Abstract: A clamp for closing a flexible tube includes an anvil and a hammer. The anvil includes a passage and a slot defined therein. The passage is configured to receive a flexible tube therethrough. The hammer is slidably received within the slot of the anvil. The hammer is securable in a first position in which the passage of the anvil is substantially unoccluded and a second position in which the passage of the anvil is occluded. The hammer and the anvil are configured to prevent fluid from flowing through the flexible tube received in the passage when the hammer is in the closed position. The hammer is substantially within the anvil in the closed position.

Abstract: The disclosure provides example embodiments for automatically or semi-automatically classifying cells in microscopic images of biological samples. These embodiments include methods for selecting training sets for the development of classifier models. The disclosed selection embodiments can allow for the re-training of classifier models using training examples that have been subjected to the same or similar incubation conditions as target samples. These selection embodiments can reduce the amount of human effort required to specify the training examples. The disclosed embodiments also include the classification of individual cells based on metrics determined for the cells using phase contrast imagery and defocused brightfield imagery. These metrics can include size, shape, texture, and intensity-based metrics. These metrics are determined based on segmentation of the underlying imagery.

Abstract: Systems and a method for transferring chemical, pharmaceutical, and/or biological material into or out of a container are provided. One system comprises a disposable container having at least one port for accessing the interior of the container, the port comprising at least one connecting protrusion extending parallel to the container. The system further comprises a transfer interface connectable to the port. The transfer interface comprises a plate, and at least one connecting flange extending from the plate, the connecting flange to be arranged under the respective connecting protrusion to connect the transfer interface to the port, such that when the transfer interface is connected to the port the plate is parallel to a surface of the container.

Abstract: The present invention is directed to the development of continuous processing technology for the purification of biopharmaceuticals and biological products, such as monoclonal antibodies, protein therapeutics, and vaccines. Methods for continuous processing of a biological product in a feed stream toward formulation of a purified bulk product are described.

Abstract: Systems and methods for characterizing a plurality of particles suspended in a solution are described. In some embodiments, an ultrasonic interrogation signal may be emitted into a solution including a plurality of particles suspended in the solution. A resulting ultrasonic spectrum may be sensed and provided to a trained statistical model of the solution. The trained statistical model may then determine one or more properties of the plurality of particles.

Abstract: A bioreactor for culturing of microorganisms or cells comprising: a casing defining an interior space of the bioreactor, wherein said interior space is configured to receive and hold a culture medium with microbial or cellular liquid cultures; and at least one coupling element provided on the casing for coupling with a foam mitigation device for mitigation of foam built-up in the interior space of the bioreactor during use of the bioreactor for culturing of microorganisms or cells; wherein the coupling element is configured to allow ultrasonic waves generated by the foam mitigation device act upon foam built-up in the interior space of the bioreactor.

Abstract: The present invention relates to an adsorption medium including polymeric space elements which have been bonded to the surface of the chromatography matrix, and polymer chains containing chromatographically active centers, wherein the polymer chains have been bonded to the polymeric spacer elements, as well as to a method for the production thereof, and to the use of the adsorption medium for the purification of biomolecules.

Abstract: The invention relates to a filtration device and to a method for characterizing a filter medium or a medium to be filtered. The filtration device comprises a plurality of filtration units. Each filtration unit comprises a first container, which is designed to receive a medium to be filtered, a second container, which is designed to receive a filtrate, at least one connecting line, which connects the first container to the second container, and a filter medium between the first container and the second container in order to filter the medium to be filtered. The first container is designed for the application of positive pressure thereto, and the second container is designed to receive a measurement instrument at ambient pressure.

Abstract: The invention relates to an apparatus 10 for storing and/or processing at least one medium 11, in particular a bioprocessing device, comprising at least one disposable container 1, which is designed to accommodate at least some of the at least one medium 11, and at least one overpressure protection means 5, which is fluid-connected to the at least one disposable container 1. The at least one overpressure protection means is designed, when triggered, to conduct at least some of the at least one medium 11 into an apparatus region 3a? in front of the overpressure protection means 5 in relation to a flow direction F, in particular into the at least one disposable container 1 and/or into at least one further, second container 1a.

Abstract: A device and a method for repeatedly modifying the composition of a fluid. The device includes a first module (19) modifying the composition of the fluid, a second module (21) modifying the composition of the fluid and a dwell module (20) with an inlet (8) and an outlet (10). The first module is connected in a fluid-conducting manner to the dwell module inlet and the dwell module outlet is connected in a fluid-conducting manner to the second module. Either the first or the second module is a filter unit, or the first module is a first filter unit and the second module is a second filter unit. The filter unit(s) include(s) at least one first filter medium (4, 14) delimiting a supply channel (2, 12) and a retentate channel (1, 11) and at least one second filter medium (5, 15) delimiting the retentate channel and a permeate channel (3, 13).

Abstract: The present invention relates to a diatomaceous earth composition with a low endotoxin content, comprising an agglomerated mixture of calcined diatomaceous earth particles and water, wherein the mass ratio of calcined diatomaceous earth particles and water is in the range of 1:1.0 to 1:2.0, and wherein the endotoxin content of the diatomaceous earth composition is equal to or less than 0.5 EU/mL. In a further aspect, the present invention relates to a method for producing the diatomaceous earth composition with a low endotoxin content. In another aspect, the present invention relates to the use of the diatomaceous earth composition with a low endotoxin content as a precoat agent for the precoat filtration in biopharmaceutical applications.

Abstract: The present invention relates to a multiparameter lateral flow strip (1), comprising a microporous membrane layer (2) supported on a liquid-impermeable support layer (3), for lateral flow of a liquid through the microporous membrane layer (2), wherein the microporous membrane layer (2) has two or more flow lanes (4) in the direction of lateral flow, wherein said two or more flow lanes (4) are separated by hydrophobic separation channels (5), and wherein each of said two or more flow lanes (4) comprises a detection spot (6) including a binding agent, wherein said two or more flow lanes (4) are isomorphic lanes. Furthermore, the present invention relates to a multiparameter lateral flow immunoassay device comprising said multiparameter lateral flow membrane (1), the use of said multiparameter configured lateral flow membrane (1) in an immunological test, as well as to a method for the manufacture of said multiparameter configured lateral flow membrane (1).

Abstract: A method of molding an article includes positioning a dissolvable insert within a mold, filling the mold with material to form an article within the mold and about the dissolvable insert, and at least partially dissolving the dissolvable insert from within the article. The dissolvable insert forms internal features of the article and includes a porous core.

Abstract: A method for mounting at least one component on an installation includes attaching a carrier directly or indirectly to the installation, where the installation has at least one frame with at least two arms, at least one base plate, and at least one deformable element. The method further includes displacing the component toward the deformable element such that the deformable element is displaced from a receiving state (AUZ), in which the deformable element is ready to receive the component of the installation, toward a locked state (ARZ), as a result of which the deformable element surrounds and mounts the component of the installation at least in part.

Abstract: A method for providing an integrity test of a double filter capsule can include providing the double filter capsule in such a way that in a filtration process the medium to be filtered flows in its flow direction from an upstream chamber in the housing through a first filter into an intermediate chamber between the first filter and a second filter and then through the second filter to an outlet. The housing can have an upstream-chamber access point for feeding a test fluid into the upstream chamber and an intermediate-chamber access point for feeding the test fluid into the intermediate chamber. Additional steps can include providing a connecting line between the upstream-chamber access point and the intermediate-chamber access point and determining the state of integrity of the double filter capsule on the basis of at least two test phases.

Abstract: A valve setup for SMB chromatography includes a cassette block, a control block, a swiveling door, and a flexible pressurizing tube. The cassette block is configured to control each of a plurality of valves when an operation surface is in close contact with a control surface of the cassette block. The swiveling door is hinged to the valve control block such that an open state of the door arranges the cassette block such that the control surface is at the operation surface of the control block, and a closed position holds the cassette block with an inner surface of the swiveling door abutting a rear surface of the cassette block opposite the control surface. The flexible pressurizing tube is arranged at the inner surface of the swiveling door so the pressurizing tube presses against the rear surface of the cassette block when the pressure in the tube is increased.

Abstract: A tilting mechanism including a base, a tilting deck, four hinges, and a lifting member. Each hinge is positioned adjacent a corner of the base and the tilting deck. Each hinge includes a globe and a receiver with the globe or the receiver secured to the base frame or the deck frame and the other secured to the other of the base or the tilting deck. Each hinge includes a locking member that selectively secures a globe within a respective receiver. The lifting member is secured at a center of the base frame and a center of the deck frame. The lifting member is configured to urge the tilting deck away from the base such that selective engagement allows for tilting of the tilting deck relative to the base in at least four directions.

Abstract: A computer-implemented method can configure and/or setup and/or control a downstream process for processing a biomass, including receiving at least one measurement value of at least one feature of an upstream process by which the biomass to be processed in the downstream process has been obtained and/or of at least one feature of a downstream process stage or operation, determining at least one downstream process parameter based on the received at least one measurement value, arid configuring and/or setup and/or controlling at least one downstream process stage or process operation based on the determined at least one downstream process parameter.

Abstract: A chromatography system is provided. The chromatography system is configured to process a feed fluid containing a plurality of components, wherein at least one component of the plurality of components of the feed fluid is a target component. The chromatography system comprises: a flow path comprising a plurality of fluid control components configured to control a fluid flow; a stationary phase, wherein the stationary phase is at least one membrane adsorber connected to the flow path and the stationary phase is configured to isolate the target component. The flow path is configured such that harvesting of the target component is optimized.

Abstract: The invention relates to a protective housing for a biopharmaceutical liquid bag having a lower shell with a bottom and side walls, and an upper shell. The upper shell includes a housing for receiving the bag, and can be inserted into the lower shell, in such a way that the opening of the housing is adjacent to and facing the bottom of the lower shell, and in such a way that the upper shell is laterally held in place between the side walls of the lower shell.