Patents Assigned to Temasek Life Sciences Laboratory
  • VIRUS INDUCED GENE SILENCING (VIGS) FOR FUNCTIONAL ANALYSIS OF GENES IN COTTON
    Publication number: 20120096595
    Abstract: The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.
    Type: Application
    Filed: June 10, 2010
    Publication date: April 19, 2012
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Jian Ye, Nam Hai Chua, Jing Qu, Yun Feng Geng, Yun Ping Bu
  • JATROPHA CURCAS CURCIN GENES, TISSUE-SPECIFIC PROMOTERS AND GENERATION OF CURCIN-DEFICIENT TRANSGENIC JATROPHA PLANTS
    Publication number: 20120073018
    Abstract: The present invention relates to the isolation of Jatropha curcas curcin genes and tissue-specific promoters and to the production of curcin-deficient Jatropha plants. More specifically, the present invention relates to the isolation of Jatropha curcas Curcin 1, Curcin 2 and Curcin 2 A. The present invention further relates to of the Curcin 1, Curcin 2 A and Curcin 2 genes and more particularly to tissue specific promoters of the Curcin 1 and Curcin 2A genes. The present invention further relates to production of curcin-deficient transgenic jatropha plants by using RNAi technology to suppress curcin gene expression.
    Type: Application
    Filed: June 2, 2010
    Publication date: March 22, 2012
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Zhong Chao Yin, Li Fang Wu, Hui Zhu Mao, Cheng Xiang Qiu
  • Transposition of maize AC/DS elements in vertebrates
    Patent number: 8137974
    Abstract: The present invention is directed to the use of the maize Ac/Ds transposable elements in vertebrates.
    Type: Grant
    Filed: May 11, 2006
    Date of Patent: March 20, 2012
    Assignee: Temasek Life Sciences Laboratory Limited
    Inventors: Sergey Parinov, Alexander Emelyanov
  • Biomolecule Surface Display and Uses Thereof
    Publication number: 20120045473
    Abstract: A vaccine for the treatment or prevention of a disease in a subject, wherein said disease is associated with an avian influenza virus, and wherein said vaccine comprises an expression vector comprising a nucleic acid encoding a hemagglutinin peptide, such that in use said hemagglutinin peptide is expressed by said expression vector in said subject.
    Type: Application
    Filed: May 5, 2006
    Publication date: February 23, 2012
    Applicant: Temasek Life Sciences Laboratory Limited
    Inventor: Jimmy Kwang
  • DIGOXIN-LIKE FUNGAL GLYCOSIDE WITH CYTOTOXIC PROPERTIES: NOVEL ASSAY AND APPLICATIONS
    Publication number: 20120003261
    Abstract: The present invention provides methods of isolating cytotoxic metabolites from a fungus, and specific metabolites obtained from such methods. The present invention also provides methods of controlling fungal diseases in plants by treating the plants with cytotoxic metabolites as well as methods of treating cardiac arrhythmia in organisms in need of such treatment by administering the above-noted metabolites to such organisms.
    Type: Application
    Filed: June 18, 2009
    Publication date: January 5, 2012
    Applicant: Temasek Life Sciences Laboratory Limited
    Inventors: Rajesh Narhari Patkar, Naweed Isaak Naqvi
  • FUNCTIONAL ANALYSIS OF JATROPHA CURCAS GENES
    Publication number: 20110265223
    Abstract: The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).
    Type: Application
    Filed: December 16, 2009
    Publication date: October 27, 2011
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Jian Ye, Nam Hai Chua, JIng Qu
  • Monoclonal Antibodies Specific to the Fusion Peptide From Hemagglutinin From Influenza A Viruses and Uses Thereof
    Publication number: 20110256141
    Abstract: This invention relates to methods and products for the diagnosis, surveillance, prevention, and treatment of influenza A virus infections in animals and humans. More particularly, the invention relates to antibodies and related binding proteins for the detection, prevention and treatment of influenza A viruses. The monoclonal antibodies and related binding proteins of the invention are useful for the treatment of the highly pathogenic H5 subtypes of avian influenza virus (AIV).
    Type: Application
    Filed: December 24, 2008
    Publication date: October 20, 2011
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Prabakaran Mookkan, Nayana Prabhu Padubidhri, Sumathy Velumani, Hwei-Sing Jimmy Kwang
  • GENETIC TRANSFORMATION OF JATROPHA CURCAS
    Publication number: 20110247099
    Abstract: The present invention relates to methods for the regeneration and Agrobacterium-mediated transformation of plants in the genera of Jatropha, more specifically, in Jatropha curcas.
    Type: Application
    Filed: December 15, 2009
    Publication date: October 6, 2011
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Hui Zhu Mao, Jian Ye, Nam Hai Chua
  • Fluorescent primer system for detection of nucleic acids (Q priming)
    Patent number: 7964716
    Abstract: The present invention is directed to a self-quenching primer comprising; a fluorophore that can be quenched by guanine; an oligonucleotide sequence that forms a hairpin; an oligonucleotide that is a target specific sequence and; use in amplification reactions, particularly in polymerase chain reactions, during which the fluorophore is released thereby emitting fluorescence.
    Type: Grant
    Filed: December 5, 2006
    Date of Patent: June 21, 2011
    Assignee: Temasek Life Sciences Laboratory Limited
    Inventors: Xiao Kun Liu, Yan Hong
  • METHOD OF HIGH FREQUENCY REGENERATION OF SORGHUM
    Publication number: 20110124107
    Abstract: The present invention relates generally to the regeneration of sorghum involving organogenesis. More specifically, the present invention relates to a method of regenerating sorghum, particularly Sorghum bicolor (L.) Moench, via organogenesis that yields a high frequency of regenerants. In addition to providing high frequency regeneration, the present invention can be applied directly to the production of sorghum variants through somoclonal variation and to the genetic transformation of sorghum.
    Type: Application
    Filed: July 23, 2008
    Publication date: May 26, 2011
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Ramesh Anbazhagan Vasudevan, Kumar Nadimuthu, Srinivasan Ramachandran
  • SOMATIC EMBRYOGENESIS OF JATROPHA CURCAS FROM OVULES
    Publication number: 20110117652
    Abstract: The present invention relates to the field of somatic embryo production, particularly to methods for somatic embyrogenesis of Jatropha from ovules. More specifically, the present invention relates to a method and media compositions for somatic embryogenesis of Jatropha curcas from ovules of unopened flower buds. The method is well suited for Jatropha curcas transformation, for producing clonal planting stock useful for large scale Jatropha curcas plantation and for producing haploids, double haploids, diploids and disease-free plantlets.
    Type: Application
    Filed: June 18, 2009
    Publication date: May 19, 2011
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Ramesh Anbazhagan Vasudevan, Srinivasan Ramachandran
  • Binding Protein and Epitope-Blocking Elisa for the Universal Detection of H5-Subtype Influenza Viruses
    Publication number: 20110053142
    Abstract: Monoclonal antibodies and related binding proteins specific to influenza H5 subtype HA protein can be used in serological diagnosis of influenza H5 infection in mammalian and avian serum samples, including human serum samples. Each antibody reacts strongly with a wide variety of strains of H5 subtype and does not show cross-reactivity with non-H5 influenza subtypes.
    Type: Application
    Filed: February 5, 2008
    Publication date: March 3, 2011
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Prabakaran Mookkan, Nayana Prabhu Padubidhri, Sumathy Velumani, Hwei-Sing Jimmy Kwang
  • Methods for high efficiency transformation and regeneration of plant suspension cultures
    Patent number: 7897838
    Abstract: The present invention provides methods for high efficiency plant transformation via Agrobacterium-mediated T-DNA conjugation to suspension-cultured cells or calli. The methods described herein employ membranes or filters as porous solid support for the co-culture of T-DNA donor and recipient.
    Type: Grant
    Filed: April 20, 2004
    Date of Patent: March 1, 2011
    Assignee: Temasek Life Sciences Laboratory Limited
    Inventors: Lianghui Ji, Cai Lin
  • Production of Homogeneous Cell Line Highly Permissive To Porcine Circovirus Type 2 (PCV2) Infection
    Publication number: 20110008871
    Abstract: Continuous cell lines that are highly permissive to infection by porcine circovirus type 2 (“PCV2”) are described. PCV2 is the causal agent of post-weaning multi-systemic wasting syndrome (“PMWS”) in pigs. PMWS has emerged as a major disease that poses a significant threat to the economics of global swine industry. The highly permissive cell lines of this invention provide efficient and reliable sources of PCV2 for use in development of vaccines, therapies and diagnostic agents for PMWS.
    Type: Application
    Filed: May 11, 2007
    Publication date: January 13, 2011
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Adeline Hui Ling Lau, Jennifer Siew Kee Lau, Hwei-Sing Kwang
  • H5 Subtype-Specific Binding Proteins Useful for H5 Avian Influenza Diagnosis and Surveillance
    Publication number: 20110003278
    Abstract: The invention provides monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes of avian influenza virus (“AIV”). The monoclonal antibodies and related binding proteins are useful for the detection of H5 subtypes of AIV, including the pathogenic H5N1 subtypes. Virus may be detected in formalin preserved, paraffin embeded specimens as well as frozen specimens and biological fluids. Accordingly, the invention provides means for the diagnosis and surveillance of dangerous viral infections.
    Type: Application
    Filed: May 11, 2007
    Publication date: January 6, 2011
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Yuen Fern Ho, Qing Yun Du, Fang He, Jimmy Hwei-Sing Kwang
  • REGENERATION AND MASS PROPAGATION OF JATROPHA CURCAS THROUGH SOMATIC EMBRYOGENESIS
    Publication number: 20100304488
    Abstract: The present invention relates to the field of somatic embryo production, particularly to methods for the regeneration of Jatropha through somatic embryogenesis. More specifically, the present invention relates to a method and media compositions for regeneration of plants of Jatropha curcas. The method is well suited for Jatropha curcas transformation and for producing clonal planting stock useful for large scale Jatropha curcas plantation.
    Type: Application
    Filed: January 7, 2009
    Publication date: December 2, 2010
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Lin Cai, Lianghui Ji
  • Monoclonal Antibodies Specific to Hemagglutinin from Influenza Virus H5-Subtype and Uses Thereof
    Publication number: 20100297126
    Abstract: Monoclonal antibodies and related binding proteins that are specific for conformational epitopes of avian influenza virus K5 subtype hemagglutinin glycoprotein are provided. The antibodies can be used for the detection and treatment of H5 subtype AIV in specimens.
    Type: Application
    Filed: August 26, 2008
    Publication date: November 25, 2010
    Applicants: TEMASEK LIFE SCIENCES LABORATORY LIMITED, REPUBLIC OF INDONESIA
    Inventors: Hong Liang Qian, Fang He, Siti Fadilah Supari, Hwei-Sing Kwang
  • N-TERMINAL XA27 SIGNAL ANCHOR AND ITS USE FOR LOCALIZATION OF FUSION PROTEINS
    Publication number: 20100299787
    Abstract: The present application is directed to a functional signal anchor that localizes a fusion protein to the apoplast of vascular elements in plants. The signal anchor is useful for engineering secretory proteins to the cell wall and or/apoplast of plant cells. The signal anchor is also useful for producing secretory proteins in transgenic plant cells in a bioreactor.
    Type: Application
    Filed: November 15, 2007
    Publication date: November 25, 2010
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Zhongchao Yin, Lifang Wu
  • NOVEL PLANT HOMEODOMAIN PROTEIN-ENCODING GENES AND THEIR USES
    Publication number: 20100293662
    Abstract: A group of genes including GhCIR1 from cotton (Gossypium hirsutum), and AtCIR1 and AtCIR2 from Arabidopsis thaliana promote shoot regeneration in plants even in the absence of cytokinin. In the presence of cytokinin, the genes significantly improve transformation efficiency. The genes can be used as an enhancer as well as a selectable marker of transformation in plants. The proteins encoded by the novel genes have a homeodomain (HD) at the N-terminus and a highly divergent domain at the C-terminus. The proteins share a common structural motif.
    Type: Application
    Filed: February 13, 2006
    Publication date: November 18, 2010
    Applicant: TEMASEK LIFE SCIENCES LABORATORY
    Inventors: Lianghui Ji, Lin Cai, Nam-Hai Chua
  • LEXA-BASED CHEMICAL-INDUCIBLE GENE EXPRESSION SYSTEM FOR USE IN TRANSGENIC ANIMALS
    Publication number: 20100269182
    Abstract: The present invention relates generally to chemical-inducible system and to methods of use in transgenic animals. More specifically, the present invention relates to a chimeric transcription factor that binds to a ligand and functions in ligand-dependent manner to induce expression of genes of interest under the control of a synthetic operator-promoter sequence. The expression of genes of interest can be tightly controlled by adding or removing the ligand.
    Type: Application
    Filed: July 2, 2008
    Publication date: October 21, 2010
    Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED
    Inventors: Alexander Emelyanov, Sergey Parinov, Nam-Hai Chua