Abstract: A system for determining a position of a probe relative to an object such as a head of a body of a patient. The head includes a surface such as a forehead having a contour. Cross sectional images of the head are scanned and stored as a function of the forehead contour. If the forehead contour does not appear in the scan images, then the position of the forehead contour relative to the scan images is determined with an optical scanner and a ring. During surgery, the optical scanner also determines the position of the forehead relative to the ring. An array for receiving radiation emitted from the probe and from the ring generates signals indicating the position of the tip of the probe relative to the ring. A stereotactic imaging system generates and displays an image of the head corresponding to the measured position of the tip of the probe. The system may also display scan images from different scanning technologies which scan images correspond to the same position in the head.

Abstract: The present invention is based upon the observation that the temperature sensitive phenotype of the cp45 strain of HPIV-3 correlates to a mutation in the large, or L, gene of cp45 relative to the corresponding gene in the wild-type strain. This correlation enables new vaccines directed at viruses other than HPIV-3 by combining, through genetic engineering methods, the region of the cp45 viral genome which encodes proteins responsible for replication and internal structure with the region of the genome of the target virus which encodes proteins responsible for attachment, penetration and release of the virus and virus progeny, respectfully. Moreover, it is possible to determine whether HPIV-3 or a cp45-hybrid virus is attenuated by confirming the presence or absence of mutations in its L gene.

Abstract: Adenovirus E1B 19 kD protein protects against cell death induced by viral infection and certain external physical and chemical stimuli. Activity of the 19 kD protein is similar to the cell death suppressing activity of the protein coded by the Bcl-2 protooncogene. Bcl-2 protein can functionally substitute for the E1B 19 kD protein during adenovirus infection and in transformation of primary cells with adenovirus E1A. Five different cDNA's for proteins, designated Nip1, Nip2, Nip3, Bip1A and Bip13 that specifically interact with the 19 kD protein were found. Mutational analysis of the interaction indicates that at least four of the proteins (Nip1, Nip2, Nip3 and Bip1A) associate with 19 kD protein at specific sites thereof. Homologous motifs are found on Bcl2. An additional protein, (Bip5), interacts with Bcl-2 but not with the 19K protein.

Abstract: A system for determining a position of a probe (302) relative to an object such as a head (390) of a body of a patient. The head includes a surface such as a forehead (394) having a contour. The head is placed in a cradle (392) equipped with an arc (393). The cross sectional images of the head are determined relative to the arc. A hand held unit (380) optically scans the forehead and the arc. During scanning to generate the cross sectional images, the optical scanner (380) is used to determine the position of the forehead (394) relative to the cradle (392). During surgery, the optical scanner (380) also determines the position of the forehead (394) relative to a base ring (306). An array (300) for receiving radiation emitted from the probe (302) and from the base ring (306) generates signals indicating the position of the tip of the probe (302) relative to the base ring (306).

Abstract: Method for screening a sample oligonucleotide for a variation in sequence at a predetermined position thereof relative to a nucleic acid the sequence of which is known, wherein the sample oligonucleotide is provided as a single stranded molecule, the single stranded molecule is mixed with an inducing agent, a labeled nucleotide, and a primer having a sequence identical to a region flanking the predetermined position to form a mixture, the mixture having an essential absence of nucleotides constituted of bases other than the base of which the labeled nucleotide is constituted, the mixture is subjected to conditions conducive for the annealing of the primer to the single stranded molecule and the formation of a primer extension product incorporating the labeled nucleotide, and the mixture is analyzed for the presence of primer extension product containing labeled nucleotide.

Abstract: A domain of Bcl-2 that suppresses apoptosis by allowing cell survival permits cell proliferation when mutated. The wild type domain includes amino acid residues 51 to 97 (SEQ ID NO:13) of Bcl-2. Peptides including the domain and nucleotides encoding the domain are useful in molecular screening of human tumors for the presence of mutations that allow proliferation of cells that were otherwise marked for apoptosis. The peptides are also useful to screen for proteins that play a role in the modulation of cellular proliferation.

Abstract: The invention relates to novel Haemophilus adhesion proteins, nucleic acids, and antibodies.

Abstract: High molecular weight surface proteins of non-typeable Haemophilus inflenzae which exhibit immunogenic properties and genes encoding the same are described. Specifically, genes coding for two immunodominant high molecular weight proteins, HMW1 and HMW2, have been cloned, expressed and sequenced, while genes coding for high molecular proteins HMW3 and HMW4 have been cloned, expressed and partially sequenced.

Abstract: High molecular weight surface proteins of non-typeable Haemophilus influenzae which exhibit immunogenic properties and genes encoding the same are described. Specifically, genes coding for two immunodominant high molecular weight proteins, HMW1 and HMW2, have been cloned, expressed and sequenced, while genes coding for high molecular proteins HMW3 and HMW4 have been cloned, expressed and partially sequenced.

Abstract: A system for indicating a position of a tip of a probe which is positioned within an object on cross-sectional, scanned images of the object. The object includes reference points and the images of the object include reference images corresponding to the reference points. An array for receiving radiation emitted from the probe and from the reference points is digitized by a three dimensional digitizer to measure the position of the tip of the probe relative to the reference points of the object. A computer employing translational software translates the position of the tip of the probe into a coordinate system corresponding to the coordinate system of the cross-sectional images. A stereotactic imaging system selects the image of the object closest to the measured position of the tip of the probe and displays the selected image and a cursor representing the position of the tip of the probe on the selected images.

Abstract: An apparatus for correcting the curvature of a cornea of an eye comprises a housing for placement on the cornea for altering the curvature of cornea to extend a portion of the cornea above a predefined plane parallel to a plane tangent to the eye. The apparatus also includes a laser which is positioned to ablate the portion of the cornea which extends above the plane from the side of the cornea. A keratometer is also included to scan the cornea to determine the amount of the cornea extending above the plane.

Abstract: A transducer housing stabilization device for supporting a transcranial Doppler transducer against a temporal bone of a head. The device includes a generally planar, rigid member having an opening therein. The member has perforations therein for engaging an adhesive to secure the planar member adjacent to the temporal bone of the head. A transducer housing on the member receives the transducer thereby positioning the transducer in place adjacent the temporal bone of the head.

Abstract: A Judkins-type angiographic catheter adapted to be inserted in a coronary artery for measuring the velocity of coronary blood flow, comprising a tube of flexible relatively soft material having a proximal end and a distal end terminating in a tip, a Doppler crystal at the tip of the tube, and a conductor connected to the Doppler crystal and extending lengthwise of the tube. A connector at the proximal end of the catheter connects the conductor to a source of energy to cause the Doppler crystal to emit pulses at predetermined frequency for measuring the velocity of coronary blood flow. The Doppler crystal is relatively small compared to the diameter of said tube and is so located relative to the tube that the crystal is adapted to assume a position generally at the diametrical center of the coronary artery when the tip of the catheter is inserted therein.

Abstract: A catheter with a tip manipulation feature, comprising an outer tube of flexible resilient material having a proximal end and a distal end terminating in a tip. The interior of the tube is divided into first and second lumens extending lengthwise of the tube, the first lumen being adapted for passage of fluid such as blood therethrough. A pull line extends lengthwise of the tube inside the second lumen and exits the second lumen through an opening in the tube wall proximal to the tip of the tube. From this opening the pull line extends along the tube wall on the outside of the tube in a direction toward the tip of the tube and is attached to the outside of the wall of the tube adjacent the tip of the tube whereby tensioning of the pull line bends the tip of the tube thereby to manipulate it into a desired position. The tip resiliently returns to its original unstressed shape when the tension on the pull line is released.

Abstract: Substantially nontoxic materials such as cysteamine constituting a source of sulfhydryl groups are useful in inhibiting diarrhea induced by Escherichia coli heat-stable enterotoxin. Such materials block ST-induced secretion prior to the formation of cyclic GMP without interference with normal basal activity or metabolism and may inactivate ST itself by breaking disulfide bridges. Combinations of a substantially nontoxic material constituting a source of sulfhydryl groups and an alpha-2 adrenergic receptor, such as naphazoline, or berberine are also useful in inhibiting such diarrhea.

Abstract: Substantially nontoxic materials such as cysteamine constituting a source of sulfhydryl groups are useful in inhibiting diarrhea induced by Escherichia coli heat-stable enterotoxin. Such materials block ST-induced secretion prior to the formation of cyclic GMP without interference with normal basal activity or metabolism and may inactivate ST itself by breaking disulfide bridges.

Abstract: A functional extrachromosomal element capable of replication in filamentous fungi is provided. The extrachromosomal element employs (1) a mitochondrial replicating element or (2) a lower organism replication sequence recognized by the fungus, in combination with foreign DNA to provide replication, transcription, and translation of foreign regulatory elements and genes. The extrachromosomal element is exemplified by a mitochondrial replicating system from Neurospora.The cell strain E. coli HB101 containing the plasmid pALS-1-1 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39157.The cell strain E. coli HB101 containing the plasmid pALS-2 has been deposited at the A.T.C.C. on July 13, 1982, for patent purposes and given the designation ATCC 39158.

Abstract: Apparatus for controlling the redox potential of a perfusate for use in the preservation of organs to be transplanted and the like, which apparatus includes first and second cells, a membrane with a submicron pore size disposed in openings between the cells, first and second electrodes disposed in the first and second cells respectively, a reference electrode adapted to be in electrical contact with the perfusate, and circuitry for detecting the redox potential of the perfusate as measured against the reference electrode and for maintaining the redox potential at a predetermined level by causing current to flow between the first and second electrodes through the perfusate when the measured redox potential differs from the predetermined level. The first cell has first and second ports for ingress and egress of the perfusate into and out of the first cell.

Abstract: A new antibiotic named pamamycin is derived from S. alboniger. The antibiotic compound is neutral and terpene-like as derived from S. alboniger. The antibiotic is produced in conjunction with substances which either stimulate or inhibit the transition of substrate to aerial mycelia. Pamamycin may be separated by thin layer chromatography. The antibiotic displays particular R.sub.f values in thin layer chromatography as well as a particular antimicrobial spectrum for its use. The molecular formula is 621 and the elemental formula thereof is C.sub.36 H.sub.63 NO.sub.7 as determined by mass spectroscopy. The structure is believed to be a saturated alkane and terpene-like. In vivo tests with Staphylococcus aureus indicate that pamamycin is a potent inhibitor of both DNA and RNA synthesis and this antibiotic has present utility in a 1% solution to degerminate hospital walls.