Patents Assigned to StemCell Technologies Inc.
  • Patent number: 12005436
    Abstract: Surfaces contactable by a bulk liquid for use in particle isolation are described. The surfaces comprise a plurality of ribs spaced apart to capillarily retain a portion of the bulk liquid and particles of interest therebetween. Collectively, the portion of the bulk liquid retained by the plurality of ribs on the surface forms a liquid film. The one or more particles received within the space and enveloped by the liquid film may be protected from one or more forces exerted by a draining meniscus passing over the surface.
    Type: Grant
    Filed: May 25, 2018
    Date of Patent: June 11, 2024
    Assignee: StemCell Technologies Inc.
    Inventors: Eric Jervis, Tom Glawdel
  • Patent number: 11325984
    Abstract: The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.
    Type: Grant
    Filed: August 22, 2018
    Date of Patent: May 10, 2022
    Assignee: StemCell Technologies Inc.
    Inventor: Andy Isamu Kokaji
  • Publication number: 20200114345
    Abstract: Surfaces contactable by a bulk liquid for use in particle isolation are described. The surfaces comprise a plurality of ribs spaced apart to capillarily retain a portion of the bulk liquid and particles of interest therebetween. Collectively, the portion of the bulk liquid retained by the plurality of ribs on the surface forms a liquid film. The one or more particles received within the space and enveloped by the liquid film may be protected from one or more forces exerted by a draining meniscus passing over the surface.
    Type: Application
    Filed: May 25, 2018
    Publication date: April 16, 2020
    Applicant: Stemcell Technologies Inc.
    Inventors: Eric Jervis, Tom Glawdel
  • Patent number: 10081793
    Abstract: The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: September 25, 2018
    Assignee: StemCell Technologies Inc.
    Inventor: Andy Isamu Kokaji
  • Patent number: 9970849
    Abstract: An improved method for the preparation of target specific surfaces and uses thereof is described. In particular, the surfaces are bound to mono-specific tetrameric antibody complexes prior to their addition to a sample containing target entities and separating them from non-target entities.
    Type: Grant
    Filed: January 21, 2015
    Date of Patent: May 15, 2018
    Assignee: StemCell Technologies Inc.
    Inventor: Andy Isamu Kokaji
  • Patent number: 9708582
    Abstract: The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: July 18, 2017
    Assignee: Stemcell Technologies Inc.
    Inventors: Alexandra A. Blak, Sharon A. Louis, Michael John Riedel
  • Patent number: 9701935
    Abstract: A magnetic particle comprises a polysaccharide matrix and a plurality of magnetic crystals dispersed in the matrix. A method for making magnetic particles comprises combining a basic solution with a metal ion solution and allowing the metal ions to oxidize to form magnetic crystals, and combining the magnetic crystals with a polysaccharide solution to form the magnetic particles.
    Type: Grant
    Filed: April 16, 2009
    Date of Patent: July 11, 2017
    Assignee: StemCell Technologies Inc.
    Inventors: Steven M. Woodside, Graeme Milton, Jason Dowd
  • Patent number: 9683983
    Abstract: An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.
    Type: Grant
    Filed: May 3, 2012
    Date of Patent: June 20, 2017
    Assignee: StemCell Technologies Inc.
    Inventor: Steven M. Woodside
  • Patent number: 9260684
    Abstract: A cell culture device comprises a well. A plurality of microwells are within the well, and a first common fluid volume is within the well above the microwells. A set of sub-microwells are within each microwell, and a second common fluid volume is within each microwell above the set of sub-microwells.
    Type: Grant
    Filed: November 10, 2011
    Date of Patent: February 16, 2016
    Assignee: Stemcell Technologies Inc.
    Inventors: Oliver Egeler, Steven Woodside
  • Publication number: 20160032248
    Abstract: The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.
    Type: Application
    Filed: March 12, 2014
    Publication date: February 4, 2016
    Applicant: StemCell Technologies, Inc.
    Inventors: Brenton John SHORT, Christopher Duronio
  • Publication number: 20150352546
    Abstract: A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 75 degrees and 110 degrees.
    Type: Application
    Filed: January 10, 2014
    Publication date: December 10, 2015
    Applicant: Stemcell Technologies, Inc
    Inventors: Oliver Egeler, Steven Woodside
  • Publication number: 20140087360
    Abstract: An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.
    Type: Application
    Filed: May 3, 2012
    Publication date: March 27, 2014
    Applicant: Stemcell Technologies, Inc.
    Inventor: Steven M. Woodside
  • Patent number: 8409873
    Abstract: A method of pipetting comprises (a) providing a pipette tip holder having a plurality of segregated compartments and a tip support member for supporting a plurality of pipette tips in the pipette tip holder, the tip support member configured to support a single pipette in tip each segregated compartment; (b providing a plurality of pipette tips in the tip support member; (c) removing a first pipette tip from a first segregated compartment; (d) using the first pipette tip to complete a pipetting operation and placing the first pipette tip into a segregated compartment that has not contained a used pipette tip; (e) subsequently removing the first pipette tip from the segregated compartment and using the first pipette tip to complete another pipetting operation; and, (f) replacing the first pipette tip into the pipette tip holder, whereby the first pipette tip, when placed in the segregated compartment, is segregated in the pipette tip holder to reduce the possibility of a fluid from the first pipette tip contac
    Type: Grant
    Filed: October 9, 2009
    Date of Patent: April 2, 2013
    Assignee: Stemcell Technologies Inc.
    Inventors: Steven Woodside, Terry Thomas, Graeme Cross, Andrew Angus
  • Patent number: 7939031
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip. The seating surfaces are frustoconical outer surfaces. The pipette nozzle may be further configured to sealingly mate with and removably receive a third pipette tip having a third diameter different from the first diameter and the second diameter.
    Type: Grant
    Filed: August 11, 2008
    Date of Patent: May 10, 2011
    Assignee: Stemcell Technologies, Inc.
    Inventors: Andrew Angus, Adam Donath, Richard Grant
  • Patent number: 7819029
    Abstract: There is also provided an apparatus for use on an automated pipette machine for transmitting pressure changes produced by a pump on the machine to a pipette nozzle. The apparatus comprises a housing defining a chamber a first conduit and a second conduit. The first conduit extends into the chamber and has a first opening positioned in the chamber. The first conduit is fluidically connectible to the nozzle. The second conduit extends into the chamber and has a first opening positioned in the chamber. The second conduit is fluidically connectible to the pump. The first opening of the first conduit is positioned above the first opening of the second conduit. The chamber defines at least a selected volume between the height of the first opening of the first conduit and the height of the first opening of the second conduit.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: October 26, 2010
    Assignee: Stemcell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek
  • Patent number: 7814805
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough and having at least two seating surfaces provided on the body. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to correspondingly receive, sealingly mate and provide passage first end fluid communication with a second size of pipette tip.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: October 19, 2010
    Assignee: Stemcell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek
  • Patent number: 7723047
    Abstract: The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD35 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f or CD24 and CD14. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f or CD24 and CD14, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated.
    Type: Grant
    Filed: June 6, 2007
    Date of Patent: May 25, 2010
    Assignees: Stemcell Technologies Inc., British Columbia Cancer Agency Branch
    Inventors: John Stingl, Connie J. Eaves
  • Patent number: 7638285
    Abstract: The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD35 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated. The invention also relates to kits for carrying out this method and to the cell preparations prepared by this method.
    Type: Grant
    Filed: June 7, 2006
    Date of Patent: December 29, 2009
    Assignees: StemCell Technologies Inc., British Columbia Cancer Agency Branch
    Inventors: John Stingl, Connie J. Eaves
  • Publication number: 20090007703
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip.
    Type: Application
    Filed: June 30, 2008
    Publication date: January 8, 2009
    Applicant: StemCell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek
  • Publication number: 20080295618
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip.
    Type: Application
    Filed: August 11, 2008
    Publication date: December 4, 2008
    Applicant: StemCell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek