June 8, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
Abstract: The present invention provides inactivated microbes, methods of preparing and using the same, as well as compositions and kits containing the same. The inactivated microbes are useful in the formulation of internal control reagents for use in recombinant nucleic acid techniques, especially nucleic acid amplification, e.g., by the polymerase chain reaction (PCR).
Inventors:
Qianwei Xie, Marc Mailman, Vishakha Sharma, Veronica Nemmer, Elizabeth Little, Wojciech Kaczmarek, Maciej Chmielarski, Tomasz Sciurka, Szymon Dolata, Maciej Laskowski
Inventors:
Qianwei Xie, Marc Mailman, Vishakha Sharma, Veronica Nemmer, Elizabeth Little, Wojciech Kaczmarek, Maciej Chmielarski, Tomasz Sciurka, Szymon Dolata, Maciej Laskowski