Patents Assigned to Sigma-Aldrich Co. LLC
  • Publication number: 20190316102
    Abstract: Paired CRISPR nickase ribonucleoproteins engineered to target immune-related genomic loci and methods of using said ribonucleoproteins to modify the immune-related genomic loci.
    Type: Application
    Filed: April 12, 2019
    Publication date: October 17, 2019
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Qingzhou Ji, Gregory D. Davis, Jacob T. Lamberth
  • Patent number: 10434492
    Abstract: A composition, method and device for the preparation of biological samples for subsequent instrumental analyses, such as GC, GC-MS, LC and LC-MS analysis, using a solid phase extraction (SPE) process is described. Through SPE process alone or an integrated combination of protein precipitation, filtration, and SPE using a hydrophobic zirconia-coated chromatographic media, interfering compounds, such as proteins, glycerides and phosphate-containing compounds, are eliminated from the biological, food, environmental and biotechnology samples, affording an enhanced analyte response during the instrumental analysis.
    Type: Grant
    Filed: May 21, 2010
    Date of Patent: October 8, 2019
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Xiaoning Lu, Olga I. Shimelis, Maochun M. Ye
  • Patent number: 10377990
    Abstract: The present invention provides mammalian cell lines that have been genetically engineered causing such cell lines to be resistant to viral entry and/or propagation, and provides methods of using said cell lines to reduce or prevent viral contamination of biologic production systems.
    Type: Grant
    Filed: March 3, 2015
    Date of Patent: August 13, 2019
    Assignee: SIgma-Aldrich Co. LLC
    Inventors: Nan Lin, Joaquina Mascarenhas, Audrey Chang, David Onions, Henry George, Kevin Kayser
  • Publication number: 20190177780
    Abstract: Methods for visualizing modified nucleotides in a specific nucleic acid sequence or specific nucleic acid sequence interactions in single cells, wherein the methods comprise coupling an in situ hybridization (ISH) reaction with a proximity ligation assay (PLA) reaction.
    Type: Application
    Filed: February 22, 2019
    Publication date: June 13, 2019
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Vikas Palhan, Carol Kreader
  • Publication number: 20190144850
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Application
    Filed: October 15, 2018
    Publication date: May 16, 2019
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventor: Carol A. Kreader
  • Publication number: 20190136305
    Abstract: Methods are provided herein for identifying rare and/or unknown DNA sequences by next-generation sequencing approaches. Isolated double-stranded (ds), single-stranded (ss), or ds/ss DNA is fragmented and the fragments are polished, phosphorylated, and tailed, as necessary. Fragmentation can be enzymatic or mechanical. A universal adapter sequence is ligated to each fragment, wherein the adapter can have a top strand without a 5? phosphate, a 3? with an —H in place of the —OH, and/or a 3? extra base complementary to any base added to the polished fragments. The ligatamers may then serve as templates for ampli?cation using a forward primer complementary to the adapter sequence and a reverse primer targeted to the fragment sequence. Compositions produced by these methods and kits adapted for performing these methods are also described herein.
    Type: Application
    Filed: May 20, 2016
    Publication date: May 9, 2019
    Applicant: Sigma-Aldrich Co. LLC
    Inventor: Brian Ward
  • Patent number: 10266851
    Abstract: Compositions and methods for using programmable DNA binding proteins to increase the efficiency and/or specificity of targeted genome modification or to facilitate the detection of specific genomic loci in eukaryotic cells.
    Type: Grant
    Filed: February 20, 2017
    Date of Patent: April 23, 2019
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Fuqiang Chen
  • Patent number: 10253356
    Abstract: Methods for visualizing modified nucleotides in a specific nucleic acid sequence or specific nucleic acid sequence interactions in single cells, wherein the methods comprise coupling an in situ hybridization (ISH) reaction with a proximity ligation assay (PLA) reaction.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: April 9, 2019
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Vikas Palhan, Carol Kreader
  • Patent number: 10227610
    Abstract: Methods and compositions for increasing nuclease-mediated genomic modification using DNA repair inhibitors are provided.
    Type: Grant
    Filed: February 24, 2014
    Date of Patent: March 12, 2019
    Assignees: Sangamo Therapeutics, Inc., Sigma Aldrich Co LLC
    Inventors: Fuqiang Chen, Qiaohua Kang, Thomas Wechsler
  • Publication number: 20180340221
    Abstract: The present disclosure provides reagents and methods for molecular proximity detection of specific endogenous nucleic acids in situ using RNA-guided nucleic acid binding proteins.
    Type: Application
    Filed: September 23, 2016
    Publication date: November 29, 2018
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Gregory D Davis, Vikas B Palhan, Carol A. Kreader
  • Patent number: 10131902
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: November 20, 2018
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Carol Kreader
  • Patent number: 9958422
    Abstract: This disclosure provides methods and devices for quantitating, separating and/or detecting water in a liquid, gas or solid sample comprising one or more chemicals, the method comprising: providing the liquid, gas or solid sample comprising water and the one or more chemicals; and exposing said liquid, gas or solid sample to at least one solid support including at least one dicationic and/or tricationic species of Formula I or II adsorbed, absorbed or immobilized on the solid support.
    Type: Grant
    Filed: October 22, 2015
    Date of Patent: May 1, 2018
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Daniel W. Armstrong
  • Publication number: 20180044723
    Abstract: Processes, oligonucleotides, and kits for amplifying RNA. In particular, the processes generate and amplify cDNA libraries in which the orientation of the input RNA molecule is preserved in the products. Among the various aspects of the present disclosure is the provision of process for directionally amplifying RNA. The process comprises reverse transcribing at least one RNA molecule in the presence of a plurality of first synthesis primers to generate a plurality of first strands of complementary DNA (cDNA), wherein each of the first synthesis primers comprises a 3? sequence having complementarity to a portion of the RNA molecule, a non-complementary 5? sequence corresponding to one or more amplification primers, and optionally an internal tag sequence comprising a first tag sequence.
    Type: Application
    Filed: March 24, 2016
    Publication date: February 15, 2018
    Applicant: Sigma-Aldrich Co. LLC
    Inventors: Kenneth B Heuermann, Carol A Kreader, Jaime K Robert, Brian W Ward
  • Publication number: 20180037946
    Abstract: Processes and kits for preparing a plurality of amplification products with reduced non-specific amplification artifacts.
    Type: Application
    Filed: March 4, 2016
    Publication date: February 8, 2018
    Applicant: Sigma-Aldrich Co. LLC
    Inventors: Timothy Seebeck, Fuqiang Chen, Brian W Ward
  • Publication number: 20170362647
    Abstract: Methods for visualizing modified nucleotides in a specific nucleic acid sequence or specific nucleic acid sequence interactions in single cells, wherein the methods comprise coupling an in situ hybridization (ISH) reaction with a proximity ligation as say (PLA) reaction.
    Type: Application
    Filed: December 22, 2015
    Publication date: December 21, 2017
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Vikas Palhan, Carol Kreader
  • Patent number: 9671393
    Abstract: The present invention provides isolated, non-viable bacterial cells comprising a plurality of nucleic acid crosslinks, methods for making said cells, and methods for using said cells to isolate DNA-protein complexes. In particular, the nucleic acids of the cells are crosslinked by contacting the cells with a furocoumarin compound and ultraviolet light.
    Type: Grant
    Filed: September 2, 2011
    Date of Patent: June 6, 2017
    Assignee: SIGMA-ALDRICH CO., LLC
    Inventors: Vikas B. Palhan, Carol Kreader, Ernie Mueller
  • Patent number: 9670271
    Abstract: The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.
    Type: Grant
    Filed: June 2, 2016
    Date of Patent: June 6, 2017
    Assignee: SIGMA-ALDRICH CO. LLC
    Inventors: Nan Lin, Natalie Sealover, Henry George, Kevin Kayser
  • Publication number: 20170051354
    Abstract: The present disclosure provides genetically engineered cell lines comprising chromosomally integrated synthetic sequences having predetermined epigenetic modifications, wherein a predetermined epigenetic modification is correlated with a known diagnosis, prognosis or level of sensitivity to a disease treatment. Also provided are kits comprising said epigenetically modified synthetic nucleic acids or cells comprising said epigenetically modified synthetic nucleic acids that can be used as reference standards for predicting responsiveness to therapeutic treatments, diagnosing diseases, or predicting disease prognosis.
    Type: Application
    Filed: April 24, 2015
    Publication date: February 23, 2017
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Gregory D. DAVIS, Qiaohua KANG
  • Publication number: 20160376582
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Application
    Filed: November 25, 2014
    Publication date: December 29, 2016
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventor: Carol KREADER
  • Patent number: 9528143
    Abstract: A composition suitable for formulation of an enzymatic reaction mixture, the composition comprising a reaction component essential for an ex-vivo non-polymerase enzymatic reaction in which a substrate is catalyzed by an enzyme in a reaction mixture to form a product, and a tracer compatible with the enzyme, the composition being substantially free of the substrate.
    Type: Grant
    Filed: April 12, 2007
    Date of Patent: December 27, 2016
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Brian W. Ward, David M. Ornitz, Michael G. Deines, Thomas F. Bittick