Patents Assigned to Takara
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Patent number: 10760074Abstract: A composition for use in amplifying cDNA synthesized by a reverse transcription reaction and detecting RNA that serves as a template of the reverse transcription reaction, the composition containing a thermostable DNA polymerase, a thermostable ribonuclease H, and an intercalating dye. Since the composition of the present invention can suppress the influences to the nucleic acid amplification reaction by RNA that serves as a template for cDNA synthesis, the composition is useful in the detection of RNA, and more useful in quantification of RNA having a desired sequence by real-time RT-PCR.Type: GrantFiled: August 31, 2009Date of Patent: September 1, 2020Assignee: TAKARA BIO INC.Inventors: Kanako Usui, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
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Patent number: 10731226Abstract: A method for quantification of an adeno-associated virus genome, including the steps of (a) preparing a composition containing a sample, at least one primer pair for use in amplification of only a nucleotide sequence contained in inverted terminal repeats of an adeno-associated virus, and an intercalating dye; (b) performing nucleic acid amplification reaction using the composition prepared in the step (a); and (c) detecting an amplified product obtained in the step (b). The present invention is especially useful in the fields of medicine, gene engineering, and biology.Type: GrantFiled: November 13, 2018Date of Patent: August 4, 2020Assignee: TAKARA BIO INC.Inventors: Tamaki Akashika, Katsuyuki Dodo, Masanari Kitagawa
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Patent number: 10718014Abstract: The present invention provides miniaturized instruments for conducting chemical reactions where control of the reaction temperature is desired or required. Specifically, this invention provides chips and optical systems for performing and monitoring temperature-dependent chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost amplification of nucleic acids.Type: GrantFiled: January 12, 2018Date of Patent: July 21, 2020Assignee: Takara Bio USA, Inc.Inventors: Victor Joseph, Amjad Huda, Alnoor Shivji, Jie Zhou
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Patent number: 10655170Abstract: Methods of coupling adaptors to a target nucleic acid include coupling a first adaptor to a first end of the target nucleic acid to form a coupled first adaptor. A portion of a second adaptor is hybridized to a portion of the coupled first adaptor to form a hybridized second adaptor having a single-stranded 3?-end. The hybridized second adaptor is coupled to a second end of the target nucleic acid to form an adaptor-flanked product having at least a part of the first adaptor coupled to the first end of the target nucleic acid and at least a part of the second adaptor coupled to the second end of the target nucleic acid. These methods can minimize the formation of adaptor-dimers that may be problematic in subsequent complementary nucleic acid strand synthesis, amplification, and sequencing.Type: GrantFiled: July 5, 2017Date of Patent: May 19, 2020Assignee: Takara Bio USA, Inc.Inventors: Konstantinos Charizanis, Marta Gonzalez-Hernandez, Amanda McNulty, Karl Hecker, Emmanuel Kamberov, John Langmore
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Patent number: 10641772Abstract: The present disclosure provides methods, device, assemblies, and systems for dispensing and visualizing single cells. For example, provided herein are systems and methods for dispensing a dispense volume into a plurality of wells of a multi-well device, where, on average, a pre-determined number of cells (e.g., 1-20) are present in the dispense volume, and determining, via a cellular label, the number of cells present in each of the plurality of wells. Such dispensing and cell detection may be repeated a number of times with respect to wells identified as having less than the pre-determined number of cells in order increase the number wells in the multi-well device containing the desired number (e.g., a single cell).Type: GrantFiled: February 20, 2016Date of Patent: May 5, 2020Assignee: TAKARA BIO USA, INC.Inventors: Alain-Albert Mir, Thomas D. Schaal, Chun-Wah Lin, Shanavaz Loharasp D. Nasarabadi, Jude Dunne, Maithreyan Srinivasan, Patricio Espinoza
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Patent number: 10634668Abstract: Methods of reversibly inducing proximity of first and second target molecules in a sample are provided. Aspects of the methods include contacting the sample with a modifiable chemical inducer of proximity (MCIP) that reversibly induces proximity of the first and second target molecules, upon application of a stimulus that modifies the MCIP. Aspects of the invention further include methods for regulating a biological process in a cell. Aspects of the invention further include compositions, e.g., compounds and kits, etc., that find use in methods of the invention.Type: GrantFiled: September 10, 2013Date of Patent: April 28, 2020Assignee: TAKARA BIO USA, INC.Inventors: Eric Espinosa, Andrew Farmer
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Patent number: 10584363Abstract: Methods of producing single-stranded deoxyribonucleic acids (ssDNAs) are provided. Aspects of the methods include generating a double stranded deoxyribonucleic acid (dsDNA) and then selectively degrading one strand of the dsDNA to produce a ssDNA. ssDNAs produced using methods of the invention find use in a variety of applications, including genomic modification applications. Also provided are compositions, e.g., kits, that find use in practicing various embodiments of the invention.Type: GrantFiled: June 2, 2017Date of Patent: March 10, 2020Assignee: Takara Bio USA, Inc.Inventors: Hiroyuki Matsumoto, Michael Haugwitz, Andrew Farmer, Magnolia Bostick
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Publication number: 20200071675Abstract: The present invention relates to a method for producing endothelial cells, including carrying out: (a) inducing a population of mesoderm-lineage cells containing endothelial progenitor cells from pluripotent stem cells without forming an embryoid body; and (b) culturing the population of mesoderm-lineage cells containing endothelial progenitor cells in the presence of RepSox, in this order. According to the present invention, endothelial cells with high quality can be efficiently produced from pluripotent stem cells. The endothelial cells obtained by the method of the present invention are useful for the production of, for example, a myocardial sheet, and expected to be utilized in the treatment of a heart disease. A myocardial sheet can be produced by mixing the endothelial cells obtained by the method of the present invention with myocardial cells and mural cells and culturing the cells.Type: ApplicationFiled: December 1, 2017Publication date: March 5, 2020Applicant: TAKARA BIO INC.Inventors: Yuki YAMAMOTO, Tatsuji ENOKI, Yasuhiro TOSAKA, Yoko YAMAGUCHI, Junichi MINENO
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Publication number: 20200002384Abstract: The present invention provides: a mutant of adeno-associated virus (AAV) capsid protein, which contains at least one amino acid substitution in PLA2 domain when compared with the amino acid sequence for wild-type AAV capsid protein; a nucleic acid encoding the mutant; a cell containing the nucleic acid; a method for producing a recombinant AAV particle, comprising a step of culturing the cell to produce the recombinant AAV particle; a recombinant AAV particle containing the mutant; a composition containing the recombinant AAV particle; and a method for transferring a gene into a target cell, comprising a step of bringing the recombinant AAV particle into contact with the target cell.Type: ApplicationFiled: January 29, 2018Publication date: January 2, 2020Applicants: NIPPON MEDICAL SCHOOL FOUNDATION, NATIONAL CENTER OF NEUROLOGY AND PSYCHIATRY, TAKARA BIO INC.Inventors: Takashi OKADA, Hironori OKADA, Hiromi KINOH, Tatsuji ENOKI, Toshikazu NISHIE, Junichi MINENO
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Patent number: 10500585Abstract: The present invention provides methods, systems, assemblies, and articles for extracting restrained liquid (e.g., surface tension-restrained liquid) from open wells in a chip, where the restrained liquid does not flow out of the wells due to gravity when the wells are held upside down. For example, the present invention provides extraction fixtures that may be attached to, and/or held adjacent to, a chip such that any restrained liquid that is forced out of the open wells is collected by, or flows through, the extraction fixtures. Also for example, the present invention provides assemblies composed of a extraction fixture attached to, and/or held adjacent to, a chip, and methods of subjecting such assemblies to a force such that at least a portion of the restrained liquid in the open wells is forced out and collected by, or flows through, the extraction fixture.Type: GrantFiled: September 19, 2016Date of Patent: December 10, 2019Assignee: TAKARA BIO USA, INC.Inventors: Bradley L. Griswold, Syed A. Husain, Robert Moti, Wesley B. Dong, GoPaul Kotturi, Jude Dunne, Philip Lin
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Patent number: 10487313Abstract: The present invention relates to a method to differentiate pluripotent stem cells to a primitive streak cell population, in a stepwise manner for further maturation to definitive endoderm.Type: GrantFiled: September 15, 2017Date of Patent: November 26, 2019Assignees: Novo Nordisk A/S, Takara Bio Europe ABInventors: Nina Funa, Katja Hess, Jenny Ekberg, Henrik Semb
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Patent number: 10460553Abstract: A method of interaction between a machine and a player or a plurality of players, the method comprising the machine selecting a predetermined plurality of information bearing devices from a predetermined plurality of available information bearing devices as a selected plurality of information bearing devices upon initiation by a player. The machine organizing the selected plurality of information bearing devices for display on a visual display apparatus and the visual display apparatus displaying the selected plurality of information bearing devices as a set of selected information bearing devices in a in first orderly manner representing a first display order.Type: GrantFiled: December 21, 2017Date of Patent: October 29, 2019Assignee: Takara Gaming Solutions LimitedInventors: Yui Man Raymond Chan, Ka Lun Ernie Suek
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Patent number: 10421992Abstract: Provided are methods of depleting a target nucleic acid in a sample. The methods include contacting a target nucleic acid with two or more polymers that specifically hybridize to the target nucleic acid, and cleaving the hybridized regions of the target nucleic acid to deplete the target nucleic acid in the sample. Kits for practicing the subject methods are also provided.Type: GrantFiled: July 22, 2016Date of Patent: September 24, 2019Assignee: TAKARA BIO USA, INC.Inventors: Andrew Alan Farmer, Ian B. Oglesby, Magnolia Bostick, Steve Oh
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Patent number: 10415020Abstract: Provided is a method for producing non-enveloped viral particles, comprising a step for obtaining a fraction containing non-enveloped viral particles by removing precipitates which are produced in a step for adding a substance which reduces the solubility of proteins under acidic conditions and/or a substance which precipitates under acidic conditions to a neutral or basic sample containing non-enveloped viral particles, and acidifying the sample after the addition of the substance.Type: GrantFiled: January 8, 2016Date of Patent: September 17, 2019Assignee: TAKARA BIO INC.Inventors: Yasuhiro Kawano, Shuohao Huang, Tatsuji Enoki, Masanari Kitagawa
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Patent number: 10415087Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template nucleic acid, a template switch oligonucleotide, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template nucleic acid and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid including a region polymerized from the dNTPs by the polymerase. The methods further include attaching sequencing platform adapter constructs to ends of the product nucleic acid or a derivative thereof. Aspects of the invention further include compositions and kits.Type: GrantFiled: June 22, 2017Date of Patent: September 17, 2019Assignee: TAKARA BIO USA, INC.Inventors: Craig Betts, Andrew Alan Farmer
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Publication number: 20190218512Abstract: The present invention relates to the directed differentiation of mammalian pluripotent stem cells, especially human pluripotent stem (hPS) cells, into endodermal cells. In particular, the present invention relates to the treatment of mammalian pluripotent stem cells, especially hPS cells, with a DNA demethylating agent while undergoing differentiation into endodermal. The inventors have, as disclosed herein, found that exposing differentiating mammalian pluripotent stem cells, especially hPS cells, to a DNA demethylating agent leads to an improved morphology and improved yield of endodermal cells. The treatment with a DNA demethylating agent also leads to a significant down-regulation of expression of the stem cell marker Oct4 and to an improved expression of endoderm specific markers, notably sox17, cxcr4 and hhex.Type: ApplicationFiled: January 25, 2019Publication date: July 18, 2019Applicant: Takara Bio Europe ABInventors: Barbara Küppers-Munther, Josefina Edsbagge
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Patent number: 10336810Abstract: Disclosed herein are chimeric antigen receptors (CARs) comprising an intracellular segment comprising an interleukin receptor chain, a JAK-binding motif, a Signal Transducer and Activator of Transcription (STAT) 5 association motif and/or a CD3? intracellular signaling domain comprising an exogenous STAT3 association motif, as well as cells and 5 compositions comprising said CARs and uses thereof.Type: GrantFiled: February 11, 2016Date of Patent: July 2, 2019Assignees: University Health Network, Takara Bio Inc.Inventors: Shinya Tanaka, Naoto Hirano, Yuki Kagoya
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Publication number: 20190194609Abstract: The present invention relates to directed differentiation and maturation of hepatocyte-like cells. In particular, the present invention relates to exposure of hepatocyte-like cells to an activator of a retinoic acid responsive receptor, such as retinoic acid (RA), optionally in combination with an inhibitor of GSK-3 (Glycogen synthase kinase 3) or activator of Wnt signalling and/or with the overlay of the cells with one or more components characteristic of the mammalian extracellular matrix (matrix overlay). The present invention also relates to exposure of hepatocyte-like cells to an activator of a retinoic acid responsive receptor, such as retinoic acid (RA), optionally in combination with an inhibitor of a cycline dependent kinase (CDK) and/or with the overlay of the cells with one or more components characteristic of the mammalian extracellular matrix (matrix overlay).Type: ApplicationFiled: December 19, 2018Publication date: June 27, 2019Applicant: Takara Bio Europe ABInventors: Barbara Küppers-Munther, Josefina Edsbagge
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Publication number: 20190194613Abstract: The purpose of the present invention is to efficiently produce microglia from pluripotent stem cells. Provided is a method for producing microglia from pluripotent stem cells, comprising the following steps: (a) a step of co-culturing a pluripotent stem cell together with a feeder cell for 7 days or longer, and obtaining a blood progenitor cell; (b) a step of co-culturing the blood progenitor cell obtained in step (a) together with a feeder cell in the presence of IL-3 and/or GM-CSF, and obtaining an embryonic monocyte; and (c) a step of, in the presence of M-CSF, co-culturing the embryonic monocyte obtained in step (b) together with an astrocyte, or culturing the embryonic monocyte using an astrocyte supernatant.Type: ApplicationFiled: September 1, 2017Publication date: June 27, 2019Applicant: TAKARA BIO INC.Inventor: Hiroki SAITO
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Patent number: 10301660Abstract: Provided herein are compositions for and methods of generating ligation-competent nucleic acids. In some aspects, the compositions comprise Exonuclease III, T4 DNA Polymerase, Klenow, and/or T4 polynucleotide kinase.Type: GrantFiled: March 30, 2016Date of Patent: May 28, 2019Assignee: TAKARA BIO USA, INC.Inventors: Emmanuel Kamberov, John Langmore, Tim Tesmer, Marta Gonzalez-Plasky