Abstract: Provided herein are methods, compositions and kits to extract and relatively enrich by physical separation or amplification short base pair nucleic acid in the presence of a high background of genomic material (e.g., host or maternal nucleic acids).
Type:
Application
Filed:
February 29, 2024
Publication date:
November 21, 2024
Applicant:
SEQUENOM, INC.
Inventors:
Carolyn R. HOYAL-WRIGHTSON, Andreas BRAUN, Karsten E. SCHMIDT
Abstract: Provided herein are methods of normalizing nucleic acid libraries. The method uses nucleic acid probes with nucleic acid sequences that are complementary to one or more of these adaptor sequences are added to the nucleic acids libraries. The probes can hybridize to the adaptor sequences in the single stranded nucleic acid molecules derived from the libraries to form hybridization complexes. The probes are conjugated to a first binding member, which can interact with a second binding member that is conjugated to solid supports. The solid supports can then be collected and the single stranded nucleic acid molecules can be recovered in a volume of elution buffer to reach a desired concentration. As compared to standard methods, the methods are more efficient and cost-effective.
Abstract: Provided herein are methods and compositions to extract and enrich by, physical separation or amplification, relatively short nucleic acids from a nucleic acid composition containing a high background of longer nucleic acids (e.g., host or maternal nucleic acids; genomic nucleic acid and the like).
Type:
Grant
Filed:
November 3, 2020
Date of Patent:
September 3, 2024
Assignee:
SEQUENOM, INC.
Inventors:
Michele Elizabeth Wisniewski, William Hang Kwong, Firouz Mohsenian, Jian-Hua Ding
Abstract: Technology provided herein relates in part to methods, processes, machines and apparatuses for non-invasive assessment of genetic alterations. In particular, a method is provided for that includes obtaining nucleic acid fragments from a sample from a test subject; sequencing the sequence constructs to obtain sequence reads; demultiplexing the sequence reads to a first and a second subset of sequences reads; generating a first set of consensus reads that correspond to the first nucleic acid fragment based on SMBs associated with the first subset of sequences reads; generating a second set of consensus reads that correspond to the second nucleic acid fragment based on SMBs associated with the second subset of sequences reads; and determining a presence of one or more genetic alterations for the test subject based on the two sets of consensus reads.
Type:
Application
Filed:
February 1, 2024
Publication date:
August 29, 2024
Applicant:
Sequenom, Inc.
Inventors:
Mostafa Azab, Michael Sykes, Youting Sun, Amin Mazloom, Taylor Jensen, Mathias Ehrich, Christopher Ellison
Abstract: Provided herein are methods of normalizing nucleic acid libraries. The method uses nucleic acid probes with nucleic acid sequences that are complementary to one or more of these adaptor sequences are added to the nucleic acids libraries. The probes can hybridize to the adaptor sequences in the single stranded nucleic acid molecules derived from the libraries to form hybridization complexes. The probes are conjugated to a first binding member, which can interact with a second binding member that is conjugated to solid supports. The solid supports can then be collected and the single stranded nucleic acid molecules can be recovered in a volume of elution buffer to reach a desired concentration. As compared to standard methods, the methods are more efficient and cost-effective.
Abstract: The present invention relates to systems and methods for non-invasive assessment of genetic variation. In particular, aspects are directed to a computer-implemented method that includes ligating nucleic acid molecules with adapters to generate sequence constructs, sequencing the sequence constructs to obtain sequence reads, generating an alignment computer file including on-target sequence reads and associated genomic positioning data, generating a probe coverage data file for the sample using the on-target sequence reads and the associated genomic positioning data, generating segments and associated probe coverage quantification data for each segment using a segmentation model and the probe coverage data file, identifying genes overlapping with the segments, generating filtered segments based on the identified genes, and determining a presence or absence of a genetic variation in the sample based on the filtered segments.
Type:
Application
Filed:
February 1, 2024
Publication date:
July 11, 2024
Applicant:
SEQUENOM, INC.
Inventors:
Yijin Wu, Amin Mazloom, Yang Zhong, Mostafa Azab
Abstract: Provided herein are methods, processes and apparatuses for non-invasive assessment of genetic variations that make use of nucleic acid fragment length information.
Type:
Application
Filed:
September 1, 2023
Publication date:
May 16, 2024
Applicant:
SEQUENOM, INC.
Inventors:
Chen ZHAO, Cosmin DECIU, Dirk Johannes VAN DEN BOOM
Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.
Abstract: Techniques are described for identifying a genetic variant in a test sample by comparing sequences reads obtained from the test sample to unique k-mers that are representative of a target genomic region. In one particular aspect, a method is described that includes generating a dictionary of a target genomic region having a set of unique k-mers by: accessing a sequence of the target genomic region, determining a set of k-mers for the target genomic region, comparing the set of k-mers for the target genomic region with one or more sets of k-mers for non-target genomic regions, and selecting the unique k-mers that do not appear in the one or more sets of k-mers for non-target genomic regions. The dictionary can then be used to identify a genetic variant in a test sample by comparing sequences reads obtained from the test sample to the unique k-mers in the dictionary.
Type:
Application
Filed:
November 2, 2023
Publication date:
May 2, 2024
Applicant:
Sequenom, Inc.
Inventors:
Chen Zhao, Cosmin Deciu, Eyad Almasri, Tong Liu
Abstract: Provided herein are methods, compositions and kits to extract and relatively enrich by physical separation or amplification short base pair nucleic acid in the presence of a high background of genomic material (e.g., host or maternal nucleic acids).
Type:
Grant
Filed:
April 15, 2020
Date of Patent:
April 9, 2024
Assignee:
SEQUENOM, INC.
Inventors:
Carolyn R. Hoyal-Wrightson, Andreas Braun, Karsten E. Schmidt
Abstract: Technology provided herein relates in part to methods, processes, machines and apparatuses for non-invasive assessment of genetic alterations. In particular, a method is provided for that includes obtaining a set of sequence reads. The sequence reads each include a single molecule barcode (SMB) sequence that is a non-random oligonucleotide sequence. The method further includes assigning the sequence reads to read groups according to a read group signature. The read group signature comprises an SMB sequence and a start and end position of a nucleic acid fragment from the circulating cell free sample nucleic acid. The sequence reads comprising start and end positions and an SMB sequence similar to the read group signature are assigned to a read group. The method further includes generating a consensus for each read group, and determining the presence or absence of a genetic alteration based on the consensus for each read group.
Type:
Grant
Filed:
January 22, 2018
Date of Patent:
March 12, 2024
Assignee:
SEQUENOM, INC
Inventors:
Mostafa Azab, Michael Sykes, Youting Sun, Amin Mazloom, Taylor Jensen, Mathias Ehrich, Christopher Ellison
Abstract: Technology provided herein relates in part to methods, processes, machines and apparatuses for non-invasive assessment of copy number alterations. In particular, a method is provided for determining presence or absence of a copy number alteration for a test subject. The method includes providing a set of sequence reads. The sequence reads may be obtained from circulating cell free sample nucleic acid from a test sample obtained from the test subject, and the circulating cell free sample nucleic acid may be captured by probe oligonucleotides under hybridization conditions. The method further includes determining a probe coverage quantification of the sequence reads for the probe oligonucleotides and determining the presence or absence of a copy number alteration in the circulating cell free sample nucleic acid based on the probe coverage quantification of the sequence reads for the probe oligonucleotides for the test sample.
Type:
Grant
Filed:
January 22, 2018
Date of Patent:
March 12, 2024
Assignee:
SEQUENOM, INC
Inventors:
Yijin Wu, Amin Mazloom, Yang Zhong, Mostafa Azab
Abstract: Methods for non-invasive assessment of genetic variations that make use of nucleic acid fragment length information, in particular length of fragments in circulating cell-free nucleic acids and compares the number of counts from fragments with different length.
Type:
Grant
Filed:
July 29, 2015
Date of Patent:
October 10, 2023
Assignee:
SEQUENOM, INC
Inventors:
Chen Zhao, Cosmin Deciu, Dirk Johannes Van Den Boom
Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.
Abstract: Technology provided herein relates in part to methods, processes, machines and apparatuses for non-invasive assessment of genomic nucleic acid instability and genomic nucleic acid stability.
Type:
Application
Filed:
October 10, 2022
Publication date:
June 15, 2023
Applicant:
Sequenom, Inc.
Inventors:
Youting Sun, Sung Kyun Kim, Mathias Ehrich, Christopher Ellison, Taylor Jensen, Amin Mazloom