Abstract: The present invention relates to the field of plant molecular biology, more particularly cotton WRINKLED 1-like (WREL) genes. More specifically, the present invention relates to cotton WRIL genes whose products act as transcription factors of genes involved in fatty acid biosynthesis. The present invention also relates to methods of increasing cotton fiber length in cotton. In one embodiment, the methods involve modulating the level of activity of an enzyme involved in a fatty acid biosynthesis in the host cotton cell and/or culturing the host cotton cell. In another embodiment, the methods involve the manipulation of transcription factors which can regulate an enzyme involved in fatty acid biosynthesis.
Type:
Application
Filed:
April 19, 2012
Publication date:
March 12, 2015
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Abstract: The present invention relates to the field of modifying Jatropha branching, flowering time, fruiting and seed setting. More specifically, the present invention relates to a method for control and optimization of Jatropha curcas flowering time, fruiting and seed production by manipulating one gene from Jatropha. The present invention can be used in various Jatropha breeding efforts.
Type:
Application
Filed:
February 27, 2013
Publication date:
January 29, 2015
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Inventors:
Nam-Hai Chua, Jian Ye, Yun-Feng Geng, Bipei Zhang
Abstract: The present invention relates to the isolation of Jatropha curcas curcin genes and tissue-specific promoters and to the production of curcin-deficient Jatropha plants. More specifically, the present invention relates to the isolation of Jatropha curcas Curcin 1, Curcin 2 and Curcin 2 A. The present invention further relates to of the Curcin 1, Curcin 2 A and Curcin 2 genes and more particularly to tissue specific promoters of the Curcin 1 and Curcin 2A genes. The present invention further relates to production of curcin-deficient transgenic jatropha plants by using RNAi technology to suppress curcin gene expression.
Type:
Grant
Filed:
June 2, 2010
Date of Patent:
January 13, 2015
Assignee:
Temasek Life Sciences Laboratory Liimited
Abstract: The present invention relates to murine monoclonal antibodies C, F and H which target major neutralizing epitopes of influenza H5 hemagglutinin of clade 2.3 and active fragments thereof. The present invention also relates to methods and compositions for the prophylaxis and treatment of H5N1 influenza using murine monoclonal antibodies C, F or H or active fragments thereof. The present invention additionally relates to methods and compositions for providing universal protection against H5 influenza viruses using murine monoclonal antibodies C, F or H or fragments thereof together with complementary murine monoclonal antibody or active fragments thereof. The present invention further relates to methods and compositions for the characterization and quantification of H5 expression using these murine monoclonal antibody or fragments thereof.
Type:
Application
Filed:
January 12, 2012
Publication date:
October 23, 2014
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Abstract: The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.
Type:
Grant
Filed:
June 10, 2010
Date of Patent:
October 7, 2014
Assignee:
Temasek Life Sciences Laboratory Limited
Inventors:
Jian Ye, Nam Hai Chua, Jing Qu, Yun Feng Geng, Yun Ping Bu
Abstract: The present invention relates generally to chemical-inducible system and to methods of use in transgenic animals. More specifically, the present invention relates to a chimeric transcription factor that binds to a ligand and functions in ligand-dependent manner to induce expression of genes of interest under the control of a synthetic operator-promoter sequence. The expression of genes of interest can be tightly controlled by adding or removing the ligand.
Type:
Grant
Filed:
July 2, 2008
Date of Patent:
October 7, 2014
Assignee:
Temasek Life Sciences Laboratory Limited
Inventors:
Alexander Emelyanov, Sergey Parinov, Nam-Hai Chua
Abstract: An expression cassette, wherein said expression cassette comprises: (a) an ie 1 promoter from white spot syndrome virus or a variant thereof; (b) a nucleic acid sequence encoding an N-terminal gp64 signal peptide or variant thereof, a nucleic acid sequence encoding an antigenic peptide, a nucleic acid encoding a transmembrane region, and a nucleic acid encoding a gp64 cytoplasmic region or variant thereof; in which the promoter is operably linked to the nucleic acid sequence.
Type:
Application
Filed:
February 8, 2012
Publication date:
May 8, 2014
Applicant:
Temasek Life Sciences Laboratory Limited
Inventors:
Premanand Balraj, Hwei-Sing Jimmy Kwang, Anbu Kumar Karuppannan
Abstract: The present invention relates to the application of isolated promoters and synthetic dominant selection constructs and enhancers for gene targeting for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the Rhodosporidium, Sporisorium, Sporobolomyces or Ustilago genera.
Type:
Application
Filed:
May 10, 2012
Publication date:
April 24, 2014
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Inventors:
Liang Hui Ji, Yan Bin Liu, John Chong Mei Koh, Long Hua Sun
Abstract: The present invention relates to the field of plant molecular biology, more particularly Jatropha microsomal co6 oleate desaturases. The present invention also relates to Jatropha plants or plants of other oil crops having seeds with altered ratios of monosaturated and polyunsaturated fats. In particular, the present invention relates to Jatropha plants or plants of other oil crops where the plants exhibit elevated levels of oleic acid.
Type:
Application
Filed:
September 28, 2011
Publication date:
April 3, 2014
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Abstract: The invention provides monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes of avian influenza virus (“AIV”). The monoclonal antibodies and related binding proteins are useful for the detection of H5 subtypes of AIV, including the pathogenic H5N1 subtypes. Virus may be detected in formalin preserved, paraffin embedded specimens as well as frozen specimens and biological fluids. Accordingly, the invention provides for the diagnosis and surveillance of dangerous viral infections.
Type:
Grant
Filed:
September 13, 2012
Date of Patent:
January 28, 2014
Assignee:
Temasek Life Sciences Laboratory Limited
Inventors:
Yuen Fern Ho, Qing Yun Du, Fang He, Jimmy Hwei-Sing Kwang
Abstract: The invention provides monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes of avian influenza virus (“AIV”). The monoclonal antibodies and related binding proteins are useful for the detection of H5 subtypes of AIV, including the pathogenic H5N1 subtypes. Virus may be detected in formalin preserved, paraffin embedded specimens as well as frozen specimens and biological fluids. Accordingly, the invention provides for the diagnosis and surveillance of dangerous viral infections.
Type:
Grant
Filed:
September 13, 2012
Date of Patent:
January 28, 2014
Assignee:
Temasek Life Sciences Laboratory Limited
Inventors:
Yuen Fern Ho, Qing Yun Du, Fang He, Jimmy Hwei-Sing Kwang
Abstract: A group of genes including GhCIR1 from cotton (Gossypium hirsutum), and AtCIR1 and AtCIR2 from Arabidopsis thaliana promote shoot regeneration in plants even in the absence of cytokinin. In the presence of cytokinin, the genes significantly improve transformation efficiency. The genes can be used as an enhancer as well as a selectable marker of transformation in plants. The proteins encoded by the novel genes have a homeodomain (HD) at the N-terminus and a highly divergent domain at the C-terminus. The proteins share a common structural motif.
Abstract: Monoclonal antibodies and related binding proteins specific to influenza H5 subtype HA protein can be used in serological diagnosis of influenza H5 infection in mammalian and avian serum samples, including human serum samples. Each antibody reacts strongly with a wide variety of strains of H5 subtype and does not show cross-reactivity with non-H5 influenza subtypes.
Type:
Grant
Filed:
February 5, 2008
Date of Patent:
November 5, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Abstract: Monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes or neuraminidase glycoprotein of N1 subtypes of avian influenza virus (AIV) are provided. The monoclonal antibodies and related binding proteins are useful for the detection of H5 and N1 subtypes of AIV, including H5N1 subtypes and provide means for the diagnosis, surveillance and treatment of dangerous viral infections.
Type:
Grant
Filed:
September 11, 2012
Date of Patent:
November 5, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Inventors:
Hong Liang Qian, Fang He, Hwei-Sing Kwang
Abstract: Monoclonal antibodies and related binding proteins that are specific for conformational epitopes of avian influenza virus H5 subtype hemagglutinin glycoprotein are provided. The antibodies can be used for the detection and treatment of H5 subtype AIV in specimens.
Type:
Application
Filed:
May 20, 2013
Publication date:
September 26, 2013
Applicants:
NATIONAL INSTITUTE OF HEALTH RESEARCH AND DEVELOPMENT (NIHRD)-MINISTRY OF HEALTH, Temasek Life Sciences Laboratory Limited
Abstract: Monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes or neuraminidase glycoprotein of N1 subtypes of avian influenza virus (AIV) are provided. The monoclonal antibodies and related binding proteins are useful for the detection of H5 and N1 subtypes of AIV, including H5N1 subtypes and provide means for the diagnosis, surveillance and treatment of dangerous viral infections.
Type:
Grant
Filed:
September 11, 2012
Date of Patent:
September 24, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Inventors:
Hong Liang Qian, Fang He, Hwei-Sing Kwang
Abstract: The invention provides monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes of avian influenza virus (“AIV”). The monoclonal antibodies and related binding proteins are useful for the detection of H5 subtypes of AIV, including the pathogenic H5N1 subtypes. Virus may be detected in formalin preserved, paraffin embedded specimens as well as frozen specimens and biological fluids. Accordingly, the invention provides for the diagnosis and surveillance of dangerous viral infections.
Type:
Grant
Filed:
May 11, 2007
Date of Patent:
September 24, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Inventors:
Yuen Fern Ho, Qing Yun Du, Fang He, Jimmy Hwei-Sing Kwang
Abstract: This invention relates to methods and products for the diagnosis, surveillance, prevention, and treatment of influenza A virus infections in animals and humans. More particularly, the invention relates to antibodies and related binding proteins for the detection, prevention and treatment of influenza A viruses. The monoclonal antibodies and related binding proteins of the invention are useful for the treatment of the highly pathogenic H5 subtypes of avian influenza virus (AIV).
Type:
Grant
Filed:
December 24, 2008
Date of Patent:
September 24, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Abstract: Methods of isolating biologically active molecules from an organism, for example from a fungus, are described. The methods involve isolating an active molecule which possesses a specific biological activity on, in or against wild-type yeast cells, but not on, in or against yeast cells that express a given ABC transporter from a given organism, including but not limited to Magnaporthe species such as Magnaporthe grisea. In preferred embodiments, the biological activity is antifungal activity. In most preferred embodiments, the antifungal activity is against a human pathogen, including but not limited to Candida albicans. The biologically active molecules can be formulated as pharmaceutical compositions and used to treat fungal infections in a subject, including a human subject.
Type:
Application
Filed:
February 8, 2013
Publication date:
August 29, 2013
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Inventor:
Temasek Life Sciences Laboratory Limited
Abstract: The present invention provides nucleic acids and methods for conferring resistance to bacterial disease in plants. The present invention also provides promoters and promoter sequences useful for controlling expression in transgenic plants.
Type:
Application
Filed:
September 6, 2010
Publication date:
August 29, 2013
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Inventors:
Zhong Chao Yin, Ke Yu Gu, Dong Sheng Tian