Abstract: There is provided a method by which multiple types of substances desired to be transferred into cells can be continuously transferred into multiple types of cells by a convenient procedure, a cell in which the substance desired to be transferred into cells has been taken up by this method, and an apparatus for transferring a substance into cells by this method. The foregoing objects can be achieved by electrospraying cells with a liquid free from the substance to be transferred into cells while the cells are kept in contact with the substance to be transferred into cells, or first electrospraying cells with a liquid free from the substance to be transferred into cells and then bringing the cells into contact with the substance to be transferred into cells.

Abstract: According to the present invention, n (n=S is a positive integer) pieces of interrupting grids (22-1, 22-2, 22-3, - - - , 22-(n-1) and 22-n) are provided, each of the interrupting grids encompasses P pieces of narrow cut-off canals arranged in parallel. The both side of each of the narrow cut-off canals are concaved so that the narrow cut-off canal has a waisted-mortar shape. The waisted-mortar shape is delineated by m pieces of elliptical holes (Q1, Q2, Q3, - - - , Qm-1 and Qm (m=P?1 is a positive integer)) arranged adjacently in parallel and semi-elliptical holes (cut ID portions) provided on both sides the alignment of elliptical holes. Then, n pieces of the interrupting grids 22-1, 22-2, 22-3, - - - , 22-(n-1) and 22-n are arranged in series through jointing zones (heat-radiation zones) (21-1, 21-2, 21-3, - - - , 21-(n-) and 21-n) having a length of 2.5 millimeters or less measured in the series direction.

Abstract: The image processing apparatus of the present invention comprises signed distance calculation unit calculating signed distance fields from each input 3D shape data, signed distance integration unit integrating all the signed distances calculated by signed distance calculation unit for each shape data, classification unit classifying each voxel to be outside of the observed object, near the surface of the object or the other, estimation unit estimating whether voxel is inside or outside of the object, and determination unit determining voxel to be inside, outside or near-surface of the object based on the calculation results of the previous units.

Abstract: [Problems] To provide a series electric double-layer capacitor device having a simple structure eliminating nonuniform voltages induced among electric double-layer capacitors (C1 to C6). [Means for Solving Problems] A series electric double-layer capacitor device comprises 2n capacitors (C1 to C6), 2n diodes (D1 to D6), a transformer (T1), and an inverter (31). Secondary wirings (N1 to N3) of the transformer supply the induced voltages to a capacitor module (30) composed of sets of two capacitors and two diodes. The capacitors are charged when the diodes included in the capacitor module are conductive. The series electric double-layer capacitor device further comprises voltage adjusting means (32) for changing the DC voltage level between terminals (1, 2), and the inverter converts the voltage-changed DC voltage into an AC voltage. A voltage for canceling the forward voltage drops of the diodes is added to the induced voltages of the secondary wirings by the operation of the voltage adjusting means.

Abstract: The present invention provides a novel method of significantly promoting the ratio of homologous recombination in desirable cells. In order to enhance the ratio of homologous recombination in desirable cells such as eukaryotic cells, mutation is introduced into genes encoding factors necessary for non-homologous recombination, such as Ku70 or Ku80, or the above genes are disrupted, so as to cause the loss of the functions thereof. At the time, foreign DNA is introduced into the cells via the electroshock method or the like, so as to carry out homologous recombination, thereby promoting the frequency of homologous recombination in the cells.

Abstract: [Problems] To provide a non-contact power feeder that is high efficient and high power factor and has no load dependence. [Means for Solving Problems] A series capacitor Cs1 is connected to a primary winding 1 driven by an AC power supply 3 and a parallel capacitor Cp2 is connected to a secondary winding 2. The capacitance Cp is set to Cp?1/{2?f0×(x0+x2)} and the capacitance Cs converted to the primary side is set to Cs?(x0+x2)/{2?f0×(x0×x1+x1×x2+x2×x0)}, where f0 is the frequency of the power supply, x1 is a primary leakage reactance of the primary winding 1, x2 is a secondary leakage reactance of the secondary winding 2 converted to the primary side and x0 is an excitation reactance converted to the primary side. By setting Cp and Cs to the above values, the transformer of the non-contact power feeder is substantially equivalent to an ideal transformer. If it is driven by a voltage type converter, the output voltage (=load voltage) becomes substantially constant voltage regardless of the load.

Abstract: According to the present invention, an analysis system constructed as a rheology model of a foundation-ground system that is capable of expressing a frequency dependent dynamic spring by using elements with non-frequency-dependent coefficients may be provided. The analysis system according to the present invention is a model for reproducing dynamical characteristics of a system including the foundation and the ground. The analysis system includes an elastic element, a damper element for damping vibration, and a reaction force generation element that generates reaction force proportional to relative acceleration of both ends thereof. The analysis system is constructed as a base system in which the elastic element, the damper element and the reaction force generation element are connected in parallel.

Abstract: There is provided a method by which multiple types of substances desired to be transferred into cells can be continuously transferred into multiple types of cells by a convenient procedure, a cell in which the substance desired to be transferred into cells has been taken up by this method, and an apparatus for transferring a substance into cells by this method. The foregoing objects can be achieved by electrospraying cells with a liquid free from the substance to be transferred into cells while the cells are kept in contact with the substance to be transferred into cells, or first electrospraying cells with a liquid free from the substance to be transferred into cells and then bringing the cells into contact with the substance to be transferred into cells.

Abstract: [Problem]To provide an actuator drive device that eliminates the need for high voltage amplifiers. [Means for Solving Problem]The electrostatic actuator drive device comprises a variable capacitor 12 connected in series with an electrostatic actuator 10, and a constant voltage source 11 for applying a constant voltage between the both ends of the serial connected electrostatic actuator 10 and the variable capacitor 12. The capacitance of the variable capacitor 12 is varied under control of a controller 14 for adjusting the voltage to be applied to the electrostatic actuator 10. The constant voltage source 11 may be any forms to apply a constant high voltage. The electrostatic actuator 10 is equivalently a capacitor. In this device, the capacitance of one of series connected two capacitors is varied for adjusting the voltage to be applied to the other capacitor, or the electrostatic actuator 10.

Abstract: A microphone-capsule includes a vibrator implemented by a conductor having a flat vibration surface, a dielectric-polarization plate that is defined by a flat first principal surface facing the vibration surface of the vibrating plate and a second principal surface facing parallel to the first principal surface and in which polarization directions are aligned, a back electrode joined to the second principal surface of the dielectric-polarization plate, and an induced-charge measuring unit configured to measure the charges induced between the vibrating plate and the back electrode, in association with the displacement of the vibration surface. The induced-charge measuring unit includes an amplifier connected to the back electrode and an output circuit connected to the amplifier.

Abstract: It is an object of the preset invention to provide an activity measurement molecule necessary for measuring biological reactions or changes in in vivo conditions, and a method for measuring activity using the above activity measurement molecule. It is intended to provide an activity measurement molecule used for simultaneously analyzing multiple biological reactions and/or changes in in vivo conditions, which is characterized in that one or more fluorescent molecule-labeled and/or -unlabeled biomolecules used as targets of the biological reactions or changes in in vivo conditions bind onto a quantum dot, and a method for simultaneously analyzing multiple biological reactions and/or changes in in vivo conditions using the above activity measurement molecule.

Abstract: The present invention provides a novel method of significantly promoting the ratio of homologous recombination in desirable cells. In order to enhance the ratio of homologous recombination in desirable cells such as eukaryotic cells, mutation is introduced into genes encoding factors necessary for non-homologous recombination, such as KU70 or KU80, or the above genes are disrupted, so as to cause the loss of the functions thereof. At the time, foreign DNA is introduced into the cells via the electroshock method or the like, so as to carry out homologous recombination, thereby promoting the frequency of homologous recombination in the cells.

Abstract: [Problems] To provide a surface acoustic wave excitation device for efficiently exciting the surface acoustic wave on the surface of a material such as glass having free size and shape for making mechanical vibration available. [Means to Solve the Problems] The surface acoustic wave excitation device according to the present invention comprises a non-piezoelectric member 30 such as glass, a piezoelectric member 20, interdigital transducers 11 interposed between the non-piezoelectric member 30 and the piezoelectric member 20, and pre-pressurizing means 42 for pressing the piezoelectric member 20 onto the non-piezoelectric member 30 by way of the interdigital transducers. Alternating voltage is applied to the interdigital transducers 11 for exciting the surface acoustic wave on the non-piezoelectric member 30.

Abstract: [Problems] To provide a surface acoustic wave excitation device for efficiently exciting the surface acoustic wave on the surface of a material such as glass having free size and shape for making mechanical vibration available. [Means to Solve the Problems] The surface acoustic wave excitation device according to the present invention comprises a non-piezoelectric member 30 such as glass, a piezoelectric member 20, interdigital transducers 11 interposed between the non-piezoelectric member 30 and the piezoelectric member 20, and pre-pressurizing means 42 for pressing the piezoelectric member 20 onto the non-piezoelectric member 30 by way of the interdigital transducers. Alternating voltage is applied to the interdigital transducers 11 for exciting the surface acoustic wave on the non-piezoelectric member 30.

Abstract: A deformation measuring method using electronic speckle pattern interferometry comprises the steps of subtracting an average intensity from the intensity in a time domain at each point of a speckle pattern image so as to compute the cosine component of intensity; subjecting the cosine component to Hilbert transform in a temporal domain so as to compute the sine component of intensity; determining the arctangent of the ratio between thus computed sine and cosine components so as to determine an object phase; carrying out an unwrapping operation; and outputting three-dimensional deformation distribution data in a displayable mode.

Abstract: A deformation measuring method using electronic speckle pattern interferometry comprises the steps of subtracting an average intensity from the intensity in a time domain at each point of a speckle pattern image so as to compute the cosine component of intensity; subjecting the cosine component to Hilbert transform in a temporal domain so as to compute the sine component of intensity; determining the arctangent of the ratio between thus computed sine and cosine components so as to determine an object phase; carrying out an unwrapping operation; and outputting three-dimensional deformation distribution data in a displayable mode.

Abstract: A deformation measuring method using electronic speckle pattern interferometry comprises the steps of subtracting an average intensity from the intensity in a time domain at each point of a speckle pattern image so as to compute the cosine component of intensity; subjecting the cosine component to Hilbert transform in a temporal domain so as to compute the sine component of intensity; determining the arctangent of the ratio between thus computed sine and cosine components so as to determine an object phase; carrying out an unwrapping operation; and outputting three-dimensional deformation distribution data in a displayable mode.

Abstract: An imaging spectral device for use in the spectrum image analysis for performing the spectroscopic analysis of the respective points in two dimensional field is disclosed. The device comprises an imaging spectral device composing a white-light source to illuminate an object to be measured, a tunable filter located in the optical path between the object and the white-light source, a driving mechanism for wavelength scanning of the tunable filter, and a control unit in which scanning rate of transmitting wavelength of the tunable filter is controlled by the above-mentioned driving mechanism in such a manner that the spectral transmittance of the tunable filter integrated within the exposure time becomes desired spectral distribution of the object to be measured.

Abstract: The stacked microstrip antenna has a ground plane, a first dielectrical layer, a first radiating element, a second dielectric layer, a second radiating element and a short-circuiting conductor for short-circuiting between the first and second radiating elements and the ground plane. The stacked microstrip antenna attains double-channel duplex characteristics with utilizing the coupling between the first radiating element and the second radiating element, when a power is fed to the antenna. Further, the widthwise dimension of the short-circuiting conductor is controlled, whereby the antenna leads to the miniaturization of the radiating elements, namely, the miniaturization of an antenna proper, and it is permitted to be tuned to two desired frequencies with ease.