Abstract: The disclosure is directed to methods and compositions for screening a library of aptamers for aptamers having a binding affinity to a target molecule. The methods and compositions described herein utilize a throughput approach that is able to simultaneously measure binding affinity and link the binding affinity to the identity (e.g., sequence) of the aptamer.

Abstract: A conformal graphene-encapsulated battery electrode material is formed by: (1) coating a battery electrode material with a metal catalyst to form a metal catalyst-coated battery electrode material; (2) growing graphene on the metal catalyst-coated battery electrode material to form a graphene cage encapsulating the metal catalyst-coated battery electrode material; and (3) at least partially removing the metal catalyst to form a void inside the graphene cage.

Abstract: Methods are provided for treating a subject with a therapeutic dose of anti-CD47 agent by administering a primer agent prior to administering a therapeutically effective dose of an anti-CD47 agent to the subject.

Abstract: Methods are provided for treating a subject with a therapeutic dose of anti-CD47 agent by administering a primer agent prior to administering a therapeutically effective dose of an anti-CD47 agent to the subject.

Abstract: Provided herein are compositions, methods, and kits for proliferating muscle cells by exposing the muscle cells to a prostaglandin E2 (PGE2) compound or compound that activates PGE2 signaling. Also provided are methods for regenerating muscle in a subject suffering from muscular atrophy, dystrophy, and/or injury by administering a PGE2 compound alone or in combination with isolated muscle cells. The PGE2 compound in combination with the isolated muscle cells can be administered prophylactically to prevent a muscle disease or condition.

Abstract: Systems and methods for multi-view cooperative contrastive self-supervised learning, may include receiving a plurality of video sequences, the video sequences comprising a plurality of image frames; applying selected images of a first and second video sequence of the plurality of video sequences to a plurality of different encoders to derive a plurality of embeddings for different views of the selected images of the first and second video sequences; determining distances of the derived plurality of embeddings for the selected images of the first and second video sequences; detecting inconsistencies in the determined distances; and predicting semantics of a future image based on the determined distances.

Abstract: Compositions and methods are provided for induction and maintenance of quiescence of stem cells.

Abstract: The present disclosure relates to the field of G protein coupled receptor (GPCR) structural biology and signaling. In particular, the present disclosure relates to binding domains directed against and/or specifically binding to GPCR:G protein complexes. Also provided are nucleic acid sequences encoding such binding domains and cells expressing or capable of expressing such binding domains. The binding domains of the present disclosure can be used as universal tools for the structural and functional characterization of G-protein coupled receptors in complex with downstream heterotrimeric G proteins and bound to various natural or synthetic ligands, for investigating the dynamic features of G protein activation, as well as for screening and drug discovery efforts that make use of GPCR:G protein complexes.

Abstract: Compositions and methods for treating hyperinsulinemic hypoglycemia, such as hyperinsulinemic hypoglycemia after bariatric surgery, are provided. In some embodiments, an effective amount of the glucagon-like peptide-1 receptor antagonist exendin(9-39) is subcutaneously administered twice per day.

Abstract: Medical energy devices, systems, and methods that allows for treatment of systemic inflammatory disorders through modulation of neural activity.

Abstract: Compositions for treating ocular disease are disclosed herein. In some embodiments, the composition comprises a dynamic hydrogel comprising a polymer and a plurality of nanoparticles, wherein the polymer is non-covalently crosslinked with the plurality of nanoparticles. The dynamic hydrogel can also comprise an ocular therapeutic encapsulated by the dynamic hydrogel.

Abstract: A method for diagnostic imaging reconstruction uses a prior image xpr from a scan of a subject to initialize parameters of a neural network which maps coordinates in image space to corresponding intensity values in the prior image. The parameters are initialized by minimizing an objective function representing a difference between intensity values of the prior image and predicted intensity values output from the neural network. The neural network is then trained using subsampled (sparse) measurements of the subject to learn a neural representation of a reconstructed image. The training includes minimizing an objective function representing a difference between the subsampled measurements and a forward model applied to predicted image intensity values output from the neural network. Image intensity values output from the trained neural network from coordinates in image space input to the trained neural network are computed to produce predicted image intensity values.

Abstract: The present disclosure provides systems and methods for analyzing a mixed population of cells. In particular, the present disclosure provides systems and methods for digital cytometry of a biological sample, digital analysis of a biological sample, digital purification of a biological sample, evaluation of a disease in an individual, and prediction of a clinical outcome of a disease therapy.

Abstract: The present disclosure presents a general approach to engineering existing protein-protein interactions through domain addition and evolution. The disclosure teaches the creation of novel fusion proteins that include knottin peptides where a portion of the knottin peptide is replaced with a sequence that has been created for binding to a particular target. Such fusion proteins can also be bispecific or multi specific in that they can bind to and/or inhibit two or more receptors or receptor ligands. Knottins may be fused with an existing ligand (or receptor) as a general platform tor increasing the affinity of a ligand-receptor interaction or for creating a multi specific protein. In addition, the fusion proteins may comprise a knottin peptide fused to another protein where the other protein facilitates proper expression and folding of the knottin.

Abstract: Devices, methods, and kits are provided for allergy testing based on basophil activation in a blood sample. Susceptibility of an individual to an allergic reaction to an allergen is detected by collecting a blood sample from the individual and assaying for activation of basophils in response to stimulation with an allergen. In particular, a microfluidic device is provided for automating the assay for detecting basophil activation as an indication of the susceptibility of an individual to an allergic reaction as well as kits containing such a device and diagnostic methods for using such a device for allergy testing. Additionally, such a microfluidic device can be adapted for multiplexed detection of allergic responses to multiple allergens by performing assays in parallel to detect the basophil responses to each allergen.

Abstract: A hybrid analog-digital electronic circuit for solving non-linear programming problems includes an analog circuit and a digital microcontroller interconnected to each other by an analog-to-digital converter (ADC) and a digital-to-analog converter (DAC). The analog circuit physically realizes a nonlinear programming problem (NLP) where voltages in the analog circuit represent variables in the NLP, and the interconnection of the analog circuit components enforce Karush-Kuhn-Tucker (KKT) conditions on the variables, such that the voltages in the analog circuit that represent the variables of the NLP naturally converge to an optimal and feasible solution of the NLP.

Abstract: The present invention provides a clinically applicable method of stem cell transplantation that facilitates engraftment and reconstitutes immunocompetence of the recipient without requiring radiotherapy or chemotherapy, and without development of GVHD or graft rejection. Aspects of the present invention are based on the discovery that the depletion of the endogenous stem cell niche facilitates efficient engraftment of stem cells into that niche. In particular, the present invention combines the use of selective ablation of endogenous stem cells with a combination of antibodies specific for CD117, and agents that modulate immunoregulatory signaling pathways, e.g. agonists of immune costimulatory molecules, in combination with the administration to the recipient of exogenous stem cells, resulting in efficient, long-term engraftment, even in immunocompetent recipients.

Abstract: A system and method for analyzing a plurality of tissue image samples at different stages of image processing in order to establish the level of fibrosis within a patient from which the tissue image sample was retrieved. Various embodiments incorporate machine learning techniques which enable an automated progressive approach to effectively and efficiently diagnosing fibrotic conditions based on tissue image samples.

Abstract: A vertically selective liftoff scribing process is provided. One application is the fabrication of solar cells and solar modules. The basis of this technology is absorption of an indirectly focused laser beam in the front electrode material of the device, which enables removal of this layer (e.g., a P1 scribe) or removal of layers above the front electrode while leaving the front electrode intact (e.g., a P2 or P3 scribe). The laser fluence can be selected to choose between these alternatives, and further fine tuning is possible depending on details of the device structure.

Abstract: A consolidated metallic glass structure is formed by fabricating [200] metallic glass nanoparticles with a solution-phase synthesis that provides coated metallic glass nanoparticles with a polymer ligand layer; stripping [202] the polymer ligand layer from the coated metallic glass nanoparticles to provide bare metallic glass nanoparticles; depositing [204] the bare metallic glass nanoparticles on a substrate to provide a deposited structure; and sintering [206] the deposited structure with heat and/or pressure to provide the consolidated metallic glass structure. The metallic glass nanoparticles are preferably composed substantially of nickel and boron, iron and boron, or cobalt and boron.