Patents Assigned to StemCell Technologies Inc.
  • Patent number: 10081793
    Abstract: The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: September 25, 2018
    Assignee: StemCell Technologies Inc.
    Inventor: Andy Isamu Kokaji
  • Patent number: 10017741
    Abstract: The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: July 10, 2018
    Assignee: STEMCELL TECHNOLOGIES INC.
    Inventors: Brenton John Short, Christopher Duronio
  • Patent number: 9970849
    Abstract: An improved method for the preparation of target specific surfaces and uses thereof is described. In particular, the surfaces are bound to mono-specific tetrameric antibody complexes prior to their addition to a sample containing target entities and separating them from non-target entities.
    Type: Grant
    Filed: January 21, 2015
    Date of Patent: May 15, 2018
    Assignee: StemCell Technologies Inc.
    Inventor: Andy Isamu Kokaji
  • Patent number: 9931633
    Abstract: A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 75 degrees and 110 degrees.
    Type: Grant
    Filed: January 10, 2014
    Date of Patent: April 3, 2018
    Assignee: STEMCELL TECHNOLOGIES INC.
    Inventors: Oliver Egeler, Steven Woodside
  • Patent number: 9708582
    Abstract: The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: July 18, 2017
    Assignee: Stemcell Technologies Inc.
    Inventors: Alexandra A. Blak, Sharon A. Louis, Michael John Riedel
  • Patent number: 9701935
    Abstract: A magnetic particle comprises a polysaccharide matrix and a plurality of magnetic crystals dispersed in the matrix. A method for making magnetic particles comprises combining a basic solution with a metal ion solution and allowing the metal ions to oxidize to form magnetic crystals, and combining the magnetic crystals with a polysaccharide solution to form the magnetic particles.
    Type: Grant
    Filed: April 16, 2009
    Date of Patent: July 11, 2017
    Assignee: StemCell Technologies Inc.
    Inventors: Steven M. Woodside, Graeme Milton, Jason Dowd
  • Patent number: 9683983
    Abstract: An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.
    Type: Grant
    Filed: May 3, 2012
    Date of Patent: June 20, 2017
    Assignee: StemCell Technologies Inc.
    Inventor: Steven M. Woodside
  • Patent number: 9290731
    Abstract: A filter apparatus comprises a filter having first and second opposed surfaces. A first reservoir is positioned adjacent with the first surface and in communication with the first surface, and a first inlet-outlet port is in communication with the first reservoir and spaced from the first surface. A second reservoir is positioned adjacent the second surface, and in communication with the second surface, and a second inlet-outlet port is in communication with the second reservoir and spaced from the second surface. A filter plate system comprises a reservoir plate. The reservoir plate comprises at least one reservoir well. The filter plate system further comprises a strainer plate. The strainer plate comprises at least one strainer well, which is removably receivable in the reservoir well. The strainer well comprises at least one mesh wall portion.
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: March 22, 2016
    Assignee: STEMCELL TECHNOLOGIES INC.
    Inventors: Jennifer Antonchuk, Cindy Miller
  • Patent number: 9273285
    Abstract: The present disclosure provides a method of determining the frequency and proliferative potential of progenitor cells in a starting cell preparation comprising (a) culturing the cell preparation for 5-10 days in a culture medium comprising FLT-3 Ligand (FL) and Thrombopoietin (TPO); (b) detecting and enumerating the colonies; and (c) assessing the frequency and proliferative potential of progenitor cells in the starting cell preparation based on the number of colonies in step (b). The number of colonies in step (b) correlates to the number of colonies in a standard 14-day colony-forming cell assay. The method is particularly useful for predicting and evaluating the suitability of grafts for transplantation.
    Type: Grant
    Filed: August 25, 2009
    Date of Patent: March 1, 2016
    Assignee: STEMCELL TECHNOLOGIES INC.
    Inventors: Albertus W. Wognum, Ning Yuan
  • Patent number: 9261454
    Abstract: A meniscus reducing member for use in a vessel for containing a liquid including a surface feature overlying at least a portion of an interior surface of the vessel. The surface feature includes at least two surfaces for contacting the liquid that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature.
    Type: Grant
    Filed: December 10, 2013
    Date of Patent: February 16, 2016
    Assignee: STEMCELL TECHNOLOGIES INC.
    Inventors: Oliver Egeler, Steven M. Woodside
  • Patent number: 9260684
    Abstract: A cell culture device comprises a well. A plurality of microwells are within the well, and a first common fluid volume is within the well above the microwells. A set of sub-microwells are within each microwell, and a second common fluid volume is within each microwell above the set of sub-microwells.
    Type: Grant
    Filed: November 10, 2011
    Date of Patent: February 16, 2016
    Assignee: Stemcell Technologies Inc.
    Inventors: Oliver Egeler, Steven Woodside
  • Publication number: 20160032248
    Abstract: The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided.
    Type: Application
    Filed: March 12, 2014
    Publication date: February 4, 2016
    Applicant: StemCell Technologies, Inc.
    Inventors: Brenton John SHORT, Christopher Duronio
  • Publication number: 20150352546
    Abstract: A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 75 degrees and 110 degrees.
    Type: Application
    Filed: January 10, 2014
    Publication date: December 10, 2015
    Applicant: Stemcell Technologies, Inc
    Inventors: Oliver Egeler, Steven Woodside
  • Patent number: 9062283
    Abstract: The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.
    Type: Grant
    Filed: February 26, 2008
    Date of Patent: June 23, 2015
    Assignee: STEMCELL TECHNOLOGIES INC.
    Inventors: Steven Woodside, Jason Dowd, Gary Dossantos, Oliver Egeler
  • Publication number: 20140168764
    Abstract: A meniscus reducing member for use in a vessel for containing a liquid including a surface feature overlying at least a portion of an interior surface of the vessel. The surface feature includes at least two surfaces for contacting the liquid that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature.
    Type: Application
    Filed: December 10, 2013
    Publication date: June 19, 2014
    Applicant: STEMCELL TECHNOLOGIES INC
    Inventors: Oliver Egeler, Steven M. Woodside
  • Publication number: 20140087360
    Abstract: An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.
    Type: Application
    Filed: May 3, 2012
    Publication date: March 27, 2014
    Applicant: Stemcell Technologies, Inc.
    Inventor: Steven M. Woodside
  • Publication number: 20130224857
    Abstract: The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.
    Type: Application
    Filed: March 14, 2013
    Publication date: August 29, 2013
    Applicant: STEMCELL TECHNOLOGIES INC.
    Inventor: STEMCELL TECHNOLOGIES INC.
  • Patent number: 8409873
    Abstract: A method of pipetting comprises (a) providing a pipette tip holder having a plurality of segregated compartments and a tip support member for supporting a plurality of pipette tips in the pipette tip holder, the tip support member configured to support a single pipette in tip each segregated compartment; (b providing a plurality of pipette tips in the tip support member; (c) removing a first pipette tip from a first segregated compartment; (d) using the first pipette tip to complete a pipetting operation and placing the first pipette tip into a segregated compartment that has not contained a used pipette tip; (e) subsequently removing the first pipette tip from the segregated compartment and using the first pipette tip to complete another pipetting operation; and, (f) replacing the first pipette tip into the pipette tip holder, whereby the first pipette tip, when placed in the segregated compartment, is segregated in the pipette tip holder to reduce the possibility of a fluid from the first pipette tip contac
    Type: Grant
    Filed: October 9, 2009
    Date of Patent: April 2, 2013
    Assignee: Stemcell Technologies Inc.
    Inventors: Steven Woodside, Terry Thomas, Graeme Cross, Andrew Angus
  • Publication number: 20110233148
    Abstract: A filter apparatus comprises a filter having first and second opposed surfaces. A first reservoir is positioned adjacent with the first surface and in communication with the first surface, and a first inlet-outlet port is in communication with the first reservoir and spaced from the first surface. A second reservoir is positioned adjacent the second surface, and in communication with the second surface, and a second inlet-outlet port is in communication with the second reservoir and spaced from the second surface. A filter plate system comprises a reservoir plate. The reservoir plate comprises at least one reservoir well. The filter plate system further comprises a strainer plate. The strainer plate comprises at least one strainer well, which is removably receivable in the reservoir well. The strainer well comprises at least one mesh wall portion.
    Type: Application
    Filed: December 16, 2009
    Publication date: September 29, 2011
    Applicant: STEMCELL TECHNOLOGIES INC.
    Inventors: Jennifer Antonchuk, Cindy Miller
  • Patent number: 7939031
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip. The seating surfaces are frustoconical outer surfaces. The pipette nozzle may be further configured to sealingly mate with and removably receive a third pipette tip having a third diameter different from the first diameter and the second diameter.
    Type: Grant
    Filed: August 11, 2008
    Date of Patent: May 10, 2011
    Assignee: Stemcell Technologies, Inc.
    Inventors: Andrew Angus, Adam Donath, Richard Grant