Abstract: Continuous cell lines that are highly permissive to infection by porcine circovirus type 2 (“PCV2”) are described. PCV2 is the causal agent of post-weaning multi-systemic wasting syndrome (“PMWS”) in pigs. PMWS has emerged as a major disease that poses a significant threat to the economics of global swine industry. The highly permissive cell lines of this invention provide efficient and reliable sources of PCV2 for use in development of vaccines, therapies and diagnostic agents for PMWS.
Type:
Grant
Filed:
May 11, 2007
Date of Patent:
August 27, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Abstract: The present invention is directed to fish whose genome has integrated therein an oncogenic nucleic acid operably linked to a promoter. Methods of making the fish and methods for their use are also provided. The fish may advantageously be utilized in methods of screening for drugs or agents that modulate oncogene-mediated neoplastic or hyperplasic transformation, or that modulate sensitivity to chemotherapy or radiation therapy. Immortal tumor cells lines, methods of making immortal tumor cells lines and methods of their use are also provided.
Type:
Grant
Filed:
June 26, 2012
Date of Patent:
August 20, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Abstract: The present invention relates to the murine monoclonal antibody 4C2 or to chimeric or humanized monoclonal antibodies specific to a major neutralizing epitope of influenza H5 hemagglutinin and active fragments thereof. The present invention also relates to methods and compositions for the prophylaxis and treatment of H5N1 influenza using such murine or chimeric or humanized monoclonal antibodies or fragments thereof.
Type:
Application
Filed:
August 23, 2010
Publication date:
August 8, 2013
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Inventors:
Prabakaran Mookkan, Fang He, Hwei-Sing Jimmy Kwang
Abstract: Monoclonal antibodies and related binding proteins that are specific for conformational epitopes of avian influenza virus K5 subtype hemagglutinin glycoprotein are provided. The antibodies can be used for the detection and treatment of H5 subtype AIV in specimens.
Type:
Grant
Filed:
August 26, 2008
Date of Patent:
May 21, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Abstract: The present invention relates generally to the regeneration of sorghum involving organogenesis. More specifically, the present invention relates to a method of regenerating sorghum, particularly Sorghum bicolor (L.) Moench, via organogenesis that yields a high frequency of regenerants. In addition to providing high frequency regeneration, the present invention can be applied directly to the production of sorghum variants through somoclonal variation and to the genetic transformation of sorghum.
Type:
Grant
Filed:
July 23, 2008
Date of Patent:
April 30, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Abstract: The present invention provides methods of isolating cytotoxic metabolites from a fungus, and specific metabolites obtained from such methods. The present invention also provides methods of controlling fungal diseases in plants by treating the plants with cytotoxic metabolites as well as methods of treating cardiac arrhythmia in organisms in need of such treatment by administering the above-noted metabolites to such organisms.
Abstract: The present invention relates to the field of reverse genetics. More particularly, the present invention relates to a novel reverse genetic approach termed “fragment switch” which is used to generate an allelic series in genes of interest which are useful for functional analysis.
Type:
Application
Filed:
March 24, 2011
Publication date:
February 28, 2013
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Abstract: Monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes or neuraminidase glycoprotein of N1 subtypes of avian influenza virus (AIV) are provided. The monoclonal antibodies and related binding proteins are useful for the detection of H5 and N1 subtypes of AIV, including H5N1 subtypes and provide means for the diagnosis, surveillance and treatment of dangerous viral infections.
Type:
Grant
Filed:
September 12, 2008
Date of Patent:
February 26, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Inventors:
Hong Liang Qian, Fang He, Hwei-Sing Kwang
Abstract: The present application is directed to a functional signal anchor that localizes a fusion protein to the apoplast of vascular elements in plants. The signal anchor is useful for engineering secretory proteins to the cell wall and or/apoplast of plant cells. The signal anchor is also useful for producing secretory proteins in transgenic plant cells in a bioreactor.
Type:
Grant
Filed:
November 15, 2007
Date of Patent:
February 5, 2013
Assignee:
Temasek Life Sciences Laboratory Limited
Abstract: Monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes or neuraminidase glycoprotein of N1 subtypes of avian influenza virus (AIV) are provided. The monoclonal antibodies and related binding proteins are useful for the detection of H5 and N1 subtypes of AIV, including H5N1 subtypes and provide means for the diagnosis, surveillance and treatment of dangerous viral infections.
Type:
Application
Filed:
September 11, 2012
Publication date:
January 3, 2013
Applicant:
Temasek Life Sciences Laboratory Limited
Inventors:
Hong Liang Qian, Fang He, Hwei-Sing Kwang
Abstract: Monoclonal antibodies and related binding proteins that bind specifically to the envelope glycoprotein of H5 subtypes or neuraminidase glycoprotein of N1 subtypes of avian influenza virus (AIV) are provided. The monoclonal antibodies and related binding proteins are useful for the detection of H5 and N1 subtypes of AIV, including H5N1 subtypes and provide means for the diagnosis, surveillance and treatment of dangerous viral infections.
Type:
Application
Filed:
September 11, 2012
Publication date:
January 3, 2013
Applicant:
Temasek Life Sciences Laboratory Limited
Inventors:
Hong Liang Qian, Fang He, Hwei-Sing Kwang
Abstract: The present invention generally provides a method to generate broad-spectrum resistance to bacterial blight disease in plants. More specifically, the present invention provides a method to generate broad-spectrum resistance to Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight disease of rice, and enhanced resistance to Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak of rice. Xa27, an inducible bacterial blight R gene in rice, was induced by the cognate avrXa27 gene expressed in host. Rice plants carrying the avrXa27 transgene and wild-type Xa27 gene conferred resistance to incompatible and compatible pathogens, and enhanced resistance to X. oryzae pv. oryzicola strain L8. The Xa27-mediated enhanced resistance to X. oryzae pv. oryzicola was also observed in the interaction between IRBB27 and L8 harboring pHM1avrXa27. This was further verified by the fact that the Xa27 gene in IRBB27 was induced by the avrXa27 gene in bacteria.
Abstract: The present invention is directed to fish whose genome has integrated therein an oncogenic nucleic acid operably linked to a promoter. Methods of making the fish and methods for their use are also provided. The fish may advantageously be utilized in methods of screening for drugs or agents that modulate oncogene-mediated neoplastic or hyperplasic transformation, or that modulate sensitivity to chemotherapy or radiation therapy Immortal tumor cells lines, methods of making immortal tumor cells lines and methods of their use are also provided.
Type:
Application
Filed:
June 26, 2012
Publication date:
October 25, 2012
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Abstract: The present invention relates to methods for the regeneration and Agrobacterium-mediated transformation of plants in the genera of Jatropha, more specifically, in Jatropha curcas.
Type:
Application
Filed:
July 3, 2012
Publication date:
October 25, 2012
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Abstract: The present invention relates to the field of the production of transgenic plants through Agrobacterium-mediated transformation of cells of somatic embryogenic calli or embryogenic suspension cultures and regeneration of the transformed cells into fruit-setting plants. In particular, the present invention relates to the production of transgenic plants in the Euphorbiaceae family. The present invention further relates to media compositions, selection methods and engineered Agrobacterium tumefaciens strains that improve Agrobacterium-mediated transformation efficiency.
Type:
Application
Filed:
December 4, 2009
Publication date:
September 27, 2012
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Inventors:
Lin Cai, Longhua Sun, Lin Fu, Lianghui Ji
Abstract: The present invention relates to the field of controlling pests, such as insects, using a virus to express pest genes in hosts. More specifically, the present invention relates to a method for rapidly screening for pest genes which can lead to mortality of the pest when the pest has ingested host tissues expressing virus-linked pest gene sequences. The present invention also relates to a method for controlling pests by viral expression of target pest sequences to modify endogenous expression of pest genes in cells or tissues of the pest.
Type:
Application
Filed:
September 15, 2010
Publication date:
September 20, 2012
Applicant:
TEMASEK LIFE SCIENCES LABORATORY LIMITED
Inventors:
Jian Ye, Nam-Hai Chua, Jing Qu, Shi-Qiang Gao
Abstract: The present invention is directed to fish whose genome has integrated therein an oncogenic nucleic acid operably linked to a promoter. Methods of making the fish and methods for their use are also provided. The fish may advantageously be utilized in methods of screening for drugs or agents that modulate oncogene-mediated neoplastic or hyperplastic transformation, or that modulate sensitivity to chemotherapy or radiation therapy. Immortal tumor cells lines, methods of making immortal tumor cell lines and methods of their use are also provided.
Type:
Grant
Filed:
July 18, 2006
Date of Patent:
August 14, 2012
Assignee:
Temasek Life Sciences Laboratory Limited
Abstract: In Magnaporthe species and other plant pathogenic fungal species, the appressoriu, (infection structure) is responsible for breaching the host plant cell wall and gaining entry into the host tissues. Magnaporthe ABC3 protein is an MDR transporter that plays an important role during host penetration and also is involved in regulating fungal response to intracellular oxidative stress. The insertional mutant abc3?, in which the ABC3 efflux pump function is blocked, lacks functional appressoria and is therefore incapable of causing disease in host plants. This invention provides the abc3 nucleic acid (gene) and ABC3 protein from Magnaporthe or from Aspergillus, Ustilago or Fusarium and describes methods for reducing plant pathogenicity for important rice pathogens.