Patents Assigned to Sequenom, Inc.
  • Patent number: 10323268
    Abstract: Provided herein are methods, processes and apparatuses for non-invasive assessment of genetic variations.
    Type: Grant
    Filed: May 6, 2016
    Date of Patent: June 18, 2019
    Assignee: Sequenom, Inc.
    Inventors: Cosmin Deciu, Zeljko Jovan Dzakula, Mathias Ehrich, Sung Kyun Kim
  • Patent number: 10196681
    Abstract: Provided herein are methods, processes and apparatuses for determining the fraction of fetal nucleic acid in a test sample derived from a pregnant female with improved accuracy and/or precision. Also, provided herein are methods, processes and apparatuses for determining the presence or absence of a genetic variation in a fetus with improved accuracy and/or precision. Certain methods include using fetal fraction measurements for the determination of a fetal genetic variation.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: February 5, 2019
    Assignee: Sequenom, Inc.
    Inventors: Cosmin Deciu, Zeljko Dzakula, John Allen Tynan, Grant Hogg
  • Patent number: 10144966
    Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.
    Type: Grant
    Filed: April 15, 2015
    Date of Patent: December 4, 2018
    Assignee: Sequenom, Inc.
    Inventor: Charles R. Cantor
  • Patent number: 10093976
    Abstract: Systems, methods, and apparatus for determining at least a portion of fetal genome are provided. DNA fragments from a maternal sample (maternal and fetal DNA) can be analyzed to identify alleles at certain loci. The amounts of DNA fragments of the respective alleles at these loci can be analyzed together to determine relative amounts of the haplotypes for these loci and determine which haplotypes have been inherited from the parental genomes. Loci where the parents are a specific combination of homozygous and heterozygous can be analyzed to determine regions of the fetal genome. Reference haplotypes common in the population can be used along with the analysis of the DNA fragments of the maternal sample to determine the maternal and paternal genomes. Determination of mutations, a fractional fetal DNA concentration in a maternal sample, and a proportion of coverage of a sequencing of the maternal sample can also be provided.
    Type: Grant
    Filed: May 15, 2013
    Date of Patent: October 9, 2018
    Assignees: The Chinese University of Hong Kong, Sequenom Inc.
    Inventors: Yuk Ming Dennis Lo, Kwan Chee Chan, Wai Kwon Rossa Chiu, Charles Cantor
  • Publication number: 20180282801
    Abstract: Technology described herein pertains in part to diagnostic tests that make use of sequence reads generated by a sequencing process. In some embodiments, a component used to generate a chromosome representation can be based on counts of sequence reads not aligned to a reference genome.
    Type: Application
    Filed: April 27, 2018
    Publication date: October 4, 2018
    Applicant: Sequenom, Inc.
    Inventors: Chen Zhao, Cosmin Deciu
  • Patent number: 10053685
    Abstract: Provided herein are methods and compositions to extract and enrich by, physical separation or amplification, relatively short nucleic acids from a nucleic acid composition containing a high background of longer nucleic acids (e.g., host or maternal nucleic acids; genomic nucleic acid and the like).
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: August 21, 2018
    Assignee: SEQUENOM, INC.
    Inventors: Michele Elizabeth Wisniewski, William Hang Kwong, Firouz Mohsenian, Jian-Hua Ding
  • Publication number: 20180220176
    Abstract: The present application relates to a nucleic acid molecule having a nucleotide sequence encoding a bifunctional polypeptide comprising the DNA-binding domain of a protein belonging to the family of Methyl-CpG binding proteins (MBDs) and the Fc portion of an antibody. In addition, vectors and host cells which comprise said nucleic acid molecule and polypeptides which are encoded by said nucleic acid molecule as well as processes for producing said polypeptide are disclosed. Moreover, the present application provides an antibody specifically binding said polypeptide and compositions, in particular diagnostic compositions comprising the nucleic acid molecule(s), vector(s), host cell(s), polypeptide(s) or antibodie(s) of the present application. Furthermore, methods and uses employing the polypeptides of the present invention for detecting methylated DNA, in particular in tumorous tissue or tumor cells are provided.
    Type: Application
    Filed: January 22, 2018
    Publication date: August 2, 2018
    Applicant: Sequenom, Inc.
    Inventor: Michael Rehli
  • Patent number: 9984198
    Abstract: Provided herein are methods, processes and apparatuses for non-invasive assessment of genetic variations.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: May 29, 2018
    Assignee: SEQUENOM, INC.
    Inventors: Cosmin Deciu, Zeljko Dzakula, Mathias Ehrich, Taylor Jacob Jensen
  • Patent number: 9926593
    Abstract: Provided are methods for identifying the presence or absence of a chromosome abnormality by which a cell-free sample nucleic acid from a subject is analyzed. In certain embodiments, provided are methods for identifying the presence or absence of a fetal chromosome abnormality in a nucleic acid from cell-free maternal blood.
    Type: Grant
    Filed: December 20, 2010
    Date of Patent: March 27, 2018
    Assignee: SEQUENOM, INC.
    Inventors: Mathias Ehrich, Guy Del Mistro, Cosmin Deciu, Yong Qing Chen, Ron Michael McCullough, Roger Chan Tim
  • Patent number: 9920361
    Abstract: Technology provided herein relates in part to methods, processes, compositions and apparatuses for analyzing nucleic acid.
    Type: Grant
    Filed: March 1, 2013
    Date of Patent: March 20, 2018
    Assignee: SEQUENOM, INC.
    Inventors: Sung K. Kim, Cosmin Deciu
  • Patent number: 9873919
    Abstract: The present application relates to a nucleic acid molecule having a nucleotide sequence encoding a bifunctional polypeptide comprising the DNA-binding domain of a protein belonging to the family of Methyl-CpG binding proteins (MBDs) and the Fc portion of an antibody. In addition, vectors and host cells which comprise said nucleic acid molecule and polypeptides which are encoded by said nucleic acid molecule as well as processes for producing said polypeptide are disclosed. Moreover, the present application provides an antibody specifically binding said polypeptide and compositions, in particular diagnostic compositions comprising the nucleic acid molecule(s), vector(s), host cell(s), polypeptide(s) or antibodie(s) of the present application. Furthermore, methods and uses employing the polypeptides of the present invention for detecting methylated DNA, in particular in tumorous tissue or tumor cells are provided.
    Type: Grant
    Filed: June 9, 2015
    Date of Patent: January 23, 2018
    Assignee: Sequenom, Inc.
    Inventor: Michael Rehli
  • Patent number: 9850480
    Abstract: Provided herein are methods and compositions to extract and enrich by, physical separation or amplification, relatively short nucleic acids from a nucleic acid composition containing a high background of longer nucleic acids (e.g., host or maternal nucleic acids; genomic nucleic acid and the like).
    Type: Grant
    Filed: January 18, 2017
    Date of Patent: December 26, 2017
    Assignee: SEQUENOM, INC.
    Inventors: Michele Elizabeth Wisniewski, William Hang Kwong, Firouz Mohsenian, Jian-Hua Ding
  • Publication number: 20170342477
    Abstract: Technology provided herein relates in part to methods, processes, machines and apparatuses for detecting genetic variations. In some embodiments, the technology is related to non-invasive assessment of aneuploidies.
    Type: Application
    Filed: May 26, 2017
    Publication date: November 30, 2017
    Applicant: Sequenom, Inc.
    Inventors: Taylor Jacob Jensen, Mathias Ehrich, Dirk van den Boom, John Allen Tynan, Sung Kyun Kim, Timothy S. Burcham, Christopher K. Ellison, Youting Sun
  • Patent number: 9738931
    Abstract: Blood plasma of pregnant women contains fetal and (generally >90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ?500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of <500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ?500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.
    Type: Grant
    Filed: February 1, 2013
    Date of Patent: August 22, 2017
    Assignee: SEQUENOM, INC.
    Inventors: Sinuhe Hahn, Wolfgang Holzgreve, Bernhard Zimmermann, Ying Li
  • Patent number: 9605313
    Abstract: Technology provided herein relates in part to methods, processes and apparatuses for non-invasive assessment of genetic variations.
    Type: Grant
    Filed: March 1, 2013
    Date of Patent: March 28, 2017
    Assignee: SEQUENOM, INC.
    Inventors: Charles R. Cantor, Grace DeSantis, Reinhold Mueller, Mathias Ehrich
  • Patent number: 9580741
    Abstract: Provided herein are methods and compositions to extract and enrich by, physical separation or amplification, relatively short nucleic acids from a nucleic acid composition containing a high background of longer nucleic acids (e.g., host or maternal nucleic acids; genomic nucleic acid and the like).
    Type: Grant
    Filed: June 5, 2014
    Date of Patent: February 28, 2017
    Assignee: Sequenom, Inc.
    Inventors: Michele Elizabeth Wisniewski, William Hang Kwong, Firouz Mohsenian, Jian-Hua Ding
  • Patent number: 9580751
    Abstract: Blood plasma of pregnant women contains fetal and (generally >90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ?500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of <500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ?500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: February 28, 2017
    Assignee: SEQUENOM, INC.
    Inventors: Sinuhe Hahn, Wolfgang Holzgreve, Bernhard Zimmermann, Ying Li
  • Patent number: 9512480
    Abstract: Systems, methods, and apparatus for determining at least a portion of fetal genome are provided. DNA fragments from a maternal sample (maternal and fetal DNA) can be analyzed to identify alleles at certain loci. The amounts of DNA fragments of the respective alleles at these loci can be analyzed together to determine relative amounts of the haplotypes for these loci and determine which haplotypes have been inherited from the parental genomes. Loci where the parents are a specific combination of homozygous and heterozygous can be analyzed to determine regions of the fetal genome. Reference haplotypes common in the population can be used along with the analysis of the DNA fragments of the maternal sample to determine the maternal and paternal genomes. Determination of mutations, a fractional fetal DNA concentration in a maternal sample, and a proportion of coverage of a sequencing of the maternal sample can also be provided.
    Type: Grant
    Filed: May 15, 2013
    Date of Patent: December 6, 2016
    Assignees: The Chinese University of Hong Kong, Sequenom Inc.
    Inventors: Yuk Ming Dennis Lo, Kwan Chee Chan, Wai Kwun Rossa Chiu, Charles Cantor
  • Patent number: 9453257
    Abstract: Provided herein are methods, compositions and kits to extract and relatively enrich by physical separation or amplification short base pair nucleic acid in the presence of a high background of genomic material (e.g., host or maternal nucleic acids).
    Type: Grant
    Filed: February 14, 2014
    Date of Patent: September 27, 2016
    Assignee: SEQUENOM, INC.
    Inventors: Carolyn R. Hoyal-Wrightson, Andreas Braun, Karsten E Schmidt
  • Patent number: 9404150
    Abstract: Provided herein are products and processes for the amplification, detection and sequencing of short-stranded nucleic acid in the presence of a high background of long-stranded genomic material (e.g., host or maternal nucleic acids). The methods rely on the use of inside and outside primers introduced at varying concentrations, as well as universal amplification reactions that preferentially amplify short, low copy number nucleic acid.
    Type: Grant
    Filed: August 28, 2008
    Date of Patent: August 2, 2016
    Assignee: SEQUENOM, INC.
    Inventors: Min Seob Lee, Yanfeng Yang