Patents Assigned to Takara
  • Publication number: 20180362933
    Abstract: The present invention produces a large number of mesenchymal stem cells in a short time in a medium containing no heterologous components by including a step for culturing a cell population containing mesenchymal stem cells in the presence of a fibronectin fragment.
    Type: Application
    Filed: December 2, 2016
    Publication date: December 20, 2018
    Applicant: TAKARA BIO INC.
    Inventors: Kenichi TAHARA, Ikuei NUKAYA, Mitsuko IDENO, Saori YAMAGUCHI, Junichi MINENO
  • Patent number: 10155942
    Abstract: Methods of barcoding nucleic acids, such as genomic DNA, are provided herein. In some embodiments, a fragment of genomic DNA may comprise a first and a second barcode.
    Type: Grant
    Filed: November 5, 2013
    Date of Patent: December 18, 2018
    Assignee: Takara Bio USA, Inc.
    Inventors: Takao Kurihara, Emmanuel Kamberov, Tim Tesmer, John Langmore
  • Patent number: 10150985
    Abstract: Provided are methods of depleting a target nucleic acid from an initial collection of nucleic acids. Aspects of the methods include contacting the initial collection with a nucleic acid guided nuclease specific for the target nucleic acid in a manner sufficient to deplete the target nucleic acid from the initial collection. Depending on a given application, depletion of a target nucleic acid may vary, e.g., where depleting may include cleaving a target nucleic acid in, or selectively separating a target nucleic acid from, the initial collection of nucleic acids. Also provided are compositions and kits for practicing embodiments of the methods.
    Type: Grant
    Filed: December 23, 2014
    Date of Patent: December 11, 2018
    Assignee: Takara Bio USA, Inc.
    Inventors: Andrew Alan Farmer, Craig Betts, Nathalie Bolduc
  • Publication number: 20180346889
    Abstract: The present invention relates to a Thermus aquaticus (Taq) polymerase having a strand displacement activity in which an amino acid residue in a template DNA binding site of the DNA polymerase is substituted with an amino acid to increase a total charge in the site, a nucleic acid encoding the polymerase, a vector containing the nucleic acid, a transformant containing the vector containing the nucleic acid or the nucleic acid, a method for producing the polymerase, a method for amplifying nucleic acids utilizing the polymerase, and a kit containing the polymerase. According to the present invention, a DNA polymerase having a high thermostability, capable of efficiently replicating a long-strand of a template DNA, and having a strong strand displacement activity is provided.
    Type: Application
    Filed: November 24, 2016
    Publication date: December 6, 2018
    Applicants: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, TAKARA BIO INC.
    Inventors: Yoshizumi ISHINO, Sonoko ISHINO, Takeshi YAMAGAMI, Takashi UEMORI, Nariaki TAKATSU
  • Patent number: 10131890
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: November 20, 2018
    Assignee: TAKARA BIO INC.
    Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
  • Patent number: 10106777
    Abstract: Provided are improved methods using Glycogen synthase kinase 3 (GSK3) inhibitors by which endodermal cells, notably endodermal cells derived from human pluripotent stem cells (hPS), such as but not limited to hiPS-cells and hES-cells may be differentiated into hepatocyte like cells. The specific modulation of wingless integration gene (WNT)-signalling pathway and use of GSK3 inhibitors achieve direct differentiation and maturation of hepatocytes derived from human pluripotent stem (hPS) cells. GSK-3 inhibitors, when added to the growth medium at certain developmental stages, leads to more mature and functional features for the hepatocyte like cells as well as more pure and homogenous populations of hepatocyte like cells. Provided are also hepatocyte like cells obtained by these methods as well as compositions comprising them.
    Type: Grant
    Filed: July 7, 2016
    Date of Patent: October 23, 2018
    Assignee: Takara Bio Europe AB
    Inventors: Gabriella Brolén, Josefina Edsbagge
  • Patent number: 10093968
    Abstract: Provided are the following: a method, for improving reactivity of an acid synthesis reaction, comprising a step for adding an ?-amino acid to a reaction solution; a composition, for a nucleic acid synthesis reaction, comprising DNA polymerase, reaction buffer, at least one primer, at least one deoxyribonucleoside triphosphate, and an ?-amino acid; and a reaction buffer, for a nucleic acid synthesis reaction, comprising an ?-amino acid.
    Type: Grant
    Filed: May 23, 2017
    Date of Patent: October 9, 2018
    Assignee: TAKARA BIO INC.
    Inventors: Kiyoyuki Matsumura, Takashi Uemori, Hiroyuki Mukai
  • Publication number: 20180273907
    Abstract: Provided is a method for producing non-enveloped viral particles, comprising a step for obtaining a fraction containing non-enveloped viral particles by removing precipitates which are produced in a step for adding a substance which reduces the solubility of proteins under acidic conditions and/or a substance which precipitates under acidic conditions to a neutral or basic sample containing non-enveloped viral particles, and acidifying the sample after the addition of the substance.
    Type: Application
    Filed: January 8, 2016
    Publication date: September 27, 2018
    Applicant: TAKARA BIO INC.
    Inventors: Yasuhiro KAWANO, Shuohao HUANG, Tatsuji ENOKI, Masanari KITAGAWA
  • Patent number: 10072250
    Abstract: The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.
    Type: Grant
    Filed: July 10, 2014
    Date of Patent: September 11, 2018
    Assignee: TAKARA BIO INC.
    Inventors: Shuhei Sakamoto, Yasuhiro Kawano, Katsuyuki Dodo, Tatsuji Enoki, Hirofumi Yoshioka, Hikaru Takakura, Junichi Mineno
  • Patent number: 10023846
    Abstract: Provided are a production method for non-enveloped virus particles which is characterized in that a sample including non-envelopes virus particles is treated with PEG in at least two concentrations; a kit used in said production method; non-enveloped virus particles produced using said production method; and a pharmaceutical composition having the non-envelopes virus particles as an active ingredient.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: July 17, 2018
    Assignee: TAKARA BIO INC.
    Inventors: Toshikazu Nishie, Yasuhiro Kawano, Shuhei Sakamoto, Tatsuji Enoki, Hikaru Takakura, Junichi Mineno
  • Publication number: 20180163263
    Abstract: Provided are: a method with which it is possible to suppress nucleic-acid amplification of non-target nucleic acids and to selectively nucleic-acid amplify a target nucleic acid in a sample in which a large amount of non-target nucleic acids and a rare target nucleic acid are present in mixture; a method for detecting at high sensitivity rare mutant genes, the method combining this nucleic-acid amplification method and a specific real-time method for detecting the target nucleic acid; and a composition and kit for this method.
    Type: Application
    Filed: March 18, 2016
    Publication date: June 14, 2018
    Applicant: TAKARA BIO INC.
    Inventors: Takashi UEMORI, Takehiro SAGARA, Koichi INOUE
  • Patent number: 9995662
    Abstract: The present invention provides methods, systems, assemblies, and articles for capturing single cells with a polymer capture film. In certain embodiments, the polymer capture films comprise a plurality of individual channels with top and bottom openings, where the channels are dimensioned such that a single cell is: i) is captured inside the channel, partially or substantially occluding the channel, when negative pressure is provided to the bottom opening; or ii) is captured by the top opening, but does not enter the channel, when negative pressure is provided to the bottom opening. In some embodiments, the channels of the polymer capture film align with the wells of a multi-well chip such that the cell, or the contents of the single cell, may be transferred to a corresponding well.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: June 12, 2018
    Assignee: TAKARA BIO USA, INC.
    Inventors: Syed A. Husain, Bradley L. Griswold, Michael Slater, Patricio Espinoza, Jude Dunne, Glenn Hein, Maithreyan Srinivasan
  • Patent number: 9951381
    Abstract: Apparatus, systems, chips, and methods of performing a large number of simultaneous chemical reactions are provided herein. The chips of the invention comprise addressable units that can be addressed according to the temperature of the reaction to be run. The subject apparatus, systems, and chips are particularly suited for performing polymerase chain reactions on thousands of nucleic acid sequences, up to and including sequences of an entire genome of an organism of interest.
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: April 24, 2018
    Assignee: TAKARA BIO USA, INC.
    Inventors: Victor Joseph, Amjad Huda, Alnoor Shivji
  • Patent number: 9945850
    Abstract: Methods of determining whether a non-diagnostic analyte is present in a non-diagnostic sample are provided. Aspects of the methods include applying a non-diagnostic sample to a sample receiving region of a lateral flow assay device and reading a detection region to determine whether a non-diagnostic analyte is present in the non-diagnostic sample. Also provided are kits that find use in practicing methods of the invention.
    Type: Grant
    Filed: August 11, 2011
    Date of Patent: April 17, 2018
    Assignee: Takara Bio USA, Inc.
    Inventors: Dwayne Bisgrove, Hiroaki Sagawa
  • Patent number: 9938541
    Abstract: The invention provides an AAV particle containing an adeno-associated viral (AAV) capsid protein having an amino acid sequence selected from the group consisting of SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 24, and SEQ ID NO: 30 of the sequence listing; a nucleic acid that encodes this capsid protein; DNA containing this nucleic acid; a cell containing this DNA; and a method for producing this cell.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: April 10, 2018
    Assignee: TAKARA BIO INC.
    Inventors: Toshikazu Nishie, Fuyuko Takashima, Tatsuji Enoki, Junichi Mineno
  • Patent number: 9909171
    Abstract: The present invention provides miniaturized instruments for conducting chemical reactions where control of the reaction temperature is desired or required. Specifically, this invention provides chips and optical systems for performing and monitoring temperature-dependent chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost amplification of nucleic acids.
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: March 6, 2018
    Assignee: Takara Bio USA, Inc.
    Inventors: Victor Joseph, Amjad Huda, Alnoor Shivji, Jie Zhou
  • Patent number: 9895665
    Abstract: Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
    Type: Grant
    Filed: December 23, 2014
    Date of Patent: February 20, 2018
    Assignee: Takara Bio USA, Inc.
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Publication number: 20180002668
    Abstract: The present invention relates to a method to differentiate pluripotent stem cells to a primitive streak cell population, in a stepwise manner for further maturation to definitive endoderm.
    Type: Application
    Filed: September 15, 2017
    Publication date: January 4, 2018
    Applicants: Novo Nordisk A/S, Takara Bio Europe AB, Takara Bio Europe AB
    Inventors: Nina Funa, Katja Hess, Jenny Ekberg, Henrik Semb
  • Patent number: 9850498
    Abstract: The present invention provides an expression cassette comprising (1) an expression regulatory region having a sequence corresponding to a transcriptional activator-binding region and (2) a nucleic acid encoding a transcriptional activator which is capable of binding to the expression regulatory region, wherein the nucleic acid is operably linked to the expression regulatory region; a method for expressing a target product in a mammalian cell, a method for producing a cell expressing a target product, and a method for producing a target product in a mammalian cell using the expression cassette; and a kit comprising the expression cassette.
    Type: Grant
    Filed: December 10, 2013
    Date of Patent: December 26, 2017
    Assignee: TAKARA BIO INC.
    Inventors: Katsuyuki Dodo, Takahisa Tsukihara, Mayumi Shimomura, Koichi Inoue, Hideto Chono, Junichi Mineno, Masanari Kitagawa
  • Patent number: 9834584
    Abstract: Nucleic acid compositions encoding chromo/fluoroproteins and mutants thereof, as well as the encoded proteins, are provided. The subject proteins of interest are proteins that are colored and/or fluorescent, where this feature arises from the interaction of two or more residues of the protein. Also of interest are proteins that are substantially similar to, or mutants of, the above specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
    Type: Grant
    Filed: November 30, 2006
    Date of Patent: December 5, 2017
    Assignee: Takara Bio USA, Inc.
    Inventors: Sergey A. Lukyanov, Arcady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, Alexey Terskikh