Patents Assigned to Takara
  • Method for using heat-resistant mismatch endonuclease
    Patent number: 10294465
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: May 21, 2019
    Assignee: TAKARA BIO INC.
    Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
  • Maturation of hepatocyte-like cells derived from human pluripotent stem cells
    Patent number: 10294457
    Abstract: The present invention relates to directed differentiation and maturation of hepatocyte-like cells. In particular, the present invention relates to exposure of hepatocyte-like cells to an activator of a retinoic acid responsive receptor, such as retinoic acid (RA), optionally in combination with an inhibitor of GSK-3 (Glycogen synthase kinase 3) or activator of Wnt signalling and/or with the overlay of the cells with one or more components characteristic of the mammalian extracellular matrix (matrix overlay). The present invention also relates to exposure of hepatocyte-like cells to an activator of a retinoic acid responsive receptor, such as retinoic acid (RA), optionally in combination with an inhibitor of a cycline dependent kinase (CDK) and/or with the overlay of the cells with one or more components characteristic of the mammalian extracellular matrix (matrix overlay).
    Type: Grant
    Filed: November 28, 2013
    Date of Patent: May 21, 2019
    Assignee: Takara Bio Europe AB
    Inventors: Barbara Küppers-Munther, Josefina Edsbagge
  • Method for using heat-resistant mismatch endonuclease
    Patent number: 10280412
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: May 7, 2019
    Assignee: TAKARA BIO INC.
    Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
  • Multi-Z Imaging and Dispensing with Multi-Well Devices
    Publication number: 20190113457
    Abstract: Provided are methods, devices, assemblies, and systems for dispensing into the wells of a multi-well device and imaging such wells from multiple Z-planes. Multi-Z imaging of the present methods and systems may allow for the detection of wells of a multi-well device that contain a desired number of cells. Also provided are methods, devices, assemblies, and systems for processing cell-containing wells of a multi-well device identified through the use of multi-Z imaging.
    Type: Application
    Filed: July 20, 2017
    Publication date: April 18, 2019
    Applicant: TAKARA BIO USA, INC.
    Inventors: Patricio A. Espinoza Vallejos, Syed A. Husain, Chun-Wah LIN, Hermann HUBSCHLE
  • Balloon
    Patent number: 10252174
    Abstract: A balloon includes two joined plastic films. The balloon includes: a gas supply valve sandwiched between and joined to the first film and the second film; a first chamber receiving air supplied from the gas supply valve for expansion; and a second chamber communicating to the first chamber. The gas supply valve includes a gas passage that extends from a gas inlet positioned on an outer side of the second chamber to an inside of the first chamber through the second chamber. Part of the first film and the second film between the first chamber and the second chamber and between the gas passage and a peripheral edge of the balloon are joined together to form a first weld portion and a second weld portion. The first weld portion is formed on an opposite side of the second weld portion with the gas passage sandwiched therebetween.
    Type: Grant
    Filed: September 7, 2016
    Date of Patent: April 9, 2019
    Assignee: Takara Kosan Co., Ltd.
    Inventors: Takemi Morita, Mitsunori Yoshida, Akira Nakamura, Satoru Taniguchi, Takuya Ogura
  • METHOD FOR USING HEAT-RESISTANT MISMATCH ENDONUCLEASE
    Publication number: 20190100736
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Application
    Filed: December 12, 2018
    Publication date: April 4, 2019
    Applicant: TAKARA BIO INC.
    Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
  • METHOD FOR USING HEAT-RESISTANT MISMATCH ENDONUCLEASE
    Publication number: 20190085307
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Application
    Filed: September 26, 2018
    Publication date: March 21, 2019
    Applicant: TAKARA BIO INC.
    Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
  • Generation of pancreatic endoderm from pluripotent stem cells using small molecules
    Patent number: 10221392
    Abstract: A method of producing pancreatic cells or pancreatic cell precursors expressing at least 5% PDX1/NKX6.1 double positive, comprising exposing definitive endoderm cells to an effective amount of one or more small molecules, to differentiate the human definitive endoderm cells into the pancreatic cells or pancreatic cell precursors. The present invention also relates to pancreatic endoderm cells produced by said methods and uses of said pancreatic endoderm cells.
    Type: Grant
    Filed: July 21, 2016
    Date of Patent: March 5, 2019
    Assignees: Novo Nordisk A/S, TAKARA BIO EUROPE AB
    Inventors: Jenny Ekberg, Mattias Hansson, Ulrik Doehn, Katja Hess, Nina Funa
  • METHOD FOR QUANTIFYING ADENO-ASSOCIATED VIRUS
    Publication number: 20190062851
    Abstract: A method for quantification of an adeno-associated virus genome, including the steps of (a) preparing a composition containing a sample, at least one primer pair for use in amplification of only a nucleotide sequence contained in inverted terminal repeats of an adeno-associated virus, and an intercalating dye; (b) performing nucleic acid amplification reaction using the composition prepared in the step (a); and (c) detecting an amplified product obtained in the step (b). The present invention is especially useful in the fields of medicine, gene engineering, and biology.
    Type: Application
    Filed: November 13, 2018
    Publication date: February 28, 2019
    Applicant: TAKARA BIO INC.
    Inventors: Tamaki AKASHIKA, Katsuyuki DODO, Masanari KITAGAWA
  • DNA amplification and sequencing using DNA molecules generated by random fragmentation
    Patent number: 10214771
    Abstract: The present invention is directed to methods to prepare a DNA molecule or a plurality of DNA molecules by random fragmentation. In some embodiments, the present invention regards preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3? end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, a library is prepared with methods of the present invention.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: February 26, 2019
    Assignee: Takara Bio USA, Inc.
    Inventors: Vladimir L. Makarov, Irina Sleptsova, Emmanuel Kamberov, Eric Bruening
  • DNA POLYMERASE VARIANT
    Publication number: 20190055527
    Abstract: The present invention relates to a fusion polypeptide containing, in a direction of from an N-terminal side to a C-terminal side, one or more peptides which bind to a PCNA, and a polypeptide having a DNA polymerase activity; a method for amplifying nucleic acids using the polypeptide; and a composition and a kit, containing the polypeptide. According to the present invention, it is made possible to amplify a long-strand DNA in a short time in amplifying nucleic acids in the presence of PCNA even with a Pol I-type DNA polymerase.
    Type: Application
    Filed: November 24, 2016
    Publication date: February 21, 2019
    Applicants: KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, TAKARA BIO INC.
    Inventors: Yoshizumi ISHINO, Sonoko ISHINO, Takeshi YAMAGAMI, Takashi UEMORI, Nariaki TAKATSU
  • Compositions including a double stranded nucleic acid molecule and a stem-loop oligonucleotide
    Patent number: 10208337
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: February 19, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Systems and methods for whole genome amplification
    Patent number: 10208339
    Abstract: Provided herein are systems and methods for whole genome amplification and sequencing. In particular, provided herein are systems and methods for detection of nucleic acid variants (e.g., rare variants) in limited samples.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: February 19, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: Alain-Albert Mir, Thomas David Schaal, Jude Dunne, Maithreyan Srinivasan
  • Methods for producing mammalian pluripotent stem cell-derived endodermal cells
    Patent number: 10202579
    Abstract: The present invention relates to the directed differentiation of mammalian pluripotent stem cells, especially human pluripotent stem (hPS) cells, into endodermal cells. In particular, the present invention relates to the treatment of mammalian pluripotent stem cells, especially hPS cells, with a DNA demethylating agent while undergoing differentiation into endodermal. The inventors have, as disclosed herein, found that exposing differentiating mammalian pluripotent stem cells, especially hPS cells, to a DNA demethylating agent leads to an improved morphology and improved yield of endodermal cells. The treatment with a DNA de-methylating agent also leads to a significant down-regulation of expression of the stem cell marker Oct4 and to an improved expression of endoderm specific markers, notably sox17, cxcr4 and hhex.
    Type: Grant
    Filed: November 28, 2013
    Date of Patent: February 12, 2019
    Assignee: Takara Bio Europe AB
    Inventors: Barbara Küppers-Munther, Josefina Edsbagge
  • Kits including stem-loop oligonucleotides for use in preparing nucleic acid molecules
    Patent number: 10196686
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: February 13, 2017
    Date of Patent: February 5, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Spin columns comprising poly(acid) membrane separation matrices, and methods of making and using the same
    Patent number: 10195569
    Abstract: Spin columns that include a poly(acid) membrane separation matrix are provided. Also provided are kits that include the subject devices, as well as methods of using the devices, e.g., in sample preparation (such as protein purification) protocols.
    Type: Grant
    Filed: September 13, 2017
    Date of Patent: February 5, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: George G. Jokhadze, Sayantan Mitra
  • Method for using heat-resistant mismatch endonuclease
    Patent number: 10196618
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: February 5, 2019
    Assignee: TAKARA BIO INC.
    Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
  • MATURATION OF MAMMALIAN HEPATOCYTES
    Publication number: 20190024044
    Abstract: The present invention relates to directed differentiation and maturation of mammalian hepatocytes, such as human hepatocytes. The hepatocyte obtained in accordance with the present invention show a phenotype which is more similar to that of primary hepatocytes than previously shown. In particular, the present invention relates to exposure of mammalian hepatocytes, such as human hepatocytes, to at least one maturation factor selected from the group consisting of Src kinase inhibitors, vitamin D including precursors, metabolites and analogs thereof, hypoxia inducing compounds, sphingosine and sphingosine derivatives, activators of peroxisome proliferator-activated receptors (PPARs), platelet-activating factor (PAF), PKC inhibitors, and combinations thereof.
    Type: Application
    Filed: June 3, 2016
    Publication date: January 24, 2019
    Applicant: Takara Bio Europe AB
    Inventor: Barbara Kuppers-Munther
  • METHOD FOR USING HEAT-RESISTANT MISMATCH ENDONUCLEASE
    Publication number: 20190017037
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Application
    Filed: September 26, 2018
    Publication date: January 17, 2019
    Applicant: TAKARA BIO INC.
    Inventors: Kiyoyuki MATSUMURA, Nariaki TAKATSU, Takashi UEMORI, Hiroyuki MUKAI
  • Method for quantifying adeno-associated virus
    Patent number: 10161011
    Abstract: A method for quantification of an adeno-associated virus genome, including the steps of (a) preparing a composition containing a sample, at least one primer pair for use in amplification of only a nucleotide sequence contained in inverted terminal repeats of an adeno-associated virus, and an intercalating dye; (b) performing nucleic acid amplification reaction using the composition prepared in the step (a); and (c) detecting an amplified product obtained in the step (b). The present invention is especially useful in the fields of medicine, gene engineering, and biology.
    Type: Grant
    Filed: November 27, 2014
    Date of Patent: December 25, 2018
    Assignee: TAKARA BIO INC.
    Inventors: Tamaki Akashika, Katsuyuki Dodo, Masanari Kitagawa