Patents Assigned to StemCell Technologies Inc.
  • Publication number: 20110233148
    Abstract: A filter apparatus comprises a filter having first and second opposed surfaces. A first reservoir is positioned adjacent with the first surface and in communication with the first surface, and a first inlet-outlet port is in communication with the first reservoir and spaced from the first surface. A second reservoir is positioned adjacent the second surface, and in communication with the second surface, and a second inlet-outlet port is in communication with the second reservoir and spaced from the second surface. A filter plate system comprises a reservoir plate. The reservoir plate comprises at least one reservoir well. The filter plate system further comprises a strainer plate. The strainer plate comprises at least one strainer well, which is removably receivable in the reservoir well. The strainer well comprises at least one mesh wall portion.
    Type: Application
    Filed: December 16, 2009
    Publication date: September 29, 2011
    Applicant: STEMCELL TECHNOLOGIES INC.
    Inventors: Jennifer Antonchuk, Cindy Miller
  • Patent number: 7939031
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip. The seating surfaces are frustoconical outer surfaces. The pipette nozzle may be further configured to sealingly mate with and removably receive a third pipette tip having a third diameter different from the first diameter and the second diameter.
    Type: Grant
    Filed: August 11, 2008
    Date of Patent: May 10, 2011
    Assignee: Stemcell Technologies, Inc.
    Inventors: Andrew Angus, Adam Donath, Richard Grant
  • Patent number: 7819029
    Abstract: There is also provided an apparatus for use on an automated pipette machine for transmitting pressure changes produced by a pump on the machine to a pipette nozzle. The apparatus comprises a housing defining a chamber a first conduit and a second conduit. The first conduit extends into the chamber and has a first opening positioned in the chamber. The first conduit is fluidically connectible to the nozzle. The second conduit extends into the chamber and has a first opening positioned in the chamber. The second conduit is fluidically connectible to the pump. The first opening of the first conduit is positioned above the first opening of the second conduit. The chamber defines at least a selected volume between the height of the first opening of the first conduit and the height of the first opening of the second conduit.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: October 26, 2010
    Assignee: Stemcell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek
  • Patent number: 7814805
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough and having at least two seating surfaces provided on the body. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to correspondingly receive, sealingly mate and provide passage first end fluid communication with a second size of pipette tip.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: October 19, 2010
    Assignee: Stemcell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek
  • Patent number: 7723047
    Abstract: The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD35 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f or CD24 and CD14. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f or CD24 and CD14, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated.
    Type: Grant
    Filed: June 6, 2007
    Date of Patent: May 25, 2010
    Assignees: Stemcell Technologies Inc., British Columbia Cancer Agency Branch
    Inventors: John Stingl, Connie J. Eaves
  • Publication number: 20100047845
    Abstract: The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.
    Type: Application
    Filed: February 26, 2008
    Publication date: February 25, 2010
    Applicant: STEMCELL TECHNOLOGIES INC.
    Inventors: Steven Woodside, Jason Dowd, Gary Dossantos, Oliver Egeler
  • Publication number: 20100024575
    Abstract: A method of pipetting comprises (a) providing a pipette tip holder having a plurality of segregated compartments and a tip support member for supporting a plurality of pipette tips in the pipette tip holder, the tip support member configured to support a single pipette in tip each segregated compartment; (b providing a plurality of pipette tips in the tip support member; (c) removing a first pipette tip from a first segregated compartment; (d) using the first pipette tip to complete a pipetting operation and placing the first pipette tip into a segregated compartment that has not contained a used pipette tip; (e) subsequently removing the first pipette tip from the segregated compartment and using the first pipette tip to complete another pipetting operation; and, (f) replacing the first pipette tip into the pipette tip holder, whereby the first pipette tip, when placed in the segregated compartment, is segregated in the pipette tip holder to reduce the possibility of a fluid from the first pipette tip contac
    Type: Application
    Filed: October 9, 2009
    Publication date: February 4, 2010
    Applicant: STEMCELL TECHNOLOGIES INC.
    Inventors: Steven Woodside, Terry Thomas, Graeme Cross, Andrew Angus
  • Patent number: 7638285
    Abstract: The present invention relates to an improved method that permits the differential isolation of mouse mammary stem cells and colony forming cells (CFCs). The method involves depletion of non-epithelial cells from freshly dissociated mouse mammary tissue by incubation with an antibody composition containing antibodies specific for CD45, Ter119, CD35 and optionally CD140a. After formation of conjugates between the non-epithelial mammary cells and the antibodies specific for CD45, Ter119, CD35 and optionally CD140a, the cell conjugates are removed and the remaining epithelial cells are then incubated with an antibody composition containing antibodies specific for CD24 and CD49f. After formation of conjugates between the epithelial cells and the antibodies specific for CD24 and CD49f, the mouse mammary stem and the luminal-restricted CFC cells can be differentially isolated. The invention also relates to kits for carrying out this method and to the cell preparations prepared by this method.
    Type: Grant
    Filed: June 7, 2006
    Date of Patent: December 29, 2009
    Assignees: StemCell Technologies Inc., British Columbia Cancer Agency Branch
    Inventors: John Stingl, Connie J. Eaves
  • Publication number: 20090007703
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip.
    Type: Application
    Filed: June 30, 2008
    Publication date: January 8, 2009
    Applicant: StemCell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek
  • Publication number: 20080295618
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip.
    Type: Application
    Filed: August 11, 2008
    Publication date: December 4, 2008
    Applicant: StemCell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek
  • Publication number: 20080264187
    Abstract: A pipette nozzle is provided for use on a movable arm on an automated pipette machine. The pipette nozzle includes a body defining a passage therethrough. At least two seating surfaces are provided on the body, including a first seating surface and a second seating surface. The first seating surface is configured to receive and sealingly mate with a first size of pipette tip in a manner such that the first end of the passage is in fluid communication with the first size of pipette tip. The second seating surface is configured to receive and sealingly mate with a second size of pipette tip in a manner such that the first end of the passage is in fluid communication with the second size of pipette tip.
    Type: Application
    Filed: June 30, 2008
    Publication date: October 30, 2008
    Applicant: StemCell Technologies Inc.
    Inventors: Andrew Angus, Fred Davis, Adam Donath, Richard Grant, Dirk Kurpershoek
  • Patent number: 7442545
    Abstract: A neural colony forming cell (NCFC) assay is described. The assay allows one to distinguish neural stem cells from neural progenitor cells. In one embodiment, the present invention provides a method for identifying neural stem cells or neural progenitor cells comprising: (a) suspending neural cells in a semi-solid medium which supports the growth of neural cells; (b) plating the cells in the semi-solid medium at a density that allows for the production of colonies; (c) culturing the plated cells until size differences can be discerned between the colonies; and (d) estimating colony size wherein the larger colonies are likely produced by neural stem cells and wherein the small colonies are likely produced by neural progenitor cells In alternate embodiments, NSC can be distinguished from neural progenitor cells by determining the morphology or antigen expression of the colonies.
    Type: Grant
    Filed: September 13, 2004
    Date of Patent: October 28, 2008
    Assignee: Stemcell Technologies, Inc.
    Inventors: Brent A. Reynolds, Sharon A. Louis
  • Patent number: 7387897
    Abstract: A method for preventing or inhibiting non-specific binding reactions between a detection reagent and an antigen in an immunological assay is described. The method involves using tetrameric antibody complexes that can bind to the antigen and are comprised of two monoclonal antibodies of a first animal species linked to two monoclonal antibodies of a second animal species that can bind the antibodies of the first animal species. Preferably, the antigen is an Fc Receptor and the method reduces the binding of a detection antibody with Fc receptors present on the surface of many cells.
    Type: Grant
    Filed: September 22, 2006
    Date of Patent: June 17, 2008
    Assignee: StemCell Technologies Inc.
    Inventors: Albertus Wernerus Wognum, Peter Michael Lansdorp
  • Patent number: 7316932
    Abstract: The present invention is related to a method for separating a first population of cells from a second population of cells in a sample by discontinuous density gradient separation using dense particles to target the first population of cells and a density separation medium (DSM) that is at least about 0.001 g/cm3 higher than the density of the second population of cells.
    Type: Grant
    Filed: October 1, 2002
    Date of Patent: January 8, 2008
    Assignee: Stemcell Technologies Inc.
    Inventor: Steven M. Woodside
  • Publication number: 20070026468
    Abstract: A method for preventing or inhibiting non-specific binding reactions between a detection reagent and an antigen in an immunological assay is described. The method involves using tetrameric antibody complexes that can bind to the antigen and are comprised of two monoclonal antibodies of a first animal species linked to two monoclonal antibodies of a second animal species that can bind the antibodies of the first animal species. Preferably, the antigen is an Fc Receptor and the method reduces the binding of a detection antibody with Fc receptors present on the surface of many cells.
    Type: Application
    Filed: September 22, 2006
    Publication date: February 1, 2007
    Applicant: StemCell Technologies Inc.
    Inventors: Albertus Wognum, Peter Lansdorp
  • Patent number: 7135335
    Abstract: The present invention relates to methods for separating cells using immunorosettes. The method involves contacting a sample containing nucleated cells and red blood cells with an antibody composition which allows immunorosettes of the nucleated cells and the red blood cells to form. The antibody composition preferably contains bifunctional antibodies or tetrameric antibody complexes.
    Type: Grant
    Filed: December 6, 2002
    Date of Patent: November 14, 2006
    Assignee: StemCell Technologies Inc.
    Inventors: Terry E. Thomas, Carrie E. Peters, Peter M. Lansdorp
  • Patent number: 7135340
    Abstract: A method for preventing or inhibiting non-specific binding reactions between a detection reagent and an antigen in an immunological assay is described. The method involves using tetrameric antibody complexes that can bind to the antigen and are comprised of two monoclonal antibodies of a first animal species linked to two monoclonal antibodies of a second animal species that can bind the antibodies of the first animal species. Preferably, the antigen is an Fc Receptor and the method reduces the binding of a detection antibody with Fc receptors present on the surface of many cells.
    Type: Grant
    Filed: November 7, 2002
    Date of Patent: November 14, 2006
    Assignee: Stemcell Technologies Inc.
    Inventors: Albertus Wernerus Wognum, Peter Michael Lansdorp
  • Patent number: 6872567
    Abstract: The present invention relates to methods for separating cells using immunorosettes. The method involves contacting a sample containing nucleated cells and red blood cells with an antibody composition which allows immunorosettes of the nucleated cells and the red blood cells to form. The antibody composition preferably contains bifunctional antibodies or tetrameric antibody complexes.
    Type: Grant
    Filed: July 12, 2002
    Date of Patent: March 29, 2005
    Assignee: StemCell Technologies Inc.
    Inventors: Terry E. Thomas, Carrie Peters, Peter Lansdorp
  • Publication number: 20040197904
    Abstract: The present invention relates to methods for separating cells using immunorosettes. The method involves contacting a sample containing nucleated cells and red blood cells with an antibody composition which allows immunorosettes of the nucleated cells and the red blood cells to form. The antibody composition preferably contains bifunctional antibodies or tetrameric antibody complexes.
    Type: Application
    Filed: April 15, 2004
    Publication date: October 7, 2004
    Applicant: StemCell Technologies Inc.
    Inventors: Terry Thomas, Carrie Peters, Peter Lansdorp
  • Patent number: 6750326
    Abstract: The present invention relates to methods for separating cells using immunorosettes. The method involves contacting a sample containing nucleated cells and red blood cells with an antibody composition which allows immunorosettes of the nucleated cells and the red blood cells to form. The antibody composition preferably contains bifunctional antibodies or tetrameric antibody complexes.
    Type: Grant
    Filed: April 2, 2001
    Date of Patent: June 15, 2004
    Assignee: Stemcell Technologies Inc.
    Inventors: Terry Thomas, Carrie Peters, Peter Lansdorp