Patents Assigned to South Dakota State University
-
Patent number: 8669225Abstract: Methods are described for producing non-immunogenic nanoparticles from protein sources by controlling the pH in a nanoprecipitation process. The nanoparticles that are produced by the disclosed methods range in diameter size from about 100 ran to about 400 nm, with a preferred diameter size of from approximately 100 nm to approximately 300 nm, thereby rendering them non-immunogenic. The invention further discloses methods for producing nanoconjugates that are suitable for a variety of therapeutic, diagnostic and other uses.Type: GrantFiled: May 11, 2009Date of Patent: March 11, 2014Assignee: South Dakota State UniversityInventors: Omathanu P. Perumal, Satheesh K. Podaralla, Radhey S. Kaushik
-
Publication number: 20130210654Abstract: Methods for detecting at least seven cytokines in a porcine biological sample are provided. Also provided are multiplex assay kits that allow for the detection and quantification of the cytokines in a single reaction mixture. Use of the methods and kits for diagnosis, prognosis, and monitoring of immunity is also contemplated.Type: ApplicationFiled: June 9, 2011Publication date: August 15, 2013Applicant: SOUTH DAKOTA STATE UNIVERSITYInventors: Jane Christopher-Hennings, Steven Lawson, Eric Nelson, Ying Fang, Joan K. Lunney
-
Publication number: 20130202737Abstract: The present disclosure relates to methods and compositions for preparing cheese products with exopolysaccharide (EPS). In particular, an EPS-producing culture is deactivated either before or shortly after combination of the EPS-fortified ingredient with a base milk ingredient, i.e. cheese milk. In many cases, the base milk ingredient is a low or reduced fat ingredient. The base milk ingredient may have an added fat. Also contemplated are cheese products made using the disclosed methods and compositions.Type: ApplicationFiled: May 21, 2011Publication date: August 8, 2013Applicant: SOUTH DAKOTA STATE UNIVERSITYInventor: Ashraf Hassan
-
Patent number: 8475864Abstract: A method of reversing the formation of an oxidized off-flavor in milk that includes providing milk, and heating the milk to a temperature between approximately 70° C. and approximately 90° C. for a period of between approximately 25 seconds and approximately 60 seconds.Type: GrantFiled: December 16, 2004Date of Patent: July 2, 2013Assignee: South Dakota State UniversityInventors: Robert J. Baer, Howard H. Bonnemann
-
Patent number: 8420101Abstract: Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic Escherichia coli. The vaccine may include an Escherichia coli strain. The Escherichia coli strain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of Escherichia coli. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain of Escherichia coli with the genetic fusion.Type: GrantFiled: March 16, 2011Date of Patent: April 16, 2013Assignee: South Dakota State UniversityInventors: David Francis, Weiping Zhang
-
Publication number: 20120276637Abstract: The disclosed embodiments provide cyanobacteria spp. that have been genetically engineered to have increased production of carbon-based products of interest. These genetically engineered hosts efficiently convert carbon dioxide and light into carbon-based products of interest such as long chained hydrocarbons. Several constructs containing polynucleotides encoding enzymes active in the metabolic pathways of cyanobacteria are disclosed. In many instances, the cyanobacteria strains have been further genetically modified to optimize production of the carbon-based products of interest. The optimization includes both up-regulation and down-regulation of particular genes.Type: ApplicationFiled: February 24, 2012Publication date: November 1, 2012Applicant: SOUTH DAKOTA STATE UNIVERSITYInventors: RUANBAO ZHOU, WILLIAM GIBBONS
-
Publication number: 20120269842Abstract: The disclosure relates to fusions of enterotoxigenic Escherichia coli (ETEC) polynucleotides and polypeptides. In one example, a toxiod heat-labile (LT) and heat-stable (STa) fusion is described. This fusion may include LT with a substituted amino acid at position 192, a linker, and STa with a substituted amino acid at positions 11, 12, 13, or 14. Further provided are vaccines containing the disclosed fusions. Example methods for administering to a subject the disclosed vaccines and using the fusions and vaccines to reduce or eliminate contamination of a food or water supply are additionally contemplated.Type: ApplicationFiled: October 8, 2010Publication date: October 25, 2012Applicant: SOUTH DAKOTA STATE UNIVERSITYInventor: Weiping Zhang
-
Patent number: 8182984Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major problem in the pork industry worldwide. The inclusion of markers in vaccines will allow for diagnostic differentiation of vaccinated animals from those naturally infected with wild-type virus. Using a cDNA infectious clone of North American Type 1 PRRSV, a recombinant green fluorescent protein (GFP) tagged PRRSV has been made, containing deletion of an immunogenic epitope, ES4, in the nsp2 region. GFP and ES4 epitope-based ELISAs compliment the marker identification.Type: GrantFiled: June 24, 2008Date of Patent: May 22, 2012Assignee: South Dakota State UniversityInventors: Ying Fang, Eric A. Nelson, Jane Hennings
-
Publication number: 20120040335Abstract: An enzyme-linked immunosorbent assay (ELISA) is based on the non-structural protein 7 (nsp7) of porcine reproductive and respiratory syndrome virus (PRRSV) and provides for the simultaneous detection and differentiation of serum antibodies directed against Type 1 (European) and Type 2 (North American) PRRSV. The invention provides a serological assay for the detection and/or differentiation of serum antibodies directed against Type 1 and/or Type 2 PRRSV utilizing PRRSV nsp7 as an antigen, and provides a diagnostic method for the detection of PRRSV infection, epidemiological surveys, and outbreak investigations. The invention may be used either alone or as a follow-up assay to determine the true status of unexpected positive results that may occur using other assays, such as the IDEXX HERDCHEK PRRS ELISA.Type: ApplicationFiled: November 25, 2009Publication date: February 16, 2012Applicant: SOUTH DAKOTA STATE UNIVERSITYInventors: Ying Fang, Elizabeth M. Brown, Craig Welbon, Eric A. Nelson
-
Publication number: 20110318379Abstract: Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic Escherichia coli. The vaccine may include an Escherichia coli strain. The Escherichia coli strain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of Escherichia coli. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain of Escherichia coli with the genetic fusion.Type: ApplicationFiled: March 16, 2011Publication date: December 29, 2011Applicant: SOUTH DAKOTA STATE UNIVERSITYInventors: DAVID FRANCIS, WEIPING ZHANG
-
Patent number: 7927586Abstract: Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic Escherichia coli. The vaccine may include an Escherichia coli strain. The Escherichia coli strain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of Escherichia coli. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain of Escherichia coli with the genetic fusion.Type: GrantFiled: July 8, 2008Date of Patent: April 19, 2011Assignee: South Dakota State UniversityInventors: David Francis, Weiping Zhang
-
Publication number: 20100136047Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major problem in the pork industry worldwide. The inclusion of markers in vaccines will allow for diagnostic differentiation of vaccinated animals from those naturally infected with wild-type virus. Using a cDNA infectious clone of North American Type 1 PRRSV, a recombinant green fluorescent protein (GFP) tagged PRRSV has been made, containing deletion of an immunogenic epitope, ES4, in the nsp2 region. GFP and ES4 epitope-based ELISAs compliment the marker identification.Type: ApplicationFiled: June 24, 2008Publication date: June 3, 2010Applicant: South Dakota State UniversityInventors: Ying Fang, Eric A. Nelson, Jane Hennings
-
Publication number: 20100008949Abstract: Vaccines and methods for making and using the same. An example vaccine may be a vaccine against enterotoxigenic Escherichia coli. The vaccine may include an Escherichia coli strain. The Escherichia coli strain may produce K88 fimbria and a fusion protein including a mutant LT enterotoxin linked with a STb enterotoxin. An example method for producing a vaccine for porcine post-weaning diarrhea may include providing a first strain of Escherichia coli. The strain may include the eltAB gene and the estB gene. The method may also include amplifying the eltAB gene, mutating the eltAB gene, generating a genetic fusion of the mutant eltAB gene with the estB gene, and transforming a second strain of Escherichia coli with the genetic fusion.Type: ApplicationFiled: July 8, 2008Publication date: January 14, 2010Applicant: SOUTH DAKOTA STATE UNIVERSITYInventors: David Francis, Weiping Zhang
-
Patent number: 7264804Abstract: The invention includes kits for producing and using immunogenic compositions that include swine infertility and respiratory virus. The invention also includes vaccines and sera for treatment of Mystery Swine Disease (MSD), a method for producing the vaccine, methods for diagnosis of MSD, a viral agent that will mimic “mystery swine disease” and antibodies to the viral agent useful in diagnosis and treatment of MSD. The serum contains mammalian antibodies which are effective in treating MSD.Type: GrantFiled: May 12, 2005Date of Patent: September 4, 2007Assignees: Boehringer Ingleheim Vetmedica, Inc., Regents of the University of Minnesota, South Dakota State UniversityInventors: James E. Collins, David A. Benfield, Danny W. Chladek, Louis L. Harris, David E. Gorcyca
-
Publication number: 20070166735Abstract: Methods for the in vitro propagation of infectious prions (PrPSc) are provided. Follicular dendritic cells (FDCs) are cultured with B cells and infected with prions. Methods of detecting infectious prions (PrPSc) in an animal or human are also provided. Peripheral blood B cells are collected from an animal or human suspected of being infected with infections prions, cultured with follicular dendritic cells, and the presence of infectious prions is detected.Type: ApplicationFiled: December 1, 2006Publication date: July 19, 2007Applicant: SOUTH DAKOTA STATE UNIVERSITYInventor: ALAN YOUNG
-
Publication number: 20070099852Abstract: Cucurbitacins, cucurbitacin derivatives, and methods for making and using the same are provided.Type: ApplicationFiled: August 31, 2006Publication date: May 3, 2007Applicant: SOUTH DAKOTA STATE UNIVERSITYInventors: Fathi Halaweish, Judit Bartalis
-
Publication number: 20070049538Abstract: Cucurbitacins, cucurbitacin derivatives, and methods for making and using the same are provided.Type: ApplicationFiled: August 31, 2006Publication date: March 1, 2007Applicant: SOUTH DAKOTA STATE UNIVERSITYInventors: FATHI HALAWEISH, JUDIT BARTALIS
-
Patent number: 6855315Abstract: The invention includes a vaccine and sera for treatment of Mystery Swine Disease (MSD), a method for producing the vaccine, methods for diagnosis of MSD, a viral agent that will mimic “mystery swine disease” and antibodies to the viral agent useful in diagnosis and treatment of MSD. The serum contains mammalian antibodies which are effective in treating MSD.Type: GrantFiled: October 28, 2002Date of Patent: February 15, 2005Assignees: Regents of the University of Minnesota, South Dakota State University, Boehringer Ingelheim Vetmedica, Inc.Inventors: James E. Collins, David A. Benfield, Danny W. Chladek, Louis L. Harris, David E. Gorcyca
-
Publication number: 20040101612Abstract: A process for the manufacture of a salt whey product is provided. In the dried form the product contains 35-45% salt and 5-15% whey protein and in the liquid form, over 12% salt and over 30% solids. The salt whey product can be used as an ingredient in the manufacture of process cheese products and other food products. The salt whey product provides important functional and nutritional attributes from whey proteins.Type: ApplicationFiled: November 27, 2002Publication date: May 27, 2004Applicant: South Dakota State UniversityInventor: Vikram V. Mistry
-
Patent number: 6498008Abstract: The invention includes a vaccine and sera for treatment of Mystery Swine Disease (MSD), a method for producing the vaccine, methods for diagnosis of MSD, a viral agent that will mimic “mystery swine disease” and antibodies to the viral agent useful in diagnosis and treatment of MSD. The serum contains mammalian antibodies which are effective in treating MSD.Type: GrantFiled: December 18, 2000Date of Patent: December 24, 2002Assignees: Regents of the University of Minnesota, South Dakota State University, Boehringer Ingelheim Vetmedica, Inc.Inventors: James E. Collins, David A. Benfield, Danny W. Chladek, Louis L. Harris, David E. Gorcyca